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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_N16
         (605 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei...   196   3e-51
SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc...   155   6e-39
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    48   1e-06
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p...    33   0.043
SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyce...    26   3.7  
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|...    26   4.9  

>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
           Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 759

 Score =  196 bits (477), Expect = 3e-51
 Identities = 83/143 (58%), Positives = 107/143 (74%)
 Frame = +1

Query: 175 QQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRA 354
           ++ E+RDG+RFTWNV+PS+RIE++R +VP+A +Y+PL ERPDLPP+ YEPV C +  C+A
Sbjct: 4   EEIEERDGVRFTWNVFPSTRIESSRTIVPIASIYKPLNERPDLPPVLYEPVTC-KAPCKA 62

Query: 355 ILNPMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMP 534
           +LNP C +D RAK W+C FC QRN  PPQY  IS    P EL+P +STIEYT+ R   + 
Sbjct: 63  VLNPYCHIDTRAKFWICPFCLQRNMLPPQYKDISNTSLPIELLPEYSTIEYTLPRPPQLT 122

Query: 535 PIYLLVVDTCLXEEELGALKDSL 603
           P++L VVD C  EE L ALKDSL
Sbjct: 123 PVFLFVVDVCQDEENLQALKDSL 145


>SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 765

 Score =  155 bits (375), Expect = 6e-39
 Identities = 67/140 (47%), Positives = 93/140 (66%)
 Frame = +1

Query: 184 EDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRAILN 363
           ED+DGIR +WN + ++  E  R V+P+A +Y PL E   +   QY+PV C R  CRA+LN
Sbjct: 7   EDQDGIRLSWNTFSATPAENARAVIPIAAMYTPLHENERMTIEQYDPVAC-RAPCRAVLN 65

Query: 364 PMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIY 543
           P C VD RA+ W+C FCFQRNP P QY+ IS    P EL+   +T+EY +++    PP++
Sbjct: 66  PYCHVDLRARFWICPFCFQRNPLPAQYSDISSNSLPLELLSQSTTMEYVLSKPVKSPPVF 125

Query: 544 LLVVDTCLXEEELGALKDSL 603
           L V+DT + E EL ALKD++
Sbjct: 126 LFVMDTAVDESELTALKDAV 145


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 37/133 (27%), Positives = 51/133 (38%), Gaps = 11/133 (8%)
 Frame = +1

Query: 148 DMATYEEFIQQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKE-RPDLPPIQY-- 318
           D   YE   Q N     ++FT    P++        +PL  + QP  E RPD  P+    
Sbjct: 151 DFVGYE---QGNSSSKFVQFTSYAIPATNDVCNASGIPLGMIVQPFAELRPDEAPVPVVD 207

Query: 319 ----EPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPFPPQ--YAAISEQHQPAEL 480
                P  C +  CR  +NP  Q    +  W CN C   N F        +  QH   E 
Sbjct: 208 NTNSNPPRCKK--CRGYINPFIQFTMASSKWTCNLCGSENSFNDDGFNPLVGSQHDGVES 265

Query: 481 IP--NFSTIEYTI 513
            P  N  TI++ +
Sbjct: 266 RPELNVGTIDFKV 278


>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 926

 Score = 32.7 bits (71), Expect = 0.043
 Identities = 21/85 (24%), Positives = 34/85 (40%), Gaps = 3/85 (3%)
 Frame = +1

Query: 199 IRFTWNVWPSSRIEATRLVVPLACLYQP---LKERPDLPPIQYEPVLCTRNTCRAILNPM 369
           +R T N  P++     +  +P A + +P   L E  D  P+  + ++     CR  +NP 
Sbjct: 207 VRSTINCVPTTNSLLKKSKIPFALVIRPYTSLVEEDDPVPVVTDTIISRCRRCRMYINPF 266

Query: 370 CQVDYRAKLWVCNFCFQRNPFPPQY 444
                    + CN C   N  P  Y
Sbjct: 267 SIFIDNGHRYRCNSCGIVNEVPQSY 291


>SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 551

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 13/46 (28%), Positives = 22/46 (47%)
 Frame = +1

Query: 427 PFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIYLLVVDTC 564
           P+ P Y ++S Q  P+ +      + YTI R +A+P    +    C
Sbjct: 2   PYNPLYESLSNQFDPSRIETVLDPMGYTIKR-RALPVSLTIAGSDC 46


>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
           Alp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 784

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 4/68 (5%)
 Frame = +1

Query: 412 CFQRNPFPPQYAAISEQ-HQPAELIPNFS--TIE-YTITRAQAMPPIYLLVVDTCLXEEE 579
           C     F   Y  +  Q    + + PNFS  T+  YT+  ++++  +YL++ D  L  E+
Sbjct: 154 CAALRKFVMDYVVLIMQCENQSRIDPNFSLQTLRLYTLPTSRSLRQVYLILRDLLLSMEK 213

Query: 580 LGALKDSL 603
             +  D L
Sbjct: 214 NASSSDDL 221


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,618,870
Number of Sequences: 5004
Number of extensions: 55459
Number of successful extensions: 135
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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