Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_L21
(806 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 84 9e-17
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 79 3e-15
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 77 2e-14
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 75 7e-14
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 69 5e-12
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 61 7e-10
At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger) fa... 38 0.010
At3g23980.1 68416.m03012 dentin sialophosphoprotein-related cont... 30 1.6
At4g38350.1 68417.m05422 patched family protein similar to SP|O1... 30 2.1
At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG... 30 2.1
At1g42470.1 68414.m04897 patched family protein similar to SP|O1... 29 2.7
At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family... 29 2.7
At5g43130.1 68418.m05265 transcription initiation factor IID (TF... 29 3.6
At4g03420.1 68417.m00469 expressed protein 29 4.8
At1g06490.1 68414.m00688 glycosyl transferase family 48 protein ... 29 4.8
At3g59140.1 68416.m06593 ABC transporter family protein putative... 28 6.3
At3g18290.1 68416.m02326 zinc finger protein-related weak alignm... 28 6.3
At2g37730.1 68415.m04627 fringe-related protein similarity to pr... 28 6.3
At5g39440.1 68418.m04777 Snf1-related protein kinase, putative s... 28 8.4
At5g38260.1 68418.m04612 serine/threonine protein kinase, putati... 28 8.4
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 84.2 bits (199), Expect = 9e-17
Identities = 63/211 (29%), Positives = 99/211 (46%), Gaps = 6/211 (2%)
Frame = -2
Query: 706 RLVCFLMLLCYVVAKKKT--EDHKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKV 536
+LV F + + + K E KV++ +YYESLCP + FI +L V+ D + V
Sbjct: 9 KLVFFACFVLFTFSHKLVTGESDKVELNLYYESLCPGCQSFIVDELVKVFDSDLDTITDV 68
Query: 535 KMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTS 356
K+VP+G + KV + IC HG +EC N ++ACV+ + + + C+ N T
Sbjct: 69 KLVPFG---YAKVSNNLTVICQHGEEECKLNALEACVINTLP-NPKSQYKFIRCVENNT- 123
Query: 355 PDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKY 176
D +CL + C +S+ L+ Y +T ++ FVP V IN
Sbjct: 124 -DNWESSCL---KGYGNEKAINDCYNSDLSKKLILGYAKQTSSLKPKHEFVPWVTIN--- 176
Query: 175 DKDVETQAFENLKAVVCRV---ATPQPSICS 92
K + T+ ++L VC+ TP P CS
Sbjct: 177 SKPLYTK-LDDLVGQVCKAYKGKTPLPIDCS 206
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 79.4 bits (187), Expect = 3e-15
Identities = 57/211 (27%), Positives = 99/211 (46%), Gaps = 6/211 (2%)
Frame = -2
Query: 706 RLVCF--LMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKV 536
+LV F L+LL + + KVK+ +YYESLCP ++FI L ++ D + +
Sbjct: 8 KLVFFGCLLLLTFTDNLVAGKSGKVKLNLYYESLCPGCQEFIVDDLGKIFDYDLYTITDL 67
Query: 535 KMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTS 356
K+ P+G + ++ + C HG +EC N ++AC L+ Q ++ + C+ + T
Sbjct: 68 KLFPFGNA---ELSDNLTVTCQHGEEECKLNALEACALRTWPDQKSQ-YSFIRCVESDT- 122
Query: 355 PDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKY 176
K ++C V + C + + L+ Y KT + P +VP V +N K
Sbjct: 123 --KGWESC---VKNSGREKAINDCYNGDLSRKLILGYATKTKNLKPPHEYVPWVTLNGK- 176
Query: 175 DKDVETQAFENLKAVVCRV---ATPQPSICS 92
D Q+ ++L A +C T P +C+
Sbjct: 177 PLDDSVQSTDDLVAQICNAYKGKTTLPKVCN 207
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 76.6 bits (180), Expect = 2e-14
Identities = 51/176 (28%), Positives = 90/176 (51%), Gaps = 2/176 (1%)
Frame = -2
Query: 643 KVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKMVPYGKSTHDKVDGKWSFICHH 467
KV + +YYESLCP FI LA ++ D +V + + P+G +T + D + +C H
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWG-NTKLRSDNV-TAVCQH 95
Query: 466 GADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDKSLDTCLTQVDKMSESDK-LK 290
GA EC+ + V+AC + D + ++ + C+ + K D T +K++ + K +
Sbjct: 96 GAFECFLDTVEACAI-DAWPKVSDHFPFIYCVEKLVTEHK-YDKWETCYEKLNLNSKPVA 153
Query: 289 RCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEKYDKDVETQAFENLKAVVCR 122
C SS G+ L Y +T+A+ P +VP V++ D + +EN + +C+
Sbjct: 154 DCLSSGHGNELALHYAAETNALQPPHKYVPWVVV----DGQPLYEDYENFISYICK 205
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 74.5 bits (175), Expect = 7e-14
Identities = 54/191 (28%), Positives = 89/191 (46%), Gaps = 6/191 (3%)
Frame = -2
Query: 646 HKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKMVPYGKSTHDKVDGKWSFICH 470
+KVKI +YYESLCP + FI L ++ D + +K+VP+G + + + C
Sbjct: 37 NKVKINLYYESLCPYCQNFIVDDLGKIFDSDLLKITDLKLVPFGNA---HISNNLTITCQ 93
Query: 469 HGADECYGNKVQACVLKDRNLQDTE-KMEIVICLMNQTSPDKSLDTCLTQVDKMSESDKL 293
HG +EC N ++AC + R L D + + + + C+ T+ ++C V K +
Sbjct: 94 HGEEECKLNALEACGI--RTLPDPKLQYKFIRCVEKDTN---EWESC---VKKSGREKAI 145
Query: 292 KRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIINEK--YDKDVETQAFENLKAVVCRV 119
C + + L+ Y T ++ +VP V +N K YD + NL A VC+
Sbjct: 146 NDCYNGDLSQKLILGYAKLTSSLKPKHEYVPWVTLNGKPLYDN------YHNLVAQVCKA 199
Query: 118 --ATPQPSICS 92
P +CS
Sbjct: 200 YKGKDLPKLCS 210
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 68.5 bits (160), Expect = 5e-12
Identities = 45/178 (25%), Positives = 83/178 (46%), Gaps = 4/178 (2%)
Frame = -2
Query: 706 RLVCFLMLLCYVVAKKKT---EDHKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVK 539
+LV F LL + + E KVK+ +YYESLCP + FI L ++ D +
Sbjct: 12 KLVFFACLLLFTFSSHNLVAGESDKVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITD 71
Query: 538 VKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQT 359
+K++P+G + V + C HG +EC N ++AC ++ Q + + C+ T
Sbjct: 72 LKLIPFGNA---HVSDDLTVTCQHGEEECKLNALEACAIRTWPNQRLH-YKFIRCVETNT 127
Query: 358 SPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIIN 185
+ + ++C V K + C + + L+ Y ++T ++ +VP + +N
Sbjct: 128 N---AWESC---VKKYGGEKAINDCYNGDLSKELILGYANQTLSLKPEHKYVPWMTLN 179
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 61.3 bits (142), Expect = 7e-10
Identities = 42/179 (23%), Positives = 78/179 (43%), Gaps = 1/179 (0%)
Frame = -2
Query: 718 MKTLRLVCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLV 542
M + + +C + + KV +++YYE+LCP +FI +L ++ +
Sbjct: 1 MASYQRLCITSCTIFFCLLSLSSSQKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSI 60
Query: 541 KVKMVPYGKSTHDKVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQ 362
+++VP+G + + DG + +C HG EC N + AC + D K I Q
Sbjct: 61 DLQLVPWGNAA-IRPDG--TILCQHGEAECALNAIHACAI--NAYPDVMKHFGYIYCTEQ 115
Query: 361 TSPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASYGDKTDAVMRPLAFVPTVIIN 185
+ L+ ++ + S C + G+ L Y ++T + FVP V++N
Sbjct: 116 LVLENKLEKWADCLEMVGLSRAAVDCYINGYGNQLEQRYAEETSELYPAHRFVPWVVVN 174
>At2g44580.1 68415.m05548 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 624
Score = 37.5 bits (83), Expect = 0.010
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = -2
Query: 502 KVDGKWSFICHHGADECYGNKVQACVLKDRNLQDTEKMEIVICLMNQTSPDKSLDTCLTQ 323
++DG W I + D + CVLKD + D ++ E+V L+ P + CL
Sbjct: 194 EIDGFWRVIDENYLDVILRMLLHNCVLKDWSFDDLDEDEVVNALVADEFPSQLASHCLRV 253
Query: 322 V-DKMSESDKLK 290
K++E+DK K
Sbjct: 254 FGSKVNETDKWK 265
>At3g23980.1 68416.m03012 dentin sialophosphoprotein-related
contains weak similarity to Dentin sialophosphoprotein
precursor (Swiss-Prot:Q9NZW4) [Homo sapiens]
Length = 736
Score = 30.3 bits (65), Expect = 1.6
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -3
Query: 567 SGGTSEDLSKSKWSRMGRARTTRWMGNG 484
SGG S DLSK+ SR + +T +GNG
Sbjct: 525 SGGKSTDLSKNSTSRKNVSTSTEGLGNG 552
>At4g38350.1 68417.m05422 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (PID:g2276463); contains Pfam profile PF02460
Patched family
Length = 1064
Score = 29.9 bits (64), Expect = 2.1
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -2
Query: 700 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 560
V ++ LC + K E K+ V ES + KKF T L+P +R
Sbjct: 315 VAIVLALCSGLYNFKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYR 361
>At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1)
identical to P-glycoprotein GI:3849833 from [Arabidopsis
thaliana]; homologous to mammalian mdr gene,contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1286
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = +2
Query: 548 SSEVPPDGSQLRRYKLLAVRTKGFVIDRYFDLMVFGFLLRHDIAEQHQKANQPESLHLVV 727
S V DG +R+Y L A+R ++ + + +FG + +IA H+ A + E +
Sbjct: 1080 SGRVMIDGKDIRKYNLKAIRKHIAIVPQ--EPCLFGTTIYENIAYGHECATEAEIIQAAT 1137
Query: 728 LYQEKK 745
L K
Sbjct: 1138 LASAHK 1143
>At1g42470.1 68414.m04897 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (GB:AAB63982) (GI:2276463); contains Pfam
profile PF02460 Patched family
Length = 1272
Score = 29.5 bits (63), Expect = 2.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -2
Query: 700 VCFLMLLCYVVAKKKTEDHKVKIAVYYESLCPDSKKFITTQLAPVWR 560
V ++LLC + + K E K+ V S + K+F T LAP +R
Sbjct: 356 VSVVLLLCVGLIRFKVETRPDKLWVGSGSRAAEEKQFFDTHLAPFYR 402
>At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family
protein
Length = 846
Score = 29.5 bits (63), Expect = 2.7
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = -1
Query: 440 GASLRPEGPQPPGHGEDGDCNMPHEPDQPRQ 348
G S+ P GP PP H G P+ P +Q
Sbjct: 776 GYSIPPYGPPPPYHTPHGQAPQPYPPQAQQQ 806
>At5g43130.1 68418.m05265 transcription initiation factor IID
(TFIID) component TAF4 family protein weak similarity to
SP|O00268 Transcription initiation factor TFIID 135 kDa
subunit {Homo sapiens}; contains Pfam profile PF05236:
Transcription initiation factor TFIID component TAF4
family
Length = 712
Score = 29.1 bits (62), Expect = 3.6
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2
Query: 316 KMSESDKLKRCASSEQGDNLLASYGDKTD 230
K +E++KLK+ + SE+GD + S DK D
Sbjct: 556 KQAEAEKLKKPSESEEGDGGVDSEKDKED 584
>At4g03420.1 68417.m00469 expressed protein
Length = 310
Score = 28.7 bits (61), Expect = 4.8
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = -2
Query: 382 VICLMNQTSPDKSLDTCLTQVDKMSESDKLKRCASSE 272
+I L + + +S D+ SESDKL RCAS E
Sbjct: 97 LIRLRDDSEDGESRDSFSDSYSDESESDKLSRCASDE 133
>At1g06490.1 68414.m00688 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1933
Score = 28.7 bits (61), Expect = 4.8
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 7/68 (10%)
Frame = -2
Query: 682 LCYVVAKKKTEDHKVKIA-VY------YESLCPDSKKFITTQLAPVWRDFRGLVKVKMVP 524
LCY+ E H + VY YE+ PD + F+ + P+++ R +V+
Sbjct: 363 LCYIFHNMANEVHGILFGNVYPVTGDTYEAGAPDEEAFLRNVITPIYQVLR--KEVRRNK 420
Query: 523 YGKSTHDK 500
GK++H K
Sbjct: 421 NGKASHSK 428
>At3g59140.1 68416.m06593 ABC transporter family protein putative
multi resistance protein mrp - Arabidopsis thaliana,
EMBL:ATMRPPROT
Length = 1453
Score = 28.3 bits (60), Expect = 6.3
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = -2
Query: 406 QDTEKMEIVICLMNQTSPDKSLDTCLTQVDKMSESDKLKRCASSEQGDNLLASY 245
++T E V+ + N T P K ++ ++ K+ + +L + E+GD L Y
Sbjct: 827 RETAGSERVVAVENPTKPVKEINRVISSQSKVLKPSRLIKQEEREKGDTGLRPY 880
>At3g18290.1 68416.m02326 zinc finger protein-related weak alignment
to Pfam profiles: PF00097 Zinc finger, C3HC4 type (RING
finger) (2 copies)
Length = 1254
Score = 28.3 bits (60), Expect = 6.3
Identities = 13/44 (29%), Positives = 19/44 (43%)
Frame = +1
Query: 595 SCCQDKGIRNRPLF*PYGLRFSSSPRHSRAASESKPAGESSSCC 726
SCCQ + R ++P + A+ KP+G SCC
Sbjct: 563 SCCQHQDKRPAKRTAVLSCEKKTTPHSTEVANGCKPSGNGRSCC 606
>At2g37730.1 68415.m04627 fringe-related protein similarity to
predicted proteins + similar to hypothetical protein
GB:AAC23643 [Arabidopsis thaliana] + weak similarity to
Fringe [Schistocerca gregaria](GI:6573138);Fringe
encodes an extracellular protein that regulates Notch
signalling.
Length = 532
Score = 28.3 bits (60), Expect = 6.3
Identities = 17/48 (35%), Positives = 21/48 (43%)
Frame = +1
Query: 226 QHPFYHRTTRADCLLVHWMRIFSVYPTPTSCPLELSTCPKTCRGWSGS 369
QH F H TR + V W +YPT + EL T T + W S
Sbjct: 352 QHSFCHDQTRNWYVSVSWGYTIQIYPTLVTAK-ELETPFLTFKSWRTS 398
>At5g39440.1 68418.m04777 Snf1-related protein kinase, putative
similar to SNF1-related protein kinase KIN10 (EC
2.7.1.-) (AKIN10) [Arabidopsis thaliana]
SWISS-PROT:Q38997
Length = 494
Score = 27.9 bits (59), Expect = 8.4
Identities = 11/53 (20%), Positives = 26/53 (49%)
Frame = +2
Query: 602 VRTKGFVIDRYFDLMVFGFLLRHDIAEQHQKANQPESLHLVVLYQEKKRLYDF 760
++ G I ++ + FL+ I Q++ P +++V+ Y + L+D+
Sbjct: 55 IKNMGIEIKVQREIKILRFLMHPHIIRQYEVIETPNDIYVVMEYVKSGELFDY 107
>At5g38260.1 68418.m04612 serine/threonine protein kinase, putative
similar to receptor serine/threonine kinase PR55K
gi|1235680|gb|AAC49208; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 638
Score = 27.9 bits (59), Expect = 8.4
Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 5/86 (5%)
Frame = -2
Query: 598 KKFITTQLAPVWRDFRGLVKVKMVPYGK-----STHDKVDGKWSFICHHGADECYGNKVQ 434
K+ + L P + +GLV++K Y + GK F +G + C G KV
Sbjct: 288 KRRTSHHLRPRDNNLKGLVQLKQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVA 347
Query: 433 ACVLKDRNLQDTEKMEIVICLMNQTS 356
+LKD + E + M+QTS
Sbjct: 348 VKILKDFK-SNGEDFINEVASMSQTS 372
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,555,912
Number of Sequences: 28952
Number of extensions: 407183
Number of successful extensions: 1281
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1273
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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