BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_H09
(751 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59180.1 68418.m07417 DNA-directed RNA polymerase II identica... 202 2e-52
At4g14660.1 68417.m02254 RNA polymerase Rpb7 N-terminal domain-c... 90 2e-18
At3g22900.1 68416.m02886 RNA polymerase Rpb7 N-terminal domain-c... 79 3e-15
At1g06790.2 68414.m00722 RNA polymerase Rpb7 N-terminal domain-c... 50 2e-06
At1g06790.1 68414.m00723 RNA polymerase Rpb7 N-terminal domain-c... 50 2e-06
At4g14520.2 68417.m02238 DNA-directed RNA polymerase II-related ... 40 0.002
At4g14520.1 68417.m02237 DNA-directed RNA polymerase II-related ... 40 0.002
At5g60560.1 68418.m07593 F-box family protein contains Pfam prof... 30 1.4
At2g33040.1 68415.m04052 ATP synthase gamma chain, mitochondrial... 29 3.3
At1g70530.1 68414.m08117 protein kinase family protein contains ... 29 4.4
At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 5.8
At5g28770.2 68418.m03535 bZIP transcription factor family protei... 28 7.6
At5g28770.1 68418.m03534 bZIP transcription factor family protei... 28 7.6
>At5g59180.1 68418.m07417 DNA-directed RNA polymerase II identical
to Swiss-Prot:P38421 DNA-directed RNA polymerase II 19
kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
5) [Arabidopsis thaliana]
Length = 176
Score = 202 bits (493), Expect = 2e-52
Identities = 93/171 (54%), Positives = 125/171 (73%)
Frame = +1
Query: 163 MFYHISLEHXILLHPKYFGPQLLEXVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 342
MF+HI LE + LHP++FG L E + KL DVEGTC+G++GFV+A+T ID IG GLI+
Sbjct: 1 MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60
Query: 343 HGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMFAQIGPLKCFISHHSIPDYMEFC 522
G GFV +PVKY+ VVFRPFKGE+L+ +VT VNK+G FA+ GP++ F+S H IPD MEF
Sbjct: 61 DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120
Query: 523 PNVNPPCYKSKQEDSVIQEEDVIRLKIVGTRVDASGIFAIGTLIGDYLGLV 675
+ P Y + IQ+E +RLKI+GTRVDA+ IF +GT+ D+LG++
Sbjct: 121 AG-DMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAIFCVGTIKDDFLGVI 170
>At4g14660.1 68417.m02254 RNA polymerase Rpb7 N-terminal
domain-containing protein similar to SP|P52433
DNA-directed RNA polymerase II 19 kDa polypeptide (EC
2.7.7.6) (RPB7) {Rattus norvegicus}; contains Pfam
profile PF03876: RNA polymerase Rpb7, N-terminal domain
Length = 178
Score = 89.8 bits (213), Expect = 2e-18
Identities = 49/134 (36%), Positives = 82/134 (61%), Gaps = 3/134 (2%)
Frame = +1
Query: 277 TGKYGFVIAVTQIDNIGAGLIQHGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMF 456
T + G+ +AVT +D IG G I+ G V++PV + + F+ FKGE++ G+V +V K G+F
Sbjct: 43 TKELGYYVAVTTLDKIGEGKIREHTGEVLFPVMFSGMTFKIFKGEIIHGVVHKVLKHGVF 102
Query: 457 AQIGPLK-CFISHHSIPDYMEFCPNVNPPCYKSKQEDSVIQEEDVIRLKIVGTR-VDASG 630
+ GP++ ++S+ +PDY ++ P NP K S IQ E +R+ ++G + ++
Sbjct: 103 MRCGPIENVYLSYTKMPDY-KYIPGENPIFMNEK--TSRIQVETTVRVVVIGIKWMEVER 159
Query: 631 IF-AIGTLIGDYLG 669
F A+ +L GDYLG
Sbjct: 160 EFQALASLEGDYLG 173
>At3g22900.1 68416.m02886 RNA polymerase Rpb7 N-terminal
domain-containing protein weak similarity to SP|P52433
DNA-directed RNA polymerase II 19 kDa polypeptide (EC
2.7.7.6) (RPB7) {Rattus norvegicus}; contains Pfam
profile PF03876: RNA polymerase Rpb7, N-terminal domain
Length = 174
Score = 79.0 bits (186), Expect = 3e-15
Identities = 46/134 (34%), Positives = 74/134 (55%), Gaps = 3/134 (2%)
Frame = +1
Query: 277 TGKYGFVIAVTQIDNIGAGLIQHGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMF 456
T G++I T ++NIG G I+ G + +PV + + F+ FKGE++ G+V +V+K G+F
Sbjct: 42 TKDLGYLITPTILENIGEGKIKEQTGEIQFPVVFNGICFKMFKGEIVHGVVHKVHKTGVF 101
Query: 457 AQIGPLK-CFISHHSIPDYMEFCPNVNPPCYKSKQEDSVIQEEDVIRLKIVGT--RVDAS 627
+ GP + ++SH +P Y EF P NP + Q S IQ +R ++ T R
Sbjct: 102 LKSGPYEIIYLSHMKMPGY-EFIPGENP--FFMNQYMSRIQIGARVRFVVLDTEWREAEK 158
Query: 628 GIFAIGTLIGDYLG 669
A+ ++ GD LG
Sbjct: 159 DFMALASIDGDNLG 172
>At1g06790.2 68414.m00722 RNA polymerase Rpb7 N-terminal
domain-containing protein similar to SP|P35718
DNA-directed RNA polymerase III 25 kDa polypeptide (EC
2.7.7.6) {Saccharomyces cerevisiae}; contains Pfam
profile PF03876: RNA polymerase Rpb7, N-terminal domain
Length = 189
Score = 49.6 bits (113), Expect = 2e-06
Identities = 36/133 (27%), Positives = 54/133 (40%), Gaps = 1/133 (0%)
Frame = +1
Query: 163 MFYHISLEHXILLHPKYFGPQLLEXVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 342
MFY LEH + + P L + +K L G +++ I ++ G +
Sbjct: 1 MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60
Query: 343 HGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMFAQIGPLKCFISHHSIPDYMEFC 522
G G Y V + VVFRPF GEV+ + + G+ +G F +P +
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAAKFKESDANGLRLTLG----FFDDIYVPAPLMPK 116
Query: 523 PN-VNPPCYKSKQ 558
PN P Y KQ
Sbjct: 117 PNRCEPDPYNRKQ 129
>At1g06790.1 68414.m00723 RNA polymerase Rpb7 N-terminal
domain-containing protein similar to SP|P35718
DNA-directed RNA polymerase III 25 kDa polypeptide (EC
2.7.7.6) {Saccharomyces cerevisiae}; contains Pfam
profile PF03876: RNA polymerase Rpb7, N-terminal domain
Length = 204
Score = 49.6 bits (113), Expect = 2e-06
Identities = 36/133 (27%), Positives = 54/133 (40%), Gaps = 1/133 (0%)
Frame = +1
Query: 163 MFYHISLEHXILLHPKYFGPQLLEXVKQKLYTDVEGTCTGKYGFVIAVTQIDNIGAGLIQ 342
MFY LEH + + P L + +K L G +++ I ++ G +
Sbjct: 1 MFYLSELEHSLRVPPHLLNLPLEDAIKSVLQNVFLDKVLADLGLCVSIYDIKSVEGGFVL 60
Query: 343 HGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMFAQIGPLKCFISHHSIPDYMEFC 522
G G Y V + VVFRPF GEV+ + + G+ +G F +P +
Sbjct: 61 PGDGAATYKVGLRIVVFRPFVGEVIAAKFKESDANGLRLTLG----FFDDIYVPAPLMPK 116
Query: 523 PN-VNPPCYKSKQ 558
PN P Y KQ
Sbjct: 117 PNRCEPDPYNRKQ 129
>At4g14520.2 68417.m02238 DNA-directed RNA polymerase II-related
contains similarity to Swiss-Prot:P38421 DNA-directed
RNA polymerase II 19 kDa polypeptide (EC 2.7.7.6) (RNA
polymerase II subunit 5) [Arabidopsis thaliana]
Length = 200
Score = 39.9 bits (89), Expect = 0.002
Identities = 31/127 (24%), Positives = 58/127 (45%), Gaps = 11/127 (8%)
Frame = +1
Query: 163 MFYHISLEHXILLHPKYF-GPQLLEXVKQKLYTDV--EGTCTGKYGFVIAVTQIDNIGAG 333
MF + + + + K+ G + + +L D+ E C ++GF + +T + +IG
Sbjct: 1 MFSEVEMARDVAICAKHLNGQSPHQPILCRLLQDLIHEKACR-EHGFYLGITALKSIGNN 59
Query: 334 LIQ-------HGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMFAQIGPLK-CFIS 489
H + +PV + F P +G++L G V +V G F + GPL+ ++S
Sbjct: 60 KNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYLS 119
Query: 490 HHSIPDY 510
+P Y
Sbjct: 120 LLKMPHY 126
>At4g14520.1 68417.m02237 DNA-directed RNA polymerase II-related
contains similarity to Swiss-Prot:P38421 DNA-directed
RNA polymerase II 19 kDa polypeptide (EC 2.7.7.6) (RNA
polymerase II subunit 5) [Arabidopsis thaliana]
Length = 200
Score = 39.9 bits (89), Expect = 0.002
Identities = 31/127 (24%), Positives = 58/127 (45%), Gaps = 11/127 (8%)
Frame = +1
Query: 163 MFYHISLEHXILLHPKYF-GPQLLEXVKQKLYTDV--EGTCTGKYGFVIAVTQIDNIGAG 333
MF + + + + K+ G + + +L D+ E C ++GF + +T + +IG
Sbjct: 1 MFSEVEMARDVAICAKHLNGQSPHQPILCRLLQDLIHEKACR-EHGFYLGITALKSIGNN 59
Query: 334 LIQ-------HGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVNKVGMFAQIGPLK-CFIS 489
H + +PV + F P +G++L G V +V G F + GPL+ ++S
Sbjct: 60 KNNNIDNENNHQAKILTFPVSFTCRTFLPARGDILQGTVKKVLWNGAFIRSGPLRYAYLS 119
Query: 490 HHSIPDY 510
+P Y
Sbjct: 120 LLKMPHY 126
>At5g60560.1 68418.m07593 F-box family protein contains Pfam
profile: PF00646 F-box domain
Length = 374
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/50 (28%), Positives = 22/50 (44%)
Frame = +2
Query: 32 NLVSNLQFACSVARCCCFCLFLKNHNXTNLIAWXPHRNLSTAEKCSTTFH 181
NL+ L C V +C L + T L+ W P+ KC+ ++H
Sbjct: 91 NLLDQLDI-CKVFQCDGLLLCVTKEENTRLVVWNPYSGQIRWIKCNNSYH 139
>At2g33040.1 68415.m04052 ATP synthase gamma chain, mitochondrial
(ATPC) identical to SP|Q96250 ATP synthase gamma chain,
mitochondrial precursor (EC 3.6.3.14) {Arabidopsis
thaliana}; contains Pfam profile: PF00231 ATP synthase
Length = 325
Score = 29.1 bits (62), Expect = 3.3
Identities = 23/72 (31%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
Frame = +1
Query: 265 EGTCTGKYGFVIAVTQ-IDNIGAGLIQHGQGFVMYPVKYKAVVFRPFKGEVLDGIVTQVN 441
+G C G V+ V++ + + AG + Q FV+ K KA++FR K +++ VT++N
Sbjct: 114 KGLCGGINSTVVKVSRALYKLNAGPEKEVQ-FVIVGEKAKAIMFRDSKNDIVLS-VTELN 171
Query: 442 KVGM-FAQIGPL 474
K + +AQ+ L
Sbjct: 172 KNPLNYAQVSVL 183
>At1g70530.1 68414.m08117 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 646
Score = 28.7 bits (61), Expect = 4.4
Identities = 19/54 (35%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +2
Query: 107 NXTNLIAWXPHRNLSTAEKCSTTFHWS-TXFCYIRNISVHNYWXQSNRSYTLMW 265
N NLI+ H+NL CS T S + YI N S+H+Y L W
Sbjct: 366 NEVNLISQVDHKNLVKLLGCSITGPESLLVYEYIANQSLHDYLFVRKDVQPLNW 419
>At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to flavonol synthase SP|Q96330
{Arabidopsis thaliana}, SP|Q07512 {Petunia hybrida};
contains Pfam profile PF03171: oxidoreductase,
2OG-Fe(II) oxygenase family
Length = 357
Score = 28.3 bits (60), Expect = 5.8
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = +1
Query: 481 FISHHSIP--DYMEFCPNVNPPCYKSK 555
FI H+S P +++ P+ NPPCYK K
Sbjct: 144 FIKHYSHPLSKWIDMWPS-NPPCYKDK 169
>At5g28770.2 68418.m03535 bZIP transcription factor family protein
similar to seed storage protein opaque-2(bZIP
family)GI:168428 from Zea mays; contains Pfam profile
PF00170: bZIP transcription factor; identical to cDNA
bZIP protein BZO2H3 GI:10954098
Length = 314
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = -3
Query: 485 IKHLRG--PICANMPTLFTCVTIPSKTSPLNGLNTTALYLT 369
+K L G P+ NMP + + V++PS+TS N +TT+ +T
Sbjct: 232 VKRLTGFNPMFHNMPQIVSTVSLPSETS--NSPDTTSSQVT 270
>At5g28770.1 68418.m03534 bZIP transcription factor family protein
similar to seed storage protein opaque-2(bZIP
family)GI:168428 from Zea mays; contains Pfam profile
PF00170: bZIP transcription factor; identical to cDNA
bZIP protein BZO2H3 GI:10954098
Length = 307
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = -3
Query: 485 IKHLRG--PICANMPTLFTCVTIPSKTSPLNGLNTTALYLT 369
+K L G P+ NMP + + V++PS+TS N +TT+ +T
Sbjct: 225 VKRLTGFNPMFHNMPQIVSTVSLPSETS--NSPDTTSSQVT 263
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,961,838
Number of Sequences: 28952
Number of extensions: 333912
Number of successful extensions: 819
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 817
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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