BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_E17 (589 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 101 3e-22 At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 96 1e-20 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 42 4e-04 At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 37 0.011 At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 34 0.061 At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 34 0.081 At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 34 0.081 At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 33 0.11 At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 32 0.25 At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive de... 32 0.33 At1g16840.2 68414.m02028 expressed protein 31 0.43 At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 31 0.43 At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 31 0.43 At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 1.7 At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 29 3.0 At3g49210.1 68416.m05378 expressed protein 29 3.0 At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 3.0 At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta... 28 5.3 At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 28 5.3 At1g47220.1 68414.m05227 cyclin, putative similar to cyclin A-li... 27 9.3 At1g16840.4 68414.m02027 expressed protein 27 9.3 At1g16840.3 68414.m02026 expressed protein 27 9.3 At1g16840.1 68414.m02025 expressed protein 27 9.3 >At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / eEF-1B gamma, putative Similar to elongation factor 1-gamma (gb|EF1G_XENLA). ESTs gb|T20564,gb|T45940,gb|T04527 come from this gene Length = 414 Score = 101 bits (243), Expect = 3e-22 Identities = 63/172 (36%), Positives = 87/172 (50%), Gaps = 4/172 (2%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVA----NXSLRGGXLATQARVWQWASWSDSELLP 241 P GKVP + +G V ESNAIA YV+ + SL G L A++ QW +S E+ Sbjct: 50 PIGKVPVLETPEGSVF--ESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYA 107 Query: 242 ASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTL 421 + W P +G M ++ E A S L AL L+ HL + T+LV ITLAD+I L Sbjct: 108 SILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNL 167 Query: 422 LHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYXPKK 577 F V+ S +V+R+F TV +QP + V+G + A P KK Sbjct: 168 NLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIASKK 219 >At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / eEF-1B gamma, putative similar to elongation factor 1B gamma GI:3868758 from [Oryza sativa] Length = 413 Score = 96.3 bits (229), Expect = 1e-20 Identities = 59/172 (34%), Positives = 84/172 (48%), Gaps = 4/172 (2%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVA----NXSLRGGXLATQARVWQWASWSDSELLP 241 P GKVP + +G + ESNAIA YV+ + SL G L A + QW +S E+ Sbjct: 50 PIGKVPVLETPEGPIF--ESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDA 107 Query: 242 ASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTL 421 W P +G F+ E A S L L+ L+ HL + TFLV +TLAD++ L Sbjct: 108 NMLKWFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNL 167 Query: 422 LHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYXPKK 577 F V+ S+ +V+R+F T+ +QP+ V+G A P KK Sbjct: 168 NLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKK 219 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 41.5 bits (93), Expect = 4e-04 Identities = 40/161 (24%), Positives = 71/161 (44%), Gaps = 15/161 (9%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVAN-XSLRGGXLAT--------QARVWQWASWSD 226 P G+VP F DG + LTES AI+ Y+A RG L R+W + Sbjct: 86 PFGQVPVFL--DGGLKLTESRAISEYIATVHKSRGTQLLNYKSYKTMGTQRMWMAIESFE 143 Query: 227 SELLPASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLA 397 + L ++ W + P G ++ + + V ++ L L + + L +FL + T+A Sbjct: 144 FDPLTSTLTWEQSIKPMYG-LKTDYKVVNETEAKLEKVLDIYEERLKNSSFLASNSFTMA 202 Query: 398 DVIVFSTLLHAFQHVLDPSVRSSLIN---VQRWFLTVAHQP 511 D+ + Q+++D + +N V+RW + +P Sbjct: 203 DLYHLPNI----QYLMDTHTKRMFVNRPSVRRWVAEITARP 239 >At5g44000.1 68418.m05384 glutathione S-transferase C-terminal domain-containing protein contains Pfam domain PF00043: Glutathione S-transferase, C-terminal domain Length = 399 Score = 36.7 bits (81), Expect = 0.011 Identities = 22/95 (23%), Positives = 47/95 (49%), Gaps = 2/95 (2%) Frame = +2 Query: 287 NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLH--AFQHVLDPSVR 460 +++ + A ++L + L ++ HL + +L ER+TLADV +F+TL+ + ++L + Sbjct: 261 SQEAYDGAVNELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLIRFDSVYNILFKCTK 320 Query: 461 SSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTY 565 L+ + + Q+ V + + A Y Sbjct: 321 KKLVEYPNLYGYLREIYQIPGVAATCDISAIMDGY 355 >At1g02930.1 68414.m00260 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 208 Score = 34.3 bits (75), Expect = 0.061 Identities = 36/119 (30%), Positives = 51/119 (42%), Gaps = 9/119 (7%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVAN-XSLRGGXLAT----QARVWQWASWSDSELL 238 P GKVPAF D K+ ES AI Y+A+ S +G L + A + E Sbjct: 51 PFGKVPAFEDGDFKIF--ESRAITQYIAHEFSDKGNNLLSTGKDMAIIAMGIEIESHEFD 108 Query: 239 P--ASCAW--VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 403 P + W V L M +K VE ++ L L V + L +L ++ TL D+ Sbjct: 109 PVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDL 167 >At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF-1-beta, putative nearly identical to eEF-1beta [Arabidopsis thaliana] GI:398606 Length = 231 Score = 33.9 bits (74), Expect = 0.081 Identities = 21/67 (31%), Positives = 33/67 (49%) Frame = +2 Query: 326 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAH 505 + LK LD HLLTR+++ + + D+ VF+ L S +NV RWF + Sbjct: 11 SGLKKLDEHLLTRSYITGYQASKDDITVFT--------ALSKPPTSEFVNVSRWFNHIDA 62 Query: 506 QPQVSAV 526 ++S V Sbjct: 63 LLRISGV 69 >At1g02920.1 68414.m00259 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus]; supported by cDNA GI:443697. Length = 209 Score = 33.9 bits (74), Expect = 0.081 Identities = 37/120 (30%), Positives = 52/120 (43%), Gaps = 10/120 (8%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVAN-XSLRGGXLATQ-----ARVWQWASWSDSEL 235 P GKVPAF DG L ES AI Y+A+ S +G L + A + E Sbjct: 51 PFGKVPAF--EDGDFKLFESRAITQYIAHFYSDKGNQLVSLGSKDIAGIAMGIEIESHEF 108 Query: 236 LP--ASCAW--VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 403 P + W V L M +K VE ++ L L V + L +L +++ TL D+ Sbjct: 109 DPVGSKLVWEQVLKPLYGMTTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDL 168 >At2g30860.1 68415.m03761 glutathione S-transferase, putative identical to GB:Y12295 Length = 215 Score = 33.5 bits (73), Expect = 0.11 Identities = 30/120 (25%), Positives = 52/120 (43%), Gaps = 10/120 (8%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVANX------SLRGGXLATQARVWQWASWSDSEL 235 P G VPA D K+ ES A+ YVA L G + + +V QW + Sbjct: 49 PFGTVPAVVDGDYKIF--ESRAVMRYVAEKYRSQGPDLLGKTVEDRGQVEQWLDVEATTY 106 Query: 236 LPA--SCAWVFPYLGIMQF--NKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 403 P + + +M F +++ ++ ++ L L V + HL +L + ++LAD+ Sbjct: 107 HPPLLNLTLHIMFASVMGFPSDEKLIKESEEKLAGVLDVYEAHLSKSKYLAGDFVSLADL 166 >At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta identical to SP|P48006 Elongation factor 1-beta (EF-1-beta) {Arabidopsis thaliana} Length = 231 Score = 32.3 bits (70), Expect = 0.25 Identities = 21/67 (31%), Positives = 34/67 (50%) Frame = +2 Query: 326 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAH 505 A LK LD HLLTR+++ + + D+ VF+ L P+ S +N RW+ + Sbjct: 11 AGLKKLDEHLLTRSYITGYQASKDDITVFAAL------AKPPT--SQYVNASRWYNHIDA 62 Query: 506 QPQVSAV 526 ++S V Sbjct: 63 LLRISGV 69 >At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive dehalogenase-related contains similarity to tetrachloro-p-hydroquinone reductive dehalogenase GI:148689 from [Flavobacterium sp.] Length = 266 Score = 31.9 bits (69), Expect = 0.33 Identities = 20/78 (25%), Positives = 35/78 (44%) Frame = +2 Query: 299 VERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINV 478 + R+K LL L ++ L T+L ++ADV++ L L+ SS N+ Sbjct: 161 LRRSKDHLLRLLDEVETKLEGTTYLAGNEFSMADVMLIPVLARLSLLDLEEEYISSRKNL 220 Query: 479 QRWFLTVAHQPQVSAVVG 532 ++ V +P V+G Sbjct: 221 AEYWALVRRRPSYKKVIG 238 >At1g16840.2 68414.m02028 expressed protein Length = 161 Score = 31.5 bits (68), Expect = 0.43 Identities = 26/80 (32%), Positives = 34/80 (42%) Frame = +3 Query: 108 MERCF*LKAMPSLTTLPMXVSAEXLWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 287 + R F +A P LP A W+P P SGS HG+ L+ L + LT S Sbjct: 67 VHRIFVRRAQPDW--LPFVPGASY-WVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120 Query: 288 TNRMLNVQSLTYWPP*KYWT 347 TN S Y+ YW+ Sbjct: 121 TNSSHGWPSSDYFLKGSYWS 140 >At1g02950.2 68414.m00263 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 245 Score = 31.5 bits (68), Expect = 0.43 Identities = 35/122 (28%), Positives = 51/122 (41%), Gaps = 12/122 (9%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVAN-XSLRGGXL------ATQARVWQWASWSDSE 232 P G+VP F DG V L ES AI Y+A S RG L T A + W + Sbjct: 74 PFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQLLNLRSHETMATLTMWMEIEAHQ 131 Query: 233 LLP--ASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLA 397 P + W + P G ++ ++ V+ ++ L L + + L FL TL Sbjct: 132 FDPPASKLTWEQVIKPIYG-LETDQTIVKENEAILEKVLNIYEKRLEESRFLACNSFTLV 190 Query: 398 DV 403 D+ Sbjct: 191 DL 192 >At1g02950.1 68414.m00262 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 243 Score = 31.5 bits (68), Expect = 0.43 Identities = 35/122 (28%), Positives = 51/122 (41%), Gaps = 12/122 (9%) Frame = +2 Query: 74 PAGKVPAFXSADGKVLLTESNAIAYYVAN-XSLRGGXL------ATQARVWQWASWSDSE 232 P G+VP F DG V L ES AI Y+A S RG L T A + W + Sbjct: 72 PFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQLLNLRSHETMATLTMWMEIEAHQ 129 Query: 233 LLP--ASCAW---VFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLA 397 P + W + P G ++ ++ V+ ++ L L + + L FL TL Sbjct: 130 FDPPASKLTWEQVIKPIYG-LETDQTIVKENEAILEKVLNIYEKRLEESRFLACNSFTLV 188 Query: 398 DV 403 D+ Sbjct: 189 DL 190 >At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-containing protein-related contains weak hit to Pfam F00627: UBA/TS-N domain; supported by tandem duplication of ubiquitin-associated (UBA)/TS-N domain protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis thaliana] Length = 508 Score = 29.5 bits (63), Expect = 1.7 Identities = 12/40 (30%), Positives = 19/40 (47%) Frame = +3 Query: 270 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLPRES 389 L++ + N N +S + WPP K W D H + E+ Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEA 234 >At5g07270.1 68418.m00829 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 513 Score = 28.7 bits (61), Expect = 3.0 Identities = 20/50 (40%), Positives = 23/50 (46%), Gaps = 7/50 (14%) Frame = +3 Query: 303 NVQSLTYWPP*-------KYWTDIFSHAPSLLPRESHLPMSLSSVHCCML 431 NV S+T PP ++ F PS L RE LPMSL CML Sbjct: 344 NVPSVTVGPPGSIPCPLCRHGITAFKRLPSSLTREMKLPMSLGFCAPCML 393 >At3g49210.1 68416.m05378 expressed protein Length = 518 Score = 28.7 bits (61), Expect = 3.0 Identities = 19/82 (23%), Positives = 40/82 (48%) Frame = +2 Query: 206 QWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTER 385 +W +W + P S L ++ K+ ++R K+ L AAL + G + +TF V Sbjct: 361 RWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVA 420 Query: 386 ITLADVIVFSTLLHAFQHVLDP 451 + + + +T + +F +++ P Sbjct: 421 AKIINRALSNTTM-SFSNLIGP 441 >At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / long-chain acyl-CoA synthetase nearly identical to acyl CoA synthetase (MF45P) GI:1617268 from [Brassica napus] Length = 665 Score = 28.7 bits (61), Expect = 3.0 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = -2 Query: 474 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 343 F+ ++ +C K CSS LKT+ S + S ++ +C S Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199 >At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / glutamyl-tRNA synthetase, putatuve / GluRS, putative identical to gi:3435196 Length = 719 Score = 27.9 bits (59), Expect = 5.3 Identities = 15/53 (28%), Positives = 27/53 (50%) Frame = +2 Query: 341 LDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTV 499 +D +L + TFLV +++ADV ++S L Q ++ RWF ++ Sbjct: 104 VDKYLESSTFLVGHSLSIADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSI 156 >At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like transposon protein GB:AAB95292 GI:2088658 from [Arabidopsis thaliana] Length = 1148 Score = 27.9 bits (59), Expect = 5.3 Identities = 14/28 (50%), Positives = 18/28 (64%) Frame = -3 Query: 536 ASRRRPTLAAGGRRSGTNAERLSATNGR 453 +S R P+ A GRRSGT R+S T G+ Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197 >At1g47220.1 68414.m05227 cyclin, putative similar to cyclin A-like protein [Nicotiana tabacum] GI:1064931, A-type cyclin [Catharanthus roseus] GI:2190259; contains Pfam profiles PF00134: Cyclin, N-terminal domain, PF02984: Cyclin, C-terminal domain Length = 327 Score = 27.1 bits (57), Expect = 9.3 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +2 Query: 260 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 373 F Y+ + KQ+V + + D+L AL+ G T TFL Sbjct: 159 FCYITANTYTKQDVLKMEEDILLALEFELGRPTTNTFL 196 >At1g16840.4 68414.m02027 expressed protein Length = 161 Score = 27.1 bits (57), Expect = 9.3 Identities = 22/62 (35%), Positives = 28/62 (45%) Frame = +3 Query: 108 MERCF*LKAMPSLTTLPMXVSAEXLWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 287 + R F +A P LP A W+P P SGS HG+ L+ L + LT S Sbjct: 67 VHRIFVRRAQPDW--LPFVPGASY-WVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120 Query: 288 TN 293 TN Sbjct: 121 TN 122 >At1g16840.3 68414.m02026 expressed protein Length = 161 Score = 27.1 bits (57), Expect = 9.3 Identities = 22/62 (35%), Positives = 28/62 (45%) Frame = +3 Query: 108 MERCF*LKAMPSLTTLPMXVSAEXLWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 287 + R F +A P LP A W+P P SGS HG+ L+ L + LT S Sbjct: 67 VHRIFVRRAQPDW--LPFVPGASY-WVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120 Query: 288 TN 293 TN Sbjct: 121 TN 122 >At1g16840.1 68414.m02025 expressed protein Length = 161 Score = 27.1 bits (57), Expect = 9.3 Identities = 22/62 (35%), Positives = 28/62 (45%) Frame = +3 Query: 108 MERCF*LKAMPSLTTLPMXVSAEXLWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 287 + R F +A P LP A W+P P SGS HG+ L+ L + LT S Sbjct: 67 VHRIFVRRAQPDW--LPFVPGASY-WVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120 Query: 288 TN 293 TN Sbjct: 121 TN 122 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,757,871 Number of Sequences: 28952 Number of extensions: 256028 Number of successful extensions: 681 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 669 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 681 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1161268208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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