Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbpv0601
(811 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 93 1e-19
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 60 2e-09
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 57 1e-08
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 57 1e-08
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 38 0.006
At2g27035.1 68415.m03248 plastocyanin-like domain-containing pro... 30 1.6
At1g66110.1 68414.m07503 hypothetical protein contains Pfam prof... 30 2.1
At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containi... 29 2.8
At4g22190.1 68417.m03208 expressed protein 29 3.7
At1g75800.1 68414.m08805 pathogenesis-related thaumatin family p... 29 3.7
At4g14060.1 68417.m02171 major latex protein-related / MLP-relat... 28 8.4
At2g01520.1 68415.m00076 major latex protein-related / MLP-relat... 28 8.4
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 93.5 bits (222), Expect = 1e-19
Identities = 60/159 (37%), Positives = 86/159 (54%), Gaps = 7/159 (4%)
Frame = +1
Query: 256 KLMNRLWGENFFNPQTKKWSKQKDDDN--KRSFCMYVLDPIYKVFDAIMKFKKEEIDDLL 429
K+M RLWGENFF+P T+KWS + KR F + +PI ++ M +K+++ +L
Sbjct: 239 KMMERLWGENFFDPATRKWSGKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPML 298
Query: 430 KKIGVTIKHEDSDKDGKALLKVVMR-----SCCLLVKLCFR*LPFIYHHL*WPRNIVWRC 594
K+GV++K+++ + GK L+K VM+ S LL + F LP + R V
Sbjct: 299 AKLGVSMKNDEKELMGKPLMKRVMQTWLPASTALLEMMIFH-LPSPHTA---QRYRVENL 354
Query: 595 YTXDPTMMXLPLVSRAVIPEAPLMMYVSKMVPTSDKGRF 711
Y P R P PLM+YVSKM+P SDKGRF
Sbjct: 355 Y-EGPLDDQYANAIRNCDPNGPLMLYVSKMIPASDKGRF 392
Score = 89.0 bits (211), Expect = 3e-18
Identities = 45/89 (50%), Positives = 60/89 (67%), Gaps = 2/89 (2%)
Frame = +2
Query: 59 YQTFQRIVENVNVIIATYNDDGGPMGDVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADK 238
YQTF R++EN NVI+ATY D +GDV+V P KG+V F +GLHGWAFTL F++MYA K
Sbjct: 175 YQTFSRVIENANVIMATYEDP--LLGDVQVYPEKGTVAFSAGLHGWAFTLTNFAKMYASK 232
Query: 239 F-KIDLASL*TGYGEKTFSTLKRR-SGQN 319
F ++ + +GE F R+ SG+N
Sbjct: 233 FGVVESKMMERLWGENFFDPATRKWSGKN 261
Score = 60.9 bits (141), Expect = 1e-09
Identities = 28/50 (56%), Positives = 34/50 (68%)
Frame = +3
Query: 510 LPAGEALLQMIAIHLPSPVVAQKYRMEMLYXGPHDDXAAIGIKSCDS*SP 659
LPA ALL+M+ HLPSP AQ+YR+E LY GP DD A I++CD P
Sbjct: 326 LPASTALLEMMIFHLPSPHTAQRYRVENLYEGPLDDQYANAIRNCDPNGP 375
Score = 52.8 bits (121), Expect = 3e-07
Identities = 22/33 (66%), Positives = 28/33 (84%)
Frame = +2
Query: 710 FYAFGRVFSGKVVTGPKSRIMGPNFTPGKERGL 808
F+AFGRVF+GKV TG K RIMGPN+ PG+++ L
Sbjct: 392 FFAFGRVFAGKVSTGMKVRIMGPNYIPGEKKDL 424
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 59.7 bits (138), Expect = 2e-09
Identities = 38/156 (24%), Positives = 75/156 (48%), Gaps = 4/156 (2%)
Frame = +1
Query: 256 KLMNRLWGENFFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLK 432
K +RLWG+ +++P T+ ++ +R+F ++L+P+YK++ ++ K+ ++ L
Sbjct: 338 KFASRLWGDVYYHPDTRVFNTSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLA 397
Query: 433 KIGVTIKHEDSDKDGKALLKVVMRSCCLLVKLCFR*LPFIYHHL*WPRNIVWRCYTXDPT 612
++GVT+ + + + LL++ +C + + H+ PR R T
Sbjct: 398 ELGVTLSNSAYKLNVRPLLRL---ACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYT 454
Query: 613 -MMXLPLVSRAV--IPEAPLMMYVSKMVPTSDKGRF 711
P+ V P PLM+ V+K+ P SD F
Sbjct: 455 GTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVF 490
Score = 41.9 bits (94), Expect = 5e-04
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = +2
Query: 146 VDPSKGSVGFGSGLHGWAFTLKQFSEMYA 232
+DP+ G+V F SG GW+FTL+ F+ MYA
Sbjct: 299 IDPAAGNVCFASGTAGWSFTLQSFARMYA 327
Score = 35.9 bits (79), Expect = 0.032
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +2
Query: 707 VFYAFGRVFSGKVVTGPKSRIMGPNFTPGKERGL 808
VF FGRV+SG++ TG R++G ++P E +
Sbjct: 489 VFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDM 522
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 57.2 bits (132), Expect = 1e-08
Identities = 37/156 (23%), Positives = 74/156 (47%), Gaps = 4/156 (2%)
Frame = +1
Query: 256 KLMNRLWGENFFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLK 432
K +RLWG+ +++ T+ + + +R+F ++L+P+YK++ ++ K+ ++ L
Sbjct: 352 KFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLA 411
Query: 433 KIGVTIKHEDSDKDGKALLKVVMRSCCLLVKLCFR*LPFIYHHL*WPRNIVWRCYTXDPT 612
++GVT+ + + + LL++ +C + + H+ PR R T
Sbjct: 412 ELGVTLSNSAYKLNVRPLLRL---ACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYT 468
Query: 613 -MMXLPLVSRAV--IPEAPLMMYVSKMVPTSDKGRF 711
P+ V P PLM+ V+K+ P SD F
Sbjct: 469 GTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVF 504
Score = 44.4 bits (100), Expect = 9e-05
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 59 YQTFQRIVENVNVIIATYNDDGGPMGDVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYA 232
Y + +E +N I+ + G + +DP+ G+V F SG GW+FTL+ F++MYA
Sbjct: 286 YYKLRHTIEVINNHISAASTTAGDLP--LIDPAAGNVCFASGTAGWSFTLQSFAKMYA 341
Score = 35.9 bits (79), Expect = 0.032
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +2
Query: 707 VFYAFGRVFSGKVVTGPKSRIMGPNFTPGKERGL 808
VF FGRV+SG++ TG R++G ++P E +
Sbjct: 503 VFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDM 536
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 57.2 bits (132), Expect = 1e-08
Identities = 37/156 (23%), Positives = 74/156 (47%), Gaps = 4/156 (2%)
Frame = +1
Query: 256 KLMNRLWGENFFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLK 432
K +RLWG+ +++ T+ + + +R+F ++L+P+YK++ ++ K+ ++ L
Sbjct: 352 KFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLA 411
Query: 433 KIGVTIKHEDSDKDGKALLKVVMRSCCLLVKLCFR*LPFIYHHL*WPRNIVWRCYTXDPT 612
++GVT+ + + + LL++ +C + + H+ PR R T
Sbjct: 412 ELGVTLSNSAYKLNVRPLLRL---ACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYT 468
Query: 613 -MMXLPLVSRAV--IPEAPLMMYVSKMVPTSDKGRF 711
P+ V P PLM+ V+K+ P SD F
Sbjct: 469 GTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVF 504
Score = 44.4 bits (100), Expect = 9e-05
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +2
Query: 59 YQTFQRIVENVNVIIATYNDDGGPMGDVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYA 232
Y + +E +N I+ + G + +DP+ G+V F SG GW+FTL+ F++MYA
Sbjct: 286 YYKLRHTIEVINNHISAASTTAGDLP--LIDPAAGNVCFASGTAGWSFTLQSFAKMYA 341
Score = 35.9 bits (79), Expect = 0.032
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +2
Query: 707 VFYAFGRVFSGKVVTGPKSRIMGPNFTPGKERGL 808
VF FGRV+SG++ TG R++G ++P E +
Sbjct: 503 VFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDM 536
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 38.3 bits (85), Expect = 0.006
Identities = 25/88 (28%), Positives = 46/88 (52%), Gaps = 7/88 (7%)
Frame = +1
Query: 259 LMNRLWGENFFNPQTK----KWSKQKDDDNKRSFCMYVLDPIYKVFDAIMK--FKKEEID 420
L LWG ++ P+TK K + K F +VL+P+++V++A + K ++
Sbjct: 242 LQKSLWGPRYYIPKTKMIVGKKNLSAGSKAKPMFVQFVLEPLWQVYEAALDPGGDKAVLE 301
Query: 421 DLLKKIGVTI-KHEDSDKDGKALLKVVM 501
++K ++I E +KD K +L+ VM
Sbjct: 302 KVIKSFNLSIPPRELQNKDPKNVLQSVM 329
Score = 35.1 bits (77), Expect = 0.056
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +2
Query: 137 DVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYADKFKIDLASL 262
+V P KG+V F L GW F + +F+ YA K +L
Sbjct: 201 EVTFQPQKGNVVFVCALDGWGFGIAEFANFYASKLGASATAL 242
Score = 33.9 bits (74), Expect = 0.13
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +3
Query: 510 LPAGEALLQMIAIHLPSPVVAQKYRMEML 596
LP +A+L M HLP P+ AQ YR+ L
Sbjct: 333 LPLSDAVLSMAVKHLPDPIAAQAYRIPRL 361
>At2g27035.1 68415.m03248 plastocyanin-like domain-containing
protein low similarity to SP:P80728 Mavicyanin
{Cucurbita pepo}; contains Pfam profile PF02298:
Plastocyanin-like domain
Length = 163
Score = 30.3 bits (65), Expect = 1.6
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = -2
Query: 183 PDPKPTEPLLGSTRTSPMGPPSSLYVAIMTLTFSTI 76
P P P+ P ++ T+P+ PP+++ AI+ F +
Sbjct: 126 PQPPPSAPTNFTSTTTPLIPPNAITAAILIFAFKAL 161
>At1g66110.1 68414.m07503 hypothetical protein contains Pfam profile
PF04510: Family of unknown function (DUF577)
Length = 338
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 2/59 (3%)
Frame = +1
Query: 340 RSFCMYVLDPIYKVFDAIM-KFKKEEIDDLLKKIGV-TIKHEDSDKDGKALLKVVMRSC 510
RSF +Y+LD + D K E +D + ++ + E S +D K L ++V R C
Sbjct: 95 RSFSLYLLDSLLTDIDDFRGGLKTESLDVIKNRVSTCLLSQETSYEDFKLLSRIVSRVC 153
>At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 970
Score = 29.5 bits (63), Expect = 2.8
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +2
Query: 167 VGFGSGLHGWAFTLKQFSEMYADKFKIDLAS 259
+G G G+HGWA L+ E+ + +D+ S
Sbjct: 308 IGLGKGVHGWAVKLRLDKELVLNNALMDMYS 338
>At4g22190.1 68417.m03208 expressed protein
Length = 387
Score = 29.1 bits (62), Expect = 3.7
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -1
Query: 781 VWSHDATFWSGNNLARENASKGVENDLCRRSAPSCSRTSSVGLQESQ 641
+W + +GNN+ R S G SAP CSR++S G +S+
Sbjct: 216 IWPFSRSRSAGNNVTRPRMSFGAPTTRKVSSAP-CSRSNSTGESKSR 261
>At1g75800.1 68414.m08805 pathogenesis-related thaumatin family
protein similar to receptor serine/threonine kinase PR5K
[Arabidopsis thaliana] GI:1235680; contains Pfam
profile: PF00314 Thaumatin family
Length = 330
Score = 29.1 bits (62), Expect = 3.7
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -2
Query: 183 PDPKPTEPLLGSTRTSPMGPPSSLYVAIMT 94
PDPKPT P G++ T+P G S+ + + T
Sbjct: 262 PDPKPTTP-TGTSSTTPAGDSSTTWSPVDT 290
>At4g14060.1 68417.m02171 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:294060] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 8.4
Identities = 20/73 (27%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
Frame = +1
Query: 295 PQTKKWSKQKDDDNKRSFCM----YVLDPIYKVFDAIMKFKKEEIDDLLKKIGVT-IKHE 459
P+ K ++ DD+N + +V++ + KV+D I++F ++ DD++ KI +T K
Sbjct: 66 PEMFKERREIDDENMAVTFVGLEGHVMEQL-KVYDTILQFIQKSPDDIVCKITMTWEKRA 124
Query: 460 DSDKDGKALLKVV 498
D + +K+V
Sbjct: 125 DDSPEPSNYMKLV 137
>At2g01520.1 68415.m00076 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:169000] contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 8.4
Identities = 17/62 (27%), Positives = 35/62 (56%), Gaps = 4/62 (6%)
Frame = +1
Query: 295 PQTKKWSKQKDDDNK----RSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHED 462
P+ K ++ DD+N R +V++ + KV+D I +F ++ DD++ KI + + ++
Sbjct: 66 PEVFKERREIDDENMAVTFRGLEGHVMEQL-KVYDVIFQFIQKSPDDIICKITMIWEKQN 124
Query: 463 SD 468
D
Sbjct: 125 DD 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,402,464
Number of Sequences: 28952
Number of extensions: 408480
Number of successful extensions: 1272
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1265
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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