BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_pT_D04 (606 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 349 2e-97 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 347 1e-96 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 2e-36 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 79 5e-16 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.13 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.13 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.40 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 29 0.70 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 0.92 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.2 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 3.7 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.7 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 4.9 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 4.9 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 6.5 SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schiz... 25 6.5 SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 8.6 SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 8.6 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 349 bits (857), Expect = 2e-97 Identities = 153/193 (79%), Positives = 173/193 (89%) Frame = -3 Query: 604 NVDLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 425 NVDL EFQ NLVPYPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR Sbjct: 253 NVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 312 Query: 424 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 245 G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG + Sbjct: 313 TGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGI 372 Query: 244 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 65 AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL Sbjct: 373 AKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 432 Query: 64 EKDYEEVGMDSAE 26 E+DYEEVG DS + Sbjct: 433 ERDYEEVGQDSMD 445 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 347 bits (852), Expect = 1e-96 Identities = 154/193 (79%), Positives = 171/193 (88%) Frame = -3 Query: 604 NVDLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 425 NVDL EFQ NLVPYPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR Sbjct: 249 NVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPR 308 Query: 424 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 245 G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++ Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368 Query: 244 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 65 AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428 Query: 64 EKDYEEVGMDSAE 26 E+DYEEVG DS E Sbjct: 429 ERDYEEVGQDSME 441 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 146 bits (354), Expect = 2e-36 Identities = 72/196 (36%), Positives = 114/196 (58%), Gaps = 3/196 (1%) Frame = -3 Query: 604 NVDLTEFQXNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPR 425 N DL + N+VP+PR+HF +V +AP+ + + + +SV E+T F+ N MV DPR Sbjct: 247 NSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPR 306 Query: 424 HGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 245 HG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP DL Sbjct: 307 HGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL 361 Query: 244 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDL 74 + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL Sbjct: 362 ---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL 418 Query: 73 AALEKDYEEVGMDSAE 26 + + Y+E G+D + Sbjct: 419 VSEYQQYQEAGIDEGD 434 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 79.0 bits (186), Expect = 5e-16 Identities = 51/192 (26%), Positives = 94/192 (48%), Gaps = 7/192 (3%) Frame = -3 Query: 604 NVDLTEFQXNLVPYPRIHFPLVTYAPVISA---EKAYHEQLSVAEITNACFEPANQMVKC 434 N DL +L+P PR HF L +Y P + E + +V ++ P NQMV Sbjct: 250 NNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSV 309 Query: 433 DP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVP 257 +P + +++ + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + Sbjct: 310 NPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKT 369 Query: 256 GGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEA 86 ++ + ML+N T+IA + R ++D + + AF+ Y E + E EF + Sbjct: 370 NHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSS 424 Query: 85 REDLAALEKDYE 50 R+ +A L +YE Sbjct: 425 RDVVADLINEYE 436 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 31.1 bits (67), Expect = 0.13 Identities = 24/89 (26%), Positives = 33/89 (37%) Frame = -1 Query: 522 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 343 S+P+ P P S + P P + S VV P + RP++P Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPV-----APEVPSAPQRPAAPVVPEAPSVPQRPAVPV 567 Query: 342 KPSVLSNSSTGVQPVSRSVSTTSHPPWCP 256 P LS V PV+ V + PP P Sbjct: 568 VPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 Score = 25.4 bits (53), Expect = 6.5 Identities = 23/90 (25%), Positives = 31/90 (34%) Frame = -1 Query: 522 SLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPS 343 S+P+RP + P S P P + +A S P+ P P Sbjct: 558 SVPQRPAVPVVPEALSVPQPPVAPVA--PEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPE 615 Query: 342 KPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 253 PSV + V P + SV P PE Sbjct: 616 VPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 645 Score = 25.0 bits (52), Expect = 8.6 Identities = 14/45 (31%), Positives = 18/45 (40%) Frame = -1 Query: 390 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 256 VV P + RP++P P S PV V + PP P Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 31.1 bits (67), Expect = 0.13 Identities = 30/108 (27%), Positives = 44/108 (40%), Gaps = 2/108 (1%) Frame = -1 Query: 585 SRXTWCLTPVSTSHWSRTRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPVMAST 412 S T C T S T S+ P PT+ S +P + T+ ++ + +TPV ++ Sbjct: 388 STSTSCTTSTSIPP---TGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTP 444 Query: 411 WLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHP 268 CT TS P T + S P +N +T S TS P Sbjct: 445 LATTNCTTSTSVPY--TSTPVTSTPLTTTNCTTSTSIPYTSTPVTSTP 490 Score = 29.5 bits (63), Expect = 0.40 Identities = 29/105 (27%), Positives = 49/105 (46%) Frame = -1 Query: 588 SSRXTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTW 409 S+ + TPV++S++ T SS P T P + T+ ++ + +TPV ++ Sbjct: 499 STSVPYTSTPVTSSNY--TISSSTPVTST------PVTTTNCTTSTSVLYTSTPVTSTPL 550 Query: 408 LAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 274 CT TS P T P S ++ S++ PV+ + TTS Sbjct: 551 ATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTSTPVTTTNCTTS 594 Score = 29.1 bits (62), Expect = 0.53 Identities = 27/107 (25%), Positives = 48/107 (44%) Frame = -1 Query: 594 SPSSRXTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 415 +P+ T TP++T++ T +S+P T + +P + T+ ++ + +TPV ++ Sbjct: 410 TPTVPPTSSSTPLTTTNC--TTSTSVPYTSTPVT-STPLATTNCTTSTSVPYTSTPVTST 466 Query: 414 TWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 274 CT TS P T + S P +N +T S TS Sbjct: 467 PLTTTNCTTSTSIPY--TSTPVTSTPLTTTNCTTSTSVPYTSTPVTS 511 Score = 25.8 bits (54), Expect = 4.9 Identities = 27/98 (27%), Positives = 49/98 (50%), Gaps = 1/98 (1%) Frame = -1 Query: 564 TPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVV 385 TP++T++ T +S+P T + +P + T+ ++ + +TPV +S + T V Sbjct: 466 TPLTTTNC--TTSTSIPYTSTPVT-STPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPV 522 Query: 384 TSYPRM*TRPSLPSKPSVLSNSSTGVQ-PVSRSVSTTS 274 TS P T + + SVL S+ P++ + TTS Sbjct: 523 TSTPV--TTTNCTTSTSVLYTSTPVTSTPLATTNCTTS 558 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 29.5 bits (63), Expect = 0.40 Identities = 26/94 (27%), Positives = 46/94 (48%) Frame = -1 Query: 594 SPSSRXTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 415 S SS + + STS + T SS RP +S S +H SS ++ ++ PV ++ Sbjct: 364 SSSSFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKS-SSAPVSSA 422 Query: 414 TWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSST 313 + + +S+ + SL SKP + ++SS+ Sbjct: 423 FYHNSTSSRSSSHSSSHSLSSLSSKPILTASSSS 456 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.7 bits (61), Expect = 0.70 Identities = 20/73 (27%), Positives = 34/73 (46%) Frame = +2 Query: 350 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGKLFMVGLLGRDDW 529 S G +LGY ++ A++V+A + V HL+ G D + K G D Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK-------GLDML 459 Query: 530 RVRDQWEVDTGVR 568 R Q+E++ G++ Sbjct: 460 VDRVQYELEAGLQ 472 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 0.92 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = -3 Query: 478 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 353 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = -1 Query: 591 PSSRXTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 445 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 3.7 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -1 Query: 528 QSSLPRRPTMNSFPSPRSQTHASSPPTR 445 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 3.7 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = -1 Query: 354 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 268 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 4.9 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = +1 Query: 373 WVRRHHGTAYSKPCTCHDGG 432 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 4.9 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = -1 Query: 567 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 415 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 6.5 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = -1 Query: 357 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 262 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPAC23E2.02 |lsd2|swm2, saf140|histone demethylase SWIRM2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1273 Score = 25.4 bits (53), Expect = 6.5 Identities = 22/84 (26%), Positives = 36/84 (42%) Frame = -1 Query: 561 PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVT 382 P+ SH S + S+ R + + H S PT+ +AT + S AV T Sbjct: 253 PLDASHLSSQQLLSMYRDQVSHGVTPSTFRNHESFMPTQLVSATELSKSVDNAVLPIPPT 312 Query: 381 SYPRM*TRPSLPSKPSVLSNSSTG 310 + P + P S P + S +++G Sbjct: 313 TAPAV-VSPPASSFPLMSSAATSG 335 >SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 25.0 bits (52), Expect = 8.6 Identities = 15/37 (40%), Positives = 16/37 (43%) Frame = -1 Query: 375 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 265 PR LP K VL T + VSR TTS P Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329 >SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 530 Score = 25.0 bits (52), Expect = 8.6 Identities = 19/60 (31%), Positives = 26/60 (43%) Frame = -1 Query: 435 ATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 256 +TP ++S A VV+S T + S S ++SS P S SVS W P Sbjct: 245 STPAVSSA-AASSSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSVSGKRGLAWIP 303 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,625,664 Number of Sequences: 5004 Number of extensions: 56082 Number of successful extensions: 228 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 205 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 225 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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