Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_L16
(618 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 111 3e-25
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 109 2e-24
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 106 1e-23
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 104 4e-23
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.050
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.61
At5g38990.1 68418.m04717 protein kinase family protein contains ... 30 1.4
At5g39000.1 68418.m04718 protein kinase family protein contains ... 29 3.3
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 28 4.3
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 4.3
At5g45280.2 68418.m05559 pectinacetylesterase, putative similar ... 27 7.5
At5g45280.1 68418.m05558 pectinacetylesterase, putative similar ... 27 7.5
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 7.5
At2g36270.1 68415.m04452 bZIP transcription factor family protei... 27 10.0
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 27 10.0
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 111 bits (268), Expect = 3e-25
Identities = 70/181 (38%), Positives = 93/181 (51%), Gaps = 11/181 (6%)
Frame = +1
Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-----ASYTSAE 273
L L+++L +SY++GY S+ D+ VF + K P + +V RW+N I S SAE
Sbjct: 13 LKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDALLRISGVSAE 72
Query: 274 RK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXXXXXXXXXXXL 435
+G+SP+ VDLFG
Sbjct: 73 GSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKKAAEE------ 126
Query: 436 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWGASKLVPVGYG 615
+A + K S K KSS+L+D+KPWDDETDMK++E VR I+MEGL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYG 186
Query: 616 I 618
I
Sbjct: 187 I 187
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 109 bits (261), Expect = 2e-24
Identities = 68/181 (37%), Positives = 92/181 (50%), Gaps = 11/181 (6%)
Frame = +1
Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-----ASYTSAE 273
L L+++L +SY++GY S+ D+ VF + K P + + RWYN I S SAE
Sbjct: 13 LKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDALLRISGVSAE 72
Query: 274 RK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXXXXXXXXXXXL 435
+G++P+ VDLFG
Sbjct: 73 GSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKKAAEE------ 126
Query: 436 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWGASKLVPVGYG 615
+A + K S K KSS+L+D+KPWDDETDMK++E V+ I+MEGL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGASKLVPVGYG 186
Query: 616 I 618
I
Sbjct: 187 I 187
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 106 bits (255), Expect = 1e-23
Identities = 63/173 (36%), Positives = 85/173 (49%), Gaps = 3/173 (1%)
Frame = +1
Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTSAE---RK 279
+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS + +
Sbjct: 14 VKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLAKSFPGKA 73
Query: 280 TWSQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLKAYADKKS 459
Q +DLFG + A K +
Sbjct: 74 VGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------REAAKKDT 127
Query: 460 KKPALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWGASKLVPVGYGI 618
KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WGASKLVPVGYGI
Sbjct: 128 KKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGI 180
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 104 bits (250), Expect = 4e-23
Identities = 64/179 (35%), Positives = 87/179 (48%), Gaps = 7/179 (3%)
Frame = +1
Query: 103 RXLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTSAERKT 282
R L L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+ +
Sbjct: 12 RGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLAKSFPG 71
Query: 283 WSQGTS-------PLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLKA 441
+ G P +DLF +
Sbjct: 72 KADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE------RE 125
Query: 442 YADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWGASKLVPVGYGI 618
A K +KK KSS+LL+VKPWDDETDMK++E VR ++M GL WGASKLVPVGYGI
Sbjct: 126 AAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYGI 184
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.050
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = +1
Query: 109 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 252
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.61
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -1
Query: 585 SPEKAFHFNXAYLVFHFLHIGFIIPW 508
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 29.9 bits (64), Expect = 1.4
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +2
Query: 221 FPTYYDGIIKLLHTLRLNAKHGLRAPAH*PPVLNPRLPPQ 340
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 28.7 bits (61), Expect = 3.3
Identities = 14/56 (25%), Positives = 28/56 (50%)
Frame = +2
Query: 224 PTYYDGIIKLLHTLRLNAKHGLRAPAH*PPVLNPRLPPQQRKTTMTTTLIYLVLVT 391
P YYD I+ + L++N G A + P+++P L P + + +++ +T
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNRATPRIRKNKSHILPIT 445
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.3 bits (60), Expect = 4.3
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1
Query: 454 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLWG 585
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 4.3
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +1
Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 204
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At5g45280.2 68418.m05559 pectinacetylesterase, putative similar to
pectinacetylesterase precursor GI:1431629 from [Vigna
radiata]
Length = 391
Score = 27.5 bits (58), Expect = 7.5
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = -3
Query: 325 WV*HRRSMGWCPETMFCVQPK 263
W+ H GWC + CVQ K
Sbjct: 60 WIVHMEGGGWCTDIATCVQRK 80
>At5g45280.1 68418.m05558 pectinacetylesterase, putative similar to
pectinacetylesterase precursor GI:1431629 from [Vigna
radiata]
Length = 370
Score = 27.5 bits (58), Expect = 7.5
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = -3
Query: 325 WV*HRRSMGWCPETMFCVQPK 263
W+ H GWC + CVQ K
Sbjct: 60 WIVHMEGGGWCTDIATCVQRK 80
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 7.5
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +1
Query: 109 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 222
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g36270.1 68415.m04452 bZIP transcription factor family protein /
ABA-responsive element-binding protein, putative similar
to ABA-responsive element binding protein 1 (AREB1)
GI:9967417 from [Arabidopsis thaliana]; contains a bZIP
transcription factor basic domain signature (PDOC00036)
Length = 442
Score = 27.1 bits (57), Expect = 10.0
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 342 CWGGSRGFSTGGQWAGALRP 283
C+GG GF GGQ G + P
Sbjct: 293 CYGGGVGFGAGGQQMGMVGP 312
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 27.1 bits (57), Expect = 10.0
Identities = 9/36 (25%), Positives = 18/36 (50%)
Frame = +1
Query: 475 IAKSSILLDVKPWDDETDMKEMENQVRXIEMEGLLW 582
+ S++L D+K WD+ M+ + ++ G W
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSW 570
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,468,857
Number of Sequences: 28952
Number of extensions: 228802
Number of successful extensions: 604
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 595
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 603
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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