BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_N19 (611 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 1.6 SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |... 26 3.7 SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 26 3.7 SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 26 4.9 SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch... 25 6.5 SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schiz... 25 6.5 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 6.5 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 27.5 bits (58), Expect = 1.6 Identities = 19/83 (22%), Positives = 38/83 (45%), Gaps = 1/83 (1%) Frame = +3 Query: 255 GYETDNGISAQSSGSLKKVDNIDVLAIQGQYEYS-APDGTPVKFTYTADENGYQPQSELL 431 G+ + +A S+ + +++V A+ A +G+ V T D N S + Sbjct: 1089 GHSRNGSHAAHSNNVIGTQPHVNVSAVPNTGNLKDALEGSAVSKTDDVD-NVVSGHSNVN 1147 Query: 432 PVAPPMPEAIRRAIDYILAHPPK 500 V+ P + R+ DY+++H P+ Sbjct: 1148 GVSKSRPNIVERSEDYVISHLPE 1170 Score = 25.8 bits (54), Expect = 4.9 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Frame = +3 Query: 348 EYSAPDGTPVKFTYTADENGYQPQ-SELLPVAPPMP 452 +Y + V+ T T DE+G+Q Q + VA P+P Sbjct: 955 DYEGTEDNIVRQTTTVDEDGHQEQTTSTTKVAHPIP 990 >SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 777 Score = 26.2 bits (55), Expect = 3.7 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 13/100 (13%) Frame = +1 Query: 238 LTDSALGMKLTMESALNHLDP*RKLIISMFSPSKV----NMNTLRLTEPL-*SSRIQLTR 402 L +S L + ALNH K ++S ++PS V + L L + + SS +L R Sbjct: 496 LVNSPLSSVHQLREALNHSSSVTKEVLSQYTPSVVIGVLKLYFLELPDSIVPSSAFELIR 555 Query: 403 T-------DTNHRVSYFQ-LLLQCLKPSVALLTTFSLTHL 498 + DT +R+ Q LL Q + ++A L+ +THL Sbjct: 556 SIYMNHSNDTPYRLRLLQNLLSQLRRVNLATLSAI-ITHL 594 >SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 26.2 bits (55), Expect = 3.7 Identities = 17/64 (26%), Positives = 32/64 (50%) Frame = +1 Query: 271 MESALNHLDP*RKLIISMFSPSKVNMNTLRLTEPL*SSRIQLTRTDTNHRVSYFQLLLQC 450 +E L +D + L+ + F+ + ++ LR L RIQ R + + R F+ ++C Sbjct: 156 LEEVLATVDQLQSLVTN-FNDQQDEVDELRERITLKEERIQQMRNEASQRRFEFKTTIEC 214 Query: 451 LKPS 462 L+ S Sbjct: 215 LEES 218 >SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2|||Manual Length = 1157 Score = 25.8 bits (54), Expect = 4.9 Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = -1 Query: 380 LHRGSVRRRVFILTLDGENIDIINFLQG-SR*LSAD 276 L G V+ + F+L +D E IDI+ + G R +S D Sbjct: 970 LRSGLVKHKAFVLAVDQEYIDIVIYEFGLERRISLD 1005 >SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 463 Score = 25.4 bits (53), Expect = 6.5 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = +3 Query: 207 VILRSDTEVNPDGFSFGYETDNGISAQSSGS 299 ++ +SDT GFS G +G S + +G+ Sbjct: 99 IVFKSDTNSTSSGFSVGEAISSGYSLKYNGT 129 >SPAC821.11 |pro1||gamma-glutamyl phosphate reductase Pro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 451 Score = 25.4 bits (53), Expect = 6.5 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = +3 Query: 189 GSEADAVILRSDTEVNPDGFSFGYETDNGIS 281 G +A V + T DGF +GY T+ GIS Sbjct: 365 GIDASGVYWNASTRF-ADGFRYGYGTEVGIS 394 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 25.4 bits (53), Expect = 6.5 Identities = 31/127 (24%), Positives = 52/127 (40%), Gaps = 10/127 (7%) Frame = +3 Query: 231 VNPDGFSFGYETDNGISAQSSGSLK--KVDNIDVLAIQGQYEYSA----PDGTPVKFTY- 389 ++ D S + + + S SL K+ ++D+ + Y SA P+ + + F Y Sbjct: 634 ISSDSESLSQSLNMAVDFRQSSSLPADKLSSLDI-HFEDDYNSSADEEDPEASDISFQYS 692 Query: 390 -TADENGYQPQSELLPVAPPMP--EAIRRAIDYILAHPPKTETVKKL*IXKIXSTTTPAK 560 T+D+ G + + A + E A YI + E +KL + I P Sbjct: 693 NTSDKEGSSDKDSSIEEASSVKTQENGLNATLYI-----QMEYCEKLSLQDIIRDKIPVD 747 Query: 561 EKWTLFR 581 E W LFR Sbjct: 748 EMWRLFR 754 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,263,847 Number of Sequences: 5004 Number of extensions: 39759 Number of successful extensions: 99 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 98 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 99 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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