Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_pT_B24
(817 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 120 9e-28
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 120 1e-27
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 116 2e-26
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 114 6e-26
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 36 0.032
At1g27000.1 68414.m03292 bZIP family transcription factor 36 0.032
At2g22560.1 68415.m02674 kinase interacting protein-related simi... 34 0.13
At4g15545.1 68417.m02375 expressed protein 33 0.17
At1g50200.1 68414.m05629 aminoacyl-tRNA synthetase family protei... 31 0.91
At3g44400.1 68416.m04770 disease resistance protein (TIR-NBS-LRR... 31 1.2
At4g31170.2 68417.m04425 protein kinase family protein contains ... 30 1.6
At4g31170.1 68417.m04424 protein kinase family protein contains ... 30 1.6
At2g24360.1 68415.m02911 serine/threonine/tyrosine kinase, putat... 30 2.1
At3g19370.1 68416.m02457 expressed protein 29 2.8
At5g53570.1 68418.m06656 RabGAP/TBC domain-containing protein si... 29 3.7
At3g58840.1 68416.m06558 expressed protein 29 3.7
At3g57990.1 68416.m06463 expressed protein 29 4.9
At2g18260.1 68415.m02129 syntaxin-related protein, putative (SYP... 29 4.9
At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family ... 28 6.4
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 120 bits (290), Expect = 9e-28
Identities = 57/104 (54%), Positives = 74/104 (71%), Gaps = 3/104 (2%)
Frame = -2
Query: 315 AYNAKKAKKPVLIAKSNIILDVKPWDDETDMAALEQAVRSISTDGLLWGAAKLVPLAYGI 136
A + K + K KS++++D+KPWDDETDM LE+AV+SI +GL WGA+KLVP+ YGI
Sbjct: 128 AASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGASKLVPVGYGI 187
Query: 135 HKLQISCVVEDDKVSIDWLTEE---IEKNEDYVQSVDIAAFNKV 13
KLQI C + DD VSID + EE +E +YVQS DI AFNK+
Sbjct: 188 KKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 120 bits (289), Expect = 1e-27
Identities = 57/104 (54%), Positives = 73/104 (70%), Gaps = 3/104 (2%)
Frame = -2
Query: 315 AYNAKKAKKPVLIAKSNIILDVKPWDDETDMAALEQAVRSISTDGLLWGAAKLVPLAYGI 136
A + K + K KS++++D+KPWDDETDM LE+AVRSI +GL WGA+KLVP+ YGI
Sbjct: 128 AASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYGI 187
Query: 135 HKLQISCVVEDDKVSIDWLTEE---IEKNEDYVQSVDIAAFNKV 13
KL I C + DD VSID + EE +E +YVQS DI AFNK+
Sbjct: 188 KKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 116 bits (278), Expect = 2e-26
Identities = 56/100 (56%), Positives = 70/100 (70%), Gaps = 3/100 (3%)
Frame = -2
Query: 303 KKAKKPVLIAKSNIILDVKPWDDETDMAALEQAVRSISTDGLLWGAAKLVPLAYGIHKLQ 124
K KK KS+++L+VKPWDDETDM LE+AVRS+ GL WGA+KLVP+ YGI KL
Sbjct: 129 KDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYGIKKLT 188
Query: 123 ISCVVEDDKVSIDWLTEE---IEKNEDYVQSVDIAAFNKV 13
I + DD VS+D L E+ E N +Y+QSVDI AFNK+
Sbjct: 189 IMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIVAFNKI 228
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 114 bits (275), Expect = 6e-26
Identities = 55/100 (55%), Positives = 68/100 (68%), Gaps = 3/100 (3%)
Frame = -2
Query: 303 KKAKKPVLIAKSNIILDVKPWDDETDMAALEQAVRSISTDGLLWGAAKLVPLAYGIHKLQ 124
K KKP KS++++DVKPWDDETDM LE+AVR + GL WGA+KLVP+ YGI KL
Sbjct: 125 KDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGIKKLT 184
Query: 123 ISCVVEDDKVSIDWLTEEI---EKNEDYVQSVDIAAFNKV 13
I + DD VS D L E+ E N +Y+QS DI AFNK+
Sbjct: 185 IMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFNKI 224
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 35.9 bits (79), Expect = 0.032
Identities = 26/79 (32%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = -2
Query: 279 IAKSNIILDVKPWDDETDMAALEQAVRSISTDGLLW--GAAKLVPLAYGIHKLQISCVVE 106
+ SN++ D+K WD+ M AL + + T G W +L+ G LQ C E
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQ--CGRE 592
Query: 105 DDKVSIDWLTEEIEKNEDY 49
D D L EE+ K E Y
Sbjct: 593 DSGSLFDLLVEEM-KRERY 610
>At1g27000.1 68414.m03292 bZIP family transcription factor
Length = 304
Score = 35.9 bits (79), Expect = 0.032
Identities = 17/72 (23%), Positives = 38/72 (52%)
Frame = -2
Query: 672 VAKARQHIKNSLECMDSVATLAGVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVR 493
+A A++H+ ++ +D + E++++VIS L+ ++ L NL+ L +
Sbjct: 147 LAAAKRHLTQRIQNLDDKVEKQIDLSKEINSQVISARENISSLEMDLESLHNLITGLDGK 206
Query: 492 VETLESSSSITS 457
++TLE +T+
Sbjct: 207 LDTLEYKQDVTN 218
>At2g22560.1 68415.m02674 kinase interacting protein-related similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]; weak similarity to Myosin II heavy chain,
non muscle (Swiss-Prot:P08799) [Dictyostelium discoideum
Length = 891
Score = 33.9 bits (74), Expect = 0.13
Identities = 22/71 (30%), Positives = 35/71 (49%)
Frame = -2
Query: 666 KARQHIKNSLECMDSVATLAGVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVE 487
K R+H ++ + +A + EL KVISLE+ I LRN LQ ++
Sbjct: 279 KIREHFESGANSSLNGTDMAEKVD-ELVNKVISLESAVSSQTALIQRLRNETNGLQTQIS 337
Query: 486 TLESSSSITSD 454
TLE+ ++ +D
Sbjct: 338 TLETDKALLAD 348
>At4g15545.1 68417.m02375 expressed protein
Length = 337
Score = 33.5 bits (73), Expect = 0.17
Identities = 17/46 (36%), Positives = 29/46 (63%)
Frame = -2
Query: 603 VPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVETLESSSS 466
+ + LST+V +LE+E+ DL++ + + LQ VE+LE+S S
Sbjct: 46 ITSIALSTRVSALESESSDLRELLAEKEKEFEELQSHVESLEASLS 91
>At1g50200.1 68414.m05629 aminoacyl-tRNA synthetase family protein
contains Pfam profiles: PF01411 tRNA synthetases class II
(A), PF02272 DHHA1 domain
Length = 1003
Score = 31.1 bits (67), Expect = 0.91
Identities = 17/60 (28%), Positives = 34/60 (56%)
Frame = -2
Query: 645 NSLECMDSVATLAGVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVETLESSSS 466
++L+ A + ++ TK+ SL+NE + +K I + +NL S+++ E ES++S
Sbjct: 832 SALKSRVDAAIIPAAKKADIRTKIASLQNEVRKAQKKIAE-QNLKKSVKLATEAAESAAS 890
>At3g44400.1 68416.m04770 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1007
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = -2
Query: 666 KARQHIKNSLECMDSVATLAGVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVE 487
K ++HI+ + ++ VAT+AG + + + +E + D+ K DD + ++ R E
Sbjct: 166 KTKEHIERWRKALEDVATIAGYHSHKWCDEAEMIEKISTDVSKDFDDFVGMAAHME-RTE 224
Query: 486 TL 481
L
Sbjct: 225 QL 226
>At4g31170.2 68417.m04425 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 412
Score = 30.3 bits (65), Expect = 1.6
Identities = 15/65 (23%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -2
Query: 201 RSISTDGLLWGAAKLVPLA-YGIHKLQISCV-VEDDKVSIDWLTEEIEKNEDYVQSVDIA 28
R + +D LL A + + +A +G+ ++++ + + + W+ E+ ++ Y Q VD+
Sbjct: 256 RDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMIQHRPYTQKVDVY 315
Query: 27 AFNKV 13
+F V
Sbjct: 316 SFGIV 320
>At4g31170.1 68417.m04424 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 412
Score = 30.3 bits (65), Expect = 1.6
Identities = 15/65 (23%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -2
Query: 201 RSISTDGLLWGAAKLVPLA-YGIHKLQISCV-VEDDKVSIDWLTEEIEKNEDYVQSVDIA 28
R + +D LL A + + +A +G+ ++++ + + + W+ E+ ++ Y Q VD+
Sbjct: 256 RDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMIQHRPYTQKVDVY 315
Query: 27 AFNKV 13
+F V
Sbjct: 316 SFGIV 320
>At2g24360.1 68415.m02911 serine/threonine/tyrosine kinase, putative
similar to serine/threonine/tyrosine kinase [Arachis
hypogaea] gi|13124865|gb|AAK11734
Length = 411
Score = 29.9 bits (64), Expect = 2.1
Identities = 16/65 (24%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -2
Query: 201 RSISTDGLLWGAAKLVPLA-YGIHKLQISCV-VEDDKVSIDWLTEEIEKNEDYVQSVDIA 28
R + +D LL A K + +A +G+ ++++ + + + W+ E+ ++ Y Q VD+
Sbjct: 255 RDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMAPEMIQHRAYNQKVDVY 314
Query: 27 AFNKV 13
+F V
Sbjct: 315 SFGIV 319
>At3g19370.1 68416.m02457 expressed protein
Length = 704
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = -2
Query: 591 ELSTKVISLENENKDLKKAIDDLRNLVISLQVRVETLES 475
++ TK+ +L EN LKK++ L +SLQ R +ES
Sbjct: 147 DMKTKIQTLAAENTQLKKSLVAKEELAVSLQERKFQVES 185
>At5g53570.1 68418.m06656 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 550
Score = 29.1 bits (62), Expect = 3.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -2
Query: 540 KAIDDLRNLVISLQVRVETLESSSSI 463
K +DD +LV++L ++ETL S SI
Sbjct: 525 KLLDDAHHLVVTLHDKIETLSQSQSI 550
>At3g58840.1 68416.m06558 expressed protein
Length = 318
Score = 29.1 bits (62), Expect = 3.7
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -2
Query: 606 GVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVETLE 478
GV TEL K+ +EN+N++L + +L+ + L +E ++
Sbjct: 19 GVKTTELERKIEDMENKNQELTRENRELKERLERLTGEIEEMK 61
>At3g57990.1 68416.m06463 expressed protein
Length = 367
Score = 28.7 bits (61), Expect = 4.9
Identities = 10/31 (32%), Positives = 22/31 (70%)
Frame = -2
Query: 591 ELSTKVISLENENKDLKKAIDDLRNLVISLQ 499
+++ + L ENK LK+A++DLR ++ +++
Sbjct: 263 DVAEVIEELRTENKQLKRAVEDLREVISNVR 293
>At2g18260.1 68415.m02129 syntaxin-related protein, putative
(SYP112) similar to SP|Q42374 Syntaxin-related protein
KNOLLE (Syntaxin 111) (AtSYP111) {Arabidopsis thaliana}
Length = 285
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = -2
Query: 651 IKNSLECMDSVATLAGVPNTELSTKVISLENENKDLKKAIDDLRNLVISLQVRVET 484
I S C D V T P +L TK D+K++++ L + + + V VET
Sbjct: 190 ISGSGSCSDLVKTFEVKPEMDLKTK--ERHEAVNDIKRSLNRLHQVFLDMAVLVET 243
>At1g68750.1 68414.m07859 phosphoenolpyruvate carboxylase family
protein / PEP carboxylase family protein similar to
SP|P51059 Phosphoenolpyruvate carboxylase 2 (EC
4.1.1.31) (PEPCASE) {Zea mays}; contains Pfam profile
PF00311: phosphoenolpyruvate carboxylase
Length = 1032
Score = 28.3 bits (60), Expect = 6.4
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -2
Query: 171 GAAKLVPLAYGIHKLQISCVVEDDKVSIDWLTEEIEKNEDYVQSV 37
G ++VPL ++ L+ + +SIDW E I+KN + Q V
Sbjct: 646 GTLRVVPLFETVNDLRAAGPSIRKLLSIDWYREHIQKNHNGHQEV 690
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,008,924
Number of Sequences: 28952
Number of extensions: 269502
Number of successful extensions: 823
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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