BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_M16 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 205 4e-54 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 203 2e-53 SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 28 1.5 SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 2.6 SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 26 4.5 SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 26 6.0 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 26 6.0 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 205 bits (501), Expect = 4e-54 Identities = 92/184 (50%), Positives = 114/184 (61%) Frame = +2 Query: 140 VCHTDAYTLSGKSPEGVFPXXXXXXXXXXXXXXXXXXXXXKPGDHVVPLYVPQCNTCKFC 319 VCHTDAYTLSGK PEG+FP + GD V+ LY P+C TCKFC Sbjct: 49 VCHTDAYTLSGKDPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFC 108 Query: 320 LNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEA 499 + KTNLC ++R+TQG+G+MPDGT RF C G L HFMGCSTFS+YTVV +IS+ + Sbjct: 109 KSGKTNLCGRIRTTQGKGLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERL 168 Query: 500 APLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVD 679 APLD VCLLGCG+ TGYGAA TA ++ G + A+F RI G+D Sbjct: 169 APLDSVCLLGCGITTGYGAATITADIKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFGID 228 Query: 680 INPD 691 +NP+ Sbjct: 229 VNPE 232 Score = 45.2 bits (102), Expect = 9e-06 Identities = 19/43 (44%), Positives = 26/43 (60%) Frame = +1 Query: 10 TVGXVLQCLAAVAWAAGKPLSIXALXVXPPKAGAVRVKLTATG 138 T G ++ C AAVAW PLSI + V PP+ VR+K+ +G Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSG 48 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 203 bits (495), Expect = 2e-53 Identities = 92/184 (50%), Positives = 114/184 (61%) Frame = +2 Query: 140 VCHTDAYTLSGKSPEGVFPXXXXXXXXXXXXXXXXXXXXXKPGDHVVPLYVPQCNTCKFC 319 VCHTDAYTLSG PEG FP +PGDHV+ LY P+C CKFC Sbjct: 46 VCHTDAYTLSGVDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFC 105 Query: 320 LNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEA 499 + KTNLC K+R TQG+G+MPDGT RF C+ + L H+MGCS+FSQYTVV +ISL ++ + Sbjct: 106 RSGKTNLCSKIRETQGRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHS 165 Query: 500 APLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVD 679 APL +CLLGCGV TG+GA ++AKVE GS A+ +RII +D Sbjct: 166 APLRSICLLGCGVTTGFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAID 225 Query: 680 INPD 691 IN D Sbjct: 226 INAD 229 Score = 47.2 bits (107), Expect = 2e-06 Identities = 23/41 (56%), Positives = 27/41 (65%) Frame = +1 Query: 4 MSTVGXVLQCLAAVAWAAGKPLSIXALXVXPPKAGAVRVKL 126 MS G + C AAVAW A +PLSI + V PPKA VRVK+ Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKV 41 >SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 27.9 bits (59), Expect = 1.5 Identities = 31/144 (21%), Positives = 50/144 (34%), Gaps = 2/144 (1%) Frame = +2 Query: 266 GDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKG--QELYHFMGC 439 GD VV + C C FC + C ++ V G+ G + L GC Sbjct: 116 GDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDV-NYGSHHSAIFGYTKLLGDVPGC 174 Query: 440 STFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXX 619 EI+ CK+ + P D L V A +V+ G AI+ Sbjct: 175 QAEYIRVPFAEINCCKLPDDIP-DSEGLFMSDVLCTSLHACTLGEVKKGDTVAIWGMGPI 233 Query: 620 XXXXXXXXXXXXXNRIIGVDINPD 691 +++IG+++ P+ Sbjct: 234 GLYAGRWAQILGASKVIGIEVVPE 257 >SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr 2|||Manual Length = 1250 Score = 27.1 bits (57), Expect = 2.6 Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = +2 Query: 266 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 421 G HVV L V N C + KT KV + + P G+R++ C G L Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087 >SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1502 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/24 (45%), Positives = 13/24 (54%) Frame = +2 Query: 479 LCKVAEAAPLDKVCLLGCGVPTGY 550 +CKV E A L CLL C T + Sbjct: 979 ICKVREIASLSLTCLLDCSKMTTF 1002 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = +2 Query: 260 KPGDHVVPLYVPQCNTCKFCLNPKTNLC 343 KPGD V C C +C + + NLC Sbjct: 84 KPGDPVAVEPGCVCRLCDYCRSGRYNLC 111 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/47 (27%), Positives = 24/47 (51%) Frame = -2 Query: 596 RNLILVQLWQYSRRLHNL*VHRIPANKLYLMEQPLQLYREKFLEQLC 456 R+L V + R H L +H++ K + +Q ++ R+ L+ LC Sbjct: 210 RDLDAVACHERIARYHILCIHQLCEKKQFSAQQEVEQLRKGILQSLC 256 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,478,825 Number of Sequences: 5004 Number of extensions: 44495 Number of successful extensions: 96 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 93 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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