Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_F24
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 113 8e-26
At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 113 8e-26
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 113 8e-26
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 30 1.6
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 29 2.9
At3g08670.1 68416.m01007 expressed protein 29 3.8
At1g20540.1 68414.m02559 transducin family protein / WD-40 repea... 29 3.8
At3g05050.1 68416.m00548 protein kinase family protein contains ... 28 5.0
At2g27270.1 68415.m03277 expressed protein 28 5.0
At1g27190.1 68414.m03312 leucine-rich repeat transmembrane prote... 27 8.8
At1g03060.1 68414.m00280 WD-40 repeat family protein / beige-rel... 27 8.8
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 113 bits (273), Expect = 8e-26
Identities = 70/167 (41%), Positives = 95/167 (56%)
Frame = -2
Query: 575 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 396
P+++ FYP ++ ++ R +K + +R ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPPKFYPAED-VKKPLPNRRTAKPAK-LRASITPGTVLIILAGRFKGKRVVFLKQLA 114
Query: 395 SGLLLVTGPFAFNSCPLRRIPQRYVICTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXX 216
SGLLLVTGPF N PLRR+ Q YVI TST++ + L K F+D YF
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYF---GKVAEKKKK 170
Query: 215 XKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRG 75
EG+ A K+EK + +K DQK VD A+IKAI A P+ K G
Sbjct: 171 KTEGEFFEAEKEEKKEIPQGKKDDQKAVDAALIKAIEAVPELKTYLG 217
>At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 113 bits (273), Expect = 8e-26
Identities = 70/167 (41%), Positives = 95/167 (56%)
Frame = -2
Query: 575 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 396
P+++ FYP ++ ++ R +K + +R ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPPKFYPAED-VKKPLPNRRTAKPTK-LRASITPGTVLIILAGRFKGKRVVFLKQLA 114
Query: 395 SGLLLVTGPFAFNSCPLRRIPQRYVICTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXX 216
SGLLLVTGPF N PLRR+ Q YVI TST++ + L K F+D YF
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQAYVIGTSTKVDISGVTLDK-FDDKYF---GKVAEKKKK 170
Query: 215 XKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRG 75
EG+ A K+EK + +K DQK VD A+IKAI A P+ K G
Sbjct: 171 KTEGEFFEAEKEEKKEIPQVKKDDQKAVDAALIKAIEAVPELKTYLG 217
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 113 bits (273), Expect = 8e-26
Identities = 70/167 (41%), Positives = 96/167 (57%)
Frame = -2
Query: 575 PLKRRKSFYPTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILP 396
P+++ FYP ++ ++ R K + ++ ++ GTV I+LAGR GKRVV + L
Sbjct: 57 PVEKPAKFYPAED-VKKPLVNRRKPKPTK-LKASITPGTVLIILAGRFKGKRVVFLKQLS 114
Query: 395 SGLLLVTGPFAFNSCPLRRIPQRYVICTSTRISLGNFKLPKHFNDDYFXXXXXXXXXXXX 216
SGLLLVTGPF N PLRR+ Q YVI TST+I + K F+D YF
Sbjct: 115 SGLLLVTGPFKINGVPLRRVNQAYVIGTSTKIDISGVNTEK-FDDKYF---GKVAEKKKK 170
Query: 215 XKEGDDIFATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKVLRG 75
EG+ A K+EK +++K DQKTVD A+IK+I A P+ KV G
Sbjct: 171 KTEGEFFEAEKEEKKEIPQEKKEDQKTVDAALIKSIEAVPELKVYLG 217
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = -2
Query: 575 PLKRRKSFYPTQEKIRASSGGRPFS-KHVRR 486
P++RR+S P +E++ S GGR S H+++
Sbjct: 525 PVRRRRSLTPDEERVSLSQGGRHTSPSHIKQ 555
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis
thaliana}
Length = 1473
Score = 29.1 bits (62), Expect = 2.9
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = -2
Query: 191 ATKKEKYVPSEQRKTDQKTVDEAVIKAIGARPDKKV 84
ATK K P+ RK ++TV + AIG P+KKV
Sbjct: 1354 ATKAAK-PPAAPRKRGKQTVASTEVLAIGVSPEKKV 1388
>At3g08670.1 68416.m01007 expressed protein
Length = 567
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/56 (33%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +1
Query: 46 SGVRRPPXRYPR-STFLSGRAPMALITASSTVF*SVLRCSDGTYFSFFVAKMSSPS 210
S V RP + S+F SGR+P +++ SS S +R S + S A+ S+P+
Sbjct: 155 SSVTRPSISTSQYSSFTSGRSPSSILNTSSASVSSYIRPSSPSSRSSSSARPSTPT 210
>At1g20540.1 68414.m02559 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
similar to Rbap46 polypeptide (GI:9454362) [Gallus
gallus]
Length = 351
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 2/36 (5%)
Frame = -1
Query: 609 WR*EEWGNQNSTPQT*EV--LLPHSGENPCLIWWPS 508
W+ E Q ++PQ V L H G+ C++WWPS
Sbjct: 96 WQIPELYGQLNSPQLERVASLDAHVGKINCVLWWPS 131
>At3g05050.1 68416.m00548 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 593
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/67 (32%), Positives = 34/67 (50%)
Frame = -3
Query: 289 TSNCQNTSMMITSRRIRSASNVQSNAKRVMTSLPQKKRNTFHLSSAKPIRRQSTRL*SKP 110
T ++TS ++ + SASN + + + QKK N F ++ AK + + R SKP
Sbjct: 40 TETTESTSAVV----VASASNGEE-VRNHEDVVDQKKENGFVVTEAKERKSKGERKRSKP 94
Query: 109 SEPDPTR 89
PDP R
Sbjct: 95 --PDPRR 99
>At2g27270.1 68415.m03277 expressed protein
Length = 231
Score = 28.3 bits (60), Expect = 5.0
Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = -2
Query: 548 PTQEKIRASSGGRPFSKHVRRIRPNLKIGTVCILLAGRHAGKRVVLVGILPSGLLLVTGP 369
PT++K A G SKH R + + + C+ GK ++ G+ + +LL+
Sbjct: 21 PTKKKTEAKEEGAAASKHSRVVYSD---KSRCL----SKNGKTIIYFGVPAALILLIICV 73
Query: 368 FAFNSCPLR-RIPQ 330
FAFN ++ R+P+
Sbjct: 74 FAFNYIAIQPRVPR 87
>At1g27190.1 68414.m03312 leucine-rich repeat transmembrane protein
kinase, putative similar to CLV1 receptor kinase
GB:AAB58929 GI:2160756 from [Arabidopsis thaliana]
Length = 601
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/37 (29%), Positives = 15/37 (40%)
Frame = -1
Query: 432 CRQEGCTCWNSAQRSAFSYWTFCIQFVPATPHSSALC 322
C+ G +CWN + S +Q P S LC
Sbjct: 59 CKLTGVSCWNEKENRIISLQLQSMQLAGEIPESLKLC 95
>At1g03060.1 68414.m00280 WD-40 repeat family protein / beige-related
similar to BEIGE (GI:3928547) [Rattus norvegicus];
Similar to gb|U70015 lysosomal trafficking regulator from
Mus musculus and contains 2 Pfam PF00400 WD-40, G-beta
repeats. ESTs gb|T43386 and gb|AA395236 come from this
gene
Length = 3601
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/63 (26%), Positives = 28/63 (44%)
Frame = -3
Query: 319 SAPPPEFHSATSNCQNTSMMITSRRIRSASNVQSNAKRVMTSLPQKKRNTFHLSSAKPIR 140
S PP +S Q + + T + AS ++K MTS Q ++N F + S +
Sbjct: 1082 SWPPAAGYSFVCWFQFRNFLTTQGKESEASKAGGSSKTRMTSAQQHEQNIFRMFSVGAVS 1141
Query: 139 RQS 131
+S
Sbjct: 1142 NES 1144
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,132,365
Number of Sequences: 28952
Number of extensions: 334716
Number of successful extensions: 924
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 892
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 921
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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