BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fcaL-P01_F_B20 (723 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18.09c |||conserved eukaryotic protein|Schizosaccharomyces p... 69 7e-13 SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual 36 0.006 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 2.1 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 27 2.7 SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S... 26 4.7 SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.3 SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo... 25 8.3 >SPCC18.09c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 232 Score = 68.9 bits (161), Expect = 7e-13 Identities = 50/170 (29%), Positives = 85/170 (50%), Gaps = 21/170 (12%) Frame = +2 Query: 179 IASMKDPNSIIKNTEKVVVIKDKYPKAKVHYLVLPHE-EINSIYKLN--KSHISLLEEF- 346 I S + ++I + VV+++D +PK+K+H L++ + + ++ L H SL+E+ Sbjct: 42 IESPESYKNVIYYDDDVVLVRDMFPKSKMHLLLMTRDPHLTHVHPLEIMMKHRSLVEKLV 101 Query: 347 --------GNIFKELKE------ENES---ELRAGFHAIPSMQRMHMHVISTDMISTSLK 475 G IF E + NE+ ++ GFHA PSM +H+H+++ D +S SLK Sbjct: 102 SYVQGDLSGLIFDEARNCLSQQLTNEALCNYIKVGFHAGPSMNNLHLHIMTLDHVSPSLK 161 Query: 476 TKIHWNSFCTKFFIPYDELLQELKDIGNIRKIPSELHTSLMKTPLQCNQC 625 H+ SF + FF+ D L G + TSL + L+C +C Sbjct: 162 NSAHYISFTSPFFVKIDTPTSNLPTRGTL--------TSLFQEDLKCWRC 203 >SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 35.9 bits (79), Expect = 0.006 Identities = 20/76 (26%), Positives = 41/76 (53%), Gaps = 5/76 (6%) Frame = +2 Query: 242 DKYPKAKVHYLVLPHE-EINSIYKLNKSHISLLEEFGN-IFKELKEE---NESELRAGFH 406 D+ + ++ + + H +I SI L HI LLE N + E+ ++ ++++L+ H Sbjct: 176 DRQTMSALNLMAIVHATDIASIRDLKYKHIPLLENIRNKVLTEVPKQFSVDKNQLKMFVH 235 Query: 407 AIPSMQRMHMHVISTD 454 +PS +H+H++ D Sbjct: 236 YLPSYYHLHVHILHVD 251 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 27.5 bits (58), Expect = 2.1 Identities = 11/42 (26%), Positives = 24/42 (57%) Frame = +2 Query: 266 HYLVLPHEEINSIYKLNKSHISLLEEFGNIFKELKEENESEL 391 ++++ PH + + K+ + H +L +F +F+ L ENE + Sbjct: 408 YFMIFPHSDQSLQNKVFELHSTLAIKFDELFRLLNLENEESV 449 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 27.1 bits (57), Expect = 2.7 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = +2 Query: 287 EEINSIYKLNKSHISLLEEFGNIFKEL 367 EE+ S++ ++KS S +FGN+F L Sbjct: 1321 EEVCSVFNISKSVCSKYVQFGNVFNLL 1347 >SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|Schizosaccharomyces pombe|chr 3|||Manual Length = 957 Score = 26.2 bits (55), Expect = 4.7 Identities = 22/95 (23%), Positives = 41/95 (43%), Gaps = 2/95 (2%) Frame = +2 Query: 266 HYLVLPH--EEINSIYKLNKSHISLLEEFGNIFKELKEENESELRAGFHAIPSMQRMHMH 439 H+L+ H EI +Y L S ++ ++ S ++ H I + + Sbjct: 317 HFLISDHIFSEIQKVYFLPSSQNDSFDDVVACLRQKSTPYLSFIKKARHLIQVSRDKYKL 376 Query: 440 VISTDMISTSLKTKIHWNSFCTKFFIPYDELLQEL 544 IST+ I + +++ W F K + Y L+QE+ Sbjct: 377 PISTEEIKPVVYSQVTWTEF-EKKLLRY--LVQEM 408 >SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 657 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = +2 Query: 284 HEEINSIYKLNKSHISLLEEFGNIFKELKEENESEL 391 H+ +N++ + K + LEE G+ KEL EE++ + Sbjct: 545 HKALNTLDEETKHQV--LEEIGDYVKELDEESKKAI 578 >SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +2 Query: 470 LKTKIHWNSFCTKFFIPYDELLQELKDIGNIRKIP 574 L+T IH +FC FF + KD+ RK P Sbjct: 3 LETLIHGVTFCVPFFSKSARIHTLSKDVFQQRKFP 37 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,427,807 Number of Sequences: 5004 Number of extensions: 44404 Number of successful extensions: 142 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 141 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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