Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_E10
(783 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 171 6e-43
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 165 2e-41
At1g53510.1 68414.m06068 mitogen-activated protein kinase, putat... 35 0.053
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 31 0.86
At3g10180.1 68416.m01219 kinesin motor protein-related similar t... 28 6.1
At3g58160.1 68416.m06485 myosin heavy chain, putative similar to... 28 8.0
At2g46980.2 68415.m05869 expressed protein 28 8.0
At2g46980.1 68415.m05868 expressed protein 28 8.0
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 171 bits (415), Expect = 6e-43
Identities = 90/194 (46%), Positives = 119/194 (61%), Gaps = 1/194 (0%)
Frame = -1
Query: 780 DILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQG-LTRAFRNIPGVEXXXXXXXXX 604
D K S +R GKGKMRNRR I RKGPL+++ + + +AFRN+PGVE
Sbjct: 184 DAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVYGTEGSKIVKAFRNLPGVELCHVERLNL 243
Query: 603 XXLAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDE 424
LAPGGHLGRFVIWT+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ SDE
Sbjct: 244 LKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDE 303
Query: 423 IRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALADAEK 244
I+ V+ K RA K NPL N MLKLNPYA KR ++L +R K A+K
Sbjct: 304 IQSVVNPIKKDAKRAVLKKNPLKNLNVMLKLNPYAKTAKRMSLLAEAQRVKAKKEKLAKK 363
Query: 243 SGLKLSKRNPAMKA 202
+ A+KA
Sbjct: 364 RKTVTKEEALAIKA 377
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 165 bits (402), Expect = 2e-41
Identities = 87/194 (44%), Positives = 116/194 (59%), Gaps = 1/194 (0%)
Frame = -1
Query: 780 DILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQG-LTRAFRNIPGVEXXXXXXXXX 604
D K S +R GKGKMRNRR I RKGPL++F + + +AFRN+PGVE
Sbjct: 185 DAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVFGTEGAKIVKAFRNLPGVELCHVERLNL 244
Query: 603 XXLAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDE 424
LAPGGHLGRFVIWT+SAF +L+ ++GS++ PS++KK + LP+ KM N DL R++ SDE
Sbjct: 245 LKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDE 304
Query: 423 IRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALADAEK 244
++ V+ RA K NPL N M KLNPYA KR ++L R K +K
Sbjct: 305 VQSVVNPIKDGSKRAVLKKNPLKNLNVMFKLNPYAKTAKRMSLLAEASRVKAKKEKLEKK 364
Query: 243 SGLKLSKRNPAMKA 202
+ + A+KA
Sbjct: 365 RKVVTKEEAQAIKA 378
>At1g53510.1 68414.m06068 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK18) mitogen-activated protein
kinase (MAPK)(AtMPK18), PMID:12119167
Length = 603
Score = 35.1 bits (77), Expect = 0.053
Identities = 32/99 (32%), Positives = 47/99 (47%), Gaps = 6/99 (6%)
Frame = -1
Query: 456 TDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYA-AVLKRKAILELRR 280
TDL KS+ I V ++ + RK NP+T ++ K +P A +L+R + +
Sbjct: 232 TDLLGTPKSETISGVRNDKARKYLTEMRKKNPVTFSQKFSKADPLALRLLQRLLAFDPKD 291
Query: 279 RKN-LKALADAEKSGLKLSKRNPA----MKAEKLRERRR 178
R +ALAD GL +R P+ K E ERRR
Sbjct: 292 RPTPAEALADPYFKGLSKIEREPSSQQISKMEFEFERRR 330
>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
domain-containing protein weak similarity to SP|P45974
Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
{Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
domain
Length = 413
Score = 31.1 bits (67), Expect = 0.86
Identities = 33/110 (30%), Positives = 49/110 (44%), Gaps = 1/110 (0%)
Frame = -1
Query: 501 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 322
K N N+ K A T LK+ E+R+ RA K+ K K +++
Sbjct: 162 KVPSNSNVGPAKPALTPEEVKLKAQELRE--RARKKK--EEEEKRMEREREKERIRIGK- 216
Query: 321 AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLR-ERRRK 175
+L+ K + E+ RK L L AEK K ++ K E+ + ERRRK
Sbjct: 217 -ELLEAKRMEEVNERKRLMFLRKAEKEEEKRAREKIRQKLEEDKAERRRK 265
>At3g10180.1 68416.m01219 kinesin motor protein-related similar to
centromere protein E GB:4502781 [Homo sapiens]
Length = 1348
Score = 28.3 bits (60), Expect = 6.1
Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 2/110 (1%)
Frame = -1
Query: 507 PSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLN 328
P + K + QP + T ++ + + + K ++ + +TN + ++
Sbjct: 278 PYRDSKLTRILQPALGGNANTAIICNITLAPIHADETKSSLQFASRALRVTNCAHVNEIL 337
Query: 327 PYAAVLKR--KAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRER 184
AA+LKR K I ELR + +E+ L L RN +K+E RER
Sbjct: 338 TDAALLKRQKKEIEELRSKLKTSHSDHSEEEILNL--RNTLLKSELERER 385
>At3g58160.1 68416.m06485 myosin heavy chain, putative similar to
myosin heavy chain [Arabidopsis thaliana]
gi|602328|emb|CAA84067.
Length = 1242
Score = 27.9 bits (59), Expect = 8.0
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = -1
Query: 270 LKALADAEKSGLKLSKRNPAMKAEKLRERRRKNI 169
LKAL EK +S+R A +A +L ERRRK +
Sbjct: 1092 LKALLQVEKQRADISERKCA-EARELGERRRKRL 1124
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 27.9 bits (59), Expect = 8.0
Identities = 29/119 (24%), Positives = 53/119 (44%), Gaps = 4/119 (3%)
Frame = -1
Query: 513 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLK 334
+TP +K NF L Q K +N D L+K + ++ ATR+ P+T + +
Sbjct: 218 ETPEVEKTNFKLSQDKGSNDD--PLIKPRHNSDSIETDSESPENATRR--PVTRSLLQRR 273
Query: 333 LNPYAAVLKRKAILELRRR--KNLKALADAEKS--GLKLSKRNPAMKAEKLRERRRKNI 169
+ K KA L R+ + + ++ E+ G + N ++ +K R RR+ +
Sbjct: 274 VGAKGVQKKTKAGANLGRKCTEQVNSVFSFEEGLRGKIGTAVNSSVMPKKQRGRRKNTV 332
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 27.9 bits (59), Expect = 8.0
Identities = 29/119 (24%), Positives = 53/119 (44%), Gaps = 4/119 (3%)
Frame = -1
Query: 513 KTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLK 334
+TP +K NF L Q K +N D L+K + ++ ATR+ P+T + +
Sbjct: 218 ETPEVEKTNFKLSQDKGSNDD--PLIKPRHNSDSIETDSESPENATRR--PVTRSLLQRR 273
Query: 333 LNPYAAVLKRKAILELRRR--KNLKALADAEKS--GLKLSKRNPAMKAEKLRERRRKNI 169
+ K KA L R+ + + ++ E+ G + N ++ +K R RR+ +
Sbjct: 274 VGAKGVQKKTKAGANLGRKCTEQVNSVFSFEEGLRGKIGTAVNSSVMPKKQRGRRKNTV 332
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,723,678
Number of Sequences: 28952
Number of extensions: 275300
Number of successful extensions: 786
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 770
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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