Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_E17
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 132 7e-32
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 28 1.3
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 27 2.3
SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces p... 27 2.3
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 27 3.1
SPCC794.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 4.1
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 26 5.4
SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta 5-->4-i... 26 7.2
SPCC4G3.07c |phf1|swp1, saf50|PHD finger containing protein Phf1... 26 7.2
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 132 bits (318), Expect = 7e-32
Identities = 65/156 (41%), Positives = 103/156 (66%), Gaps = 4/156 (2%)
Frame = -2
Query: 700 PKGTFNMDDFKRVYSNED-EAKSIPYFWEKFDPENYSIWYAEYKYPEELAK-VFMSCNLI 527
P G+F+++++KRVYSN+D + ++P+F+E FDPENYS+W +Y YPE+L + VFM+ NLI
Sbjct: 259 PNGSFDIEEYKRVYSNQDTRSGALPWFFEHFDPENYSVWKVDYSYPEDLKQPVFMTNNLI 318
Query: 526 TGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELV--FPLSSDWQVDYESYDWK 353
G FQRL+ RK F + GE+ ++TI+G +V +G + V F ++ DW SY +
Sbjct: 319 GGFFQRLEASRKYIFGCCVVIGENGDNTITGAFVIKGHDYVPAFDVAPDW----GSYTFT 374
Query: 352 KLDPSSEETKKLVQDYFSWNGTDKDGRKFNQGKIFK 245
KLD + E K ++D ++W+ +GR+ GK+ K
Sbjct: 375 KLDINKPEDKAFIEDAWAWD-KPIEGREVADGKVCK 409
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = -3
Query: 315 SRTTSRGTEPTKTVESSTRARYSSECRPTSS 223
S TTS T + T+ SSTR+ +SE TSS
Sbjct: 987 SSTTSGLTSSSSTIPSSTRSESNSESASTSS 1017
>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 873
Score = 27.5 bits (58), Expect = 2.3
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2
Query: 382 QVDYESYDWKKLD 344
QVDY SYDWK+ D
Sbjct: 298 QVDYLSYDWKETD 310
>SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 669
Score = 27.5 bits (58), Expect = 2.3
Identities = 17/52 (32%), Positives = 24/52 (46%)
Frame = -3
Query: 414 RSSCSRCRLIGRWTTSPTTGRNWILRARRPRNLSRTTSRGTEPTKTVESSTR 259
R S S R+I T P R++ ++ +N T EP +TVE TR
Sbjct: 531 RKSSSILRMISHSNTDPVELRSYNQSLQQNKNNEPTAVVPLEPEQTVELETR 582
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
Frame = +3
Query: 423 HTHTPEMVELLSS---PNRQTDAKACLRILSNLWNIPVIRL 536
+ H P V+ L+ PN + CL IL WN PV+ L
Sbjct: 80 YPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWN-PVLNL 119
>SPCC794.04c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 547
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = +3
Query: 156 IYI*MFLFCKVFYSYCMKCVYIGWTLVGIHLNILPWLNFLPSLSVPFHEK 305
+YI M + +FY Y +G ++GI + IL L P + V + +
Sbjct: 355 LYICMVGYPLIFYQYGFNAGEVGLAILGILVGILLGLALTPIIYVHYRRR 404
>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 476
Score = 26.2 bits (55), Expect = 5.4
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = -3
Query: 696 KVPSTWMISS--VSTPTKTKLNLFLTSGRSLTPRTIPFGMLNTNTPRNSLKCS*AVTLLR 523
K+PS++ I + + +P+ ++L + +L IP +LN+ PR +L TL+
Sbjct: 260 KLPSSFTIVNDPLKSPSSSRLRI---RNITLCADKIPRPLLNSKLPRKTLVLDLDETLIH 316
Query: 522 VCSRG 508
SRG
Sbjct: 317 SVSRG 321
>SPBC3F6.02c |||3 beta-hydroxysteroid dehydrogenase/delta
5-->4-isomerase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 340
Score = 25.8 bits (54), Expect = 7.2
Identities = 19/80 (23%), Positives = 33/80 (41%)
Frame = -1
Query: 347 GSFERGDQETCPGLLLVERNRQRR*KVQPGQDIQVNADQRPANVYTFHTITIKNFTKKKH 168
G F GD++ PG+L V +N Q K Q G ++ + N H + + N
Sbjct: 172 GLFGPGDRQLVPGMLSVLKNGQT--KFQLGDNLNLFDFTYIENAAYAHLLAMDNLLSSNP 229
Query: 167 SNVNRWMLAIFGTVLNLYNF 108
+ + G V+ ++F
Sbjct: 230 TANGQVFFITNGQVIYFWDF 249
>SPCC4G3.07c |phf1|swp1, saf50|PHD finger containing protein
Phf1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 461
Score = 25.8 bits (54), Expect = 7.2
Identities = 18/53 (33%), Positives = 24/53 (45%)
Frame = -3
Query: 624 SGRSLTPRTIPFGMLNTNTPRNSLKCS*AVTLLRVCSRG*TKCANRLSHPSAC 466
SGR + R + + T R S K VTL VC RG + +NR+ C
Sbjct: 163 SGRKIQ-RPVAYNPNATALKRKSRKVD-MVTLCSVCQRGHSPLSNRIVFCDGC 213
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,227,594
Number of Sequences: 5004
Number of extensions: 71556
Number of successful extensions: 251
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 248
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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