SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P01_F_C05
         (624 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po...   231   5e-62
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc...    43   4e-05
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p...    43   4e-05
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ...    29   0.55 
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ...    29   0.72 
SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosacch...    28   1.3  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   2.2  
SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ...    27   2.2  
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom...    26   3.8  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    26   3.8  
SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase |Schizosaccha...    26   5.1  
SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|S...    25   6.7  
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|...    25   8.9  

>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 619

 Score =  231 bits (566), Expect = 5e-62
 Identities = 110/185 (59%), Positives = 142/185 (76%), Gaps = 3/185 (1%)
 Frame = +1

Query: 76  KGGLRXLANXX-HXERFGYVFAVSGPVVTAEKMSGSAMYELVRVGYNELVGEIIRLEGDM 252
           K  +R L N   H  R+G +F+VSGPVV A  M G +MYELVRVG+ ELVGE+IR+  D 
Sbjct: 9   KKAIRSLKNYDEHENRYGSIFSVSGPVVVAANMLGCSMYELVRVGHEELVGEVIRIHQDK 68

Query: 253 ATIQVYEETSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINELTQSIYIPKG 432
            TIQVYEETSG+TVGDPV RTGKPLSVELGPG+  +I+DGIQRPLK I + +QSIYIP+G
Sbjct: 69  CTIQVYEETSGLTVGDPVQRTGKPLSVELGPGLAETIYDGIQRPLKQIFDKSQSIYIPRG 128

Query: 433 INVPSLAREVDWEFNP-LNVKVGSHITGGDLYGIVHXNTLVK-HRMLVPPKAKGTVTYIX 606
           IN  SL RE  W+F P  ++++G H++GGD++G V  N+L   H++++PP+A+GTVTYI 
Sbjct: 129 INTESLNREHKWDFTPNKDLRIGDHVSGGDVFGSVFENSLFNDHKIMLPPRARGTVTYIA 188

Query: 607 PAGNY 621
            AG+Y
Sbjct: 189 EAGSY 193


>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 503

 Score = 42.7 bits (96), Expect = 4e-05
 Identities = 30/117 (25%), Positives = 56/117 (47%), Gaps = 5/117 (4%)
 Frame = +1

Query: 121 FGYVFAVSGPVVTAEKMSGSAMYELVRVGYNE---LVGEIIRLEGDMATIQVYEETSGVT 291
           +  V +++GP+V  + +      E+V +   +     G+++ + G  A +QV+E TSGV 
Sbjct: 24  YNTVNSITGPLVILDNIRRPQYNEIVNLNLPDGSVRSGQVLEVAGHKAIVQVFEGTSGVD 83

Query: 292 VGDPVLR-TGKPLSVELGPGILGSIFDGIQRPL-KDINELTQSIYIPKGINVPSLAR 456
           V    +  TG  + + +   +LG +F+G   P+ K  N L +      G  +   AR
Sbjct: 84  VRKTTIDFTGHSMRIPVSEDMLGRVFNGSGLPIDKGPNLLAEDYLDINGSPINPYAR 140


>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 525

 Score = 42.7 bits (96), Expect = 4e-05
 Identities = 23/73 (31%), Positives = 37/73 (50%)
 Frame = +1

Query: 241 EGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINELTQSIY 420
           E  + TI + + T G+  G  V+ TG P+S+ +GPG LG I + I  P+ +   +    Y
Sbjct: 102 ENTVRTIAM-DGTEGLVRGTAVIDTGSPISIPVGPGTLGRIMNVIGEPVDERGPIKAVKY 160

Query: 421 IPKGINVPSLARE 459
            P   + PS   +
Sbjct: 161 SPIHADAPSFEEQ 173


>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
           membrane transporter Hmt1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 830

 Score = 29.1 bits (62), Expect = 0.55
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -3

Query: 307 VLDHLQLHLKFLRIPGWWPC 248
           V+D   L +  LR+P WWPC
Sbjct: 67  VVDISNLVIYALRVPNWWPC 86


>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 28.7 bits (61), Expect = 0.72
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -2

Query: 374 IPSKMEPKIPGPSSTDKGFPVRSTGSPTVTPE 279
           IPS + P    P  T  GFP R    PT+ P+
Sbjct: 368 IPSTLPPADGVPKPTPDGFPRRFERDPTIPPD 399



 Score = 25.0 bits (52), Expect = 8.9
 Identities = 12/44 (27%), Positives = 24/44 (54%)
 Frame = +2

Query: 116 SGSDMSSPYLDPSSLPKKCPDLLCTSWSVSVTMNSSVRLSVLKV 247
           SG+ +S+P   PS++P+  P       + SV    S +++++ V
Sbjct: 251 SGNPLSNPLFQPSNVPQSIPSGPMGMKTGSVNDTQSQQITLMNV 294


>SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1294

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 23/116 (19%), Positives = 48/116 (41%)
 Frame = +1

Query: 115 ERFGYVFAVSGPVVTAEKMSGSAMYELVRVGYNELVGEIIRLEGDMATIQVYEETSGVTV 294
           E    VF++   +++  ++S + +Y +V V      G +       +  Q+ E+      
Sbjct: 203 EDIALVFSLLNCIISYGRISSAVLYSIVEVVCRAKFGLVA---SSQSAQQIVEKLLKTAT 259

Query: 295 GDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINELTQSIYIPKGINVPSLAREV 462
               L + K +  E   G   +I  GIQ       ++ +S+Y  +G+ +  L R +
Sbjct: 260 KYEALNSLKRIMEETDEGSYIAIMGGIQIVSALFTDIPRSVYCTRGMLLGFLQRSL 315


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 13/21 (61%), Positives = 14/21 (66%)
 Frame = -2

Query: 182 ADPDIFSAVTTGPDTAKTYPN 120
           AD  +F AV   PDTAK YPN
Sbjct: 33  ADAVVFKAVGVAPDTAK-YPN 52


>SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 761

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 17/51 (33%), Positives = 24/51 (47%)
 Frame = -1

Query: 240 KTDNLTDEFIVTDTDQLVHSRSGHFFGSDDGSRYGEDISEPLXMXXIGERP 88
           +T +L  E    DTD+ VHS+S    GS+  S    D  E   +  +G  P
Sbjct: 180 RTPSLVPEHYFDDTDKSVHSKSSS--GSNSLSEASNDDLESSIIQIVGGLP 228


>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1016

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 12/33 (36%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
 Frame = +1

Query: 241 EGDMATIQVYEETS-GVTVGDPVLRTGKPLSVE 336
           E D A++ +   T+  +T  DPV  + KPL++E
Sbjct: 572 ENDKASVSITTTTTPSITPADPVPTSPKPLAIE 604


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 15/44 (34%), Positives = 18/44 (40%)
 Frame = -2

Query: 593 TVPLALGGTNILCLTRVXSCTIPYKSPPVMWDPTLTFNGLNSQS 462
           TVP     T +       S ++PY S PV   P  T N   S S
Sbjct: 412 TVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455


>SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 547

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 19/73 (26%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
 Frame = +1

Query: 340 GPGILGSIFDGIQRPLKDINELTQSIYIPKGINVPSLAREVDWEFNPLNVKVGSHITGGD 519
           G  ++ S+   + R LKDI   T+++       + S A ++D +  P+  K  SH+    
Sbjct: 264 GQELIPSVIGPMARSLKDIEFFTETV-------IASEAWKIDSKLLPIPWKNQSHLKSKK 316

Query: 520 L-YGIVHXNTLVK 555
           L +G++  + +VK
Sbjct: 317 LIFGVLKTDGIVK 329


>SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter
           Atm1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 693

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +1

Query: 277 TSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINEL 405
           TS +TVGD V+       + +    LGS++  +++   D+ +L
Sbjct: 375 TSNLTVGDLVMINQLVFQLSIPLNFLGSVYREMRQAFTDMEQL 417


>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
           Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 624

 Score = 25.0 bits (52), Expect = 8.9
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = -2

Query: 494 TLTFNGLNSQSTSLAREGTLI 432
           T  FNGL +  TSLA + TL+
Sbjct: 24  TFGFNGLGTSRTSLAPQRTLV 44


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,615,573
Number of Sequences: 5004
Number of extensions: 53601
Number of successful extensions: 182
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -