BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_E17 (589 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 74 2e-14 SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 56 5e-09 SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 34 0.018 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 0.88 SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 28 1.2 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 4.7 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 73.7 bits (173), Expect = 2e-14 Identities = 48/166 (28%), Positives = 85/166 (51%), Gaps = 4/166 (2%) Frame = +2 Query: 71 FPAGKVPAFXSADGKVLLTESNAIAYYVANXS----LRGGXLATQARVWQWASWSDSELL 238 FP K+P F DG L+E+ AIA+Y+A+ + L G +A+V Q+ S+++SEL Sbjct: 47 FPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQYCSFTNSELP 105 Query: 239 PASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFST 418 A + P + +++Q + A++ + D L ++T+LV R+TLAD+ Sbjct: 106 GAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLTLADIFFTCF 165 Query: 419 LLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAP 556 L +VL S + ++ R++ T+ HQ ++ A+ L P Sbjct: 166 LKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFIDQP 211 >SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 220 Score = 55.6 bits (128), Expect = 5e-09 Identities = 39/157 (24%), Positives = 78/157 (49%), Gaps = 8/157 (5%) Frame = +2 Query: 71 FPAGKVPAFXSADGKVLLTESNAIAYYVA----NXSLRGGXL--ATQARVWQWASWSDSE 232 FP K+P F ADG L + Y+ N G + +A + +W + + + Sbjct: 47 FPLQKLPVFIGADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFD 106 Query: 233 LL-PASCA-WVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVI 406 ++ P + WV + G + + ++ + + + + +LK+ + + RT+LV +R TLAD+ Sbjct: 107 IVTPQNVRPWVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLF 166 Query: 407 VFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 517 S L F ++D R L ++ R+++T+ HQ ++ Sbjct: 167 FGSLLRIFFNSIIDEKTRKELPHLTRYYITMFHQAKL 203 >SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 33.9 bits (74), Expect = 0.018 Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%) Frame = +2 Query: 341 LDGHLLTRTFLVTER-ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 517 LD L + TF+ + I++AD+ V++ + H++ L L NV RWF + HQ V Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186 Query: 518 SAVVGSLTL 544 S+++ Sbjct: 187 MEAANSMSM 195 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 28.3 bits (60), Expect = 0.88 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = +3 Query: 318 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 410 TY PP Y D+FS APS+ + S+S Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287 >SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 27.9 bits (59), Expect = 1.2 Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%) Frame = +3 Query: 180 LWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 275 LW+P PV SG T C+ LP G +T V Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 25.8 bits (54), Expect = 4.7 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Frame = +3 Query: 210 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 317 GH L + C P++GSS L+ +C N R+ V++L Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,310,535 Number of Sequences: 5004 Number of extensions: 44076 Number of successful extensions: 136 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 254167452 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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