BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbpv0805
(589 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 34 0.018
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 29 0.67
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc... 26 4.7
SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 25 6.2
SPBC211.06 |gfh1||gamma tubulin complex subunit Gfh1|Schizosacch... 25 8.2
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |... 25 8.2
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 33.9 bits (74), Expect = 0.018
Identities = 17/72 (23%), Positives = 40/72 (55%), Gaps = 2/72 (2%)
Frame = +1
Query: 88 RLLXHKIRNLEKRKSKLT-SYRD-LQKAGKELNSDQKVAVAKYDEVAQTLEFARDLSNKL 261
R L H++ +L+ + L + D ++K E+++ + + K DE+ ++++ +L K+
Sbjct: 108 RRLSHEVNDLQTDRENLKHQFEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKI 167
Query: 262 LI*QSLQSVRQK 297
+S QS+ Q+
Sbjct: 168 NSLESAQSIEQE 179
>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1052
Score = 28.7 bits (61), Expect = 0.67
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 2/76 (2%)
Frame = -1
Query: 322 TQASFLACFFASRSEEIAISVTCLRDLLQIQGFGQLHHI*QLQLFDQNLALFQLSAGHDM 143
T +F A F + S +I++ +DL IQ L + L L + L L L H
Sbjct: 629 TSVNF-APIFTTPSRVRSITLLLPKDLANIQPSDLLFYAVLLVLINGKLVLLSLKKQHSK 687
Query: 142 MLVYSC--VFPNYEFY 101
L+Y C + + EFY
Sbjct: 688 ELLYQCSMLAGDVEFY 703
>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 807
Score = 25.8 bits (54), Expect = 4.7
Identities = 11/41 (26%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Frame = -1
Query: 565 RGVLQLLLFEGETRLXFXINSCLGV--TSGYKSXKIFKSSS 449
RG+ +LLLFEG+ + + +N + + G+++ ++ + S
Sbjct: 568 RGLKELLLFEGDVKNTYGLNFTINLKAVEGFRTVELKEGGS 608
>SPCC1682.16 |rpt4||19S proteasome regulatory subunit
Rpt4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 388
Score = 25.4 bits (53), Expect = 6.2
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 115 LEKRKSKLTSYRDLQKAGKELNSDQKVAVAKYDEVAQTLE 234
LEK KS L +R+ K+L + V KYD+ ++
Sbjct: 8 LEKYKSYLLQHREWDSKLKDLRFGNRDLVKKYDKTEDDIK 47
>SPBC211.06 |gfh1||gamma tubulin complex subunit
Gfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 577
Score = 25.0 bits (52), Expect = 8.2
Identities = 14/54 (25%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = -3
Query: 233 SRVWATSSYLATATF*S-EFSSFPAFCRSRYDVSLLLRFSKLRILWXNNRHNLS 75
+++W T +L++ + + E P++C+ R ++ L R KLR+ + H L+
Sbjct: 449 TQLWVTLWFLSSLQYYAYECVIKPSYCKLRESLTELYRTQKLRMQDCSQLHALT 502
>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1032
Score = 25.0 bits (52), Expect = 8.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +1
Query: 295 KNKLKRKPGFVMQQRPTK*KKFYLF 369
KN+ + K F +Q+RP K +++LF
Sbjct: 1008 KNQQRNKEKFRIQKRPNKKYRYHLF 1032
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,840
Number of Sequences: 5004
Number of extensions: 37023
Number of successful extensions: 115
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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