BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0490 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 118 5e-28 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 68 1e-12 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 62 4e-11 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 60 3e-10 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 37 0.003 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 37 0.003 SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 3.6 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.6 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 4.8 SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 25 6.3 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 6.3 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 25 8.3 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 118 bits (285), Expect = 5e-28 Identities = 54/60 (90%), Positives = 56/60 (93%) Frame = +3 Query: 78 DEEXAALVXDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGXEAQSK 257 +EE AALV DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVG EAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Score = 117 bits (282), Expect = 1e-27 Identities = 55/69 (79%), Positives = 56/69 (81%) Frame = +2 Query: 260 GILTLKYPIEHGIVTNXDDMEKIXHHTFYNELRVAPEEXPVLLTXXPLXPKXNREKMTQI 439 GILTLKYPIEHGIV N DDMEKI HHTFYNELRVAPEE P LLT PL PK NREKMTQI Sbjct: 63 GILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQI 122 Query: 440 MFXTFXXXA 466 +F TF A Sbjct: 123 IFETFNAPA 131 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 67.7 bits (158), Expect = 1e-12 Identities = 30/50 (60%), Positives = 37/50 (74%) Frame = +3 Query: 105 DNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGXEAQS 254 DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ Sbjct: 15 DNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQN 64 Score = 55.6 bits (128), Expect = 5e-09 Identities = 27/70 (38%), Positives = 41/70 (58%), Gaps = 1/70 (1%) Frame = +2 Query: 260 GILTLKYPIEHGIVTNXDDMEKIXHHTFYNE-LRVAPEEXPVLLTXXPLXPKXNREKMTQ 436 G+L ++ PIE GI+ N DME+I + + ++ L PEE P+LLT PL N+EK+ + Sbjct: 67 GLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAE 126 Query: 437 IMFXTFXXXA 466 + T A Sbjct: 127 YFYETLNVPA 136 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 62.5 bits (145), Expect = 4e-11 Identities = 24/64 (37%), Positives = 43/64 (67%) Frame = +2 Query: 263 ILTLKYPIEHGIVTNXDDMEKIXHHTFYNELRVAPEEXPVLLTXXPLXPKXNREKMTQIM 442 +L +KYP+E+GI+ + ++M ++ +TF+ +L++ P +LLT P+ P NREKM + M Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123 Query: 443 FXTF 454 F + Sbjct: 124 FERY 127 Score = 51.2 bits (117), Expect = 1e-07 Identities = 28/59 (47%), Positives = 36/59 (61%), Gaps = 2/59 (3%) Frame = +3 Query: 84 EXAALVXDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGXEAQS 254 E A +V DNG+G K G+A D+ PR FPSIVGRP R + + KD VG EA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 59.7 bits (138), Expect = 3e-10 Identities = 27/62 (43%), Positives = 35/62 (56%) Frame = +2 Query: 269 TLKYPIEHGIVTNXDDMEKIXHHTFYNELRVAPEEXPVLLTXXPLXPKXNREKMTQIMFX 448 +L YPI HG + N D ME+ + + LR PE+ LLT PL P NRE +IMF Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 449 TF 454 +F Sbjct: 149 SF 150 Score = 41.5 bits (93), Expect = 9e-05 Identities = 20/49 (40%), Positives = 30/49 (61%), Gaps = 2/49 (4%) Frame = +3 Query: 96 LVXDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYV 236 ++ DNG+G K G+AG+DAP VFP+++ R G G + K SY+ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYM 55 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 36.7 bits (81), Expect = 0.003 Identities = 17/58 (29%), Positives = 31/58 (53%) Frame = +2 Query: 281 PIEHGIVTNXDDMEKIXHHTFYNELRVAPEEXPVLLTXXPLXPKXNREKMTQIMFXTF 454 P E +++N D ME++ +TF +L + E P+ +T P R MT+++F + Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELY 142 Score = 34.3 bits (75), Expect = 0.014 Identities = 15/32 (46%), Positives = 21/32 (65%) Frame = +3 Query: 96 LVXDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 191 LV DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 36.7 bits (81), Expect = 0.003 Identities = 15/34 (44%), Positives = 22/34 (64%) Frame = +3 Query: 81 EEXAALVXDNGSGMCKAGFAGDDAPRAVFPSIVG 182 +E +A+V D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 425 Score = 26.2 bits (55), Expect = 3.6 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -3 Query: 135 NRPCTYRSHCLLPTRQXP 82 NRP YRSH + TR+ P Sbjct: 323 NRPSNYRSHSSMGTRRSP 340 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.6 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -2 Query: 217 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 125 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 25.8 bits (54), Expect = 4.8 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 141 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGXEAQS 254 G+ PRA F ++ P H G+++ M KD G E S Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMS 537 >SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = -1 Query: 239 TYVRVLLSHTDHHALMAGPSDDRGEYGARSIISCETGLAHTG 114 TY +LL D + + G +DR + + + + C L H+G Sbjct: 226 TYPSLLLEEWDRYLMQNGSENDRPDSYSSTQLYCVLCLDHSG 267 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 25.4 bits (53), Expect = 6.3 Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 1/55 (1%) Frame = -2 Query: 238 PTYESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSXTNAAXSSS 77 PT S P+ PT+ P P N S P PL+ TN S+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 25.0 bits (52), Expect = 8.3 Identities = 10/15 (66%), Positives = 14/15 (93%) Frame = -3 Query: 222 SVPYRPSRPDGGAFR 178 SVPY PS+P+GGA++ Sbjct: 87 SVPY-PSKPNGGAYQ 100 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,097,840 Number of Sequences: 5004 Number of extensions: 36298 Number of successful extensions: 86 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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