BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_D04 (751 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 341 5e-95 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 337 1e-93 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 170 2e-43 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 138 6e-34 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.0 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.6 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.7 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 341 bits (839), Expect = 5e-95 Identities = 150/217 (69%), Positives = 179/217 (82%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460 +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 640 +IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+ Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 641 VVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNL 751 VVEPYNS+LTTH TL+ +DC FMVDNE YDICRRNL Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNL 217 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 337 bits (828), Expect = 1e-93 Identities = 152/222 (68%), Positives = 182/222 (81%), Gaps = 5/222 (2%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445 TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++ Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119 Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 625 D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179 Query: 626 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNL 751 QVST+VVEPYNS+LTTH TL++SDC FMVDNE YDICRRNL Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNL 221 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 170 bits (414), Expect = 2e-43 Identities = 84/217 (38%), Positives = 120/217 (55%) Frame = +2 Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460 +VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118 Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 640 +R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178 Query: 641 VVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNL 751 VVEPYN+ L+ H +E+SD F +DNE + I L Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTL 215 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 138 bits (335), Expect = 6e-34 Identities = 77/219 (35%), Positives = 120/219 (54%), Gaps = 3/219 (1%) Frame = +2 Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457 +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 634 D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179 Query: 635 TAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNL 751 VV+PYNS+L ++D ++DN + I L Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRL 218 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.2 bits (55), Expect = 5.0 Identities = 21/74 (28%), Positives = 33/74 (44%) Frame = +3 Query: 63 NSTFKHXKKLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETI 242 +S+ + S + +S S S+ + T+ ST SSL + ++PS T Sbjct: 135 SSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTS 193 Query: 243 LSTLSSARPELAST 284 ST SSA P S+ Sbjct: 194 SSTFSSAAPTSTSS 207 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 8.7 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 90 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 212 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,006,729 Number of Sequences: 5004 Number of extensions: 62005 Number of successful extensions: 171 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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