BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_O04 (700 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_25829| Best HMM Match : No HMM Matches (HMM E-Value=.) 181 4e-46 SB_10421| Best HMM Match : Guanylate_cyc (HMM E-Value=0) 141 6e-34 SB_5072| Best HMM Match : Aldedh (HMM E-Value=2.4e-14) 114 7e-26 SB_12802| Best HMM Match : Aldedh (HMM E-Value=0) 88 7e-18 SB_19772| Best HMM Match : Aldedh (HMM E-Value=5.9e-12) 69 5e-12 SB_58786| Best HMM Match : Aldedh (HMM E-Value=0) 64 1e-10 SB_37963| Best HMM Match : Aldedh (HMM E-Value=1.8e-16) 56 3e-08 SB_32909| Best HMM Match : No HMM Matches (HMM E-Value=.) 56 3e-08 SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23) 51 1e-06 SB_52935| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.001 SB_13735| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.003 SB_26122| Best HMM Match : Aldedh (HMM E-Value=0) 30 2.1 SB_59357| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.7 SB_39434| Best HMM Match : JmjC (HMM E-Value=0.12) 28 8.4 SB_11426| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.4 >SB_25829| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 826 Score = 181 bits (441), Expect = 4e-46 Identities = 85/160 (53%), Positives = 103/160 (64%) Frame = +2 Query: 221 LFINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDA 400 LFINNE+V + GK F T NP G+ I ++ FKLGS WRTMDA Sbjct: 332 LFINNEFVDCTSGKVFPTINPTTGEKICDISEGDKEDVDKAVKAAKEAFKLGSAWRTMDA 391 Query: 401 SERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHG 580 S RG L+ KLA LI+RD YLASLET+D+GK + DS GD+ +S RYYAGWADK+ G Sbjct: 392 SMRGKLLYKLAQLIDRDIAYLASLETIDSGKLFSDS-VGDMQSSANCFRYYAGWADKVTG 450 Query: 581 NVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 +PADG YF YTRHEP G+ G I PWNFP+ MA+ + P Sbjct: 451 KTIPADGPYFVYTRHEPVGLVGAITPWNFPLNMASVKIAP 490 >SB_10421| Best HMM Match : Guanylate_cyc (HMM E-Value=0) Length = 1485 Score = 141 bits (341), Expect = 6e-34 Identities = 66/112 (58%), Positives = 75/112 (66%) Frame = +2 Query: 365 FKLGSPWRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNL 544 FKLGS WRTMDAS RG + KLADL ERD YLA LE+ D GK ++ D+ I Sbjct: 1250 FKLGSVWRTMDASARGHFLYKLADLCERDADYLARLESYDGGKVINEAKI-DVQGMIGCF 1308 Query: 545 RYYAGWADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 RYYAGWADK+ G +PADG +F YTRHEP GV G I PWNFP+LM L P Sbjct: 1309 RYYAGWADKVTGKTIPADGPFFTYTRHEPVGVVGAITPWNFPLLMEGLKLAP 1360 >SB_5072| Best HMM Match : Aldedh (HMM E-Value=2.4e-14) Length = 266 Score = 114 bits (274), Expect = 7e-26 Identities = 60/164 (36%), Positives = 85/164 (51%), Gaps = 6/164 (3%) Frame = +2 Query: 227 INNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASE 406 I ++W ++DG+T + NPA +VIA V F SPW + E Sbjct: 48 IGDQWCDAADGQTIQVLNPATAEVIARVPRAGQREIDRAVMAARQAFD-DSPWSRIKPVE 106 Query: 407 RGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGNV 586 R L+ ADLIE++ LA +E++DNGK + D+ ++ LRY AG+A KI G Sbjct: 107 RQKLLWNFADLIEKNAALLAEIESIDNGKSAVIAEHVDIRLAVDFLRYMAGFATKIEGRT 166 Query: 587 LPA------DGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 + D ++ YTR E GV G I+ WNFP+L+A W LGP Sbjct: 167 VQVSVPFMPDAQFHGYTRREAVGVVGAIVAWNFPLLLACWKLGP 210 >SB_12802| Best HMM Match : Aldedh (HMM E-Value=0) Length = 880 Score = 87.8 bits (208), Expect = 7e-18 Identities = 51/157 (32%), Positives = 74/157 (47%), Gaps = 1/157 (0%) Frame = +2 Query: 233 NEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASERG 412 N + G+ F T+ P+ G + EV+ FK W + SERG Sbjct: 283 NPITEEISGQMFITD-PSTGHISCEVKGSGKTEVKRAVLSARKAFKT---WSVLSGSERG 338 Query: 413 ALINKLADLIERDRTYLASLETLDNGKPYKDSYFG-DLYASIKNLRYYAGWADKIHGNVL 589 ++ + L+ + R +A +E DNGKP+ ++ + D A + L YYAG A + G + Sbjct: 339 RILGDASRLVRKRREDIAKVEVHDNGKPFHEALWDVDNVADV--LEYYAGVAPTLSGQHV 396 Query: 590 PADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 FAYTR EP GV G I WN+PI A W + P Sbjct: 397 QLPNGSFAYTRREPLGVVGGIGAWNYPIQTACWKIAP 433 >SB_19772| Best HMM Match : Aldedh (HMM E-Value=5.9e-12) Length = 211 Score = 68.5 bits (160), Expect = 5e-12 Identities = 30/42 (71%), Positives = 33/42 (78%) Frame = +2 Query: 467 SLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGNVLP 592 SLETLDNGKPY DS+ DL +IK RYYAGWADKIHG +P Sbjct: 32 SLETLDNGKPYNDSFNVDLEFTIKCYRYYAGWADKIHGKTIP 73 >SB_58786| Best HMM Match : Aldedh (HMM E-Value=0) Length = 421 Score = 64.1 bits (149), Expect = 1e-10 Identities = 34/96 (35%), Positives = 58/96 (60%), Gaps = 2/96 (2%) Frame = +2 Query: 398 ASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIH 577 A ER ++ K DLI + +A + TL++GKP ++ LYA+ + +++ A +++ Sbjct: 4 AKERSIILRKWYDLILENIDDIAKIITLESGKPLLEARGEVLYAA-NYIEFFSEEAKRVY 62 Query: 578 GNVLPAD--GKYFAYTRHEPXGVCGQIIPWNFPILM 679 G+++P++ G+ T+ EP GVC I PWNFPI M Sbjct: 63 GDIIPSNNPGQKVLITK-EPVGVCASITPWNFPIAM 97 >SB_37963| Best HMM Match : Aldedh (HMM E-Value=1.8e-16) Length = 266 Score = 56.0 bits (129), Expect = 3e-08 Identities = 43/162 (26%), Positives = 64/162 (39%), Gaps = 3/162 (1%) Frame = +2 Query: 224 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 403 FIN E++ S + + +P+ G+V +V FK W + Sbjct: 14 FINGEFL--SCDRYIDSYDPSRGEVYCKVPDSGKEHVDLAVAAANAAFK---SWSVSSPA 68 Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGW---ADKI 574 R + KLADLIE A E+ D GK + D+ NLR +A Sbjct: 69 YRAERLRKLADLIEDRLEEFAQAESRDQGKTVNFARNVDIPRCCLNLRMFASAIMVGSNS 128 Query: 575 HGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 N L + Y+ P GV G I PWN P+ + ++ + P Sbjct: 129 RANFLESQA-VVNYSLRSPKGVAGLISPWNLPLYLLSFKIAP 169 >SB_32909| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1411 Score = 56.0 bits (129), Expect = 3e-08 Identities = 29/106 (27%), Positives = 55/106 (51%) Frame = +2 Query: 383 WRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGW 562 W + R + +A +++ +A LE++DNGK +++ D+ ++L ++AGW Sbjct: 695 WSKLPGHARARHLYSIARHVQKHARLIAVLESMDNGKSVRETRDCDIPNVARHLYHHAGW 754 Query: 563 ADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWNFPILMAAWXLGP 700 A + D + + +P GV G I+PWNFP+++ W + P Sbjct: 755 AQLM-------DTEMQGW---KPVGVVGGIVPWNFPLMLLCWKVCP 790 Score = 40.7 bits (91), Expect = 0.001 Identities = 43/162 (26%), Positives = 63/162 (38%), Gaps = 10/162 (6%) Frame = +2 Query: 245 KSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDASERGALIN 424 K DG + +G+VIA+V K S W A R ++ Sbjct: 1157 KRPDGNYSRPILNPSGEVIAQV---ADGNRKDIREAVEAAHKAASGWGKRAAHNRAQIVY 1213 Query: 425 KLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN------- 583 LA+ +E R +A+ + G+ D ++ ASI+ L YY +ADK GN Sbjct: 1214 YLAENLEMRRAEVAARISDMTGQSL-DECKAEVDASIQRLFYYGAYADKFGGNRPVFSDK 1272 Query: 584 -VLPADGKYFAYT--RHEPXGVCGQIIPWNFPILMAAWXLGP 700 P + ++ T HEP GV G P +L L P Sbjct: 1273 DTKPEETPFYGATIKIHEPVGVIGIACPDECSLLAFVSLLAP 1314 >SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23) Length = 275 Score = 50.8 bits (116), Expect = 1e-06 Identities = 37/160 (23%), Positives = 61/160 (38%), Gaps = 1/160 (0%) Frame = +2 Query: 224 FINNEWVKSSDGKTFKTENPANGQVIAEVQHXXXXXXXXXXXXXXXXFKLGSPWRTMDAS 403 +I EWV+ + G +PA+ + + H W+ Sbjct: 9 YIAGEWVEGNKGSVANI-SPAD--ISDVIGHYAQADKAQTEAAIAAAAAGQREWQKSGLE 65 Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 583 +R +++ + D + + L ++ + GK + G+ Y S + YYA + G Sbjct: 66 QRYSVLMAIGDELIARKDELGAILAREEGKTLAEGV-GETYRSGQFFHYYAAEVLRQMGE 124 Query: 584 VLPADGKYFAY-TRHEPXGVCGQIIPWNFPILMAAWXLGP 700 + TR EP GV G I PWNFP AW + P Sbjct: 125 TAESVRPGIEIETRREPVGVVGIITPWNFPTATGAWKIAP 164 >SB_52935| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1333 Score = 40.3 bits (90), Expect = 0.001 Identities = 18/30 (60%), Positives = 22/30 (73%) Frame = +2 Query: 221 LFINNEWVKSSDGKTFKTENPANGQVIAEV 310 LFIN E+V S GKTF T NP +G V+A+V Sbjct: 711 LFINGEFVDSHHGKTFNTINPTDGTVLAQV 740 >SB_13735| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 181 Score = 39.5 bits (88), Expect = 0.003 Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 1/96 (1%) Frame = +2 Query: 404 ERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYASIKNLRYYAGWADKIHGN 583 +RG ++ ++ + T L L +L+ GK + + G++ I Y G + G Sbjct: 73 KRGEVVRQIGHALREKLTPLGKLLSLEVGKIFPEGV-GEIQEYIDVCDYAVGLSRMFDGK 131 Query: 584 VLPADGKYFAYTRH-EPXGVCGQIIPWNFPILMAAW 688 V P++ A P G G I +NFP+ + W Sbjct: 132 VFPSERPGHALLEQWNPVGTVGVITAFNFPVAVFGW 167 >SB_26122| Best HMM Match : Aldedh (HMM E-Value=0) Length = 462 Score = 29.9 bits (64), Expect = 2.1 Identities = 26/98 (26%), Positives = 38/98 (38%), Gaps = 1/98 (1%) Frame = +2 Query: 374 GSPWRTMDASERGALINKLADLIERDRTYLASLETLDNGKPYKDSYFGDLYAS-IKNLRY 550 GS +R E L DL+E+ + ETLD G + +A+ + N+R Sbjct: 66 GSSYREAKVIESLELPRNYRDLLEKRSEDFSKAETLDTGTVISRANNNKEFANEVLNIRV 125 Query: 551 YAGWADKIHGNVLPADGKYFAYTRHEPXGVCGQIIPWN 664 A I G A+G Y+ GV I W+ Sbjct: 126 LVDHALSIMGE-STAEGGTLCYSIRHSLGVVAIITSWS 162 >SB_59357| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1716 Score = 29.5 bits (63), Expect = 2.7 Identities = 18/67 (26%), Positives = 26/67 (38%) Frame = -1 Query: 421 DKGPPLGGVHCSPW*SKFERISSCLHSFVHICFVGMLDLSYNLAIGRVFSFEGLPIRRLH 242 D P + V C + I CLHSF C V L SY+ + + P + Sbjct: 1359 DLNPHIICVLCGGYLVDATTIVECLHSFCRSCIVSWLQASYHCPVCDTEVHKTRPFLNIR 1418 Query: 241 PLIVYEE 221 P V ++ Sbjct: 1419 PDCVLQD 1425 >SB_39434| Best HMM Match : JmjC (HMM E-Value=0.12) Length = 672 Score = 27.9 bits (59), Expect = 8.4 Identities = 18/71 (25%), Positives = 30/71 (42%), Gaps = 2/71 (2%) Frame = +2 Query: 425 KLADLIERDRTYLASLETLDNGKPYKD--SYFGDLYASIKNLRYYAGWADKIHGNVLPAD 598 K DL+E + L + + Y SYF +L I+ + AG ++ H N+ +D Sbjct: 404 KFMDLLEGRNSNLTKMSNISAYLEYTSIPSYFPELKQDIQEMPCAAGLLNRKHLNIWLSD 463 Query: 599 GKYFAYTRHEP 631 G +P Sbjct: 464 GNTLGKLHFDP 474 >SB_11426| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 522 Score = 27.9 bits (59), Expect = 8.4 Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = +1 Query: 97 YKYIKNVAERLQIGSGAKSEFCNSCFSGTGSPNKTGNFI---HRSLHK 231 +KY K++ + +++ K CN C S +K N + HR HK Sbjct: 166 FKYAKSLKQHIEVHCDPKRVKCNYCCIDLPSTSKLANRVVKRHRGEHK 213 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,694,897 Number of Sequences: 59808 Number of extensions: 454316 Number of successful extensions: 1066 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 975 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1056 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1829596184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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