Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_L16
(754 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 138 5e-33
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 134 4e-32
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 128 5e-30
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 128 5e-30
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.067
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.82
At5g38990.1 68418.m04717 protein kinase family protein contains ... 30 1.9
At5g39000.1 68418.m04718 protein kinase family protein contains ... 29 4.4
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 4.4
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 29 4.4
At3g46390.1 68416.m05029 hypothetical protein 28 5.8
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 5.8
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 138 bits (333), Expect = 5e-33
Identities = 82/210 (39%), Positives = 113/210 (53%), Gaps = 11/210 (5%)
Frame = -2
Query: 678 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 505
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 504 ---ASYTSAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXD-----VDLFGSGXXXXXX 352
S SAE +G+SP+ + VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKK 122
Query: 351 XXXXXXXXRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 172
+A + K S K KSS+L+D+KPWDDETDMK++E VR+I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 171 ASKLVPVGYGINKLQIMCXIEDDKVAVDLL 82
ASKLVPVGYGI KL IMC I DD V++D +
Sbjct: 177 ASKLVPVGYGIKKLHIMCTIVDDLVSIDTM 206
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 134 bits (325), Expect = 4e-32
Identities = 80/210 (38%), Positives = 113/210 (53%), Gaps = 11/210 (5%)
Frame = -2
Query: 678 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 505
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 504 ---ASYTSAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXD-----VDLFGSGXXXXXX 352
S SAE +G++P+ + VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKK 122
Query: 351 XXXXXXXXRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 172
+A + K S K KSS+L+D+KPWDDETDMK++E V++I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 171 ASKLVPVGYGINKLQIMCXIEDDKVAVDLL 82
ASKLVPVGYGI KLQI+C I DD V++D +
Sbjct: 177 ASKLVPVGYGIKKLQILCTIVDDLVSIDTM 206
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 128 bits (308), Expect = 5e-30
Identities = 77/199 (38%), Positives = 102/199 (51%), Gaps = 3/199 (1%)
Frame = -2
Query: 669 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTS 490
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS +
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLA 66
Query: 489 AE---RKTWSQGTSPLXXXXXXXXXXXXXXXXXXXDVDLFGSGXXXXXXXXXXXXXXRLK 319
+ Q D+DLFG +
Sbjct: 67 KSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------R 120
Query: 318 AYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGI 139
A K +KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WGASKLVPVGYGI
Sbjct: 121 EAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGI 180
Query: 138 NKLQIMCXIEDDKVAVDLL 82
KL IM I DD V+ D L
Sbjct: 181 KKLTIMFTIVDDLVSPDNL 199
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 128 bits (308), Expect = 5e-30
Identities = 78/203 (38%), Positives = 105/203 (51%), Gaps = 7/203 (3%)
Frame = -2
Query: 669 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTS 490
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+ +
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLA 66
Query: 489 AERKTWSQGTS-------PLXXXXXXXXXXXXXXXXXXXDVDLFGSGXXXXXXXXXXXXX 331
+ G P D+DLF
Sbjct: 67 KSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE--- 123
Query: 330 XRLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPV 151
+ A K +KK KSS+LL+VKPWDDETDMK++E VR+++M GL WGASKLVPV
Sbjct: 124 ---REAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPV 180
Query: 150 GYGINKLQIMCXIEDDKVAVDLL 82
GYGI KL IM I DD V+VD L
Sbjct: 181 GYGIKKLTIMMTIVDDLVSVDNL 203
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.067
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = -2
Query: 648 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 505
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.82
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = +1
Query: 172 SPEKAFHFNSAYLVFHFLHIGFIIPW 249
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -3
Query: 536 FPTYYDGIIKLLHTLRLNAKHGLRAPAH*PPVLNPRLPPQ 417
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/56 (25%), Positives = 28/56 (50%)
Frame = -3
Query: 533 PTYYDGIIKLLHTLRLNAKHGLRAPAH*PPVLNPRLPPQQRKTTMTTTLIYLVLVT 366
P YYD I+ + L++N G A + P+++P L P + + +++ +T
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNRATPRIRKNKSHILPIT 445
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.7 bits (61), Expect = 4.4
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -2
Query: 303 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 172
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 28.7 bits (61), Expect = 4.4
Identities = 15/67 (22%), Positives = 28/67 (41%)
Frame = -2
Query: 282 IAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCXIEDD 103
+ S++L D+K WD+ M+ + ++ G W + + + + C ED
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDS 594
Query: 102 KVAVDLL 82
DLL
Sbjct: 595 GSLFDLL 601
>At3g46390.1 68416.m05029 hypothetical protein
Length = 69
Score = 28.3 bits (60), Expect = 5.8
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = -2
Query: 324 LKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGA--SKLVPV 151
+K Y+ K K+ + +I +D+K +M + E + I M G++W S L+ V
Sbjct: 1 MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60
Query: 150 GYGINKLQI 124
+ K QI
Sbjct: 61 DEALLKQQI 69
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = -2
Query: 648 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 553
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,772,629
Number of Sequences: 28952
Number of extensions: 292080
Number of successful extensions: 781
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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