BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_pT_E11 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 276 2e-75 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 136 4e-33 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 125 5e-30 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 120 2e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 94 2e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 78 1e-15 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 73 4e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 2e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 4e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.6 SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|... 25 7.9 SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 7.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 276 bits (677), Expect = 2e-75 Identities = 127/145 (87%), Positives = 136/145 (93%) Frame = -2 Query: 699 AASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIR 520 A S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIR Sbjct: 231 AQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIR 290 Query: 519 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLS 340 KDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLS Sbjct: 291 KDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLS 350 Query: 339 TFQQMWISKEEYDESGPGIVHRKCF 265 TFQQMWISK+EYDESGPGIV+RKCF Sbjct: 351 TFQQMWISKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 136 bits (328), Expect = 4e-33 Identities = 60/137 (43%), Positives = 93/137 (67%) Frame = -2 Query: 675 SYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTV 496 +Y+LPD Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N V Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303 Query: 495 MSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS 316 +SGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ Sbjct: 304 LSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLIT 363 Query: 315 KEEYDESGPGIVHRKCF 265 EEY ++ ++ R+ F Sbjct: 364 SEEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 125 bits (302), Expect = 5e-30 Identities = 63/146 (43%), Positives = 96/146 (65%), Gaps = 12/146 (8%) Frame = -2 Query: 693 STSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKD 514 +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK Sbjct: 233 TTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKY 292 Query: 513 LYANTVMSGGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWI 367 LY V+SGG++MY G+ R++KEI L P+ + K+KI P R+++V+I Sbjct: 293 LYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFI 352 Query: 366 GGSILAS-LSTFQQMWISKEEYDESG 292 GG++LA ++ MW+SK E++E G Sbjct: 353 GGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 120 bits (289), Expect = 2e-28 Identities = 64/146 (43%), Positives = 84/146 (57%), Gaps = 11/146 (7%) Frame = -2 Query: 696 ASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESC---------GIHETVYNSI 544 AS+ K +E PDG G ERFR E LF PSF S G+HE VY SI Sbjct: 273 ASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSI 332 Query: 543 MKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKIKII-APPERKYSVW 370 + CD ++R L N V++GGT++ PG+++R+Q E+ LA S I + +VW Sbjct: 333 LACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVW 392 Query: 369 IGGSILASLSTFQQMWISKEEYDESG 292 GGSILASL FQ +W+SK+EYDE G Sbjct: 393 FGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 93.9 bits (223), Expect = 2e-20 Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%) Frame = -2 Query: 573 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAP 394 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 393 P---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGP---GIVHRKC 268 ER Y+ W+GGSIL+SL TF Q+WIS++EY+E G ++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 77.8 bits (183), Expect = 1e-15 Identities = 43/128 (33%), Positives = 64/128 (50%) Frame = -2 Query: 654 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTM 475 QV+ + NERF PE LF PS + + GI E V S+ +I+ L N V GG Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331 Query: 474 YPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDES 295 +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390 Query: 294 GPGIVHRK 271 G I+ RK Sbjct: 391 GANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 72.9 bits (171), Expect = 4e-14 Identities = 41/140 (29%), Positives = 67/140 (47%), Gaps = 15/140 (10%) Frame = -2 Query: 648 ITIGNERFRCPEALFQPSFLGMESCG-IHETVYNSIMKCDVDIRKDLYANTVMSGGTTMY 472 I +G ERF PE F P + + E V N + +D+RK LY N V+SGG+T++ Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339 Query: 471 PGIADRMQKEITALAPSTI--------------KIKIIAPPERKYSVWIGGSILASLSTF 334 +R+Q+++ + I + +I+ ++ +VW GGS+LA F Sbjct: 340 KNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399 Query: 333 QQMWISKEEYDESGPGIVHR 274 +K +Y+E G I R Sbjct: 400 GSYCHTKADYEEYGASIARR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.9 bits (166), Expect = 2e-13 Identities = 32/115 (27%), Positives = 62/115 (53%) Frame = -2 Query: 633 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 454 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 453 MQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGP 289 +++E+T++ P I + W G S + F+ +++EEY E GP Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = -2 Query: 519 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILA 349 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 348 SLSTFQQMWISKEEYDESGPGIVHRK 271 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.5 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = -2 Query: 372 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 271 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -2 Query: 447 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 337 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC2D10.03c |||DUF866 domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +2 Query: 209 ELGRAQHHTAPGSRGRAA*KHLRWT 283 E+ R++ H+ PGS+G A +L WT Sbjct: 46 EISRSETHSIPGSKGEA---NLIWT 67 >SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/43 (34%), Positives = 20/43 (46%) Frame = -1 Query: 568 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 440 PR+ V R+R P P + R V PR RRQ +G+ Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,600,980 Number of Sequences: 5004 Number of extensions: 49914 Number of successful extensions: 150 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 135 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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