Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_H21
(612 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g08620.1 68416.m01001 KH domain-containing protein 62 2e-10
At2g38610.2 68415.m04743 KH domain-containing protein 62 2e-10
At2g38610.1 68415.m04742 KH domain-containing protein 62 2e-10
At5g56140.1 68418.m07003 KH domain-containing protein 62 4e-10
At1g09660.2 68414.m01085 KH domain-containing quaking protein, p... 60 1e-09
At1g09660.1 68414.m01084 KH domain-containing quaking protein, p... 60 1e-09
At4g26480.1 68417.m03810 KH domain-containing protein qkI-7, Mus... 60 2e-09
At5g51300.2 68418.m06360 splicing factor-related contains simila... 50 2e-06
At5g51300.1 68418.m06359 splicing factor-related contains simila... 50 2e-06
At3g32940.1 68416.m04174 expressed protein 33 0.11
At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.60
At3g04610.1 68416.m00493 KH domain-containing protein similar pu... 30 1.4
At1g22380.1 68414.m02799 UDP-glucoronosyl/UDP-glucosyl transfera... 29 1.8
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 29 2.4
At3g28270.2 68416.m03531 expressed protein similar to At14a prot... 29 2.4
At3g28270.1 68416.m03530 expressed protein similar to At14a prot... 29 2.4
At5g64390.2 68418.m08088 KH domain-containing RNA-binding protei... 29 3.2
At5g64390.1 68418.m08089 KH domain-containing RNA-binding protei... 29 3.2
At3g21810.1 68416.m02750 zinc finger (CCCH-type) family protein ... 29 3.2
At5g52280.1 68418.m06488 protein transport protein-related low s... 28 4.2
At5g16720.1 68418.m01958 expressed protein contains Pfam profile... 28 4.2
At5g24880.1 68418.m02946 expressed protein ; expression supporte... 27 7.4
At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protei... 27 7.4
At3g15670.1 68416.m01986 late embryogenesis abundant protein, pu... 27 7.4
At2g20616.1 68415.m02414 hypothetical protein 27 7.4
At1g22340.1 68414.m02795 UDP-glucoronosyl/UDP-glucosyl transfera... 27 9.8
>At3g08620.1 68416.m01001 KH domain-containing protein
Length = 283
Score = 62.5 bits (145), Expect = 2e-10
Identities = 23/47 (48%), Positives = 40/47 (85%)
Frame = +3
Query: 456 HPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
+P FNFVG+LLGP+GN++K+++ T C++ + G+GS++D +KEE+L+
Sbjct: 146 YPNFNFVGRLLGPRGNSLKRVEATTGCRVYIRGKGSIKDPEKEEKLK 192
>At2g38610.2 68415.m04743 KH domain-containing protein
Length = 286
Score = 62.5 bits (145), Expect = 2e-10
Identities = 23/47 (48%), Positives = 40/47 (85%)
Frame = +3
Query: 456 HPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
+P FNFVG+LLGP+GN++K+++ T C++ + G+GS++D +KE++LR
Sbjct: 147 YPNFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKGSIKDPEKEDKLR 193
>At2g38610.1 68415.m04742 KH domain-containing protein
Length = 286
Score = 62.5 bits (145), Expect = 2e-10
Identities = 23/47 (48%), Positives = 40/47 (85%)
Frame = +3
Query: 456 HPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
+P FNFVG+LLGP+GN++K+++ T C++ + G+GS++D +KE++LR
Sbjct: 147 YPNFNFVGRLLGPRGNSLKRVEATTGCRVFIRGKGSIKDPEKEDKLR 193
>At5g56140.1 68418.m07003 KH domain-containing protein
Length = 315
Score = 61.7 bits (143), Expect = 4e-10
Identities = 24/47 (51%), Positives = 38/47 (80%)
Frame = +3
Query: 456 HPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
+P FNFVG+LLGP+GN++K+++ T C++ + GRGS++D KEE +R
Sbjct: 177 YPNFNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPIKEEMMR 223
>At1g09660.2 68414.m01085 KH domain-containing quaking protein,
putative similar to GB:AAC67357
Length = 264
Score = 60.1 bits (139), Expect = 1e-09
Identities = 22/48 (45%), Positives = 40/48 (83%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
++P +NFVG++LGP+GN++K+++ T C++ + GRGS++D KEE+L+
Sbjct: 157 KYPSYNFVGRILGPRGNSLKRVELATHCRVFIRGRGSVKDTVKEEKLK 204
>At1g09660.1 68414.m01084 KH domain-containing quaking protein,
putative similar to GB:AAC67357
Length = 298
Score = 60.1 bits (139), Expect = 1e-09
Identities = 22/48 (45%), Positives = 40/48 (83%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
++P +NFVG++LGP+GN++K+++ T C++ + GRGS++D KEE+L+
Sbjct: 157 KYPSYNFVGRILGPRGNSLKRVELATHCRVFIRGRGSVKDTVKEEKLK 204
>At4g26480.1 68417.m03810 KH domain-containing protein qkI-7, Mus
musculus
Length = 555
Score = 59.7 bits (138), Expect = 2e-09
Identities = 22/48 (45%), Positives = 39/48 (81%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEELR 596
++P +NFVG+LLGP+GN++K+++ T C++ + GRGS++D KE+ +R
Sbjct: 417 KYPNYNFVGRLLGPRGNSLKRVEASTDCRVLIRGRGSIKDPIKEDMMR 464
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 49.6 bits (113), Expect = 2e-06
Identities = 16/46 (34%), Positives = 35/46 (76%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEE 590
E P +NF+G ++GP+GNT K+++ +T K+ + G+GS+++ + +++
Sbjct: 251 EFPGYNFIGLIIGPRGNTQKRMERETGAKIVIRGKGSVKEGRHQQK 296
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 49.6 bits (113), Expect = 2e-06
Identities = 16/46 (34%), Positives = 35/46 (76%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLGRGSMRDRQKEEE 590
E P +NF+G ++GP+GNT K+++ +T K+ + G+GS+++ + +++
Sbjct: 251 EFPGYNFIGLIIGPRGNTQKRMERETGAKIVIRGKGSVKEGRHQQK 296
>At3g32940.1 68416.m04174 expressed protein
Length = 607
Score = 33.5 bits (73), Expect = 0.11
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +3
Query: 453 EHPKFNFVGKLLGPKGNTMKQLQEDTLCKMAVLG 554
EH F+F+ + G +G+T K+L+++T K+ + G
Sbjct: 143 EHSDFSFLSLIFGSQGDTQKRLEKETGAKVQIFG 176
>At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 489
Score = 31.1 bits (67), Expect = 0.60
Identities = 20/50 (40%), Positives = 28/50 (56%)
Frame = +3
Query: 141 DKYDKNGYNSGDFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSEK 290
D++D GD KR + EL +GE+G K+ EKA E+ R L+EK
Sbjct: 415 DEWDVGIEIGGDVKREEVEAVVREL-MDGEKGKKMREKAVEWQR--LAEK 461
>At3g04610.1 68416.m00493 KH domain-containing protein similar
putative nucleic acid binding protein GB:CAB39665
[Arabidopsis thaliana]; Pfam HMM hit: KH domain family
of RNA binding proteins
Length = 577
Score = 29.9 bits (64), Expect = 1.4
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +3
Query: 477 GKLLGPKGNTMKQLQEDTLCKMAVLG 554
G L+G +G T+K +QE + C + VLG
Sbjct: 289 GSLIGKQGGTVKAIQEASACIVRVLG 314
>At1g22380.1 68414.m02799 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 467
Score = 29.5 bits (63), Expect = 1.8
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +3
Query: 171 GDFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSEKIKLNNSKFPLSMKLIDQE 344
GD KR + EL +GE+G K+ EKA E+ R L+EK +K P +I+ E
Sbjct: 403 GDVKRGEVEAVVREL-MDGEKGKKMREKAVEWRR--LAEKA----TKLPCGSSVINFE 453
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 29.1 bits (62), Expect = 2.4
Identities = 10/42 (23%), Positives = 20/42 (47%)
Frame = +1
Query: 379 RTQNMWMCSVTNQPKSQLKFLCQLRNILNSTLWENY*VQKET 504
+TQ +W C++ + F+C + +L + N + K T
Sbjct: 47 KTQGLWRCAICTYDNVETMFVCDICGVLRHPVAGNQSINKNT 88
>At3g28270.2 68416.m03531 expressed protein similar to At14a protein
(GI:11994571 and GI:11994573) [Arabidopsis thaliana]
Length = 374
Score = 29.1 bits (62), Expect = 2.4
Identities = 20/66 (30%), Positives = 33/66 (50%)
Frame = +3
Query: 201 KPEELDQNGEEGIKINEKAGEYMRELLSEKIKLNNSKFPLSMKLIDQEVTKVQASGRITK 380
K EE++++ E+GIK NE+A E + L+ + L ID E + +T+
Sbjct: 276 KVEEIEESVEKGIKTNEEATETVSILVDGLEDRIKNMLKLVDNAIDHEDNEAATRIVLTQ 335
Query: 381 DSKYVD 398
SK V+
Sbjct: 336 ISKKVE 341
>At3g28270.1 68416.m03530 expressed protein similar to At14a protein
(GI:11994571 and GI:11994573) [Arabidopsis thaliana]
Length = 374
Score = 29.1 bits (62), Expect = 2.4
Identities = 20/66 (30%), Positives = 33/66 (50%)
Frame = +3
Query: 201 KPEELDQNGEEGIKINEKAGEYMRELLSEKIKLNNSKFPLSMKLIDQEVTKVQASGRITK 380
K EE++++ E+GIK NE+A E + L+ + L ID E + +T+
Sbjct: 276 KVEEIEESVEKGIKTNEEATETVSILVDGLEDRIKNMLKLVDNAIDHEDNEAATRIVLTQ 335
Query: 381 DSKYVD 398
SK V+
Sbjct: 336 ISKKVE 341
>At5g64390.2 68418.m08088 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 824
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 474 VGKLLGPKGNTMKQLQEDTLCKMAV 548
VG ++G GN +KQLQ+ T K+ V
Sbjct: 58 VGAVIGKSGNVIKQLQQSTGAKIRV 82
>At5g64390.1 68418.m08089 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 857
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 474 VGKLLGPKGNTMKQLQEDTLCKMAV 548
VG ++G GN +KQLQ+ T K+ V
Sbjct: 58 VGAVIGKSGNVIKQLQQSTGAKIRV 82
>At3g21810.1 68416.m02750 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 437
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/37 (32%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +2
Query: 491 SKRKHY-EAITRRHTVQDGCTRQGLNERQAKRRRTPP 598
S++KHY + T + V+DG +G E+ ++ PP
Sbjct: 285 SRKKHYVDQYTTKEPVEDGLIGRGEEEKVENEKKRPP 321
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 28.3 bits (60), Expect = 4.2
Identities = 24/88 (27%), Positives = 44/88 (50%), Gaps = 2/88 (2%)
Frame = +3
Query: 126 LIKMADKYDKN-GYNSGDFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSEKIKLN 302
L++ A K +++ G +SG+ + +T + + E+LD + K NE+ + EL E L
Sbjct: 406 LLEEAKKLEEHKGMDSGNNEIDTLKQQIEDLDWELDSYKKKNEEQEILLDELTQEYESLK 465
Query: 303 NSKFP-LSMKLIDQEVTKVQASGRITKD 383
+ +S KL QE + + +KD
Sbjct: 466 EENYKNVSSKLEQQECSNAEDEYLDSKD 493
>At5g16720.1 68418.m01958 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 675
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +3
Query: 150 DKNGYNSGDFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSE 287
D+ Y G F+ +GK ++ QNGE I E G +RE+ E
Sbjct: 178 DRESYPLGFFRDKAEEGKKQDQQQNGEV-ISDVESYGLSLREVSEE 222
>At5g24880.1 68418.m02946 expressed protein ; expression supported
by MPSS
Length = 443
Score = 27.5 bits (58), Expect = 7.4
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +3
Query: 189 TTQGKPEELDQNGEEGIKINEKAGEYMRE 275
TTQ K EE+ + G+E ++ EK E ++E
Sbjct: 331 TTQEKEEEVKEEGKERVEEEEKEKEKVKE 359
>At5g22800.1 68418.m02666 aminoacyl-tRNA synthetase family protein
contains Pfam profiles: PF01411 tRNA synthetases class
II (A), PF02272 DHHA1 domain
Length = 978
Score = 27.5 bits (58), Expect = 7.4
Identities = 22/83 (26%), Positives = 37/83 (44%)
Frame = +3
Query: 150 DKNGYNSGDFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSEKIKLNNSKFPLSMK 329
D NG G F + K EL + +K+ KA + E+ E++ + K
Sbjct: 394 DMNGNLKGAFLPAVAE-KVIELSTYIDSDVKL--KASRIIEEIRQEELHFKKT-LERGEK 449
Query: 330 LIDQEVTKVQASGRITKDSKYVD 398
L+DQ++ + TKD+ Y+D
Sbjct: 450 LLDQKLNDALSIADKTKDTPYLD 472
>At3g15670.1 68416.m01986 late embryogenesis abundant protein,
putative / LEA protein, putative similar to SP|P13934
Late embryogenesis abundant protein 76 (LEA 76)
{Brassica napus}; contains Pfam profile PF02987: Late
embryogenesis abundant protein
Length = 225
Score = 27.5 bits (58), Expect = 7.4
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +3
Query: 189 TTQGKPEELDQNGEEGIKINEKAGEYMRELLSEKIK 296
TTQ E+ Q G++ + +KAG Y+ E E IK
Sbjct: 97 TTQAAKEKTSQAGDKAREAKDKAGSYLSE-TGEAIK 131
>At2g20616.1 68415.m02414 hypothetical protein
Length = 190
Score = 27.5 bits (58), Expect = 7.4
Identities = 21/73 (28%), Positives = 37/73 (50%), Gaps = 6/73 (8%)
Frame = +3
Query: 165 NSGDFKRNTTQGKPEELDQNGEEGIKIN------EKAGEYMRELLSEKIKLNNSKFPLSM 326
+S +RN+T + D G+K++ ++ + R +L EKIKL K L M
Sbjct: 30 SSSPNRRNSTSSSSTKRDDQNNNGLKVHLGLKKHDRMSDGTRLVLQEKIKL--QKKNLEM 87
Query: 327 KLIDQEVTKVQAS 365
K+ +++V+AS
Sbjct: 88 KINYVLLSQVKAS 100
>At1g22340.1 68414.m02795 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase; similar
to UDP-glucose glucosyltransferase GI:3928543 from
[Arabidopsis thaliana]
Length = 487
Score = 27.1 bits (57), Expect = 9.8
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +3
Query: 174 DFKRNTTQGKPEELDQNGEEGIKINEKAGEYMRELLSEKIKLNNSKFPLSMKLIDQEV 347
D KR + EL +GE+G K+ EKA E+ R L E + + ++++ + +V
Sbjct: 426 DVKREEVETVVREL-MDGEKGKKLREKAEEW-RRLAEEATRYKHGSSVMNLETLIHKV 481
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,615,154
Number of Sequences: 28952
Number of extensions: 215845
Number of successful extensions: 565
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 565
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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