Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_L17
(792 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 68 6e-12
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 68 6e-12
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 67 1e-11
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 66 3e-11
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 61 7e-10
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 60 2e-09
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 58 5e-09
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 56 3e-08
At5g11330.1 68418.m01323 monooxygenase family protein low simila... 33 0.16
At2g13440.1 68415.m01483 glucose-inhibited division family A pro... 31 0.66
At3g51050.1 68416.m05590 FG-GAP repeat-containing protein 31 0.88
At5g62130.1 68418.m07798 Per1-like protein-related 29 4.7
At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl... 29 4.7
At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial a... 28 6.2
At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containi... 28 8.2
At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containi... 28 8.2
At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquino... 28 8.2
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 68.1 bits (159), Expect = 6e-12
Identities = 49/134 (36%), Positives = 77/134 (57%), Gaps = 3/134 (2%)
Frame = +2
Query: 329 ADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYSLITSTLLPNW 508
++ YD+IIVGGG+AGC LA L++ ++ VL++E G P + N + +TL
Sbjct: 50 SEDYYDYIIVGGGTAGCPLAATLSQ--SFRVLLLERGGVPYNRPNVMSHDGFLTTLT--- 104
Query: 509 GYFGVNDDFSSQGQKF---KSIRHTRGKMLGGSSSLNSMFYVRGNRADYDNWAENGNEGW 679
VN +F S Q F + + + RG++LGGSS++N+ FY +RAD + EN W
Sbjct: 105 ---DVN-NFDSPAQSFISEEGVPNARGRVLGGSSAINAGFY---SRAD-KQFFENSGLVW 156
Query: 680 DWNTVIQYFKKSER 721
D ++V Q ++ ER
Sbjct: 157 DLSSVNQSYEWVER 170
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 68.1 bits (159), Expect = 6e-12
Identities = 49/128 (38%), Positives = 74/128 (57%), Gaps = 4/128 (3%)
Frame = +2
Query: 338 SYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYSLITSTLLPNWGYF 517
S+D+I+VGGG+AGC LA L+E ++VL+IE G P G L+ +GY
Sbjct: 36 SFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSP------FGDPLVEDK--KYYGYS 85
Query: 518 GVN-DDFSSQGQKFKS---IRHTRGKMLGGSSSLNSMFYVRGNRADYDNWAENGNEGWDW 685
+N D++SS Q F S I++ RG++LGGSS++N FY R + D + + GWD
Sbjct: 86 LINTDEYSSVAQSFTSVDGIKNHRGRVLGGSSAINGGFYSRAS----DEFVKKA--GWDK 139
Query: 686 NTVIQYFK 709
+ V + +K
Sbjct: 140 DLVQESYK 147
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 66.9 bits (156), Expect = 1e-11
Identities = 51/141 (36%), Positives = 75/141 (53%), Gaps = 4/141 (2%)
Frame = +2
Query: 299 PLYPAHANVPADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYS 478
P A + S+D+I+VGGG+AGC LA L+E ++VL+IE G P G
Sbjct: 2 PYMTTDAKEVSGKSFDYIVVGGGTAGCSLAATLSE--KYSVLVIERGGSP------FGDP 53
Query: 479 LITSTLLPNWGYFGVN-DDFSSQGQKFKS---IRHTRGKMLGGSSSLNSMFYVRGNRADY 646
L+ +GY +N D++SS Q F S I + RG++LGGSS++N FY R +
Sbjct: 54 LVEER--KYFGYSLLNTDEYSSVAQSFTSVDGIENYRGRVLGGSSAINGGFYSRAS---- 107
Query: 647 DNWAENGNEGWDWNTVIQYFK 709
D + + GWD V + +K
Sbjct: 108 DEFVKKA--GWDKGLVQESYK 126
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 66.1 bits (154), Expect = 3e-11
Identities = 47/126 (37%), Positives = 67/126 (53%), Gaps = 6/126 (4%)
Frame = +2
Query: 326 PADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYSLITSTLLPN 505
P S YD+II+GGG+AGC LA L++ N +VL++E GD P N+P T L
Sbjct: 41 PTTSYYDYIIIGGGTAGCPLAATLSQ--NASVLLLERGDSP---YNNPNI-----TRLSA 90
Query: 506 WGYFGVNDDFSSQGQKFKS---IRHTRGKMLGGSSSLNSMFYVRGNRADYDNWAENG--- 667
+G + SS Q+F S + + R ++LGG S+LN+ FY R N +G
Sbjct: 91 FGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMGWDGALA 150
Query: 668 NEGWDW 685
NE + W
Sbjct: 151 NESYQW 156
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 61.3 bits (142), Expect = 7e-10
Identities = 42/107 (39%), Positives = 64/107 (59%), Gaps = 4/107 (3%)
Frame = +2
Query: 332 DSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYSLITSTLLPNWG 511
DSSYD+I++GGG+AGC LA L++ N++VL++E G P + AN + L N+
Sbjct: 60 DSSYDYIVIGGGTAGCPLAATLSQ--NFSVLVLERGGVPFTNAN--------VSFLRNF- 108
Query: 512 YFGVND-DFSSQGQKFKS---IRHTRGKMLGGSSSLNSMFYVRGNRA 640
+ G+ D SS Q F S + + R ++LGG S +N+ FY R + A
Sbjct: 109 HIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAA 155
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 60.1 bits (139), Expect = 2e-09
Identities = 40/116 (34%), Positives = 61/116 (52%), Gaps = 4/116 (3%)
Frame = +2
Query: 326 PADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPPSIANSPGYSLITSTLLPN 505
P ++S+D+II+GGG+AGC LA L++ N +VL++E G P + +TLL N
Sbjct: 68 PKNASFDYIIIGGGTAGCALAATLSQ--NASVLVLERGGSPYENPTATDMGNSVNTLLNN 125
Query: 506 ----WGYFGVNDDFSSQGQKFKSIRHTRGKMLGGSSSLNSMFYVRGNRADYDNWAE 661
W +++D + +TR ++LGG S +N FY R DY AE
Sbjct: 126 TPNSWSQLFISED---------GVYNTRPRVLGGGSVINGGFYSRAGN-DYVEEAE 171
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 58.4 bits (135), Expect = 5e-09
Identities = 41/133 (30%), Positives = 71/133 (53%), Gaps = 4/133 (3%)
Frame = +2
Query: 326 PADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDP---PSIANSPGYSLITSTL 496
P S +D+II+GGG+AGC LA L++ N TVL++E G P P+ + ++ +
Sbjct: 41 PKLSHFDYIIIGGGTAGCALAATLSQ--NATVLVLERGGSPYDDPAATDIGNFANTLLNI 98
Query: 497 LPN-WGYFGVNDDFSSQGQKFKSIRHTRGKMLGGSSSLNSMFYVRGNRADYDNWAENGNE 673
PN W +++D + ++R ++LGG + +N+ FY +RA+ D AE G E
Sbjct: 99 TPNSWSQLFISED---------GVFNSRARVLGGGTVINAGFY---SRAEEDFVAEAGWE 146
Query: 674 GWDWNTVIQYFKK 712
+ ++ +K
Sbjct: 147 RDEVEAAYEWVEK 159
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 56.0 bits (129), Expect = 3e-08
Identities = 34/105 (32%), Positives = 58/105 (55%), Gaps = 4/105 (3%)
Frame = +2
Query: 326 PADSSYDFIIVGGGSAGCVLANRLTEVANWTVLMIEAGDDP---PSIANSPGYSLITSTL 496
P + +D+II+GGG++GC LA L++ N +VL++E G P P+ + ++ S
Sbjct: 41 PMFARFDYIIIGGGTSGCALAATLSQ--NASVLVLERGGAPYDNPTATDIENFATTLSNT 98
Query: 497 LP-NWGYFGVNDDFSSQGQKFKSIRHTRGKMLGGSSSLNSMFYVR 628
P +W +++D + +TR ++LGG S LN+ FY R
Sbjct: 99 SPKSWSQLFISED---------GVYNTRARVLGGGSVLNAGFYTR 134
>At5g11330.1 68418.m01323 monooxygenase family protein low
similarity to 2,6-dihydroxypyridine 3-hydroxylase (DHPH)
[GI:14495302] [Arthrobacter nicotinovorans]; contains
Pfam profile PF01360: Monooxygenase
Length = 408
Score = 33.5 bits (73), Expect = 0.16
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +2
Query: 350 IIVGGGSAGCVLANRLTEVANWTVLMIEAGDDPP 451
IIVGG AG A+ LT +A W VL++E +PP
Sbjct: 9 IIVGGSIAGLSCAHSLT-LACWDVLVLEKSSEPP 41
>At2g13440.1 68415.m01483 glucose-inhibited division family A
protein similar to GidA from Pseudomonas syringae
[GI:10764670]; contains Pfam profile PF01134 Glucose
inhibited division protein A
Length = 723
Score = 31.5 bits (68), Expect = 0.66
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 329 ADSSYDFIIVGGGSAGCVLANRLTEVANWTVLM 427
+DS+YD I+VG G AGC A + T+L+
Sbjct: 69 SDSTYDVIVVGAGHAGCEAALASARLGASTLLL 101
>At3g51050.1 68416.m05590 FG-GAP repeat-containing protein
Length = 698
Score = 31.1 bits (67), Expect = 0.88
Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +2
Query: 425 MIEAGDDPPSIANSPGYSLITSTLLPNWGYFG-VNDDFSSQG 547
MI AG D ++ SPG S++ S LP+ + DDFS+ G
Sbjct: 598 MILAGGDQAAVIISPGGSILASIELPSQPTHALITDDFSNDG 639
>At5g62130.1 68418.m07798 Per1-like protein-related
Length = 343
Score = 28.7 bits (61), Expect = 4.7
Identities = 16/59 (27%), Positives = 29/59 (49%)
Frame = +2
Query: 131 KFDWTSATVLKSAKTFECVLIMFLQAIALFQVKILIPILEVIQLLIIALSSFEIGEPLY 307
+ D++SATVL +L F ++ + PIL V+ I+ L+ + + E L+
Sbjct: 193 RLDYSSATVLAGFSLILAILRSFSIQDQSVKIMVTAPILAVVATHILYLNFYNLDEGLH 251
>At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl
CoA:diacylglycerol acyltransferase (DGAT) identical to
gi:5050913, gi:6625553
Length = 520
Score = 28.7 bits (61), Expect = 4.7
Identities = 17/69 (24%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = +2
Query: 140 WTSATVLKSAKTFECVLIMFLQAIALFQVKILIPILEVIQLLIIALS-SFEIGEPLYPAH 316
W S+ L+ F C + + + +A F V+ L+ + + ++I L + E LYP +
Sbjct: 167 WFSSRSLRDWPLFMCCISLSIFPLAAFTVEKLVLQKYISEPVVIFLHIIITMTEVLYPVY 226
Query: 317 ANVPADSSY 343
+ DS++
Sbjct: 227 VTLRCDSAF 235
>At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial almost
identical to Lon protease homolog 2 mitochondrial
precursor SP:P93655, GI:1848290 from [Arabidopsis
thaliana]
Length = 940
Score = 28.3 bits (60), Expect = 6.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +2
Query: 599 SSLNSMFYVRGNRADYDNWAENGNEGWDWNTVIQYFK 709
S + ++ + NR D+D AEN EG + + V Y K
Sbjct: 891 SQIKTIIFPEANRRDFDELAENVKEGLNVHFVDDYGK 927
>At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 852
Score = 27.9 bits (59), Expect = 8.2
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +2
Query: 299 PLYPAHANVPADSSYDFI-IVGGGSAGCVL 385
P YP+H +VP S DFI + G SAG V+
Sbjct: 356 PGYPSHGSVPVVVSPDFIPQLSGPSAGSVV 385
>At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile:PF00806 Pumilio-family RNA
binding domains
Length = 861
Score = 27.9 bits (59), Expect = 8.2
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +2
Query: 299 PLYPAHANVPADSSYDFI-IVGGGSAGCVL 385
P YP+H +VP S DFI + G SAG V+
Sbjct: 356 PGYPSHGSVPVVVSPDFIPQLSGPSAGSVV 385
>At2g43400.1 68415.m05394 electron transfer flavoprotein-ubiquinone
oxidoreductase family protein contains Pfam profile:
PF05187 Electron transfer flavoprotein-ubiquinone
oxidoreductase
Length = 633
Score = 27.9 bits (59), Expect = 8.2
Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 7/63 (11%)
Frame = +2
Query: 341 YDFIIVGGGSAGCVLANRLTEVA-----NWTVLMIEAGDDPPS--IANSPGYSLITSTLL 499
YD +IVG G AG A RL +++ + +V ++E G + I+ + L LL
Sbjct: 100 YDVLIVGAGPAGLSAAIRLKQLSQEKNIDLSVCVVEKGAEVGGHIISGNVFEPLALDELL 159
Query: 500 PNW 508
P+W
Sbjct: 160 PHW 162
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,643,453
Number of Sequences: 28952
Number of extensions: 314072
Number of successful extensions: 877
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 872
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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