BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_L16 (618 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 127 1e-30 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 2.9 SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 26 5.0 SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 6.6 SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schiz... 25 8.8 SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr... 25 8.8 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 8.8 SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 25 8.8 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 127 bits (307), Expect = 1e-30 Identities = 72/171 (42%), Positives = 90/171 (52%), Gaps = 1/171 (0%) Frame = +1 Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAP-AANLPHVLRWYNQIASYTSAERKTW 285 L LN +L +KS++ GY PSQAD VF+ VG AP A P+ RWY QIA+Y A Sbjct: 12 LKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQIATYDLA----- 66 Query: 286 SQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLKAYADKKSKK 465 T P +DLFGS + Y KK+ K Sbjct: 67 ---TLPGTAKEVSAYGPEGAAAAEEDEIDLFGSDEEEDPEAERIKAERVAE-YNKKKAAK 122 Query: 466 PALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWGASKLVPVGYGI 618 P + KS + LDVKPWDDET M E+E VR I+M+GL+WG SKLVPVG+G+ Sbjct: 123 PKAVHKSLVTLDVKPWDDETPMDELEKAVRSIQMDGLVWGLSKLVPVGFGV 173 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 26.6 bits (56), Expect = 2.9 Identities = 14/37 (37%), Positives = 18/37 (48%), Gaps = 1/37 (2%) Frame = -1 Query: 594 FGGSPEKAFHFNXAYLVFHFLHIGFIIP-WLDIKENR 487 FG S HF+ Y VF IG I P W++ N+ Sbjct: 485 FGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNK 521 >SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 475 Score = 25.8 bits (54), Expect = 5.0 Identities = 21/66 (31%), Positives = 28/66 (42%), Gaps = 4/66 (6%) Frame = -3 Query: 247 DYTIVVRGEG*RPAPCQLAQILEHQLEK----ECIQTRSFSQLNIGSSH*XPWAVLTSPT 80 +Y +VV G G P C + L QLE E + F + ++ P A L S Sbjct: 308 NYPVVVNGNGVNPYLCDVQAFLTSQLEYYFSIENLCKDMFLRKHMDDEGYVPLAFLASFN 367 Query: 79 AIVSFS 62 I SFS Sbjct: 368 RIKSFS 373 >SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.4 bits (53), Expect = 6.6 Identities = 11/19 (57%), Positives = 12/19 (63%) Frame = +2 Query: 308 PPVLNPRLPPQQRKTTMTT 364 PPVL P LPP Q T T+ Sbjct: 449 PPVLLPTLPPIQTTTIQTS 467 >SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 25.0 bits (52), Expect = 8.8 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = +1 Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVF 189 L+ +++YL ++++ Y PS+ D Q F Sbjct: 300 LSLVSKYLLVSAFLASYNPSRLDAQFF 326 >SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr 2|||Manual Length = 667 Score = 25.0 bits (52), Expect = 8.8 Identities = 12/37 (32%), Positives = 19/37 (51%) Frame = -1 Query: 297 GALRPCFAFSRSV*SNLIIPS*YVGKVSGRRLANLLK 187 G L F ++ + +I+PS +G GR + LLK Sbjct: 394 GLLLTSATFGAAIPTGIIVPSLAIGACIGRAVGTLLK 430 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.0 bits (52), Expect = 8.8 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 8/82 (9%) Frame = +2 Query: 239 GIIKLLHTLRLNAKHGLRAPAH*PPVLNPRL--------PPQQRKTTMTTTLIYLVLVTR 394 GI+ LR+N KH LR H V+N L K +L+ V + Sbjct: 15 GILLSFEQLRINFKHILRHLEHESHVINSTLTTLISQENASMDEKIEKIDSLLSRVSTVK 74 Query: 395 KRMQKLNEFEKNV*KHMLTRSL 460 K+M+ L++ E K +R L Sbjct: 75 KKMKHLHDCEALFIKQTKSRLL 96 >SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 25.0 bits (52), Expect = 8.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +1 Query: 448 DKKSKKPALIAKSSILLDVKPWDDETDMKEME 543 DK+S P LIA++ + ++ D+ +KE E Sbjct: 692 DKESNSPVLIAETRLFNNLFKCDNPAALKEQE 723 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,325,019 Number of Sequences: 5004 Number of extensions: 41729 Number of successful extensions: 102 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 99 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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