Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_H01
(716 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 285 3e-77
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 283 8e-77
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 260 5e-70
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 260 9e-70
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 169 2e-42
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 165 3e-41
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 161 3e-40
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 3.1
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 3.1
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 3.1
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 4.1
At4g04710.1 68417.m00692 calcium-dependent protein kinase, putat... 28 7.1
At3g63330.1 68416.m07125 protein kinase family protein contains ... 27 9.4
At1g21440.1 68414.m02681 mutase family protein similar to carbox... 27 9.4
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 285 bits (698), Expect = 3e-77
Identities = 137/213 (64%), Positives = 159/213 (74%), Gaps = 2/213 (0%)
Frame = +3
Query: 84 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 257
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 258 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 437
D L +NG K+ V S+RDP IPW GA+YVVES+GVFTT KA++HL+GGAKKVIIS
Sbjct: 147 DDSTLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIIS 206
Query: 438 APSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXX 617
APSADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 207 APSADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATT 266
Query: 618 XXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
DGPS K WR GRGA QNI P+STGAAK
Sbjct: 267 ATQKTVDGPSMKDWRGGRGASQNIIPSSTGAAK 299
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 283 bits (694), Expect = 8e-77
Identities = 136/213 (63%), Positives = 160/213 (75%), Gaps = 2/213 (0%)
Frame = +3
Query: 84 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 257
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 258 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 437
D L +NG ++ V S+RDP IPW GAEYVVES+GVFTT +AS+HL+GGAKKVIIS
Sbjct: 145 DDSTLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIIS 204
Query: 438 APSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXX 617
APSADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGLM
Sbjct: 205 APSADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGLMTTVHATT 264
Query: 618 XXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
DGPS K WR GRGA QNI P+STGAAK
Sbjct: 265 ATQKTVDGPSMKDWRGGRGASQNIIPSSTGAAK 297
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 260 bits (638), Expect = 5e-70
Identities = 129/219 (58%), Positives = 160/219 (73%), Gaps = 3/219 (1%)
Frame = +3
Query: 69 LAENMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFKG 245
+A+ +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 246 -SVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 419
++V+D ++ G K + VF R+P+ IPWG+AGA++VVESTGVFT DKA+AHL+GGA
Sbjct: 61 HELKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGA 120
Query: 420 KKVIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMX 599
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMT 180
Query: 600 XXXXXXXXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
DGPS K WR GR A NI P+STGAAK
Sbjct: 181 TVHSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAK 219
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 260 bits (636), Expect = 9e-70
Identities = 128/219 (58%), Positives = 159/219 (72%), Gaps = 3/219 (1%)
Frame = +3
Query: 69 LAENMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 242
+A+ +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 243 GSVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 419
++++D ++ G K + VF R+P+ IPW +AGA+YVVESTGVFT DKA+AHL+GGA
Sbjct: 61 NELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGA 120
Query: 420 KKVIISAPSADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMX 599
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGLM
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMT 180
Query: 600 XXXXXXXXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
DGPS K WR GR A NI P+STGAAK
Sbjct: 181 TVHSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAK 219
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 169 bits (410), Expect = 2e-42
Identities = 99/221 (44%), Positives = 121/221 (54%), Gaps = 5/221 (2%)
Frame = +3
Query: 69 LAENMSKIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRF 239
+ E K+ INGFGRIGR LR + VV IND G+ +L KYDST G F
Sbjct: 60 VTEAKIKVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIF 118
Query: 240 KGSVEVQ-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGG 416
V+ D L V+G I + S+R+P +PWG+ G + V+E TGVF D A HL+ G
Sbjct: 119 DADVKPSGDSALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAG 178
Query: 417 AKKVIISAP-SADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 593
AKKV+I+AP D P +VVGVN Y +ISNASCTTNCLAP KV+ F I++G
Sbjct: 179 AKKVLITAPGKGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVLDQKFGIIKGT 238
Query: 594 MXXXXXXXXXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
M D L R R A NI P STGAAK
Sbjct: 239 MTTTHSYTGDQRLLDASHRDL-RRARAAALNIVPTSTGAAK 278
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 165 bits (400), Expect = 3e-41
Identities = 97/221 (43%), Positives = 121/221 (54%), Gaps = 5/221 (2%)
Frame = +3
Query: 69 LAENMSKIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRF 239
+ E K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F
Sbjct: 57 VTEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIF 115
Query: 240 KGSVEVQ-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGG 416
V+ + + V+G I V S R+P +PW + G + V+E TGVF + A H+E G
Sbjct: 116 DADVKPSGETAISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAG 175
Query: 417 AKKVIISAPS-ADAPMFVVGVNLXAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 593
AKKVII+AP D P +VVGVN AY +ISNASCTTNCLAP KV+ F I++G
Sbjct: 176 AKKVIITAPGKGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQKFGIIKGT 235
Query: 594 MXXXXXXXXXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
M D L R R A NI P STGAAK
Sbjct: 236 MTTTHSYTGDQRLLDASHRDL-RRARAAALNIVPTSTGAAK 275
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 161 bits (392), Expect = 3e-40
Identities = 97/217 (44%), Positives = 121/217 (55%), Gaps = 7/217 (3%)
Frame = +3
Query: 87 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 257
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK V++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 258 QDGFLV-VNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 434
D + V+G I V S RDP +PW + G + V+E TGVF A H++ GA KVII
Sbjct: 142 VDNETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVII 201
Query: 435 SAPS--ADAPMFVVGVNLXAYDPSF-KVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXX 605
+AP+ AD P +V+GVN Y +ISNASCTTNCLAP AKV+ + F IV+G M
Sbjct: 202 TAPAKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVLDEEFGIVKGTMTTT 261
Query: 606 XXXXXXXXXXDGPSGKLWRDGRGAQQNIXPASTGAAK 716
D L R R A NI P STGAAK
Sbjct: 262 HSYTGDQRLLDASHRDL-RRARAAALNIVPTSTGAAK 297
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 3.1
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 96 INGFGRIGRLVLRASIEKGAQVVAIND 176
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 3.1
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 96 INGFGRIGRLVLRASIEKGAQVVAIND 176
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -1
Query: 197 IVKTDERVIYSDHLSSFFNRSTEHQTANAAKS 102
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 4.1
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3
Query: 87 KIGINGFGRIGRLVLRASIEKGAQVVAIND 176
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
>At4g04710.1 68417.m00692 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 575
Score = 27.9 bits (59), Expect = 7.1
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +2
Query: 11 LHLVLNVRGGGQLFNKFSITCREH 82
+H+V+ + GGG+LF+K + H
Sbjct: 96 IHIVMELCGGGELFDKIDALVKSH 119
>At3g63330.1 68416.m07125 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.5 bits (58), Expect = 9.4
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 370 VSLPLQIKHLLTWREVLKKLLYQLPVLMPPCLL 468
V+ L +H+ W E K+L Y+L LM C+L
Sbjct: 262 VTRALLDQHIRGWSENFKELAYKLRSLMEMCIL 294
>At1g21440.1 68414.m02681 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 336
Score = 27.5 bits (58), Expect = 9.4
Identities = 13/52 (25%), Positives = 23/52 (44%)
Frame = +3
Query: 336 KAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLXAY 491
+ A + TG+ D+A+ ++E GA + AP D + +G Y
Sbjct: 191 RTDARALSAKTGLSDAIDRANLYMEAGADASFVEAPRDDDELKEIGRRTKGY 242
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,219,271
Number of Sequences: 28952
Number of extensions: 308652
Number of successful extensions: 916
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 896
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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