Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_O02
         (708 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protei...   121   4e-28
At5g28140.1 68418.m03398 hypothetical protein                          29   4.0  
At1g63520.1 68414.m07181 expressed protein                             28   7.0  
At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) / c...    27   9.2  
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil...    27   9.2  

>At4g02620.1 68417.m00356 vacuolar ATPase subunit F family protein
           contains weak similarity to vacuolar ATP synthase
           subunit F (EC 3.6.3.14) (V-ATPase F subunit) (Vacuolar
           proton pump F subunit) (V-ATPase 14 kDa subunit)
           (Swiss-Prot:P50408) [Rattus norvegicus]; contains Pfam
           PF01990: ATP synthase (F/14-kDa) subunit
          Length = 128

 Score =  121 bits (292), Expect = 4e-28
 Identities = 52/112 (46%), Positives = 79/112 (70%)
 Frame = +3

Query: 81  LISVIGDEDTCVGFLLGGIGEINKNRHPNFMVVDKNTPVSEIEECFKRFVKRXXXXXXLI 260
           LI++I DEDT VGFL+ G+G ++  R  N+++VD  T V +IE+ FK F  R      L+
Sbjct: 14  LIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTTVRQIEDAFKEFSARDDIAIILL 73

Query: 261 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYDASKDSILRRAKGMFNPDDL 416
           +Q IA +IR ++D+++ PVP++LEIPSKDHPYD + DS+L R K +F+ + +
Sbjct: 74  SQYIANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFSAESV 125


>At5g28140.1 68418.m03398 hypothetical protein 
          Length = 212

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = +3

Query: 297 DAHSAPVPSVLEIPSKDHPYDASK 368
           DAHS P+ ++ EI S+ HP  A K
Sbjct: 137 DAHSRPMTNISEIASEKHPEPALK 160


>At1g63520.1 68414.m07181 expressed protein
          Length = 528

 Score = 27.9 bits (59), Expect = 7.0
 Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 2/77 (2%)
 Frame = -2

Query: 302 RINHMSDQFSN--VLIDENDVNVIAFNKTLETFLDFTHRCILIDHHEIRMSVFIDLTDAP 129
           RI  MS+ FSN   L+ E+   + + NK+    L   H  + +++ E+ M VFI   D P
Sbjct: 150 RIKKMSNPFSNRNPLLPESGQEMFSRNKSSPVHL---HAHLRMEY-ELGMPVFIFSLDLP 205

Query: 128 E*ESDASVLIPDNTNQF 78
           +    AS  + D  ++F
Sbjct: 206 DDVYMASTRMDDKESRF 222


>At5g25900.1 68418.m03075 ent-kaurene oxidase, putative (GA3) /
           cytochrome P450 identical to GA3 [Arabidopsis thaliana]
           GI:3342249; similar to ent-kaurene oxidase [Cucurbita
           maxima] GI:11934675; contains Pfam profile PF00067:
           Cytochrome P450
          Length = 509

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 3/93 (3%)
 Frame = +3

Query: 90  VIGDEDTCVGFLLGGIGEINKNRHPNFMVV---DKNTPVSEIEECFKRFVKRXXXXXXLI 260
           V+   +T    ++     I+  +  N + V   DK+   +   + F + VKR      L+
Sbjct: 90  VLNSTETAKEAMVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNG-LL 148

Query: 261 NQNIAELIRHVIDAHSAPVPSVLEIPSKDHPYD 359
             N  +  RH  DA    V S L   ++DHP +
Sbjct: 149 GANAQKRKRHYRDALIENVSSKLHAHARDHPQE 181


>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
           LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
           HMM hit: AMP-binding enzymes PF00501
          Length = 691

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
 Frame = -2

Query: 251 DVNVIAFNKTLETFLDFTHRCILIDHH-EIRMSVFID 144
           D   + F KTLE   DF    + I H  E R+++F D
Sbjct: 103 DYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFAD 139


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,434,943
Number of Sequences: 28952
Number of extensions: 302000
Number of successful extensions: 811
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 811
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)