Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_D12
(579 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 91 8e-20
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 82 5e-17
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 64 1e-11
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 52 5e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 46 4e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.86
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 26 4.6
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 25 6.1
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 6.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 91.5 bits (217), Expect = 8e-20
Identities = 50/134 (37%), Positives = 77/134 (57%), Gaps = 6/134 (4%)
Frame = -2
Query: 545 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 378
+ +Q+AEF+EAF D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 377 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 204
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV +
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125
Query: 203 AYDQMDIDDKGYID 162
+ D D G I+
Sbjct: 126 MIREADTDGDGVIN 139
Score = 28.7 bits (61), Expect = 0.65
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = -2
Query: 290 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLTA 132
D G ID +E L D + +EV EA+ D D GYI +L +LT+
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 82.2 bits (194), Expect = 5e-17
Identities = 45/126 (35%), Positives = 65/126 (51%), Gaps = 2/126 (1%)
Frame = -2
Query: 578 KRTGSNVFXMFSQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVG 399
KR S F + Q+ E KEAF +D D DG IG+ D++ SL + ASE ++ M
Sbjct: 32 KRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Query: 398 EASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTWGDKF 225
+ PIN LT + + S +D ++ AF TFD + GKI +R AL + GD+
Sbjct: 92 SINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSMGDRM 150
Query: 224 SADEVD 207
EV+
Sbjct: 151 DPQEVE 156
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 64.1 bits (149), Expect = 1e-11
Identities = 39/140 (27%), Positives = 71/140 (50%), Gaps = 2/140 (1%)
Frame = -2
Query: 545 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 366
S++Q E KEAF D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 365 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 192
++ +N++ E++ I AF+ FD++ G I++ + + T G+K S +EV +
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122
Query: 191 MDIDDKGYIDTTKLIAMLTA 132
D + G D + + A
Sbjct: 123 ADPTNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 52.4 bits (120), Expect = 5e-08
Identities = 35/142 (24%), Positives = 67/142 (47%), Gaps = 6/142 (4%)
Frame = -2
Query: 545 SQKQVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 378
+++Q + EAF+ D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 377 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 204
+ + ++ D + + AF+ F DE GKI LR + E++
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150
Query: 203 AYDQMDIDDKGYIDTTKLIAML 138
++ D+D G I+ + IA++
Sbjct: 151 MIEEFDLDQDGEINEQEFIAIM 172
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 46.0 bits (104), Expect = 4e-06
Identities = 33/136 (24%), Positives = 63/136 (46%), Gaps = 4/136 (2%)
Frame = -2
Query: 524 FKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 345
+K+AF D G I K + + G+ + E+ E+ ++ Q L + NR
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66
Query: 344 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 177
+G + + + F+ FD++ G I LR+ L + G+K S +E+DE + + D
Sbjct: 67 PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126
Query: 176 KGYIDTTKLIAMLTAS 129
G ++ + M+ A+
Sbjct: 127 -GMVNYHDFVQMILAN 141
Score = 39.9 bits (89), Expect = 3e-04
Identities = 18/42 (42%), Positives = 25/42 (59%)
Frame = -2
Query: 527 EFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 402
EF + FQ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 0.86
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -2
Query: 218 DEVDEAYDQMDIDDKGYID 162
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
Score = 25.0 bits (52), Expect = 8.0
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -2
Query: 527 EFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 402
E +EAF D G I DLR + LG ++++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.8 bits (54), Expect = 4.6
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -2
Query: 536 QVAEFKEAFQXMDHDKDGIIGKNDLRATFDSLGRLASE 423
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.4 bits (53), Expect = 6.1
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = +1
Query: 394 ASPTISSSSFSDASLPRESKVARKSF 471
+SP++ +++ + P++S V+RKSF
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSF 60
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 6.1
Identities = 21/49 (42%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +1
Query: 328 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKS 468
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS 590
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,793,170
Number of Sequences: 5004
Number of extensions: 28357
Number of successful extensions: 147
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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