BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_K09 (729 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 84 2e-17 SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 84 2e-17 SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.7 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 4.8 SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 26 6.3 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 26 6.3 SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.4 >SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 109 Score = 84.2 bits (199), Expect = 2e-17 Identities = 36/50 (72%), Positives = 43/50 (86%) Frame = +1 Query: 559 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 708 RKSE+EYYA+L+K VHHY+G NI+LGTACGK +RV LAITD GDSDI+ Sbjct: 58 RKSELEYYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107 Score = 79.8 bits (188), Expect = 4e-16 Identities = 37/55 (67%), Positives = 44/55 (80%) Frame = +2 Query: 71 VAAKKQXKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 235 V KK K+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLR Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLR 58 >SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 84.2 bits (199), Expect = 2e-17 Identities = 40/59 (67%), Positives = 47/59 (79%) Frame = +2 Query: 59 APKMVAAKKQXKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 235 AP +KK K+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLR Sbjct: 8 APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66 Score = 83.4 bits (197), Expect = 3e-17 Identities = 35/50 (70%), Positives = 43/50 (86%) Frame = +1 Query: 559 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 708 RKSE+EYYA+L++ VHHYSGNNI+LGTACGK +RV LA+ D GDSDI+ Sbjct: 66 RKSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115 >SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 27.1 bits (57), Expect = 2.7 Identities = 9/26 (34%), Positives = 16/26 (61%) Frame = +2 Query: 131 MKSGKYCLGYKQTLKTLRQGKAKLVI 208 + SGKYC G T+ L++G + ++ Sbjct: 293 LDSGKYCFGVVDTMNALQEGAVETLL 318 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 26.2 bits (55), Expect = 4.8 Identities = 17/58 (29%), Positives = 32/58 (55%) Frame = +2 Query: 59 APKMVAAKKQXKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 232 APK + KK KT++ + + ++ G K+ +K +R+G+ LVI+A + P+ Sbjct: 33 APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82 >SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Spt6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1365 Score = 25.8 bits (54), Expect = 6.3 Identities = 23/75 (30%), Positives = 33/75 (44%) Frame = +1 Query: 400 FWAYVSFKQIIFNLPLMINYKFD*IAFVS*YCFAGSQCGEPME*SLNSILHFGRKSEIEY 579 FW+ S KQ I L + D V +C A S + ++ LN +HF +I Sbjct: 373 FWSLHSKKQDILKLYSDLGINDD---LVVPFCEAASSL-DAID-DLNDYIHFTYSEQIRD 427 Query: 580 YALLAKTGVHHYSGN 624 ALL TG+ G+ Sbjct: 428 RALLMGTGLRRPQGS 442 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 25.8 bits (54), Expect = 6.3 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 2/59 (3%) Frame = +3 Query: 177 LFDKAKQSWLSSLKMLLR*GEATEK*SFPILV--KGMHMESY*QRSMDSLCGLVKQFAK 347 +FD +++S L+S+K R K + PIL+ K H ++ + + + +++AK Sbjct: 89 MFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYDHFMTFPREDQEEITKQARRYAK 147 >SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 101 Score = 25.4 bits (53), Expect = 8.4 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +3 Query: 471 NCIRFLILFCWITMWGTYGVVFKLDTSLWQ 560 N + F+I+F +T W ++ KL SLW+ Sbjct: 50 NTVVFIIVF--LTGWAAKSIIVKLLPSLWR 77 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,967,671 Number of Sequences: 5004 Number of extensions: 58311 Number of successful extensions: 113 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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