Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_N20
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 1.8
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 2.4
SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |... 26 4.2
SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 26 4.2
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 26 5.5
SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 7.3
SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz... 25 7.3
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 25 9.7
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 27.5 bits (58), Expect = 1.8
Identities = 19/83 (22%), Positives = 38/83 (45%), Gaps = 1/83 (1%)
Frame = +1
Query: 256 GYETDNGISAQSSGSLKKVDNIDVLAIQGQYEYS-APDGTPVKFTYTADENGYQPQSELL 432
G+ + +A S+ + +++V A+ A +G+ V T D N S +
Sbjct: 1089 GHSRNGSHAAHSNNVIGTQPHVNVSAVPNTGNLKDALEGSAVSKTDDVD-NVVSGHSNVN 1147
Query: 433 PVAPPMPEAIRRAIDYILAHPPK 501
V+ P + R+ DY+++H P+
Sbjct: 1148 GVSKSRPNIVERSEDYVISHLPE 1170
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +1
Query: 349 EYSAPDGTPVKFTYTADENGYQPQ-SELLPVAPPMP 453
+Y + V+ T T DE+G+Q Q + VA P+P
Sbjct: 955 DYEGTEDNIVRQTTTVDEDGHQEQTTSTTKVAHPIP 990
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 27.1 bits (57), Expect = 2.4
Identities = 32/127 (25%), Positives = 53/127 (41%), Gaps = 10/127 (7%)
Frame = +1
Query: 232 VNPDGFSFGYETDNGISAQSSGSLK--KVDNIDVLAIQGQYEYSA----PDGTPVKFTY- 390
++ D S + + + S SL K+ ++D+ + Y SA P+ + + F Y
Sbjct: 634 ISSDSESLSQSLNMAVDFRQSSSLPADKLSSLDI-HFEDDYNSSADEEDPEASDISFQYS 692
Query: 391 -TADENGYQPQSELLPVAPPMP--EAIRRAIDYILAHPPKTETVKKL*IQKIQSTTTPAK 561
T+D+ G + + A + E A YI + E +KL +Q I P
Sbjct: 693 NTSDKEGSSDKDSSIEEASSVKTQENGLNATLYI-----QMEYCEKLSLQDIIRDKIPVD 747
Query: 562 EKWTLFR 582
E W LFR
Sbjct: 748 EMWRLFR 754
>SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 777
Score = 26.2 bits (55), Expect = 4.2
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 13/100 (13%)
Frame = +2
Query: 239 LTDSALGMKLTMESALNHLDP*RKLIISMFSPSKV----NMNTLRLTEPL-*SSRIQLTR 403
L +S L + ALNH K ++S ++PS V + L L + + SS +L R
Sbjct: 496 LVNSPLSSVHQLREALNHSSSVTKEVLSQYTPSVVIGVLKLYFLELPDSIVPSSAFELIR 555
Query: 404 T-------DTNHRVSYFQ-LLLQCLKPSVALLTTFSLTHL 499
+ DT +R+ Q LL Q + ++A L+ +THL
Sbjct: 556 SIYMNHSNDTPYRLRLLQNLLSQLRRVNLATLSAI-ITHL 594
>SPAC3C7.12 |tip1|noc1|CLIP170 family protein
Tip1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 461
Score = 26.2 bits (55), Expect = 4.2
Identities = 17/64 (26%), Positives = 32/64 (50%)
Frame = +2
Query: 272 MESALNHLDP*RKLIISMFSPSKVNMNTLRLTEPL*SSRIQLTRTDTNHRVSYFQLLLQC 451
+E L +D + L+ + F+ + ++ LR L RIQ R + + R F+ ++C
Sbjct: 156 LEEVLATVDQLQSLVTN-FNDQQDEVDELRERITLKEERIQQMRNEASQRRFEFKTTIEC 214
Query: 452 LKPS 463
L+ S
Sbjct: 215 LEES 218
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 25.8 bits (54), Expect = 5.5
Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = -2
Query: 381 LHRGSVRRRVFILTLDGENIDIINFLQG-SR*LSAD 277
L G V+ + F+L +D E IDI+ + G R +S D
Sbjct: 970 LRSGLVKHKAFVLAVDQEYIDIVIYEFGLERRISLD 1005
>SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 463
Score = 25.4 bits (53), Expect = 7.3
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +1
Query: 208 VILRSDTEVNPDGFSFGYETDNGISAQSSGS 300
++ +SDT GFS G +G S + +G+
Sbjct: 99 IVFKSDTNSTSSGFSVGEAISSGYSLKYNGT 129
>SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 451
Score = 25.4 bits (53), Expect = 7.3
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 190 GSEADAVILRSDTEVNPDGFSFGYETDNGIS 282
G +A V + T DGF +GY T+ GIS
Sbjct: 365 GIDASGVYWNASTRF-ADGFRYGYGTEVGIS 394
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 25.0 bits (52), Expect = 9.7
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 503 PKP*KSYKSRRFRAQRPPPKKSGLYFEHHTLSISI 607
PKP KS ++ F PPP +G H L+I+I
Sbjct: 50 PKP-KSIEAPMFPILLPPPNITGKLHIGHALTITI 83
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,472,084
Number of Sequences: 5004
Number of extensions: 45149
Number of successful extensions: 115
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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