SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_P01
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   399   e-112
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   221   1e-58
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   220   2e-58
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...   161   7e-41
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...   118   8e-28
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...   111   7e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...   100   2e-22
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    66   3e-12
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    57   3e-09
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    34   0.017
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    31   0.16 
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    30   0.28 
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p...    26   4.5  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  399 bits (983), Expect = e-112
 Identities = 181/202 (89%), Positives = 195/202 (96%)
 Frame = +2

Query: 86  DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 265
           ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61

Query: 266 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 445
           RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62  RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121

Query: 446 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDLA 625
           I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYAL HAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181

Query: 626 GRDLTDYLMKILTXRGYSFTTT 691
           GRDLTDYLMKIL  RGY+F+TT
Sbjct: 182 GRDLTDYLMKILMERGYTFSTT 203


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  221 bits (539), Expect = 1e-58
 Identities = 101/201 (50%), Positives = 142/201 (70%), Gaps = 1/201 (0%)
 Frame = +2

Query: 92  DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 271
           D   + +DNGSG  KAGFAGDD P+ +FP+ VGR +H+ VM    QKD +VG EAQ+ RG
Sbjct: 8   DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67

Query: 272 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 448
           +L ++ PIE GII NW DME+IW + + ++ L   PEEHP+LLTE PL    N+EK+ + 
Sbjct: 68  LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127

Query: 449 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDLAG 628
            +ET N PA+  ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++  AI + ++ G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187

Query: 629 RDLTDYLMKILTXRGYSFTTT 691
           RD+TDYL   L   G+   ++
Sbjct: 188 RDVTDYLQLQLRKSGHELVSS 208


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  220 bits (537), Expect = 2e-58
 Identities = 102/200 (51%), Positives = 143/200 (71%), Gaps = 2/200 (1%)
 Frame = +2

Query: 98  AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 271
           A +V+DNG+G  K G+A D+ PR  FPSIVGRP  R +     +  KD  VGDEA++ R 
Sbjct: 4   APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63

Query: 272 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 451
           +L +KYP+E+GII ++++M ++W +TF+ +L++ P    +LLTE P+NP ANREKM + M
Sbjct: 64  LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123

Query: 452 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDLAGR 631
           FE +    +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE   L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183

Query: 632 DLTDYLMKILTXRGYSFTTT 691
           D T YL+ +L  +GY+F  T
Sbjct: 184 DATRYLISLLLRKGYAFNRT 203


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score =  161 bits (392), Expect = 7e-41
 Identities = 90/221 (40%), Positives = 126/221 (57%), Gaps = 32/221 (14%)
 Frame = +2

Query: 104 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 232
           +++DNG+G  K G+AG+DAP  VFP+++   R  G   G         M  K        
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66

Query: 233 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 391
                 D ++G++A  K     +L YPI HG I NWD ME+ W  + +  LR  PE+H  
Sbjct: 67  RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126

Query: 392 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 547
           LLTE PLNP  NRE   +IMFE+FN   +Y+A+QAVL+L AS  +        TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186

Query: 548 GDGVSHTVPIYEGYALXHAILRLDLAGRDLTDYLMKILTXR 670
           GDGV+H +P+ EGY +  +I  + LAGRD+T ++  +L  R
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score =  118 bits (284), Expect = 8e-28
 Identities = 68/222 (30%), Positives = 122/222 (54%), Gaps = 3/222 (1%)
 Frame = +2

Query: 26  AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 205
           A  E + + PT +   F+   +D+  LV+DNGS   +AG+ G+  P+ VF ++V R R +
Sbjct: 5   AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60

Query: 206 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 382
                + +  + VG++   + G  ++ + P E  +I+NWD ME++  +TF  +L +   E
Sbjct: 61  K----LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115

Query: 383 HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDG 556
           HP+ +TE   NP   R  MT+++FE +N+P++   I  + S Y + +  ++GIVL+ G+ 
Sbjct: 116 HPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNA 175

Query: 557 VSHTVPIYEGYALXHAILRLDLAGRDLTDYLMKILTXRGYSF 682
            SH +P+  G  +     R+   G   + YL+K+   +  SF
Sbjct: 176 ASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSF 217


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score =  111 bits (268), Expect = 7e-26
 Identities = 63/196 (32%), Positives = 100/196 (51%)
 Frame = +2

Query: 89  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 268
           D+V+A+V+D GS   + GF+G+D P+ V PS  G       + G    + Y+    +S  
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61

Query: 269 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 448
           G + +K  I +G + NWD    +W +    +L+  P EHP+L+TE   NP  NR K  + 
Sbjct: 62  G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120

Query: 449 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDLAG 628
           MFE+   PA Y+A Q   + +ASG+ T  ++D G   S    IY+G+ L         +G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180

Query: 629 RDLTDYLMKILTXRGY 676
             + D L + L  + +
Sbjct: 181 NAINDILAQTLRDKNF 196


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score =  100 bits (240), Expect = 2e-22
 Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%)
 Frame = +2

Query: 89  DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 268
           +++ +LV+D GS   + G+AG+++P  + PS      + GV   +  ++ YV DE Q   
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61

Query: 269 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 442
            I  + +K    +GII +W+     W      +L+V P E+ +++TE   NP++ R+++ 
Sbjct: 62  PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121

Query: 443 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDL 622
           +  FE  + PA Y+  QAV   +A+ ++T +++D G   +   P+ +G  +   I +  L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181

Query: 623 AGRDLTDYLMKILTXRGYSF 682
           AG  L   + ++       F
Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 39/128 (30%), Positives = 62/128 (48%), Gaps = 4/128 (3%)
 Frame = +2

Query: 290 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 457
           PI+ G + +W+ ++  W H  Y+ L   P +    +PV L         +RE  TQ  FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171

Query: 458 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHAILRLDLAGRDL 637
               P   +A + ++ LYA G   G+V+D G   +   PI +G  +  A  +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231

Query: 638 TDYLMKIL 661
           T +L  +L
Sbjct: 232 TQHLQNLL 239


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 51/210 (24%), Positives = 92/210 (43%), Gaps = 18/210 (8%)
 Frame = +2

Query: 104 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 283
           +V+DNG+   KAGFAG        P+ + R +        G +  ++G+E  +     TL
Sbjct: 8   IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57

Query: 284 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 451
           ++   H  G + +W     +W     N   + P   ++ +LLT+      +      Q++
Sbjct: 58  QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117

Query: 452 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 589
           FE F   A      A L  +  G  T               +V+DSG   +H +P+ +  
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177

Query: 590 ALXHAILRLDLAGRDLTDYLMKILTXRGYS 679
               A+ R+D+ G+ LT+YL ++++ R Y+
Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 27/139 (19%), Positives = 64/139 (46%), Gaps = 12/139 (8%)
 Frame = +2

Query: 281 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 424
           L YPI+ G+    D         D+ +I+ ++  + L++   +   + V+     L  + 
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257

Query: 425 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXHA 604
             EK+  I+F   +     +  +++ + + +G +   V+D G   +    + EG  + ++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317

Query: 605 ILRLDLAGRDLTDYLMKIL 661
            ++++  G D+T   MK+L
Sbjct: 318 RIKINYGGDDITLLFMKLL 336


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1517

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 16/48 (33%), Positives = 26/48 (54%)
 Frame = +2

Query: 149 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 292
           G+  PRA F  ++  P H G+++ M  KD   G+E  S +G  + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1666

 Score = 30.3 bits (65), Expect = 0.28
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
 Frame = +2

Query: 350  FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 493
            +Y  L    E+HP+LLT+  A L P+ +  ++ +I  ++ N+P +    VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451


>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +2

Query: 392 LLTEAPLNPKANREKMTQIMFETFN 466
           +L  APL+ +   +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,638,595
Number of Sequences: 5004
Number of extensions: 52745
Number of successful extensions: 203
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -