Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_E17
(800 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 103 2e-22
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 103 2e-22
At1g32190.1 68414.m03959 expressed protein 32 0.51
At3g06030.1 68416.m00688 NPK1-related protein kinase, putative (... 29 2.7
At5g08280.1 68418.m00975 hydroxymethylbilane synthase / porphobi... 28 6.3
At2g19660.1 68415.m02297 DC1 domain-containing protein contains ... 28 6.3
At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E... 28 6.3
At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.3
At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.3
At1g06210.2 68414.m00653 VHS domain-containing protein / GAT dom... 28 8.3
At1g06210.1 68414.m00654 VHS domain-containing protein / GAT dom... 28 8.3
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 103 bits (246), Expect = 2e-22
Identities = 52/158 (32%), Positives = 81/158 (51%), Gaps = 3/158 (1%)
Frame = -2
Query: 709 DXMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMS 539
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F++
Sbjct: 258 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVT 317
Query: 538 CNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYESYD 359
N + G QR+D RK +F + + G + + G+W++RG E+ D D E Y+
Sbjct: 318 LNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDMELYE 376
Query: 358 WKKLDPSSEETKKLVQDYFSWNGTDKDGRKFNQGKIFK 245
W K+D S E K+ V + +G K FK
Sbjct: 377 WTKVDISDEAQKERVSQMIE-DAEPFEGEALLDAKCFK 413
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 103 bits (246), Expect = 2e-22
Identities = 52/158 (32%), Positives = 81/158 (51%), Gaps = 3/158 (1%)
Frame = -2
Query: 709 DXMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEELAKVFMS 539
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E F++
Sbjct: 259 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDENMVSFVT 318
Query: 538 CNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYESYD 359
N + G QR+D RK +F + + G + + G+W++RG E+ D D E Y+
Sbjct: 319 LNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDMELYE 377
Query: 358 WKKLDPSSEETKKLVQDYFSWNGTDKDGRKFNQGKIFK 245
W K+D S E K+ V + +G K FK
Sbjct: 378 WTKVDISDEAQKERVSQMIE-DAEPFEGEALLDAKCFK 414
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 31.9 bits (69), Expect = 0.51
Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = -3
Query: 498 CANRLSHPSACLVKTTIP-PSLECGCGAERSSCSRC 394
C + L PS K P PS CGCG C +C
Sbjct: 296 CCSGLCRPSCSCPKPRCPKPSCSCGCGCGDCGCFKC 331
>At3g06030.1 68416.m00688 NPK1-related protein kinase, putative
(ANP3) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 3 GI:2342426
Length = 651
Score = 29.5 bits (63), Expect = 2.7
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -3
Query: 336 ARRPRNLSRTTSRGTEPTKTVESSTRARYSSEC 238
++R N SR GTEPT+ ES+ + +S C
Sbjct: 580 SKRTLNTSRVMQSGTEPTQVNESTKKGVNNSRC 612
>At5g08280.1 68418.m00975 hydroxymethylbilane synthase /
porphobilinogen deaminase, chloroplast /
pre-uroporphyrinogen synthase identical to SP|Q43316
Length = 382
Score = 28.3 bits (60), Expect = 6.3
Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = +2
Query: 224 LDVGRHSLEYL-ALVELSTVFVGSVPREVVLDKFLGLLARRIQFLPVVGLVVHLPIRRQR 400
+D+ HS++ + + T+ ++PRE V D F+ L A + LP +V +RR+
Sbjct: 148 IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLRRKS 207
Query: 401 E 403
+
Sbjct: 208 Q 208
>At2g19660.1 68415.m02297 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 662
Score = 28.3 bits (60), Expect = 6.3
Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = +3
Query: 135 ENCQHPPIYI*MFL-FCKVFYSYCMKCVYIGWTLVGIHLNIL 257
E + PPI+ L F + Y C C Y+G+ G H N L
Sbjct: 2 EKVEVPPIHNHPLLPFTRFSYKACKGCGYVGYIYGGYHCNEL 43
>At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E2;
identical to gi:2689242, SP:P42745
Length = 152
Score = 28.3 bits (60), Expect = 6.3
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = +3
Query: 405 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 518
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 6.3
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = +3
Query: 405 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 518
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 6.3
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = +3
Query: 405 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 518
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g06210.2 68414.m00653 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 279
Score = 27.9 bits (59), Expect = 8.3
Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 5/83 (6%)
Frame = -1
Query: 353 ETGSFERGDQETCPGLLLVERNRQRR*KVQPGQDIQV-NADQRPAN----VYTFHTITIK 189
E G RG++ + PG +E QR V P V N +Q + Y F ++IK
Sbjct: 167 ENGLHARGEENSMPGQSSLESLMQRPVPVPPPGSYPVPNQEQALGDDDGLDYNFGNLSIK 226
Query: 188 NFTKKKHSNVNRWMLAIFGTVLN 120
+ KK+ + R L + ++LN
Sbjct: 227 D--KKEQIEITRNSLELLSSMLN 247
>At1g06210.1 68414.m00654 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 383
Score = 27.9 bits (59), Expect = 8.3
Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 5/83 (6%)
Frame = -1
Query: 353 ETGSFERGDQETCPGLLLVERNRQRR*KVQPGQDIQV-NADQRPAN----VYTFHTITIK 189
E G RG++ + PG +E QR V P V N +Q + Y F ++IK
Sbjct: 167 ENGLHARGEENSMPGQSSLESLMQRPVPVPPPGSYPVPNQEQALGDDDGLDYNFGNLSIK 226
Query: 188 NFTKKKHSNVNRWMLAIFGTVLN 120
+ KK+ + R L + ++LN
Sbjct: 227 D--KKEQIEITRNSLELLSSMLN 247
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,900,159
Number of Sequences: 28952
Number of extensions: 377959
Number of successful extensions: 1111
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1068
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1107
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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