Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_F23
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 247 7e-66
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 247 7e-66
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 247 7e-66
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 247 7e-66
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 141 5e-34
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 80 1e-15
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 62 4e-10
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 44 1e-04
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 38 0.005
At4g27900.2 68417.m04005 expressed protein 32 0.46
At4g27900.1 68417.m04004 expressed protein 32 0.46
At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /... 29 3.3
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 5.7
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ... 28 7.6
At5g07670.1 68418.m00878 F-box family protein similar to unknown... 27 10.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 247 bits (604), Expect = 7e-66
Identities = 110/155 (70%), Positives = 136/155 (87%)
Frame = -2
Query: 742 EVKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 563
EVKSVEMHHE+L EA+PGDNVGFNVK V+VK+L+RGYVA +SK++P KGAA+FT+QVI++
Sbjct: 276 EVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM 335
Query: 562 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 383
NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+
Sbjct: 336 NHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPT 395
Query: 382 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 278
KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 396 KPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 247 bits (604), Expect = 7e-66
Identities = 110/155 (70%), Positives = 136/155 (87%)
Frame = -2
Query: 742 EVKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 563
EVKSVEMHHE+L EA+PGDNVGFNVK V+VK+L+RGYVA +SK++P KGAA+FT+QVI++
Sbjct: 276 EVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM 335
Query: 562 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 383
NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+
Sbjct: 336 NHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPT 395
Query: 382 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 278
KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 396 KPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 247 bits (604), Expect = 7e-66
Identities = 110/155 (70%), Positives = 136/155 (87%)
Frame = -2
Query: 742 EVKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 563
EVKSVEMHHE+L EA+PGDNVGFNVK V+VK+L+RGYVA +SK++P KGAA+FT+QVI++
Sbjct: 276 EVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM 335
Query: 562 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 383
NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+
Sbjct: 336 NHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPT 395
Query: 382 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 278
KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 396 KPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 247 bits (604), Expect = 7e-66
Identities = 110/155 (70%), Positives = 136/155 (87%)
Frame = -2
Query: 742 EVKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 563
EVKSVEMHHE+L EA+PGDNVGFNVK V+VK+L+RGYVA +SK++P KGAA+FT+QVI++
Sbjct: 276 EVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM 335
Query: 562 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPS 383
NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+
Sbjct: 336 NHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPT 395
Query: 382 KPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 278
KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 396 KPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 141 bits (341), Expect = 5e-34
Identities = 61/95 (64%), Positives = 77/95 (81%)
Frame = -2
Query: 565 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 386
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTP 60
Query: 385 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 281
+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 61 TKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 80.2 bits (189), Expect = 1e-15
Identities = 41/153 (26%), Positives = 78/153 (50%), Gaps = 2/153 (1%)
Frame = -2
Query: 742 EVKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 563
+V ++ + ++ A PG+N+ + + +++ G+V S NP +F AQ+ +L
Sbjct: 371 KVVAIYCDEDKVKRAGPGENLRVRITGIEDEDILSGFVLS-SIVNPVPAVTEFVAQLQIL 429
Query: 562 N--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLV 389
+ GY +L H C+ E+K ++D +T K + +K+G A + +
Sbjct: 430 ELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQ 489
Query: 388 PSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 290
+ +C+E F +FP LGRF +R +T+AVG +
Sbjct: 490 VTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 62.1 bits (144), Expect = 4e-10
Identities = 40/153 (26%), Positives = 72/153 (47%)
Frame = -2
Query: 739 VKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 560
++S+E +A A GDNV ++ + ++ G V + P A V+VL
Sbjct: 512 IRSLERDSQACTIARAGDNVALALQGIDANQVMAGDVLCHP-DFPVSVATHLELMVLVLE 570
Query: 559 HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSK 380
I G H A A ++ +D +TG+ T+ +P+ + + +A++ +
Sbjct: 571 GATPILLGSQLEFHVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQN 630
Query: 379 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 281
P+CVE+F E LGR +R +TVA+G + +
Sbjct: 631 PVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 43.6 bits (98), Expect = 1e-04
Identities = 40/156 (25%), Positives = 63/156 (40%), Gaps = 3/156 (1%)
Frame = -2
Query: 739 VKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 560
V VEM + L EA+ GDNVG ++ + +++RG V + P + V+
Sbjct: 333 VTGVEMFQKILDEALAGDNVGLLLRGIQKADIQRGMVLAKPGSITPHTKFEAIIYVLKKE 392
Query: 559 HPGQIS---NGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLV 389
G+ S GY P T + K +I D + K + GD + +
Sbjct: 393 EGGRHSPFFAGYRPQFYMRTTDVTGKVTKIMNDKDEES--------KMVMPGDRVKIVVE 444
Query: 388 PSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 281
P+ E RFA+R+ +TV GVI +
Sbjct: 445 LIVPVACEQGM------RFAIREGGKTVGAGVIGTI 474
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 38.3 bits (85), Expect = 0.005
Identities = 43/155 (27%), Positives = 67/155 (43%), Gaps = 2/155 (1%)
Frame = -2
Query: 739 VKSVEMHHEALQEAVPGDNVGFNVKXVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 560
V VEM + L GDNVG ++ + ++++RG V +K K F A++ VL
Sbjct: 316 VTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVI--AKPGSCKTYKKFEAEIYVLT 373
Query: 559 HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGD--AAIVNLVP 386
+T + + A+I KV+ N K + GD A+ L+
Sbjct: 374 K--DEGGRHTAFFSNYRPQFYLRTADITGKVE------LPENVKMVMPGDNVTAVFELIM 425
Query: 385 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 281
PL E+ Q RFA+R+ +TV GV+ V
Sbjct: 426 PVPL--ETGQ------RFALREGGRTVGAGVVSKV 452
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.46
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 433 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 341
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.46
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 433 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 341
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Salix gilgiana] GI:6714524; contains
PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 414
Score = 29.1 bits (62), Expect = 3.3
Identities = 21/71 (29%), Positives = 33/71 (46%)
Frame = +2
Query: 431 WVDFSRFTSTTVNFFFDFCKFAGNVGSVAIQDWCVTV*DLTRMVKHNDLSCKICSTLRWV 610
W + +S++VN +F+ VGS+ C V +LT VK + K S W+
Sbjct: 58 WDKACKSSSSSVNLIIPRGEFS--VGSLRFSGPCTNVSNLTVRVKASTDLSKYRSGGGWI 115
Query: 611 VFGVTSNITTT 643
FG + +T T
Sbjct: 116 QFGWINGLTLT 126
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 5.7
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -2
Query: 493 KFAEIKEKVDRRTGKSTEVNPKSI 422
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 578
Score = 27.9 bits (59), Expect = 7.6
Identities = 19/67 (28%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +3
Query: 126 VCSPFFLRNTFR*MKYRS-CMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPT 302
+C L + + +K R C+ A N + F+ F A L+TLPPP+ P+
Sbjct: 453 LCQWLVLSDMYGVLKIREYCLDLVACNFEA-FVETHEFRAMLLTLPPPSGDSSLRTTVPS 511
Query: 303 ATVCLMS 323
A +M+
Sbjct: 512 APGAMMT 518
>At5g07670.1 68418.m00878 F-box family protein similar to unknown
protein (pir||C71419) ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 476
Score = 27.5 bits (58), Expect = 10.0
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 6/58 (10%)
Frame = +2
Query: 584 KICSTLRWVVF----GVTSNITTTQFLDGHV--LYVETYIVSRYSFLESFVVHLHRLD 739
K+C R +VF G+ ++I + G V LY+E + S LES ++H H L+
Sbjct: 360 KMCEAAREIVFQDCWGLDNDIFSLAMAFGRVKLLYLEGCSLLTTSGLESVILHWHELE 417
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,928,424
Number of Sequences: 28952
Number of extensions: 328872
Number of successful extensions: 934
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 931
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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