BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_N16 (605 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 196 3e-51 SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Sc... 155 6e-39 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 48 1e-06 SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 33 0.043 SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyce... 26 3.7 SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 26 4.9 >SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein Sec23a|Schizosaccharomyces pombe|chr 3|||Manual Length = 759 Score = 196 bits (477), Expect = 3e-51 Identities = 83/143 (58%), Positives = 107/143 (74%) Frame = +1 Query: 175 QQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRA 354 ++ E+RDG+RFTWNV+PS+RIE++R +VP+A +Y+PL ERPDLPP+ YEPV C + C+A Sbjct: 4 EEIEERDGVRFTWNVFPSTRIESSRTIVPIASIYKPLNERPDLPPVLYEPVTC-KAPCKA 62 Query: 355 ILNPMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMP 534 +LNP C +D RAK W+C FC QRN PPQY IS P EL+P +STIEYT+ R + Sbjct: 63 VLNPYCHIDTRAKFWICPFCLQRNMLPPQYKDISNTSLPIELLPEYSTIEYTLPRPPQLT 122 Query: 535 PIYLLVVDTCLXEEELGALKDSL 603 P++L VVD C EE L ALKDSL Sbjct: 123 PVFLFVVDVCQDEENLQALKDSL 145 >SPBC776.04 |sec2302|sec23-b|GTPase activating protein Sec23b |Schizosaccharomyces pombe|chr 2|||Manual Length = 765 Score = 155 bits (375), Expect = 6e-39 Identities = 67/140 (47%), Positives = 93/140 (66%) Frame = +1 Query: 184 EDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKERPDLPPIQYEPVLCTRNTCRAILN 363 ED+DGIR +WN + ++ E R V+P+A +Y PL E + QY+PV C R CRA+LN Sbjct: 7 EDQDGIRLSWNTFSATPAENARAVIPIAAMYTPLHENERMTIEQYDPVAC-RAPCRAVLN 65 Query: 364 PMCQVDYRAKLWVCNFCFQRNPFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIY 543 P C VD RA+ W+C FCFQRNP P QY+ IS P EL+ +T+EY +++ PP++ Sbjct: 66 PYCHVDLRARFWICPFCFQRNPLPAQYSDISSNSLPLELLSQSTTMEYVLSKPVKSPPVF 125 Query: 544 LLVVDTCLXEEELGALKDSL 603 L V+DT + E EL ALKD++ Sbjct: 126 LFVMDTAVDESELTALKDAV 145 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 48.0 bits (109), Expect = 1e-06 Identities = 37/133 (27%), Positives = 51/133 (38%), Gaps = 11/133 (8%) Frame = +1 Query: 148 DMATYEEFIQQNEDRDGIRFTWNVWPSSRIEATRLVVPLACLYQPLKE-RPDLPPIQY-- 318 D YE Q N ++FT P++ +PL + QP E RPD P+ Sbjct: 151 DFVGYE---QGNSSSKFVQFTSYAIPATNDVCNASGIPLGMIVQPFAELRPDEAPVPVVD 207 Query: 319 ----EPVLCTRNTCRAILNPMCQVDYRAKLWVCNFCFQRNPFPPQ--YAAISEQHQPAEL 480 P C + CR +NP Q + W CN C N F + QH E Sbjct: 208 NTNSNPPRCKK--CRGYINPFIQFTMASSKWTCNLCGSENSFNDDGFNPLVGSQHDGVES 265 Query: 481 IP--NFSTIEYTI 513 P N TI++ + Sbjct: 266 RPELNVGTIDFKV 278 >SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 926 Score = 32.7 bits (71), Expect = 0.043 Identities = 21/85 (24%), Positives = 34/85 (40%), Gaps = 3/85 (3%) Frame = +1 Query: 199 IRFTWNVWPSSRIEATRLVVPLACLYQP---LKERPDLPPIQYEPVLCTRNTCRAILNPM 369 +R T N P++ + +P A + +P L E D P+ + ++ CR +NP Sbjct: 207 VRSTINCVPTTNSLLKKSKIPFALVIRPYTSLVEEDDPVPVVTDTIISRCRRCRMYINPF 266 Query: 370 CQVDYRAKLWVCNFCFQRNPFPPQY 444 + CN C N P Y Sbjct: 267 SIFIDNGHRYRCNSCGIVNEVPQSY 291 >SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 26.2 bits (55), Expect = 3.7 Identities = 13/46 (28%), Positives = 22/46 (47%) Frame = +1 Query: 427 PFPPQYAAISEQHQPAELIPNFSTIEYTITRAQAMPPIYLLVVDTC 564 P+ P Y ++S Q P+ + + YTI R +A+P + C Sbjct: 2 PYNPLYESLSNQFDPSRIETVLDPMGYTIKR-RALPVSLTIAGSDC 46 >SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 784 Score = 25.8 bits (54), Expect = 4.9 Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 4/68 (5%) Frame = +1 Query: 412 CFQRNPFPPQYAAISEQ-HQPAELIPNFS--TIE-YTITRAQAMPPIYLLVVDTCLXEEE 579 C F Y + Q + + PNFS T+ YT+ ++++ +YL++ D L E+ Sbjct: 154 CAALRKFVMDYVVLIMQCENQSRIDPNFSLQTLRLYTLPTSRSLRQVYLILRDLLLSMEK 213 Query: 580 LGALKDSL 603 + D L Sbjct: 214 NASSSDDL 221 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,618,870 Number of Sequences: 5004 Number of extensions: 55459 Number of successful extensions: 135 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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