Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_G16
(612 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g22756.1 68417.m03285 sterol desaturase family protein simila... 35 0.049
At4g22753.1 68417.m03284 sterol desaturase family protein simila... 29 1.8
At4g12110.1 68417.m01923 sterol desaturase family protein simila... 29 3.2
At4g10090.1 68417.m01651 expressed protein 28 4.2
At3g29270.2 68416.m03675 expressed protein 28 4.2
At3g29270.1 68416.m03674 expressed protein 28 4.2
At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR... 28 4.2
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.6
At1g55750.1 68414.m06383 transcription factor-related low simila... 27 7.4
At5g52280.1 68418.m06488 protein transport protein-related low s... 27 9.8
At5g45050.2 68418.m05524 disease resistance protein-related simi... 27 9.8
At5g45050.1 68418.m05523 disease resistance protein-related simi... 27 9.8
At5g18970.1 68418.m02253 AWPM-19-like membrane family protein co... 27 9.8
At4g10470.1 68417.m01719 expressed protein 27 9.8
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 27 9.8
>At4g22756.1 68417.m03285 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 299
Score = 34.7 bits (76), Expect = 0.049
Identities = 22/54 (40%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = -1
Query: 225 LYYLVICVWNY-SNRFSSLWKGYASPFHHY*KILHHYYCSLAGYANPKRHWDTV 67
+Y+LV NY +RF GY FHH +HH Y + GYA P HW V
Sbjct: 134 VYFLVEDYTNYWVHRFFHCKWGYEK-FHH----IHHEYTAPIGYAAPYAHWAEV 182
>At4g22753.1 68417.m03284 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 291
Score = 29.5 bits (63), Expect = 1.8
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 129 LHHYYCSLAGYANPKRHW 76
+HH Y S GYA+P HW
Sbjct: 158 IHHEYTSPIGYASPYAHW 175
>At4g12110.1 68417.m01923 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 298
Score = 28.7 bits (61), Expect = 3.2
Identities = 20/55 (36%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Frame = -1
Query: 225 LYYLVICVWNY--SNRFSSLWKGYASPFHHY*KILHHYYCSLAGYANPKRHWDTV 67
+Y+L+ NY F S W GY H +HH Y + GYA P HW V
Sbjct: 134 VYFLIEDYTNYWVHRFFHSKW-GY-DKIHR----VHHEYTAPIGYAAPYAHWAEV 182
>At4g10090.1 68417.m01651 expressed protein
Length = 262
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -1
Query: 231 YYLYYLVICVWNYSNRFSSLWKGYASPFHHY*KILHHYYCSLAGYANPKR 82
+ L+ L+ V + ++ + ++ +A PF HY +IL C+LA + + R
Sbjct: 41 FVLHQLMKRVLSSNSSDALIFLAFARPFSHYDRILRKLGCNLATHKSNNR 90
>At3g29270.2 68416.m03675 expressed protein
Length = 263
Score = 28.3 bits (60), Expect = 4.2
Identities = 29/94 (30%), Positives = 40/94 (42%)
Frame = -3
Query: 445 HFQGYPRELLPVTVQGIPSMHICLDFIPELLSQASLEKQIFAVDLVSHLSIQYALPKAMS 266
H + P V IP +I L+ L + F + ++ L I YA+P + +
Sbjct: 169 HHRSQPEPSRSVNDHRIPRDNIQTSLRKSLVFFVQLTAK-FPLVVIFLLIILYAIPTSAA 227
Query: 265 IARLCVNTLSTLLSVLPSDLRLELFQPVLKSLER 164
I L + L TLL LPS L L P L L R
Sbjct: 228 I--LAMYILVTLLLALPSFLILYFAYPCLDWLVR 259
>At3g29270.1 68416.m03674 expressed protein
Length = 263
Score = 28.3 bits (60), Expect = 4.2
Identities = 29/94 (30%), Positives = 40/94 (42%)
Frame = -3
Query: 445 HFQGYPRELLPVTVQGIPSMHICLDFIPELLSQASLEKQIFAVDLVSHLSIQYALPKAMS 266
H + P V IP +I L+ L + F + ++ L I YA+P + +
Sbjct: 169 HHRSQPEPSRSVNDHRIPRDNIQTSLRKSLVFFVQLTAK-FPLVVIFLLIILYAIPTSAA 227
Query: 265 IARLCVNTLSTLLSVLPSDLRLELFQPVLKSLER 164
I L + L TLL LPS L L P L L R
Sbjct: 228 I--LAMYILVTLLLALPSFLILYFAYPCLDWLVR 259
>At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 897
Score = 28.3 bits (60), Expect = 4.2
Identities = 29/126 (23%), Positives = 58/126 (46%), Gaps = 1/126 (0%)
Frame = -3
Query: 379 CLDFIPELLSQASLEKQIFAVDLVSHLSIQYALPKAMSIARLCVNTLSTLLSVLPSDLRL 200
CL +P+L A+LE+ + + + + I ++ I L + + +L V+P+ + L
Sbjct: 637 CLKELPDLSKAANLER-LDVAECNALVEIPSSVANLHKIVNLHMESCESL-EVIPTLINL 694
Query: 199 ELFQPV-LKSLERICIAFPSLLEDITSLLLQLGRICESQASLGHCWNDTPILGETAYVDS 23
+ + + R+ +FP + + L+++ + E AS HC T T Y+ S
Sbjct: 695 ASLKIINIHDCPRLK-SFPDVPTSLEELVIEKTGVQELPASFRHCTGVT-----TLYICS 748
Query: 22 ERQPET 5
R +T
Sbjct: 749 NRNLKT 754
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = -3
Query: 397 IPSMHICLDFIPELLSQASLEKQIFAVDLVSHLSIQYALPKAMSIARLCV 248
+PSM + + +P L S SL +V S YAL K +S++R V
Sbjct: 1 MPSMLVLVGTMPSLASLVSLGGACASVSGTSSSDASYALVKRVSLSRRSV 50
>At1g55750.1 68414.m06383 transcription factor-related low
similarity to SP|Q9DBA9 TFIIH basal transcription factor
complex p62 subunit {Mus musculus}; contains Pfam
profile PF03909: BSD domain
Length = 591
Score = 27.5 bits (58), Expect = 7.4
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -3
Query: 403 QGIPSMHICLDFIPELLSQASLEKQIFAVDLVS-HLSIQ 290
+ P MH C DFI + L++ LE V S LSI+
Sbjct: 83 ENYPDMHACRDFITKALAKCELEPNKSVVSTSSEQLSIK 121
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 27.1 bits (57), Expect = 9.8
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 559 PDDQXKPELMWSLREVRSIICSFLHQIFIS 470
PDD KP EV+ ICS+ + I++S
Sbjct: 39 PDDVGKPTFKLEKSEVKEGICSWENPIYVS 68
>At5g45050.2 68418.m05524 disease resistance protein-related similar
to NL27 [Solanum tuberosum] GI:3947735; contains Pfam
profiles PF03106: WRKY DNA -binding domain, PF00931:
NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1344
Score = 27.1 bits (57), Expect = 9.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = -3
Query: 496 SFLHQIFISEPSLAKLVHFQGYPRELLPVTVQGIPSMHICLDF 368
+FL S P++ +L+H++ YP + LP I + I + +
Sbjct: 536 NFLKGSLSSLPNVLRLLHWENYPLQFLPQNFDPIHLVEINMPY 578
>At5g45050.1 68418.m05523 disease resistance protein-related similar
to NL27 [Solanum tuberosum] GI:3947735; contains Pfam
profiles PF03106: WRKY DNA -binding domain, PF00931:
NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1372
Score = 27.1 bits (57), Expect = 9.8
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = -3
Query: 496 SFLHQIFISEPSLAKLVHFQGYPRELLPVTVQGIPSMHICLDF 368
+FL S P++ +L+H++ YP + LP I + I + +
Sbjct: 536 NFLKGSLSSLPNVLRLLHWENYPLQFLPQNFDPIHLVEINMPY 578
>At5g18970.1 68418.m02253 AWPM-19-like membrane family protein
contains Pfam profile: PF05512 AWPM-19-like family
Length = 171
Score = 27.1 bits (57), Expect = 9.8
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +3
Query: 87 WDSHILPSCNSNDVISSSNDGKAMHILSRDLRTGWNNSRRKSL 215
WDS L S ++ +IS S AM + +++ GW + ++L
Sbjct: 92 WDSPNLHSAAASSLISWSLTLLAMGLACKEINIGWTEANLRTL 134
>At4g10470.1 68417.m01719 expressed protein
Length = 388
Score = 27.1 bits (57), Expect = 9.8
Identities = 16/52 (30%), Positives = 24/52 (46%)
Frame = +3
Query: 135 SSNDGKAMHILSRDLRTGWNNSRRKSLGNTDNSVERVFTHSLAIDIALGKAY 290
S G A H+L+ + G + S N+ + V THSL +D +L Y
Sbjct: 156 SEKHGAADHMLTMETNVGSTSGVSSSDSMLLNTFKVVGTHSLVVDRSLRNRY 207
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 27.1 bits (57), Expect = 9.8
Identities = 22/59 (37%), Positives = 28/59 (47%)
Frame = -3
Query: 247 NTLSTLLSVLPSDLRLELFQPVLKSLERICIAFPSLLEDITSLLLQLGRICESQASLGH 71
+ L T L SDL L+ F K LERI P LE TS+ L R+ + + GH
Sbjct: 28 SALETTPGSLLSDLWLDRFPDPFKILERI----PLGLERDTSVALSPARVDWKETAEGH 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,364,645
Number of Sequences: 28952
Number of extensions: 286776
Number of successful extensions: 760
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 733
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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