SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_J11
         (589 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...   188   5e-49
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...   100   2e-22
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    93   2e-20
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    92   6e-20
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    92   6e-20
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    83   4e-17
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    76   3e-15
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    75   8e-15
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    73   2e-14
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    66   3e-12
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    64   2e-11
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    60   2e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    33   0.023
SPAC328.08c |||tubulin specific chaperone cofactor C |Schizosacc...    27   1.5  
SPBC19F8.06c |meu22||amino acid permease, unknown 11|Schizosacch...    27   2.0  
SPAC4F10.04 |||protein phosphatase type 2A, intrinsic regulator ...    25   8.2  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    25   8.2  

>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score =  188 bits (458), Expect = 5e-49
 Identities = 85/155 (54%), Positives = 108/155 (69%)
 Frame = +3

Query: 45  SAAQLXITKALNXLNLPKTCNTEFPDPDDLLNFKLIICPDEGFYRGGKFVFSFKVGPNYP 224
           S AQ+ I K +  L +P T +T +PDP  L    L I PDEG+Y+GGKF F  ++  NYP
Sbjct: 22  SPAQIRIQKDVTDLEIPSTMSTSWPDPIKLNVLHLEIRPDEGYYKGGKFKFRIQIDDNYP 81

Query: 225 HEPPKVKCETAVYHPNIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNK 404
           H+PPKVKC   +YHPNID+EGNVCLNILR+DW PVL +NSI+ GLQ+LFL PN EDPLNK
Sbjct: 82  HDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILVGLQFLFLSPNAEDPLNK 141

Query: 405 EAADELQSNRRLFEQHVQRAMRGGYVGTSYFERCL 509
           EAA +L  + + F   V+ AM+GG V    F+  +
Sbjct: 142 EAAADLHKDPQGFASRVRTAMKGGLVNGISFDNVM 176


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score =  100 bits (239), Expect = 2e-22
 Identities = 46/119 (38%), Positives = 71/119 (59%), Gaps = 1/119 (0%)
 Frame = +3

Query: 93  PKTCNTEFPDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHP 269
           P +C+   P  DDL +++  I  P +  Y GG F  S     +YP +PPKV   T +YHP
Sbjct: 18  PSSCSAG-PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHP 76

Query: 270 NIDLEGNVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFE 446
           NI+  G++CL+ILR+ W P LT++ ++  +  L  +PNP+DPL  E A   +++R  +E
Sbjct: 77  NINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYE 135


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 93.5 bits (222), Expect = 2e-20
 Identities = 41/98 (41%), Positives = 63/98 (64%), Gaps = 1/98 (1%)
 Frame = +3

Query: 159 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILRED-WKPVLT 335
           P +  Y GGKF FS K   +YP +PP ++  T +YHPN D EGNVCL IL++  +KP + 
Sbjct: 46  PSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIK 105

Query: 336 VNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQ 449
           + S++  +  L  EPNP+DPL    A++ +++R  F++
Sbjct: 106 LRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDK 143


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 91.9 bits (218), Expect = 6e-20
 Identities = 40/106 (37%), Positives = 61/106 (57%), Gaps = 1/106 (0%)
 Frame = +3

Query: 117 PDPDDLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNV 293
           P  D+L  FK+ +  P +  Y GGKF     +   YP  PP V+  T +YHPN+D  G +
Sbjct: 26  PTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRI 85

Query: 294 CLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSN 431
           CL+ L++DW P L + +++  +Q L   PNP+DPL+ + A   + N
Sbjct: 86  CLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVAKIWKEN 131


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 91.9 bits (218), Expect = 6e-20
 Identities = 43/103 (41%), Positives = 63/103 (61%), Gaps = 2/103 (1%)
 Frame = +3

Query: 114 FPDPD-DLLNFK-LIICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEG 287
           FPD D +LL++   I  P + +Y G KF  S     NYP+ PP +   + ++HPN+D+ G
Sbjct: 51  FPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSG 110

Query: 288 NVCLNILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAAD 416
           N+CL+IL++ W  V  V +I+  LQ L  EPN   PLN +AA+
Sbjct: 111 NICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAE 153


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 82.6 bits (195), Expect = 4e-17
 Identities = 39/108 (36%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
 Frame = +3

Query: 126 DDLLNFKLIICPDEGF-YRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLE-GNVCL 299
           DD+ + K +    EG  Y GG FV   ++  +YP  PPK+  +T +YHPN+  + G +CL
Sbjct: 31  DDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICL 90

Query: 300 NILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLF 443
           +IL++ W PV T+ S +  LQ L   P P +P + + A     N + F
Sbjct: 91  DILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQF 138


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 76.2 bits (179), Expect = 3e-15
 Identities = 47/159 (29%), Positives = 80/159 (50%), Gaps = 16/159 (10%)
 Frame = +3

Query: 42  ASAAQLXITKALNXL--NLPKTCNTEFPDPDDLLNFK-LIICPDEGFYRGGKFVFSFKVG 212
           +SA++  + K L  +  N P+  +    D   +  ++ +II P++  Y GG F  +    
Sbjct: 3   SSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFP 62

Query: 213 PNYPHEPPKVKCETAVYHPNIDLEGNVCLNILR-------------EDWKPVLTVNSIVY 353
            +YP  PPK+K  T ++HPN+   G VC++IL              E W PV +  +I+ 
Sbjct: 63  QDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILI 122

Query: 354 GLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMR 470
            +  +   PN E P N +AA E + N + F++ V+R +R
Sbjct: 123 SVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVR 161


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 74.9 bits (176), Expect = 8e-15
 Identities = 34/111 (30%), Positives = 59/111 (53%)
 Frame = +3

Query: 147 LIICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNILREDWKP 326
           +II P +  +  G F         YP++PP VK  + ++HPN+   G +CL+IL+  W P
Sbjct: 39  VIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSP 98

Query: 327 VLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMRGGY 479
              V +I+  +Q L  +PN   P N EAA   + N++ + + V++ +   +
Sbjct: 99  TYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSW 149


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 73.3 bits (172), Expect = 2e-14
 Identities = 38/117 (32%), Positives = 62/117 (52%), Gaps = 3/117 (2%)
 Frame = +3

Query: 129 DLLNFKL-IICPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLNI 305
           DL+N+K+ I    +  + GG +  +      YP  PPK +    ++HPN+   G VCL+I
Sbjct: 37  DLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSI 96

Query: 306 LRED--WKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLFEQHVQRAMR 470
           L E+  WKP +T+  I+ G+Q L  +PN   P   EA    + ++  +E+ V+   R
Sbjct: 97  LNEEEGWKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVRAQAR 153


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 35/102 (34%), Positives = 53/102 (51%), Gaps = 2/102 (1%)
 Frame = +3

Query: 129 DLLNFKLII-CPDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLN 302
           D+ ++K +I  P E  Y GG++V    V   YP  PP V  +T + HPNI    G VC++
Sbjct: 33  DMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMD 92

Query: 303 ILREDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQS 428
           IL+  W P  ++ S    +  L    +   PLN +AA  L++
Sbjct: 93  ILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRT 134


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 32/87 (36%), Positives = 46/87 (52%), Gaps = 2/87 (2%)
 Frame = +3

Query: 159 PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNID-LEGNVCLNILREDWKPVL- 332
           P E  Y GG +    ++   YP + P +     ++HPNID L G+VCL+++ + W P+  
Sbjct: 39  PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFD 98

Query: 333 TVNSIVYGLQYLFLEPNPEDPLNKEAA 413
            +N     L  L   PN  DPLN EAA
Sbjct: 99  MINIFEVFLPQLLRYPNASDPLNGEAA 125


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 38/129 (29%), Positives = 60/129 (46%), Gaps = 14/129 (10%)
 Frame = +3

Query: 126 DDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNVCLN 302
           DD   +  +I  PD   + GG +  + K   +YP  PP +K E   +HPN+  +G VC++
Sbjct: 33  DDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCIS 92

Query: 303 ILR-------------EDWKPVLTVNSIVYGLQYLFLEPNPEDPLNKEAADELQSNRRLF 443
           IL              E W PV +V  I+  +  +  EPN E   N +A    + +R  +
Sbjct: 93  ILHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLAEPNDESGANIDACKMWREDREEY 152

Query: 444 EQHVQRAMR 470
            + V+R  R
Sbjct: 153 CRVVRRLAR 161


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 33.5 bits (73), Expect = 0.023
 Identities = 22/85 (25%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
 Frame = +3

Query: 117 PDPDDLLNFKLIIC-PDEGFYRGGKFVFSFKVGPNYPHEPPKVKCETAVYHPNIDLEGNV 293
           P  +++L +  II  P +  Y GG++  +    P+YP +PP ++  T            +
Sbjct: 29  PATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRL 86

Query: 294 CLNIL---REDWKPVLTVNSIVYGL 359
           CL+      + W P   V++I+ GL
Sbjct: 87  CLSFSDFHPKSWNPSWMVSTILVGL 111


>SPAC328.08c |||tubulin specific chaperone cofactor C
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 259

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 13/59 (22%), Positives = 26/59 (44%)
 Frame = +1

Query: 364 IYSWNQTLKIPSTRKQRTSSKATGDFSNNTFRELCEAATSAHLISNDASNENLHTCKKC 540
           +Y  N TL + + + Q          +N   +   ++  S ++  ++ S+ NLH   KC
Sbjct: 114 VYFENDTLYLANLKNQNIGDNVIPYPNNKAVKVSAKSLRSCNISISNCSSVNLHNATKC 172


>SPBC19F8.06c |meu22||amino acid permease, unknown
           11|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 574

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 393 PLNKEAADELQSNRRLFEQHVQRAMRGGYVGTSYF 497
           PL  E   +LQ  R+L  +H+Q    GG VGT  F
Sbjct: 49  PLQPEDTQDLQ--RKLKPRHMQMIAIGGCVGTGLF 81


>SPAC4F10.04 |||protein phosphatase type 2A, intrinsic regulator
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 325

 Score = 25.0 bits (52), Expect = 8.2
 Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 2/39 (5%)
 Frame = +3

Query: 123 PDDLLNFKLII--CPDEGFYRGGKFVFSFKVGPNYPHEP 233
           PD +LN K++        ++   KF+   K GP Y H P
Sbjct: 237 PDAILNKKIVDEKANSNLYFSAIKFINVMKKGPFYEHSP 275


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 25.0 bits (52), Expect = 8.2
 Identities = 18/54 (33%), Positives = 24/54 (44%)
 Frame = +3

Query: 27  RLTKKASAAQLXITKALNXLNLPKTCNTEFPDPDDLLNFKLIICPDEGFYRGGK 188
           R   KA AA L  T     +      NT+FP   +LL  +LI+   + F R  K
Sbjct: 160 RSIMKAQAASLPFTPIYAAMTA--VINTKFPQIGELLLTRLIVQFRKSFQRNDK 211


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,288,989
Number of Sequences: 5004
Number of extensions: 47260
Number of successful extensions: 175
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -