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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_O04
         (700 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_25829| Best HMM Match : No HMM Matches (HMM E-Value=.)             181   4e-46
SB_10421| Best HMM Match : Guanylate_cyc (HMM E-Value=0)              141   6e-34
SB_5072| Best HMM Match : Aldedh (HMM E-Value=2.4e-14)                114   7e-26
SB_12802| Best HMM Match : Aldedh (HMM E-Value=0)                      88   7e-18
SB_19772| Best HMM Match : Aldedh (HMM E-Value=5.9e-12)                69   5e-12
SB_58786| Best HMM Match : Aldedh (HMM E-Value=0)                      64   1e-10
SB_37963| Best HMM Match : Aldedh (HMM E-Value=1.8e-16)                56   3e-08
SB_32909| Best HMM Match : No HMM Matches (HMM E-Value=.)              56   3e-08
SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23)                51   1e-06
SB_52935| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.001
SB_13735| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.003
SB_26122| Best HMM Match : Aldedh (HMM E-Value=0)                      30   2.1  
SB_59357| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.7  
SB_39434| Best HMM Match : JmjC (HMM E-Value=0.12)                     28   8.4  
SB_11426| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.4  

>SB_25829| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 826

 Score =  181 bits (441), Expect = 4e-46
 Identities = 85/160 (53%), Positives = 103/160 (64%)
 Frame = +2

Query: 221 LFINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDA 400
           LFINNE+V  + GK F T NP  G+ I ++                  FKLGS WRTMDA
Sbjct: 332 LFINNEFVDCTSGKVFPTINPTTGEKICDISEGDKEDVDKAVKAAKEAFKLGSAWRTMDA 391

Query: 401 SERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHG 580
           S RG L+ KLA LI+RD  YLASLET+D+GK + DS  GD+ +S    RYYAGWADK+ G
Sbjct: 392 SMRGKLLYKLAQLIDRDIAYLASLETIDSGKLFSDS-VGDMQSSANCFRYYAGWADKVTG 450

Query: 581 NVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
             +PADG YF YTRHEP G+ G I PWNFP+ MA+  + P
Sbjct: 451 KTIPADGPYFVYTRHEPVGLVGAITPWNFPLNMASVKIAP 490


>SB_10421| Best HMM Match : Guanylate_cyc (HMM E-Value=0)
          Length = 1485

 Score =  141 bits (341), Expect = 6e-34
 Identities = 66/112 (58%), Positives = 75/112 (66%)
 Frame = +2

Query: 365  FKLGSPWRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNL 544
            FKLGS WRTMDAS RG  + KLADL ERD  YLA LE+ D GK   ++   D+   I   
Sbjct: 1250 FKLGSVWRTMDASARGHFLYKLADLCERDADYLARLESYDGGKVINEAKI-DVQGMIGCF 1308

Query: 545  RYYAGWADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
            RYYAGWADK+ G  +PADG +F YTRHEP GV G I PWNFP+LM    L P
Sbjct: 1309 RYYAGWADKVTGKTIPADGPFFTYTRHEPVGVVGAITPWNFPLLMEGLKLAP 1360


>SB_5072| Best HMM Match : Aldedh (HMM E-Value=2.4e-14)
          Length = 266

 Score =  114 bits (274), Expect = 7e-26
 Identities = 60/164 (36%), Positives = 85/164 (51%), Gaps = 6/164 (3%)
 Frame = +2

Query: 227 INNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASE 406
           I ++W  ++DG+T +  NPA  +VIA V                  F   SPW  +   E
Sbjct: 48  IGDQWCDAADGQTIQVLNPATAEVIARVPRAGQREIDRAVMAARQAFD-DSPWSRIKPVE 106

Query: 407 RGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGNV 586
           R  L+   ADLIE++   LA +E++DNGK    +   D+  ++  LRY AG+A KI G  
Sbjct: 107 RQKLLWNFADLIEKNAALLAEIESIDNGKSAVIAEHVDIRLAVDFLRYMAGFATKIEGRT 166

Query: 587 LPA------DGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
           +        D ++  YTR E  GV G I+ WNFP+L+A W LGP
Sbjct: 167 VQVSVPFMPDAQFHGYTRREAVGVVGAIVAWNFPLLLACWKLGP 210


>SB_12802| Best HMM Match : Aldedh (HMM E-Value=0)
          Length = 880

 Score = 87.8 bits (208), Expect = 7e-18
 Identities = 51/157 (32%), Positives = 74/157 (47%), Gaps = 1/157 (0%)
 Frame = +2

Query: 233 NEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASERG 412
           N   +   G+ F T+ P+ G +  EV+                 FK    W  +  SERG
Sbjct: 283 NPITEEISGQMFITD-PSTGHISCEVKGSGKTEVKRAVLSARKAFKT---WSVLSGSERG 338

Query: 413 ALINKLADLIERDRTYLASLETLDNGKPYKDSYFG-DLYASIKNLRYYAGWADKIHGNVL 589
            ++   + L+ + R  +A +E  DNGKP+ ++ +  D  A +  L YYAG A  + G  +
Sbjct: 339 RILGDASRLVRKRREDIAKVEVHDNGKPFHEALWDVDNVADV--LEYYAGVAPTLSGQHV 396

Query: 590 PADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
                 FAYTR EP GV G I  WN+PI  A W + P
Sbjct: 397 QLPNGSFAYTRREPLGVVGGIGAWNYPIQTACWKIAP 433


>SB_19772| Best HMM Match : Aldedh (HMM E-Value=5.9e-12)
          Length = 211

 Score = 68.5 bits (160), Expect = 5e-12
 Identities = 30/42 (71%), Positives = 33/42 (78%)
 Frame = +2

Query: 467 SLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGNVLP 592
           SLETLDNGKPY DS+  DL  +IK  RYYAGWADKIHG  +P
Sbjct: 32  SLETLDNGKPYNDSFNVDLEFTIKCYRYYAGWADKIHGKTIP 73


>SB_58786| Best HMM Match : Aldedh (HMM E-Value=0)
          Length = 421

 Score = 64.1 bits (149), Expect = 1e-10
 Identities = 34/96 (35%), Positives = 58/96 (60%), Gaps = 2/96 (2%)
 Frame = +2

Query: 398 ASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIH 577
           A ER  ++ K  DLI  +   +A + TL++GKP  ++    LYA+   + +++  A +++
Sbjct: 4   AKERSIILRKWYDLILENIDDIAKIITLESGKPLLEARGEVLYAA-NYIEFFSEEAKRVY 62

Query: 578 GNVLPAD--GKYFAYTRHEPXGVCGQIIPWNFPILM 679
           G+++P++  G+    T+ EP GVC  I PWNFPI M
Sbjct: 63  GDIIPSNNPGQKVLITK-EPVGVCASITPWNFPIAM 97


>SB_37963| Best HMM Match : Aldedh (HMM E-Value=1.8e-16)
          Length = 266

 Score = 56.0 bits (129), Expect = 3e-08
 Identities = 43/162 (26%), Positives = 64/162 (39%), Gaps = 3/162 (1%)
 Frame = +2

Query: 224 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 403
           FIN E++  S  +   + +P+ G+V  +V                  FK    W     +
Sbjct: 14  FINGEFL--SCDRYIDSYDPSRGEVYCKVPDSGKEHVDLAVAAANAAFK---SWSVSSPA 68

Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGW---ADKI 574
            R   + KLADLIE      A  E+ D GK    +   D+     NLR +A         
Sbjct: 69  YRAERLRKLADLIEDRLEEFAQAESRDQGKTVNFARNVDIPRCCLNLRMFASAIMVGSNS 128

Query: 575 HGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
             N L +      Y+   P GV G I PWN P+ + ++ + P
Sbjct: 129 RANFLESQA-VVNYSLRSPKGVAGLISPWNLPLYLLSFKIAP 169


>SB_32909| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1411

 Score = 56.0 bits (129), Expect = 3e-08
 Identities = 29/106 (27%), Positives = 55/106 (51%)
 Frame = +2

Query: 383 WRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGW 562
           W  +    R   +  +A  +++    +A LE++DNGK  +++   D+    ++L ++AGW
Sbjct: 695 WSKLPGHARARHLYSIARHVQKHARLIAVLESMDNGKSVRETRDCDIPNVARHLYHHAGW 754

Query: 563 ADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700
           A  +       D +   +   +P GV G I+PWNFP+++  W + P
Sbjct: 755 AQLM-------DTEMQGW---KPVGVVGGIVPWNFPLMLLCWKVCP 790



 Score = 40.7 bits (91), Expect = 0.001
 Identities = 43/162 (26%), Positives = 63/162 (38%), Gaps = 10/162 (6%)
 Frame = +2

Query: 245  KSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASERGALIN 424
            K  DG   +     +G+VIA+V                   K  S W    A  R  ++ 
Sbjct: 1157 KRPDGNYSRPILNPSGEVIAQV---ADGNRKDIREAVEAAHKAASGWGKRAAHNRAQIVY 1213

Query: 425  KLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN------- 583
             LA+ +E  R  +A+  +   G+   D    ++ ASI+ L YY  +ADK  GN       
Sbjct: 1214 YLAENLEMRRAEVAARISDMTGQSL-DECKAEVDASIQRLFYYGAYADKFGGNRPVFSDK 1272

Query: 584  -VLPADGKYFAYT--RHEPXGVCGQIIPWNFPILMAAWXLGP 700
               P +  ++  T   HEP GV G   P    +L     L P
Sbjct: 1273 DTKPEETPFYGATIKIHEPVGVIGIACPDECSLLAFVSLLAP 1314


>SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23)
          Length = 275

 Score = 50.8 bits (116), Expect = 1e-06
 Identities = 37/160 (23%), Positives = 61/160 (38%), Gaps = 1/160 (0%)
 Frame = +2

Query: 224 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 403
           +I  EWV+ + G      +PA+  +   + H                      W+     
Sbjct: 9   YIAGEWVEGNKGSVANI-SPAD--ISDVIGHYAQADKAQTEAAIAAAAAGQREWQKSGLE 65

Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 583
           +R +++  + D +   +  L ++   + GK   +   G+ Y S +   YYA    +  G 
Sbjct: 66  QRYSVLMAIGDELIARKDELGAILAREEGKTLAEGV-GETYRSGQFFHYYAAEVLRQMGE 124

Query: 584 VLPADGKYFAY-TRHEPXGVCGQIIPWNFPILMAAWXLGP 700
              +        TR EP GV G I PWNFP    AW + P
Sbjct: 125 TAESVRPGIEIETRREPVGVVGIITPWNFPTATGAWKIAP 164


>SB_52935| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1333

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 18/30 (60%), Positives = 22/30 (73%)
 Frame = +2

Query: 221 LFINNEWVKSSDGKTFKTENPANGQVIAEV 310
           LFIN E+V S  GKTF T NP +G V+A+V
Sbjct: 711 LFINGEFVDSHHGKTFNTINPTDGTVLAQV 740


>SB_13735| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 181

 Score = 39.5 bits (88), Expect = 0.003
 Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 1/96 (1%)
 Frame = +2

Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 583
           +RG ++ ++   +    T L  L +L+ GK + +   G++   I    Y  G +    G 
Sbjct: 73  KRGEVVRQIGHALREKLTPLGKLLSLEVGKIFPEGV-GEIQEYIDVCDYAVGLSRMFDGK 131

Query: 584 VLPADGKYFAYTRH-EPXGVCGQIIPWNFPILMAAW 688
           V P++    A      P G  G I  +NFP+ +  W
Sbjct: 132 VFPSERPGHALLEQWNPVGTVGVITAFNFPVAVFGW 167


>SB_26122| Best HMM Match : Aldedh (HMM E-Value=0)
          Length = 462

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 26/98 (26%), Positives = 38/98 (38%), Gaps = 1/98 (1%)
 Frame = +2

Query: 374 GSPWRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYAS-IKNLRY 550
           GS +R     E   L     DL+E+     +  ETLD G     +     +A+ + N+R 
Sbjct: 66  GSSYREAKVIESLELPRNYRDLLEKRSEDFSKAETLDTGTVISRANNNKEFANEVLNIRV 125

Query: 551 YAGWADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWN 664
               A  I G    A+G    Y+     GV   I  W+
Sbjct: 126 LVDHALSIMGE-STAEGGTLCYSIRHSLGVVAIITSWS 162


>SB_59357| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1716

 Score = 29.5 bits (63), Expect = 2.7
 Identities = 18/67 (26%), Positives = 26/67 (38%)
 Frame = -1

Query: 421  DKGPPLGGVHCSPW*SKFERISSCLHSFVHICFVGMLDLSYNLAIGRVFSFEGLPIRRLH 242
            D  P +  V C  +      I  CLHSF   C V  L  SY+  +      +  P   + 
Sbjct: 1359 DLNPHIICVLCGGYLVDATTIVECLHSFCRSCIVSWLQASYHCPVCDTEVHKTRPFLNIR 1418

Query: 241  PLIVYEE 221
            P  V ++
Sbjct: 1419 PDCVLQD 1425


>SB_39434| Best HMM Match : JmjC (HMM E-Value=0.12)
          Length = 672

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 18/71 (25%), Positives = 30/71 (42%), Gaps = 2/71 (2%)
 Frame = +2

Query: 425 KLADLIERDRTYLASLETLDNGKPYKD--SYFGDLYASIKNLRYYAGWADKIHGNVLPAD 598
           K  DL+E   + L  +  +     Y    SYF +L   I+ +   AG  ++ H N+  +D
Sbjct: 404 KFMDLLEGRNSNLTKMSNISAYLEYTSIPSYFPELKQDIQEMPCAAGLLNRKHLNIWLSD 463

Query: 599 GKYFAYTRHEP 631
           G        +P
Sbjct: 464 GNTLGKLHFDP 474


>SB_11426| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 522

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
 Frame = +1

Query: 97  YKYIKNVAERLQIGSGAKSEFCNSCFSGTGSPNKTGNFI---HRSLHK 231
           +KY K++ + +++    K   CN C     S +K  N +   HR  HK
Sbjct: 166 FKYAKSLKQHIEVHCDPKRVKCNYCCIDLPSTSKLANRVVKRHRGEHK 213


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,694,897
Number of Sequences: 59808
Number of extensions: 454316
Number of successful extensions: 1066
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 975
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1056
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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