Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_L17
(721 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 52 5e-07
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 51 7e-07
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 50 1e-06
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 50 2e-06
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 49 4e-06
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 47 1e-05
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 45 6e-05
At5g27770.1 68418.m03330 60S ribosomal protein L22 (RPL22C) ribo... 36 0.036
At3g05560.2 68416.m00614 60S ribosomal protein L22-2 (RPL22B) id... 35 0.047
At3g05560.1 68416.m00613 60S ribosomal protein L22-2 (RPL22B) id... 35 0.047
At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pf... 30 1.3
At1g73370.1 68414.m08492 sucrose synthase, putative / sucrose-UD... 29 2.3
At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative simila... 29 2.3
At5g52940.1 68418.m06569 hypothetical protein contains Pfam prof... 29 3.1
At1g03990.1 68414.m00385 alcohol oxidase-related low similarity ... 29 3.1
At1g02830.1 68414.m00243 60S ribosomal protein L22 (RPL22A) simi... 29 3.1
At5g23890.1 68418.m02806 expressed protein weak similarity to SP... 29 4.1
At3g20280.2 68416.m02570 PHD finger family protein contains Pfam... 29 4.1
At3g20280.1 68416.m02569 PHD finger family protein contains Pfam... 29 4.1
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 51.6 bits (118), Expect = 5e-07
Identities = 38/134 (28%), Positives = 58/134 (43%)
Frame = -3
Query: 446 PESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKKLKSQVVDL 267
P+S+G++ L S+NP + P + Y N+ DLD V++ H + ++ L
Sbjct: 344 PKSKGRLKLNSTNPRENPSVKFNYLENKADLD------ACLEMVLHLQHVARSETVTFFL 397
Query: 266 KVKQCRQWPFGSHEYWACYALNLASTQYHTVGTCAMGDKDIGVVDSRLRVHGVTGLRVVD 87
+ + G E + + T YH G C +G VVD +V+GV LRVVD
Sbjct: 398 GTQAHDKLVAGDEEL-KSFCIKNVRTYYHYHGGCVVG----SVVDEEYKVNGVKRLRVVD 452
Query: 86 ASVMPTITSGNTXA 45
S N A
Sbjct: 453 GSTFEESPGTNPMA 466
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 51.2 bits (117), Expect = 7e-07
Identities = 38/131 (29%), Positives = 57/131 (43%), Gaps = 9/131 (6%)
Frame = -3
Query: 446 PESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKKLKSQVVDL 267
P SRG + L+++NP+D P + YF + DL+ L VI+S + K K +
Sbjct: 412 PLSRGHLELRNTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYKYPLASA 471
Query: 266 K---------VKQCRQWPFGSHEYWACYALNLASTQYHTVGTCAMGDKDIGVVDSRLRVH 114
+ R S Y ++ T YH G C +G VVD+ +V
Sbjct: 472 RGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGK----VVDNNYKVL 527
Query: 113 GVTGLRVVDAS 81
GV LR++D S
Sbjct: 528 GVDALRIIDGS 538
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 50.4 bits (115), Expect = 1e-06
Identities = 49/183 (26%), Positives = 75/183 (40%), Gaps = 9/183 (4%)
Frame = -3
Query: 602 DYQVTATVSPTASFLPVLMCSQMLMLSDDICIALSEAGLGQETVFSLITLLHPESRGKIA 423
+ Q + T S T+ L + + + A + AGL L + P SRG +
Sbjct: 375 EMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGL------ILQKIAGPISRGHLE 428
Query: 422 LKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKKLK------SQVVDLKV 261
L+++NP+D P + Y+ DL + VINS F K K ++DL +
Sbjct: 429 LRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLML 488
Query: 260 K---QCRQWPFGSHEYWACYALNLASTQYHTVGTCAMGDKDIGVVDSRLRVHGVTGLRVV 90
R S + ++ T +H G C +G VVD RV G+ LRV+
Sbjct: 489 SVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVG----RVVDKNYRVLGIDSLRVI 544
Query: 89 DAS 81
D S
Sbjct: 545 DGS 547
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 50.0 bits (114), Expect = 2e-06
Identities = 38/131 (29%), Positives = 56/131 (42%), Gaps = 9/131 (6%)
Frame = -3
Query: 446 PESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKKLKSQVVDL 267
P S+G + L+++NP D P + Y+ DL+ L +INS F K K V
Sbjct: 437 PASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTA 496
Query: 266 K--VKQCRQWPFGSHEYWACYALNL-------ASTQYHTVGTCAMGDKDIGVVDSRLRVH 114
+ + P A NL ++ +H G C +G VVD +V
Sbjct: 497 RELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGK----VVDKNYKVL 552
Query: 113 GVTGLRVVDAS 81
G+ GLRV+D S
Sbjct: 553 GIDGLRVIDGS 563
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 48.8 bits (111), Expect = 4e-06
Identities = 37/134 (27%), Positives = 58/134 (43%)
Frame = -3
Query: 446 PESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKKLKSQVVDL 267
P+S+G++ L ++NP + P + Y N+ DLD V++ H + K+ L
Sbjct: 364 PKSKGRLKLNNTNPRENPSVTFNYLENKADLDACQ------EMVLHLQHVARSKTVTFFL 417
Query: 266 KVKQCRQWPFGSHEYWACYALNLASTQYHTVGTCAMGDKDIGVVDSRLRVHGVTGLRVVD 87
+ + G E + + T YH G C +G VV+ +V+GV LRVVD
Sbjct: 418 GTQAQDKLVAGDEEL-KKFCIKNVRTYYHYHGGCVVG----SVVNEEYKVNGVKRLRVVD 472
Query: 86 ASVMPTITSGNTXA 45
S N A
Sbjct: 473 GSTFEESPGTNPMA 486
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 46.8 bits (106), Expect = 1e-05
Identities = 40/145 (27%), Positives = 64/145 (44%), Gaps = 13/145 (8%)
Frame = -3
Query: 470 FSLITLLHPESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHFKK 291
F L ++ P S G + LK+ NP+D PI+ YF + +DL ++ V+ S F +
Sbjct: 401 FLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSR 460
Query: 290 LKSQVVD------------LKVKQCRQWPFGSHEYWA-CYALNLASTQYHTVGTCAMGDK 150
K V + ++ R P S A + + +T +H G C +G
Sbjct: 461 YKYADVSFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVG-- 518
Query: 149 DIGVVDSRLRVHGVTGLRVVDASVM 75
VVD +V G+ LRV+D S +
Sbjct: 519 --RVVDGDYKVIGIDRLRVIDMSTV 541
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 44.8 bits (101), Expect = 6e-05
Identities = 41/152 (26%), Positives = 63/152 (41%), Gaps = 10/152 (6%)
Frame = -3
Query: 470 FSLITLLHPESRGKIALKSSNPEDPPIIYSGYFTNENDLDNFAHYLENFNTVINSTHF-- 297
F L L +P SRG ++L ++N +D P + YF + DL + + V+ S F
Sbjct: 423 FILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLN 482
Query: 296 -----KKLKSQVVDLKVK---QCRQWPFGSHEYWACYALNLASTQYHTVGTCAMGDKDIG 141
K+ +++ L VK R + A + + T +H G C +G
Sbjct: 483 YTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGK---- 538
Query: 140 VVDSRLRVHGVTGLRVVDASVMPTITSGNTXA 45
VV +V GV LRV+D S N A
Sbjct: 539 VVSPNRKVLGVDRLRVIDGSTFDESPGTNPQA 570
>At5g27770.1 68418.m03330 60S ribosomal protein L22 (RPL22C)
ribosomal protein L22 (cytosolic), Rattus norvegicus,
PIR:S52084
Length = 124
Score = 35.5 bits (78), Expect = 0.036
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = +1
Query: 472 TVSCPKPASDRAMHISSLNMSICEHIST-GRNEAVGETVAVT 594
T+ C KP D+ M I+SL + E I G+ A+G++V++T
Sbjct: 19 TIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVSIT 60
>At3g05560.2 68416.m00614 60S ribosomal protein L22-2 (RPL22B)
identical to 60S ribosomal protein L22-2 SP:Q9M9W1 from
[Arabidopsis thaliana]
Length = 124
Score = 35.1 bits (77), Expect = 0.047
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
Frame = +1
Query: 472 TVSCPKPASDRAMHISSLNMSICEHIST-GRNEAVGETVAVT 594
T+ C KP D+ M I+SL + E I G+ A+G++V +T
Sbjct: 19 TIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVTIT 60
>At3g05560.1 68416.m00613 60S ribosomal protein L22-2 (RPL22B)
identical to 60S ribosomal protein L22-2 SP:Q9M9W1 from
[Arabidopsis thaliana]
Length = 124
Score = 35.1 bits (77), Expect = 0.047
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
Frame = +1
Query: 472 TVSCPKPASDRAMHISSLNMSICEHIST-GRNEAVGETVAVT 594
T+ C KP D+ M I+SL + E I G+ A+G++V +T
Sbjct: 19 TIDCSKPVDDKIMEIASLEKFLQERIKVGGKAGALGDSVTIT 60
>At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 654
Score = 30.3 bits (65), Expect = 1.3
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = -3
Query: 260 KQCRQWPFGSHEYWACYALN--LASTQYHTVGTCAMGDKDIGVVDSRLRVHGVTGLRVVD 87
K R W GS++ +A N + S Q++ V G +D ++R+ ++G VVD
Sbjct: 349 KTVRLWKVGSNDCLGVFAHNSYVTSVQFNPVNENYFMS---GSIDGKVRIWNISGCSVVD 405
Query: 86 ASVMPTITSGNTXAPGG 36
+ + I S P G
Sbjct: 406 WADLKDIISAVCYRPDG 422
>At1g73370.1 68414.m08492 sucrose synthase, putative / sucrose-UDP
glucosyltransferase, putative similar to sucrose
synthase GI:6682841 from [Citrus unshiu]
Length = 942
Score = 29.5 bits (63), Expect = 2.3
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = -3
Query: 554 VLMCSQMLMLSDDICIALSEAGLGQETVFSLITLLHPESRG 432
V + Q+ L +++ I +++ GLG + ++T L PE+RG
Sbjct: 310 VYILDQVRALEEELLIRINQQGLGFKPQILVVTRLIPEARG 350
>At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative similar
to p68 RNA helicase [Schizosaccharomyces pombe]
GI:173419
Length = 537
Score = 29.5 bits (63), Expect = 2.3
Identities = 14/30 (46%), Positives = 22/30 (73%), Gaps = 1/30 (3%)
Frame = +3
Query: 621 VQSDKAQNERXGTLSMFNKGSM-LLIAXDM 707
+ +KAQ+ER +LS+F +GS LL+A D+
Sbjct: 396 IHGNKAQSERTRSLSLFKEGSCPLLVATDV 425
>At5g52940.1 68418.m06569 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 362
Score = 29.1 bits (62), Expect = 3.1
Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 2/70 (2%)
Frame = -3
Query: 602 DYQVTATVSPTASFLPVLMCSQMLMLSDDICIALSEAGLGQETVFSLITLLHPESR--GK 429
D + T+ + ++ L ++ L DD+ + S+ + + L+TL H +++
Sbjct: 77 DGKARVTIGSSHGWVATLKDDGIVRLQDDLNLVASDTDPKRIPLPPLVTLPHCQTKVVTN 136
Query: 428 IALKSSNPED 399
+A+ SS+PED
Sbjct: 137 VAMSSSSPED 146
>At1g03990.1 68414.m00385 alcohol oxidase-related low similarity to
long chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]; Location
of EST 248L9T7, gb|AA713296
Length = 758
Score = 29.1 bits (62), Expect = 3.1
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = -3
Query: 182 HTVGTCAMGDKDI-GVVDSRLRVHGVTGLRVVDASVMPTITSGN 54
H +G C MG + G +D + L V DASV+PT N
Sbjct: 689 HQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPTALGVN 732
>At1g02830.1 68414.m00243 60S ribosomal protein L22 (RPL22A) similar
to ribosomal protein L22 GI:710294 from [Rattus
norvegicus]
Length = 127
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +1
Query: 475 VSCPKPASDRAMHISSLNMSICEHIST-GRNEAVGETVAVT 594
+ C KP D + I++L + E I G+ A+G +V++T
Sbjct: 22 IDCSKPVDDTILEIATLEKFLQERIKVRGKAGALGNSVSIT 62
>At5g23890.1 68418.m02806 expressed protein weak similarity to
SP|P12957 Caldesmon (CDM) {Gallus gallus}
Length = 946
Score = 28.7 bits (61), Expect = 4.1
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -3
Query: 455 LLHPESRGKIALKSSNPEDPPIIYSGYFTNENDLDN 348
LL ES + ++++N EDP + + TN +DL+N
Sbjct: 245 LLDAESSNLVGVENTNSEDPESLLNTEPTNVSDLEN 280
>At3g20280.2 68416.m02570 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 482
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 5/60 (8%)
Frame = +1
Query: 16 AAFSALIPPGAXVFPLVMVGITLASTTRRPVTP*-----TLNLESTTPISLSPMAQVPTV 180
A+ ++I G PL VG T++ST+ PV T N +TPI+ S +AQ PTV
Sbjct: 221 ASTISVINNGLISKPLTPVG-TMSSTSPLPVVNQLPVNATSNASPSTPITASLVAQAPTV 279
>At3g20280.1 68416.m02569 PHD finger family protein contains Pfam
profile: PF00628 PHD-finger
Length = 743
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 5/60 (8%)
Frame = +1
Query: 16 AAFSALIPPGAXVFPLVMVGITLASTTRRPVTP*-----TLNLESTTPISLSPMAQVPTV 180
A+ ++I G PL VG T++ST+ PV T N +TPI+ S +AQ PTV
Sbjct: 482 ASTISVINNGLISKPLTPVG-TMSSTSPLPVVNQLPVNATSNASPSTPITASLVAQAPTV 540
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,939,180
Number of Sequences: 28952
Number of extensions: 299226
Number of successful extensions: 743
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 718
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 740
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -