BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_G21 (557 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 86 3e-18 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 50 2e-07 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 47 2e-06 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 44 2e-05 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.028 SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 31 0.15 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 30 0.27 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 29 0.35 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.9 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 4.3 SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma... 25 5.7 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 5.7 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 25 5.7 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 25 7.5 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 86.2 bits (204), Expect = 3e-18 Identities = 40/117 (34%), Positives = 70/117 (59%) Frame = +1 Query: 184 ILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRL 363 ++R +GQ L+++I EVDAD +G ++F EF+T+ A+ + + D E +E+REAF++ Sbjct: 37 VMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNE---EEVREAFKV 93 Query: 364 YDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFMEMMTGE 534 +DK+GNGYI L + I+E DTDG G ++++EF +++ + Sbjct: 94 FDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISSK 150 Score = 50.0 bits (114), Expect = 2e-07 Identities = 23/62 (37%), Positives = 34/62 (54%) Frame = +1 Query: 340 ELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFME 519 E REAF L+D++ +G I ++ L + I E+D DG+GT+DF EF+ Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72 Query: 520 MM 525 MM Sbjct: 73 MM 74 Score = 26.2 bits (55), Expect = 3.3 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +3 Query: 90 LPPEQIAVLRKAFDGFDHNRSGSI 161 L EQIA R+AF FD ++ G+I Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNI 29 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 50.0 bits (114), Expect = 2e-07 Identities = 30/116 (25%), Positives = 53/116 (45%) Frame = +1 Query: 187 LRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLY 366 +R +G K + +++ + D G L+ +FV + + IVE D +E++ AF L+ Sbjct: 63 MRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDP---LEEIKRAFELF 119 Query: 367 DKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFMEMMTGE 534 D + G I +LR + I+E D D G ++ EF+ +M E Sbjct: 120 DDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIMMDE 175 Score = 33.9 bits (74), Expect = 0.016 Identities = 16/71 (22%), Positives = 32/71 (45%) Frame = +1 Query: 316 EDAEAMQKELREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGT 495 E E ++++ EAF+L+D + + I LR +++ D G G Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89 Query: 496 VDFDEFMEMMT 528 + ++F+ +MT Sbjct: 90 LQMEDFVRVMT 100 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 47.2 bits (107), Expect = 2e-06 Identities = 29/109 (26%), Positives = 50/109 (45%), Gaps = 5/109 (4%) Frame = +1 Query: 220 ILEELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTS 399 + L VD D G ++F EF+ + F V + E ++L+ AF++YD + +GYI Sbjct: 57 LASRLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNG 113 Query: 400 SL-----REIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFMEMMTG 531 L + I E+D D G + F+EF ++++G Sbjct: 114 ELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSG 162 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 43.6 bits (98), Expect = 2e-05 Identities = 28/119 (23%), Positives = 55/119 (46%) Frame = +1 Query: 163 PXDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKE 342 P V +LR +G L +L E+ ++ +F++ + + E ++E +E Sbjct: 27 PTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSFVSNKLRETESE---EE 79 Query: 343 LREAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFME 519 +AFR++DK+ +GYI T+ + +QE D SG+ D+ +F++ Sbjct: 80 YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138 Score = 29.1 bits (62), Expect = 0.46 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = +1 Query: 340 ELREAFRLYDKEGNGYIPTSSLREI 414 E++EAF LYD + +G IPTS + + Sbjct: 10 EMKEAFVLYDIDKDGLIPTSHVGSV 34 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 33.1 bits (72), Expect = 0.028 Identities = 21/63 (33%), Positives = 32/63 (50%) Frame = +1 Query: 226 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 405 E + DADK+G ++F EF+ + V E + +L AF+LYD + NG I + Sbjct: 66 EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLISYDEM 121 Query: 406 REI 414 I Sbjct: 122 LRI 124 >SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 2280 Score = 30.7 bits (66), Expect = 0.15 Identities = 17/60 (28%), Positives = 31/60 (51%) Frame = -3 Query: 501 VNSAASVSVDLLDEAIEFIVSQLLVQFPEDLAQARRGNVTVAFLVVQSERFPEFFLHGLS 322 VN A + + DL D AI ++Q+L F +DL + VT+ + +P ++ + +S Sbjct: 1251 VNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYYTYRVS 1310 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 29.9 bits (64), Expect = 0.27 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = +1 Query: 217 KILEELIEEVDADKSGRLEFPEF 285 + LEE ++ DAD SG+L F EF Sbjct: 333 EFLEETFQKADADHSGKLSFEEF 355 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 29.5 bits (63), Expect = 0.35 Identities = 15/60 (25%), Positives = 31/60 (51%) Frame = +1 Query: 346 REAFRLYDKEGNGYIPTSSLREIXXXXXXXXXXXXXXXXIQEIDTDGSGTVDFDEFMEMM 525 ++AF L+D+ G G IP +S+ ++ I EI++ VD ++F++++ Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDL----LRACGQNPTLAEITEIESTLPAEVDMEQFLQVL 64 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 27.1 bits (57), Expect = 1.9 Identities = 11/18 (61%), Positives = 15/18 (83%) Frame = +1 Query: 337 KELREAFRLYDKEGNGYI 390 +EL+EAF L DK+G+G I Sbjct: 48 QELKEAFALLDKDGDGNI 65 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 25.8 bits (54), Expect = 4.3 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = +1 Query: 331 MQKELREAFRLYDKEGNGYIPTSSLR 408 M +E EAF L+D GYI LR Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35 >SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 535 Score = 25.4 bits (53), Expect = 5.7 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -2 Query: 208 MAAPSFSGYQPRSXQGILPERL 143 + PSFS Y+ +S Q +LP+ L Sbjct: 447 LTGPSFSRYELQSAQSVLPKDL 468 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 25.4 bits (53), Expect = 5.7 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +1 Query: 223 LEELIEEVDADKSGRLEFPEFV 288 + E I EV+ D SGR+E +FV Sbjct: 56 VREAIREVNVDSSGRVEPEDFV 77 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 25.4 bits (53), Expect = 5.7 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = -2 Query: 61 TMVDVTS*HCETHNTTHRP 5 T++D+ S HCE N RP Sbjct: 1099 TLIDIGSIHCEISNFARRP 1117 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 25.0 bits (52), Expect = 7.5 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = +1 Query: 277 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEG 378 PEF AA+ +VEED E + EA + D+EG Sbjct: 38 PEFSDEAAEALVEEDIEDLD---GEALDVEDEEG 68 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,818,897 Number of Sequences: 5004 Number of extensions: 30159 Number of successful extensions: 121 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 114 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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