Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P01_F_F01
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 33 0.13
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.39
At3g49990.1 68416.m05466 expressed protein 32 0.39
At5g63550.1 68418.m07976 expressed protein 31 0.90
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 30 1.2
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 2.1
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 27 2.5
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 3.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 29 3.6
At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 4.8
At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide famil... 28 6.4
At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide famil... 28 6.4
At2g02170.1 68415.m00153 remorin family protein contains Pfam do... 28 6.4
At1g12080.2 68414.m01397 expressed protein 28 6.4
At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein ... 27 8.4
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 33.5 bits (73), Expect = 0.13
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +3
Query: 432 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 584
P A AK+ +K ++ ++ +DS +E + +K KP+A D+ S D +
Sbjct: 141 PAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKIAKPAAKDSSSSDDDS 191
Score = 27.5 bits (58), Expect = 8.4
Identities = 19/81 (23%), Positives = 35/81 (43%)
Frame = +3
Query: 324 AKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKVEEPAAQPEDSKT 503
+ DD + EDS+ + S D++ +S D E+PA + D+K
Sbjct: 185 SSSDDDSDEDSEDEKPATKKAAPAAAKAASSSDSSDEDSDEESED---EKPAQKKADTKA 241
Query: 504 EVQATVAEISKEEKPSATDAE 566
+++ E S+ E+ + D E
Sbjct: 242 SKKSSSDESSESEEDESEDEE 262
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 31.9 bits (69), Expect = 0.39
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -1
Query: 238 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 134
+T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 31.9 bits (69), Expect = 0.39
Identities = 23/73 (31%), Positives = 33/73 (45%)
Frame = +3
Query: 429 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMVK 608
+P A+ D+K E P A+P KT Q + E K+E+ +A AE A II K
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE--KKERKNAVKAE--KREARIIKKQTK 477
Query: 609 KIDLAPTVXSDAA 647
+ T + A
Sbjct: 478 MLYCGETQRAQRA 490
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 30.7 bits (66), Expect = 0.90
Identities = 18/67 (26%), Positives = 34/67 (50%)
Frame = +3
Query: 426 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 605
E D + + +++VE+P+ + SK V+ + K+++PS A+GSA S +
Sbjct: 320 EKTDTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPS---AKGSARSGEKSSKQI 376
Query: 606 KKIDLAP 626
K +P
Sbjct: 377 AKSTSSP 383
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 30.3 bits (65), Expect = 1.2
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +3
Query: 432 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSA 554
P E K + K EE + E+ KTE ++ AE +EEK +A
Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEKSAA 163
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 29.5 bits (63), Expect = 2.1
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +3
Query: 303 EVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKV-EEPA 479
E P + E KKD+ AP ++ + K E+ + + + A + V EE
Sbjct: 73 ETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKTEEAPVVVEEEKK 129
Query: 480 AQPEDSKTEVQATV-AEISKEEK 545
+ E+ K V+A+V A + K ++
Sbjct: 130 PEAEEEKPAVEASVTAPVEKADE 152
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 27.1 bits (57), Expect(2) = 2.5
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +3
Query: 426 EIPDAEAKSADIKVEEPAAQPED-SKTEVQATVAEISKEEKPSATDAE 566
E+P E+K ++ V+ +A+PE S+++ + T E + E KP T AE
Sbjct: 109 EVPPEESKR-EVVVQPESAKPETKSESKPETTKPETTSETKPE-TKAE 154
Score = 20.6 bits (41), Expect(2) = 2.5
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +3
Query: 315 IPEAKKDDIAPEDS 356
+PE K ++ PE+S
Sbjct: 102 VPEESKQEVPPEES 115
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 3.6
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3
Query: 300 DEVPAIPEAKKDDIAPE 350
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.7 bits (61), Expect = 3.6
Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 7/72 (9%)
Frame = +3
Query: 414 AKSSEIPDAEAKSADIKVEEPAAQPED-------SKTEVQATVAEISKEEKPSATDAEGS 572
A E P AE ++ VEE AAQPE + E + A K++K + E
Sbjct: 308 AALGETPAAERPASSTPVEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKK 367
Query: 573 ADSAAIIPNMVK 608
A +AA + V+
Sbjct: 368 AAAAAAATSSVE 379
>At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 793
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 436 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 549
M K+N+ + K+K ++L LK K YK + K Q + L
Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777
>At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.9 bits (59), Expect = 6.4
Identities = 23/78 (29%), Positives = 34/78 (43%)
Frame = +3
Query: 426 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 605
E+ E ++K EEPA E +KTE T E KEE T +G A++
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTE--GTSGE--KEEIVEET-KKGETPETAVVEEKK 187
Query: 606 KKIDLAPTVXSDAAAVPE 659
+++ + A AV E
Sbjct: 188 PEVEEKKEEATPAPAVVE 205
>At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.9 bits (59), Expect = 6.4
Identities = 23/78 (29%), Positives = 34/78 (43%)
Frame = +3
Query: 426 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPNMV 605
E+ E ++K EEPA E +KTE T E KEE T +G A++
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTE--GTSGE--KEEIVEET-KKGETPETAVVEEKK 187
Query: 606 KKIDLAPTVXSDAAAVPE 659
+++ + A AV E
Sbjct: 188 PEVEEKKEEATPAPAVVE 205
>At2g02170.1 68415.m00153 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 486
Score = 27.9 bits (59), Expect = 6.4
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 435 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 584
+AE K ++KVE + +D + AT+ E EEK +A +A+ +A
Sbjct: 411 EAEMKKTEVKVERIKGRAQDRLMKKLATI-ERKAEEKRAAAEAKKDHQAA 459
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 27.9 bits (59), Expect = 6.4
Identities = 21/81 (25%), Positives = 39/81 (48%)
Frame = +3
Query: 420 SSEIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPN 599
+ E+ EA+ D + E+ + +D KTEV + +EEK AE ++ A++
Sbjct: 57 NEEVVVEEAEKKDEETEKKTEE-KDEKTEVITETPVVEEEEK----KAEEVTETPAVVEE 111
Query: 600 MVKKIDLAPTVXSDAAAVPEI 662
KK ++ ++ AA E+
Sbjct: 112 -EKKTEVVEEKQTEVAAAEEV 131
>At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 586
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -2
Query: 402 FLRFSLQERFPVSRYRSLLGLYRPFWPLV 316
FL F E PV ++ L L RP WPL+
Sbjct: 25 FLSFPRPENTPVQLFQLLHTLERPGWPLL 53
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,407,336
Number of Sequences: 28952
Number of extensions: 158862
Number of successful extensions: 501
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 491
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 501
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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