BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_J24 (513 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 2e-63 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 4e-33 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 1e-31 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 8e-30 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.2 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.2 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 2.9 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 3.8 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 5.0 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.0 SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 25 6.7 SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schi... 25 6.7 SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc... 25 8.8 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 8.8 SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 235 bits (576), Expect = 2e-63 Identities = 101/132 (76%), Positives = 117/132 (88%) Frame = +3 Query: 45 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 224 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 225 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 404 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 405 RKEAESCDCLQG 440 R+EAE+CD LQG Sbjct: 121 RREAEACDALQG 132 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 135 bits (326), Expect = 4e-33 Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%) Frame = +3 Query: 45 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 218 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 219 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 398 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 399 VVRKEAESCDCLQG 440 +R+ A++C LQG Sbjct: 121 KIRRIADNCSGLQG 134 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 130 bits (314), Expect = 1e-31 Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%) Frame = +3 Query: 45 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 206 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 207 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 386 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 387 SVLDVVRKEAESCDCLQG 440 SVL+ +R+ A++C LQG Sbjct: 121 SVLERIRRMADNCSGLQG 138 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 124 bits (299), Expect = 8e-30 Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%) Frame = +3 Query: 48 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 227 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 228 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 401 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 402 VRKEAESCDCLQG 440 + +EA+ D L+G Sbjct: 122 IDREADGSDSLEG 134 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 1.2 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +3 Query: 348 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 446 A+GH G ELV + D +RK++E+ L+ P Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 1.6 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +3 Query: 84 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 185 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.2 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 279 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 380 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.2 bits (55), Expect = 2.9 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -3 Query: 421 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 284 ++A+ RTTS T+ +PS L P P + S R+ CPK Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 3.8 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +3 Query: 300 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 431 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 25.4 bits (53), Expect = 5.0 Identities = 15/46 (32%), Positives = 27/46 (58%) Frame = -2 Query: 197 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 60 ++Y+DA L +++ + G A V DLP L +D V++L S+ + Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 5.0 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -3 Query: 337 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 242 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 25.0 bits (52), Expect = 6.7 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = -2 Query: 98 SSDLVAALTSLDMYDFPHFVLFM*TINDLTLK 3 S D+ ++D DFP++V ND+T+K Sbjct: 194 SKDIKNETPTIDRCDFPNYVPMFAFPNDITIK 225 >SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 25.0 bits (52), Expect = 6.7 Identities = 17/39 (43%), Positives = 22/39 (56%) Frame = -2 Query: 386 IDELSTLRVVSLGPVVTGAGLSEDEVVRTEDLSERSRAD 270 +D +LR VSL T AGLS DEV + S+ S A+ Sbjct: 369 LDFCDSLRRVSLHVDPTLAGLSPDEVENFYNKSKNSLAN 407 >SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosaccharomyces pombe|chr 3|||Manual Length = 1014 Score = 24.6 bits (51), Expect = 8.8 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -2 Query: 329 GLSEDEVVRTEDLSERSRADRVHGAGLQVDEDG 231 G+S E+ R +D S+ R +RVH DEDG Sbjct: 240 GISGFEINRQKDTSDMKRNNRVH----MDDEDG 268 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 24.6 bits (51), Expect = 8.8 Identities = 14/61 (22%), Positives = 26/61 (42%) Frame = +3 Query: 288 GQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGIPTDTLARR 467 G+ F P N N G Y GA +D ++ + ++A+ + P D +A+ Sbjct: 323 GEPFNPANMFSNIFNLSGN--PGDYAWGARGLDDIISQLMEQAQGHNAPAPAPEDVIAKM 380 Query: 468 R 470 + Sbjct: 381 K 381 >SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual Length = 1279 Score = 24.6 bits (51), Expect = 8.8 Identities = 14/46 (30%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = +3 Query: 333 AGNNWA-KGHYTEGAELVDSVLDVVRKEAESCDCLQGIPTDTLARR 467 + + WA +GH + + S+ +++ E+C GIPT+ L RR Sbjct: 1177 SSSEWAVRGHKENVEKAIASLEKSIKQVMENCIAYLGIPTN-LHRR 1221 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,844,746 Number of Sequences: 5004 Number of extensions: 34771 Number of successful extensions: 115 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 112 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 206265012 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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