Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_H22
(593 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 124 3e-29
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 37 0.009
At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 33 0.14
At1g75280.1 68414.m08745 isoflavone reductase, putative identica... 33 0.14
At2g44040.1 68415.m05475 dihydrodipicolinate reductase family pr... 32 0.25
At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 32 0.25
At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase fam... 31 0.77
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 29 1.8
At3g59890.2 68416.m06683 dihydrodipicolinate reductase family pr... 29 1.8
At3g59890.1 68416.m06684 dihydrodipicolinate reductase family pr... 29 1.8
At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SD... 29 2.3
At2g34070.1 68415.m04171 expressed protein 29 3.1
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 4.1
At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger) fa... 28 4.1
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 5.4
At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase fam... 28 5.4
At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong... 27 9.4
At5g50310.1 68418.m06229 kelch repeat-containing protein similar... 27 9.4
At5g44400.1 68418.m05436 FAD-binding domain-containing protein s... 27 9.4
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 27 9.4
At4g19170.1 68417.m02829 9-cis-epoxycarotenoid dioxygenase, puta... 27 9.4
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 124 bits (300), Expect = 3e-29
Identities = 62/141 (43%), Positives = 97/141 (68%)
Frame = +2
Query: 167 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLG 346
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG TGF+GRY+ +L
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGATGFLGRYLVQQLA 89
Query: 347 KIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRYSNVVINLVGRDY 526
K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI + +NVVINL+GR+Y
Sbjct: 90 KMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKAVMAKANVVINLIGREY 149
Query: 527 ETKNFKYNDVHVDGVRRIAXI 589
ET+NF + D + ++A +
Sbjct: 150 ETRNFSFEDANHHIAEKLALV 170
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 37.1 bits (82), Expect = 0.009
Identities = 28/97 (28%), Positives = 49/97 (50%), Gaps = 4/97 (4%)
Frame = +2
Query: 296 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 463
V G TG +G+ + + K G R D AQ ++ DLG + L D+
Sbjct: 7 VIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDK 65
Query: 464 ESIAKAVRYSNVVINLVGRDYETKNFKYNDVHVDGVR 574
ES+ KA++ +VVI+ VGR ++T+ ++ +D ++
Sbjct: 66 ESLVKAIKQVDVVISAVGR-FQTEILNQTNI-IDAIK 100
>At1g75290.1 68414.m08746 isoflavone reductase, putative similar to
SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-)
{Arabidopsis thaliana}; contains Pfam profile PF02716:
Isoflavone reductase
Length = 323
Score = 33.1 bits (72), Expect = 0.14
Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 296 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 466
V G TG +G+ + K G + L L D + K + T H L D E
Sbjct: 10 VIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLNDHE 69
Query: 467 SIAKAVRYSNVVINLVG 517
S+ KA++ ++VVI+ VG
Sbjct: 70 SLVKAIKQADVVISTVG 86
>At1g75280.1 68414.m08745 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: isoflavone reductase
Length = 310
Score = 33.1 bits (72), Expect = 0.14
Identities = 23/77 (29%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 296 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 466
V G TG++G+++ K G + L D + K + T H L D E
Sbjct: 10 VIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHE 69
Query: 467 SIAKAVRYSNVVINLVG 517
S+ KA++ +VVI+ VG
Sbjct: 70 SLVKAIKQVDVVISTVG 86
>At2g44040.1 68415.m05475 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae} ;
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 347
Score = 32.3 bits (70), Expect = 0.25
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +2
Query: 278 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYDAQRLKVCG 418
NGI V GC+G +G+ V G ++ + + D QR++VCG
Sbjct: 71 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCG 118
>At1g75300.1 68414.m08747 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 322
Score = 32.3 bits (70), Expect = 0.25
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +2
Query: 296 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 463
V G TG++G ++ K G R D ++ ++ DLG V L D
Sbjct: 10 VIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLG-VTILHGDLNDH 68
Query: 464 ESIAKAVRYSNVVINLVG 517
ES+ KA++ +VVI+ +G
Sbjct: 69 ESLVKAIKQVDVVISTIG 86
>At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 437
Score = 30.7 bits (66), Expect = 0.77
Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +2
Query: 269 SSFNGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 394
S+ NGI V G GFVG +V L + G +I L D+YD
Sbjct: 92 STNNGITVLVTGAAGFVGTHVSAALKRRGDGVIGLDNFNDYYD 134
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 29.5 bits (63), Expect = 1.8
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Frame = +2
Query: 176 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV---ATVFGCTGFVGRYVCNKLG 346
G+ S++++ + +Y+ R N + + GG FNG+V G G Y +
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLVDVYKKTLKSDGIAGLYRGFNIS 259
Query: 347 KIGTQLILPYRG---DFYDAQR-LKVCGDLGQVLFTPYHL 454
G I+ YRG YD+ + + + GDL F + L
Sbjct: 260 CAG---IIVYRGLYFGLYDSVKPVLLTGDLQDSFFASFAL 296
>At3g59890.2 68416.m06683 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 343
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 278 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 418
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 67 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 114
>At3g59890.1 68416.m06684 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 349
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 278 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 418
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 73 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 120
>At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SDR)
family protein similar to short-chain type
dehydrogenase/reductase SP:Q08632 [Picea abies]
Length = 270
Score = 29.1 bits (62), Expect = 2.3
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +2
Query: 281 GIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRL 406
G VA V G + +GR + L ++G ++++ Y DA+R+
Sbjct: 16 GRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 57
>At2g34070.1 68415.m04171 expressed protein
Length = 385
Score = 28.7 bits (61), Expect = 3.1
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +2
Query: 170 LNGS-MSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYV 331
L+G+ ++ Y+K + S K LAA GG+ G T GC F GR+V
Sbjct: 20 LSGADQALAYVKKPHVSQRNKTALAAVAGRGGGKEMLKGRKQT-SGCNLFQGRWV 73
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 4.1
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -1
Query: 287 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 201
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger)
family protein strong similarity to RING-H2 finger
protein RHA1a [Arabidopsis thaliana] GI:3790554;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 159
Score = 28.3 bits (60), Expect = 4.1
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = -1
Query: 452 DGKE*TGPGLNLRTLSTFGHHRNRLC-MVKLTGYQFFPI-CCTHSVRQIRCNRKLWLRCR 279
D E + P L LST N L +V+ + P CCT + + K+ +
Sbjct: 45 DHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPK 104
Query: 278 *SYCGHQYLAYMLQGWVYDPN 216
CGH + Y L W+ D N
Sbjct: 105 ---CGHVFHHYCLDRWIVDYN 122
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +2
Query: 311 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRY 490
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 491 SNVVIN 508
+++ N
Sbjct: 386 HDLIFN 391
>At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 434
Score = 27.9 bits (59), Expect = 5.4
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 278 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 394
NG+ V G GFVG +V L + G ++ L D+YD
Sbjct: 90 NGVSVLVTGAAGFVGTHVSAALKRRGDGVLGLDNFNDYYD 129
>At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong
similarity to pyruvate decarboxylase 1 [Vitis vinifera]
GI:10732644; contains InterPro entry IPR000399: Pyruvate
decarboxylase
Length = 607
Score = 27.1 bits (57), Expect = 9.4
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +2
Query: 293 TVFGCTGFVGRYVCNKLGKIGTQLILPYRGDF 388
TV C +GRY+ +L +IG + GDF
Sbjct: 39 TVSPCDATLGRYLARRLVEIGVTDVFSVPGDF 70
>At5g50310.1 68418.m06229 kelch repeat-containing protein similar to
Kelch repeats protein 3 (SP:Q08979) [Saccharomyces
cerevisiae]; contains Pfam PF01344: Kelch motif (6
repeats)
Length = 666
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 356 TQLILPYRGDFYDAQRLKVCGDL 424
T+LIL Y G+FY+ Q+ V GDL
Sbjct: 82 TELIL-YGGEFYNGQKTYVYGDL 103
>At5g44400.1 68418.m05436 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 537
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -1
Query: 359 GYQFFPICCTHSVRQIRCNRKLWLRCR*SYCGHQY--LAYMLQ 237
G+ F PI +H I C++KL + R GH Y L+Y+ Q
Sbjct: 85 GFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 127
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/68 (23%), Positives = 29/68 (42%)
Frame = +2
Query: 176 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLGKIG 355
G+M++ K + N A + G G + S N + TGF G ++G G
Sbjct: 506 GTMTMKRKKPCDAGERTSANCANNETGNGKKVSVNNVGEAEPADTGFTGLTDNLRIGSFG 565
Query: 356 TQLILPYR 379
++++ R
Sbjct: 566 NEVVIELR 573
>At4g19170.1 68417.m02829 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
9-cis-epoxycarotenoid dioxygenase [Phaseolus
vulgaris][GI:6715257]; neoxanthin cleavage enzyme,
Lycopersicon esculentum, PATX:E325797
Length = 595
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +2
Query: 443 PYHLLDEESIAKAVRYSNVVINLVGRDYETKNFKYN 550
PYHL D + + A++ N L R K +KYN
Sbjct: 156 PYHLFDGDGMLHAIKIHNGKATLCSR--YVKTYKYN 189
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,604,190
Number of Sequences: 28952
Number of extensions: 224470
Number of successful extensions: 513
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 486
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 513
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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