BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_pT_B19 (792 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces... 118 1e-27 SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schiz... 57 3e-09 SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schi... 50 3e-07 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 44 3e-05 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 35 0.015 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 35 0.015 SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 30 0.33 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 30 0.44 SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 29 0.76 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 29 0.76 SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharo... 27 2.3 SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos... 27 4.1 SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce... 26 7.1 SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 25 9.4 SPBC1734.11 |||DNAJ domain protein Mas5 |Schizosaccharomyces pom... 25 9.4 SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch... 25 9.4 SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 25 9.4 SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr 2|... 25 9.4 >SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 118 bits (283), Expect = 1e-27 Identities = 59/148 (39%), Positives = 76/148 (51%), Gaps = 13/148 (8%) Frame = -1 Query: 762 YKCNRTGHFARECTQGGVVSRDSGFNRQREKCFKCNRTGHFARDCKE--EADRCYRCNGT 589 Y C GH ARECT+G + C+ CN+TGH A +C E + CY C Sbjct: 20 YNCGENGHQARECTKGSI-------------CYNCNQTGHKASECTEPQQEKTCYACGTA 66 Query: 588 GHIARECAQSPDE---PSCYNCNKTGHIARNCPEGGRES--------ATQTCYNCNKSGH 442 GH+ R+C SP+ CY C + GHIAR+C G++S + CY C GH Sbjct: 67 GHLVRDCPSSPNPRQGAECYKCGRVGHIARDCRTNGQQSGGRFGGHRSNMNCYACGSYGH 126 Query: 441 ISRNCPDGTKTCYVCGKPGHISRECDEA 358 +R+C G K CY CGK GH S EC +A Sbjct: 127 QARDCTMGVK-CYSCGKIGHRSFECQQA 153 Score = 99.5 bits (237), Expect = 5e-22 Identities = 44/119 (36%), Positives = 60/119 (50%), Gaps = 13/119 (10%) Frame = -1 Query: 684 RQREKCFKCNRTGHFARDCKEEADRCYRCNGTGHIARECAQSPDEPSCYNCNKTGHIARN 505 R +C+ C GH AR+C + CY CN TGH A EC + E +CY C GH+ R+ Sbjct: 14 RPGPRCYNCGENGHQARECTK-GSICYNCNQTGHKASECTEPQQEKTCYACGTAGHLVRD 72 Query: 504 CPEGGRESATQTCYNCNKSGHISRNC-PDGTKT------------CYVCGKPGHISREC 367 CP CY C + GHI+R+C +G ++ CY CG GH +R+C Sbjct: 73 CPSSPNPRQGAECYKCGRVGHIARDCRTNGQQSGGRFGGHRSNMNCYACGSYGHQARDC 131 Score = 97.9 bits (233), Expect = 1e-21 Identities = 53/153 (34%), Positives = 72/153 (47%), Gaps = 18/153 (11%) Frame = -1 Query: 771 SVXYKCNRTGHFARECTQGGVVSRDSGFNRQREKCFKCNRTGHFARDC-----KEEADRC 607 S+ Y CN+TGH A ECT+ +Q + C+ C GH RDC + C Sbjct: 36 SICYNCNQTGHKASECTEP----------QQEKTCYACGTAGHLVRDCPSSPNPRQGAEC 85 Query: 606 YRCNGTGHIARECAQSPDEP-----------SCYNCNKTGHIARNCPEGGRESATQTCYN 460 Y+C GHIAR+C + + +CY C GH AR+C G + CY+ Sbjct: 86 YKCGRVGHIARDCRTNGQQSGGRFGGHRSNMNCYACGSYGHQARDCTMGVK------CYS 139 Query: 459 CNKSGHISRNCPDGT--KTCYVCGKPGHISREC 367 C K GH S C + + CY C +PGHI+ C Sbjct: 140 CGKIGHRSFECQQASDGQLCYKCNQPGHIAVNC 172 Score = 74.1 bits (174), Expect = 2e-14 Identities = 31/68 (45%), Positives = 39/68 (57%), Gaps = 2/68 (2%) Frame = -1 Query: 549 PSCYNCNKTGHIARNCPEGGRESATQTCYNCNKSGHISRNC--PDGTKTCYVCGKPGHIS 376 P CYNC + GH AR C +G CYNCN++GH + C P KTCY CG GH+ Sbjct: 17 PRCYNCGENGHQARECTKGS------ICYNCNQTGHKASECTEPQQEKTCYACGTAGHLV 70 Query: 375 RECDEARN 352 R+C + N Sbjct: 71 RDCPSSPN 78 Score = 27.9 bits (59), Expect = 1.8 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = -1 Query: 783 AMSSSVXYKCNRTGHFARECT 721 A + YKCN+ GH A CT Sbjct: 153 ASDGQLCYKCNQPGHIAVNCT 173 >SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 57.2 bits (132), Expect = 3e-09 Identities = 29/99 (29%), Positives = 47/99 (47%), Gaps = 8/99 (8%) Frame = -1 Query: 684 RQREK-CFKCNRTGHFARDCKEEADR---CYRCNGTGHIARECA-QSPDE-PSCYNCNKT 523 R R+K CF C + GH +DC E D C+RC H C+ + P + C+ C++ Sbjct: 73 RNRDKFCFACRQQGHIVQDCPEAKDNVSICFRCGSKEHSLNACSKKGPLKFAKCFICHEN 132 Query: 522 GHIARNCPEG--GRESATQTCYNCNKSGHISRNCPDGTK 412 GH++ C + G C C+ H++++C K Sbjct: 133 GHLSGQCEQNPKGLYPKGGCCKFCSSVHHLAKDCDQVNK 171 Score = 48.4 bits (110), Expect = 1e-06 Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 4/76 (5%) Frame = -1 Query: 576 RECAQSPDEPSCYNCNKTGHIARNCPEGGRESATQTCYNCNKSGHISRNC----PDGTKT 409 R Q + C+ C + GHI ++CPE + C+ C H C P Sbjct: 68 RRINQRNRDKFCFACRQQGHIVQDCPEA--KDNVSICFRCGSKEHSLNACSKKGPLKFAK 125 Query: 408 CYVCGKPGHISRECDE 361 C++C + GH+S +C++ Sbjct: 126 CFICHENGHLSGQCEQ 141 Score = 43.6 bits (98), Expect = 3e-05 Identities = 23/105 (21%), Positives = 49/105 (46%), Gaps = 9/105 (8%) Frame = -1 Query: 762 YKCNRTGHFARECTQGGVVSRDSGFNRQREKCFKCNRTGHFARDCKEEAD----RCYRCN 595 + C + GH ++C + ++D+ CF+C H C ++ +C+ C+ Sbjct: 80 FACRQQGHIVQDCPE----AKDNV-----SICFRCGSKEHSLNACSKKGPLKFAKCFICH 130 Query: 594 GTGHIARECAQSPDE--PS---CYNCNKTGHIARNCPEGGRESAT 475 GH++ +C Q+P P C C+ H+A++C + ++ + Sbjct: 131 ENGHLSGQCEQNPKGLYPKGGCCKFCSSVHHLAKDCDQVNKDDVS 175 Score = 28.7 bits (61), Expect = 1.0 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = -1 Query: 414 KTCYVCGKPGHISRECDEARN 352 K C+ C + GHI ++C EA++ Sbjct: 77 KFCFACRQQGHIVQDCPEAKD 97 >SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schizosaccharomyces pombe|chr 2|||Manual Length = 313 Score = 50.4 bits (115), Expect = 3e-07 Identities = 26/64 (40%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Frame = -1 Query: 543 CYNCNKTGHIARNCPEGGRESATQTCYNCNK-SGHISRNCPDGTKTCYVCGKPGHISREC 367 C+NC GHI+++CP C C HIS CP TK C CG GHI+ C Sbjct: 89 CHNCKGNGHISKDCPH-------VLCTTCGAIDDHISVRCP-WTKKCMNCGLLGHIAARC 140 Query: 366 DEAR 355 E R Sbjct: 141 SEPR 144 Score = 49.6 bits (113), Expect = 5e-07 Identities = 31/102 (30%), Positives = 43/102 (42%), Gaps = 5/102 (4%) Frame = -1 Query: 657 NRTGHFARDCKEEADRCYRCNGTGHIARECAQSPDEPSCYNCNK-TGHIARNCPEGGRES 481 +R +F D E+ C+ C G GHI+++C C C HI+ CP Sbjct: 74 SRGRYFGSD-PSESIVCHNCKGNGHISKDC----PHVLCTTCGAIDDHISVRCP------ 122 Query: 480 ATQTCYNCNKSGHISRNCPD----GTKTCYVCGKPGHISREC 367 T+ C NC GHI+ C + G + C C H S C Sbjct: 123 WTKKCMNCGLLGHIAARCSEPRKRGPRVCRTCHTDTHTSSTC 164 Score = 37.1 bits (82), Expect = 0.003 Identities = 31/130 (23%), Positives = 46/130 (35%), Gaps = 6/130 (4%) Frame = -1 Query: 774 SSVXYKCNRTGHFAREC------TQGGVVSRDSGFNRQREKCFKCNRTGHFARDCKEEAD 613 S V + C GH +++C T G + S +KC C GH A C E Sbjct: 86 SIVCHNCKGNGHISKDCPHVLCTTCGAIDDHISVRCPWTKKCMNCGLLGHIAARCSEPRK 145 Query: 612 RCYRCNGTGHIARECAQSPDEPSCYNCNKTGHIARNCPEGGRESATQTCYNCNKSGHISR 433 R R T H + + Y K + + E + CYNC H Sbjct: 146 RGPRVCRTCHTDTHTSSTCPLIWRYYVEKEHPVRIDVSE-----VRKFCYNCASDEHFGD 200 Query: 432 NCPDGTKTCY 403 +C +++ Y Sbjct: 201 DCTLPSRSNY 210 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 44.0 bits (99), Expect = 3e-05 Identities = 16/40 (40%), Positives = 21/40 (52%) Frame = -1 Query: 543 CYNCNKTGHIARNCPEGGRESATQTCYNCNKSGHISRNCP 424 C NC GH +CPE + C +C GHI+R+CP Sbjct: 311 CQNCGNVGHRRFDCPERINHTMNIVCRHCGSIGHIARDCP 350 Score = 39.5 bits (88), Expect = 5e-04 Identities = 21/56 (37%), Positives = 27/56 (48%), Gaps = 5/56 (8%) Frame = -1 Query: 489 RESATQTCYNCNKSGHISRNCPDGTK-----TCYVCGKPGHISRECDEARN*PQPP 337 R+ Q C NC GH +CP+ C CG GHI+R+C R+ QPP Sbjct: 304 RDDENQVCQNCGNVGHRRFDCPERINHTMNIVCRHCGSIGHIARDC-PVRD-QQPP 357 Score = 36.7 bits (81), Expect = 0.004 Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 5/50 (10%) Frame = -1 Query: 681 QREKCFKCNRTGHFARDCKEEADR-----CYRCNGTGHIARECAQSPDEP 547 + + C C GH DC E + C C GHIAR+C +P Sbjct: 307 ENQVCQNCGNVGHRRFDCPERINHTMNIVCRHCGSIGHIARDCPVRDQQP 356 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 34.7 bits (76), Expect = 0.015 Identities = 13/26 (50%), Positives = 14/26 (53%) Frame = -1 Query: 444 HISRNCPDGTKTCYVCGKPGHISREC 367 H + CP TCY CGK H REC Sbjct: 1794 HTGKACPVRLSTCYKCGKADHNLREC 1819 Score = 27.1 bits (57), Expect = 3.1 Identities = 9/26 (34%), Positives = 11/26 (42%) Frame = -1 Query: 645 HFARDCKEEADRCYRCNGTGHIAREC 568 H + C CY+C H REC Sbjct: 1794 HTGKACPVRLSTCYKCGKADHNLREC 1819 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 34.7 bits (76), Expect = 0.015 Identities = 13/26 (50%), Positives = 14/26 (53%) Frame = -1 Query: 444 HISRNCPDGTKTCYVCGKPGHISREC 367 H + CP TCY CGK H REC Sbjct: 1794 HTGKACPVRLSTCYKCGKADHNLREC 1819 Score = 27.1 bits (57), Expect = 3.1 Identities = 9/26 (34%), Positives = 11/26 (42%) Frame = -1 Query: 645 HFARDCKEEADRCYRCNGTGHIAREC 568 H + C CY+C H REC Sbjct: 1794 HTGKACPVRLSTCYKCGKADHNLREC 1819 >SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 506 Score = 30.3 bits (65), Expect = 0.33 Identities = 14/41 (34%), Positives = 19/41 (46%), Gaps = 4/41 (9%) Frame = -1 Query: 507 NCPEGGRESATQTCYN----CNKSGHISRNCPDGTKTCYVC 397 +CP+G E T C+N C +G+IS P VC Sbjct: 66 DCPDGSDEPGTSACHNGKFFCKNTGYISSYIPSNRVDDTVC 106 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 29.9 bits (64), Expect = 0.44 Identities = 15/58 (25%), Positives = 25/58 (43%), Gaps = 1/58 (1%) Frame = -1 Query: 564 QSPDEPSCYNCNKTGHIARNCPEGGRESATQTCYNCN-KSGHISRNCPDGTKTCYVCG 394 ++ ++P+ N + N + T TC NC ++ + R PDG C CG Sbjct: 603 KNAEKPTVSNAAEDKKGDANTRRANATNPTPTCTNCQTRTTPLWRRSPDGQPLCNACG 660 >SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID complex subunit Taf111|Schizosaccharomyces pombe|chr 1|||Manual Length = 979 Score = 29.1 bits (62), Expect = 0.76 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = -1 Query: 492 GRESATQTCYNCNKSGHISRNCPDGTKTCYVCGKP 388 GR+ T+ C NC + GH+ N K C + G+P Sbjct: 939 GRKPTTRKCSNCGQVGHMKTN-----KICPLFGRP 968 Score = 25.8 bits (54), Expect = 7.1 Identities = 11/28 (39%), Positives = 14/28 (50%), Gaps = 2/28 (7%) Frame = -1 Query: 564 QSPDEPSCYNCNKTGHIARN--CPEGGR 487 + P C NC + GH+ N CP GR Sbjct: 940 RKPTTRKCSNCGQVGHMKTNKICPLFGR 967 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 29.1 bits (62), Expect = 0.76 Identities = 24/108 (22%), Positives = 42/108 (38%), Gaps = 1/108 (0%) Frame = -1 Query: 789 PIAMSSSVXYKCNRTGHFARECTQGGVVSRDSGFNRQREKCFKCNRTGHFAR-DCKEEAD 613 P A +V K N GH E G V +S + K + +R G F+R + Sbjct: 384 PKAAPKAVSQKTNVDGHRNHEAKHGNTVQNESKSQKSSNKEGRSSRGGFFSRLSFSRSSS 443 Query: 612 RCYRCNGTGHIARECAQSPDEPSCYNCNKTGHIARNCPEGGRESATQT 469 R + + H +PD P+ + ++ G+++ T+T Sbjct: 444 RMKKGSKAKH-----EDAPDVPAIPHAYIADSSTKSSYRNGKKTPTRT 486 >SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 27.5 bits (58), Expect = 2.3 Identities = 9/24 (37%), Positives = 17/24 (70%) Frame = -1 Query: 687 NRQREKCFKCNRTGHFARDCKEEA 616 +RQ KC +C RT + +++C+ +A Sbjct: 492 SRQFAKCRRCRRTKYCSKECQHQA 515 >SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizosaccharomyces pombe|chr 2|||Manual Length = 482 Score = 26.6 bits (56), Expect = 4.1 Identities = 13/46 (28%), Positives = 22/46 (47%), Gaps = 4/46 (8%) Frame = -1 Query: 669 CFKCNRTGHFARDCKEEADRCY----RCNGTGHIARECAQSPDEPS 544 C++C + GH+ + C AD Y R T I R ++ + P+ Sbjct: 185 CYRCGQKGHWIQACPTNADPNYDGKPRVKRTTGIPRSFLKNVERPA 230 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/31 (38%), Positives = 15/31 (48%), Gaps = 5/31 (16%) Frame = -1 Query: 576 RECAQSPDEPS-----CYNCNKTGHIARNCP 499 R A PD+P CY C + GH + CP Sbjct: 169 RIAASVPDKPPPPGYICYRCGQKGHWIQACP 199 Score = 26.2 bits (55), Expect = 5.4 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = -1 Query: 468 CYNCNKSGHISRNCPDGTKTCYVCGKP 388 CY C + GH + CP Y GKP Sbjct: 185 CYRCGQKGHWIQACPTNADPNY-DGKP 210 >SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 960 Score = 25.8 bits (54), Expect = 7.1 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +3 Query: 183 YKSTVEPHSSVCGADAALHLYCNRLSCVARGVL 281 Y +T EP ++ GA+ L + R +A+G+L Sbjct: 50 YDNTWEPEQNLFGAEKVLKKWKKRKKLIAKGLL 82 >SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 25.4 bits (53), Expect = 9.4 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = -2 Query: 677 VRSASSATAQDTLRGIARKRLTVA 606 VRSA S+ Q T R +A+ +TVA Sbjct: 50 VRSARSSMEQPTFRPVAKNSVTVA 73 >SPBC1734.11 |||DNAJ domain protein Mas5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 25.4 bits (53), Expect = 9.4 Identities = 8/17 (47%), Positives = 13/17 (76%) Frame = -1 Query: 495 GGRESATQTCYNCNKSG 445 GG+E + ++C +CN SG Sbjct: 144 GGKEGSVKSCASCNGSG 160 >SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 297 Score = 25.4 bits (53), Expect = 9.4 Identities = 16/70 (22%), Positives = 27/70 (38%) Frame = +3 Query: 420 RRDSCGRCGRTCCSYSRSASQTPAPPRDSSVRCGPSCCSCNMTARPGSVRTRALCAQCRY 599 ++DS +CCS + + + P S CCS T+ + ++ Sbjct: 228 KKDSSPSEKPSCCSQEKKSCCSSKKPSCCSQE-KKGCCSTEKTSCCSQEKKSCCTSEKPS 286 Query: 600 ICSNGQPLPC 629 CSNG+ C Sbjct: 287 CCSNGKSTVC 296 >SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase Ade3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 25.4 bits (53), Expect = 9.4 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -1 Query: 426 PDGTKTCYVCGKPGHISRECDEARN 352 PDGT YV +PG IS +A N Sbjct: 64 PDGTTMVYVFPRPGTISPWSSKATN 88 >SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 461 Score = 25.4 bits (53), Expect = 9.4 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +3 Query: 459 SYSRSASQTPAPPRDSSVRCGPSCCSCN 542 +YS + T APP SS G SC +CN Sbjct: 417 TYSPTTLSTNAPPSPSS---GRSCSNCN 441 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,765,665 Number of Sequences: 5004 Number of extensions: 51721 Number of successful extensions: 242 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 191 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 385381248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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