BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_pT_F09 (729 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC167.02 |ptb1||geranylgeranyltransferase II beta subunit |Sch... 264 1e-71 SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni... 110 2e-25 SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit Cpp... 104 1e-23 SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 32 0.073 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 30 0.29 SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 27 2.1 SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc... 27 2.1 SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase ... 27 3.6 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 25 8.4 >SPAC167.02 |ptb1||geranylgeranyltransferase II beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 311 Score = 264 bits (646), Expect = 1e-71 Identities = 110/225 (48%), Positives = 156/225 (69%) Frame = -1 Query: 726 AVQVXAMYDRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDXRFSFCAVMCLSLLQRLD 547 AVQV AM D L VD + + YI+ LQ EDGS GD+WGEID RF + + CL++L +LD Sbjct: 87 AVQVLAMLDSLHVVDKDKVASYIIGLQNEDGSMKGDRWGEIDARFLYSGINCLAILGKLD 146 Query: 546 AIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALHADELAWWLC 367 ++ N V++++ C NFDGGFG PG+ESH +++ CV L I ++D + + L WW+ Sbjct: 147 YLNKNTAVDWLMKCYNFDGGFGLCPGAESHGAMVFTCVAALKILNKLDLIDEELLGWWIS 206 Query: 366 ERQLPSGGLNGRPEKLPDLCYSWWVMSSLSMLNRIHWVDKKSLEEFILACQDYETGGFSD 187 ERQ+ GGLNGRPEKLPD CY WW +S L+++ ++ W+D+ L +F+L QD ++GGF+D Sbjct: 207 ERQVKGGGLNGRPEKLPDSCYGWWDLSPLAIIGKLDWIDRNQLIDFLLGTQDADSGGFAD 266 Query: 186 RPGDITDPFHTLFGLAGLSLLGNTSIKPVNPTYCMPQETIDRLKL 52 R D TD +HT F LAGLSLL +I+PV+P +C+P E ++KL Sbjct: 267 RKEDATDVYHTCFSLAGLSLLQFPNIEPVDPRFCLPLEVTQKMKL 311 Score = 62.1 bits (144), Expect = 8e-11 Identities = 35/134 (26%), Positives = 70/134 (52%), Gaps = 2/134 (1%) Frame = -1 Query: 573 CLS--LLQRLDAIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDA 400 C+S LL++ D ID + V F+LSC+ GGF PG + H V L++ + Sbjct: 40 CMSFWLLKKKDQIDKERIVSFLLSCLTESGGFACYPGHDDHITNTVYAVQVLAMLDSLHV 99 Query: 399 LHADELAWWLCERQLPSGGLNGRPEKLPDLCYSWWVMSSLSMLNRIHWVDKKSLEEFILA 220 + D++A ++ Q G + G D + + ++ L++L ++ +++K + ++++ Sbjct: 100 VDKDKVASYIIGLQNEDGSMKGDRWGEIDARFLYSGINCLAILGKLDYLNKNTAVDWLMK 159 Query: 219 CQDYETGGFSDRPG 178 C +++ GGF PG Sbjct: 160 CYNFD-GGFGLCPG 172 Score = 40.7 bits (91), Expect = 2e-04 Identities = 35/154 (22%), Positives = 65/154 (42%), Gaps = 1/154 (0%) Frame = -1 Query: 729 SAVQVXAMYDRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDXRFSFCAVMCLSLLQRL 550 S + A+ +LD ++ V +++ DG F E F V L +L +L Sbjct: 134 SGINCLAILGKLDYLNKNTAVDWLMKCYNFDGGFGLCPGAESHGAMVFTCVAALKILNKL 193 Query: 549 DAIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALHADELAWWL 370 D ID ++ GG +P + + + L+I ++D + ++L +L Sbjct: 194 DLIDEELLGWWISERQVKGGGLNGRPEKLPDSCYGWWDLSPLAIIGKLDWIDRNQLIDFL 253 Query: 369 CERQ-LPSGGLNGRPEKLPDLCYSWWVMSSLSML 271 Q SGG R E D+ ++ + ++ LS+L Sbjct: 254 LGTQDADSGGFADRKEDATDVYHTCFSLAGLSLL 287 Score = 40.3 bits (90), Expect = 3e-04 Identities = 28/113 (24%), Positives = 51/113 (45%) Frame = -1 Query: 459 HAGLIYCCVGTLSICKRMDALHADELAWWLCERQLPSGGLNGRPEKLPDLCYSWWVMSSL 280 H IY + + K+ D + + + +L SGG P + + + + L Sbjct: 32 HVSAIYWSCMSFWLLKKKDQIDKERIVSFLLSCLTESGGFACYPGHDDHITNTVYAVQVL 91 Query: 279 SMLNRIHWVDKKSLEEFILACQDYETGGFSDRPGDITDPFHTLFGLAGLSLLG 121 +ML+ +H VDK + +I+ Q+ + DR G+I D G+ L++LG Sbjct: 92 AMLDSLHVVDKDKVASYIIGLQNEDGSMKGDRWGEI-DARFLYSGINCLAILG 143 Score = 34.3 bits (75), Expect = 0.018 Identities = 17/64 (26%), Positives = 35/64 (54%) Frame = -1 Query: 300 WWVMSSLSMLNRIHWVDKKSLEEFILACQDYETGGFSDRPGDITDPFHTLFGLAGLSLLG 121 +W S +L + +DK+ + F+L+C E+GGF+ PG +T++ + L++L Sbjct: 37 YWSCMSFWLLKKKDQIDKERIVSFLLSCLT-ESGGFACYPGHDDHITNTVYAVQVLAMLD 95 Query: 120 NTSI 109 + + Sbjct: 96 SLHV 99 >SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit Cwg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 110 bits (264), Expect = 2e-25 Identities = 69/233 (29%), Positives = 110/233 (47%), Gaps = 20/233 (8%) Frame = -1 Query: 702 DRLDAVDIEGIVRYIVSLQQEDGSFYG---DKWGEIDXRFSFCAVMCLSLLQRLDAIDIN 532 D L +D + I ++ + G F + D R + A SLL D Sbjct: 112 DNLSRIDRDLIKNFVELCKTSQGHFRSIAVPSCSDQDMRQLYMATTIASLLD-FSLSDPL 170 Query: 531 KTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRM----------------DA 400 +++++ SC ++GGF P E+HAG +C + + S+ +M D Sbjct: 171 CSIQYIKSCQRYEGGFSLLPYGEAHAGATFCALASWSLILKMIPNSSLNTSNQSYNLMDC 230 Query: 399 L-HADELAWWLCERQLPSGGLNGRPEKLPDLCYSWWVMSSLSMLNRIHWVDKKSLEEFIL 223 + + L WL RQL SGGLNGR K D CY++WV+SSL +L+ + ++D LE+++L Sbjct: 231 VPKVERLIRWLASRQLSSGGLNGRTNKDVDTCYAYWVLSSLKLLDALPFIDGGELEKYLL 290 Query: 222 ACQDYETGGFSDRPGDITDPFHTLFGLAGLSLLGNTSIKPVNPTYCMPQETID 64 + GGFS PG+ D H+ GL ++ + S VN M + I+ Sbjct: 291 LHAQHALGGFSKTPGEFPDVLHSALGLYAMAYQDDKSFPKVNADIHMTSKYIN 343 >SPAC17G6.04c |cpp1||protein farnesyltransferase beta subunit Cpp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 382 Score = 104 bits (249), Expect = 1e-23 Identities = 63/205 (30%), Positives = 106/205 (51%), Gaps = 14/205 (6%) Frame = -1 Query: 702 DRLDAVDIEGIVRYIVSLQQEDGSFYGDKWGEIDXRFSFCAVMCLSLLQRLDAID--INK 529 D ++ + + ++ SL+ DGSF + GE D R + AV C+S L + D Sbjct: 122 DAYSLIERDRLYDWLFSLKNPDGSFRVNNEGESDARSVYAAV-CVSSLVGISMDDPLFEG 180 Query: 528 TVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDALHADELAWWLCERQLPS 349 T++++ C ++GG P +E+H G +C + +++ +D L+ +L+ WL +RQ P+ Sbjct: 181 TLQWLCKCQTYEGGLSGVPYAEAHGGYTFCALAAIALLGGLDNLNEIKLSTWLVQRQDPA 240 Query: 348 -GGLNGRPEKLPDLCYSWWVMSSLSML-----NRIH------WVDKKSLEEFILACQDYE 205 G +GR KL D CYSWWV +S ++ + H + + + L +IL C Sbjct: 241 LYGFSGRSNKLVDGCYSWWVGASHVIVASGYGSASHKSLPNLFYNPEKLLGYILQCCQST 300 Query: 204 TGGFSDRPGDITDPFHTLFGLAGLS 130 +GG D+P D +HT + L GLS Sbjct: 301 SGGLRDKPPKRPDQYHTCYCLLGLS 325 Score = 46.0 bits (104), Expect = 6e-06 Identities = 41/155 (26%), Positives = 68/155 (43%), Gaps = 5/155 (3%) Frame = -1 Query: 570 LSLLQRLDAIDINKTVEFVLSCMNFDGGFGSKPGSESHAGLIYCCVGTLSICKRMDA--- 400 L++L +LD+ + + V GGF G + H Y + ++ +C DA Sbjct: 67 LAILGKLDSSVCERAISSVRQLKGPSGGFCGGNGQDEHLLSTYASILSICLCDSTDAYSL 126 Query: 399 LHADELAWWLCERQLPSGG--LNGRPEKLPDLCYSWWVMSSLSMLNRIHWVDKKSLEEFI 226 + D L WL + P G +N E Y+ +SSL ++ + + +L +++ Sbjct: 127 IERDRLYDWLFSLKNPDGSFRVNNEGESDARSVYAAVCVSSLVGISMDDPLFEGTL-QWL 185 Query: 225 LACQDYETGGFSDRPGDITDPFHTLFGLAGLSLLG 121 CQ YE GG S P +T LA ++LLG Sbjct: 186 CKCQTYE-GGLSGVPYAEAHGGYTFCALAAIALLG 219 >SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 32.3 bits (70), Expect = 0.073 Identities = 28/91 (30%), Positives = 42/91 (46%), Gaps = 10/91 (10%) Frame = -1 Query: 726 AVQVXAMYD---RLDAVD--IEGIVRYIVSLQQEDGSFYGDKWGEIDXRFSFCAVMCLSL 562 A++ YD R D ++ IE + Y+V +Q+ DGS+YG W F++ A+ Sbjct: 535 ALRAFTKYDPGYRRDEIENTIENALEYVVKMQRPDGSWYG-SWA---ICFTYAAMFATGS 590 Query: 561 LQRLDAIDIN-----KTVEFVLSCMNFDGGF 484 L N K EF+LS DGG+ Sbjct: 591 LASAGRYYENCPVQKKACEFLLSKQRPDGGW 621 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 30.3 bits (65), Expect = 0.29 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 486 FGSKPGSESHAGLIYCCVGTLSICKRMDALH 394 F S+P S SHA YC S+C + LH Sbjct: 458 FRSRPDSTSHASCTYCVARLRSVCNFVAFLH 488 >SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 27.5 bits (58), Expect = 2.1 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = -1 Query: 135 LSLLGNTSIKPVNPTYCMPQETIDRLKLKPQRLQ 34 +S LGN S+K + T+ + D+ KP+++Q Sbjct: 410 VSTLGNVSLKTPSDTFTLTNTDSDKKTSKPEKIQ 443 >SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mcl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 27.5 bits (58), Expect = 2.1 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = -1 Query: 522 EFVLSCMNFDGGFGSKPGSESHAGLIY 442 +F ++C++ +G + P +ES G+IY Sbjct: 471 KFEMACLDHEGALYASPATESSPGVIY 497 >SPCC320.12 ||SPCC330.17c|mitochondrial inner membrane peptidase Atp23|Schizosaccharomyces pombe|chr 3|||Manual Length = 185 Score = 26.6 bits (56), Expect = 3.6 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = +3 Query: 450 DQHGFPIQVWNRIHHQSSCN*AQTPQSC*CRW 545 D H F + WN + HQ+ + S CRW Sbjct: 93 DDHRFEVD-WNNLRHQACSEIRASSMSGECRW 123 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 25.4 bits (53), Expect = 8.4 Identities = 11/20 (55%), Positives = 17/20 (85%), Gaps = 1/20 (5%) Frame = -1 Query: 249 KKSLEEFILACQDYETG-GF 193 +K+++ +ILA QD+ETG GF Sbjct: 1909 RKAMDCYILASQDFETGEGF 1928 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,243,517 Number of Sequences: 5004 Number of extensions: 70882 Number of successful extensions: 201 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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