BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_pT_F06 (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom... 39 7e-04 SPBC428.17c |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.1 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 26 4.3 SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 25 9.9 >SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 38.7 bits (86), Expect = 7e-04 Identities = 26/67 (38%), Positives = 41/67 (61%), Gaps = 2/67 (2%) Frame = -2 Query: 447 ALKNM-LNDDRKTMELVLSVNGALRGLIRELTGNDIAKQCQAAGCICNLA-LGDSRAGVA 274 AL N+ +N + K LV+ +NG L LIR++ + QC A GCI NLA L ++++ +A Sbjct: 108 ALGNLAVNAENKA--LVVKLNG-LDLLIRQMMSPHVEVQCNAVGCITNLATLDENKSKIA 164 Query: 273 VTKSAGP 253 + + GP Sbjct: 165 HSGALGP 171 Score = 26.6 bits (56), Expect = 3.2 Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 2/90 (2%) Frame = -2 Query: 498 IVNILKTK-TSISVTELSALKNMLNDDRKTMELVLSVNGALRGLIRELTGNDIAKQCQAA 322 +V++L + T + +++ N+ D L S +R LI+ + + QCQAA Sbjct: 213 LVSLLPSSDTDVQYYCTTSISNIAVDAVHRKRLAQSEPKLVRSLIQLMDTSSPKVQCQAA 272 Query: 321 GCICNLALGDSRAGVAVTKS-AGPYLIAAL 235 + NLA D R + + +S A P L+ L Sbjct: 273 LALRNLA-SDERYQIEIVQSNALPSLLRLL 301 >SPBC428.17c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 602 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -2 Query: 453 LSALKNMLNDDRKTMELVLSVNGALRGLIRE 361 L N +DD T EL++ + G L GL+ E Sbjct: 458 LQKFSNFSSDDETTRELIILILGLLLGLVEE 488 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 26.2 bits (55), Expect = 4.3 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = -2 Query: 414 TMELVLSVNGALRGLIRELTGNDIAKQCQAAGCICNL 304 T+ LVL + G ++ ++ LTG I + +++ I N+ Sbjct: 66 TLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV 102 Score = 26.2 bits (55), Expect = 4.3 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = -2 Query: 414 TMELVLSVNGALRGLIRELTGNDIAKQCQAAGCICNL 304 T+ LVL + G ++ ++ LTG I + +++ I N+ Sbjct: 142 TLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV 178 Score = 26.2 bits (55), Expect = 4.3 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = -2 Query: 414 TMELVLSVNGALRGLIRELTGNDIAKQCQAAGCICNL 304 T+ LVL + G ++ ++ LTG I + +++ I N+ Sbjct: 218 TLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV 254 Score = 26.2 bits (55), Expect = 4.3 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = -2 Query: 414 TMELVLSVNGALRGLIRELTGNDIAKQCQAAGCICNL 304 T+ LVL + G ++ ++ LTG I + +++ I N+ Sbjct: 294 TLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV 330 >SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1210 Score = 25.0 bits (52), Expect = 9.9 Identities = 9/30 (30%), Positives = 17/30 (56%) Frame = -2 Query: 129 AVIIKLIFITAPFENQLFFNAPHFLYTPKT 40 A++ K I P + + FNAP+ + P++ Sbjct: 125 ALVAKCIVFNCPEDKENIFNAPNIIIGPRS 154 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,422,417 Number of Sequences: 5004 Number of extensions: 43492 Number of successful extensions: 79 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 70 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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