BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0428 (546 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 131 6e-32 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 85 6e-18 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 70 2e-13 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 66 2e-12 SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 25 7.3 SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces... 25 9.6 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 131 bits (317), Expect = 6e-32 Identities = 56/87 (64%), Positives = 70/87 (80%) Frame = +2 Query: 8 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYV 187 MREIVH+QAGQCGNQ+G+ FW I+DEHG+D+ G+Y G S+ Q +R+NVY+NEA+ GKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 188 PRAVLVDLXPGTMXAVTSXPYGSCSGP 268 PRAVLVDL PGTM AV S +G+ P Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRP 87 Score = 46.8 bits (106), Expect = 2e-06 Identities = 19/38 (50%), Positives = 23/38 (60%) Frame = +1 Query: 256 LFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369 LFRP N ++G SGAGN WA HY GA + V+R Sbjct: 84 LFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121 Score = 36.3 bits (80), Expect = 0.003 Identities = 16/24 (66%), Positives = 16/24 (66%) Frame = +3 Query: 417 HSLGGGTGXXXGTLXLSNXXXEYP 488 HSLGGGTG GTL LS EYP Sbjct: 137 HSLGGGTGSGMGTLLLSKIREEYP 160 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 85.0 bits (201), Expect = 6e-18 Identities = 36/86 (41%), Positives = 56/86 (65%) Frame = +2 Query: 11 REIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYVP 190 REI+ +QAGQCGNQIGS+FW+ + EHG+ G + + +DR +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 191 RAVLVDLXPGTMXAVTSXPYGSCSGP 268 RA+L+DL P + + S YGS P Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNP 88 Score = 27.9 bits (59), Expect = 1.0 Identities = 11/35 (31%), Positives = 15/35 (42%) Frame = +3 Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521 HS+ GGTG G+ L YP + +P Sbjct: 139 HSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173 Score = 24.6 bits (51), Expect = 9.6 Identities = 11/25 (44%), Positives = 13/25 (52%), Gaps = 2/25 (8%) Frame = +1 Query: 244 TLRKLFRPXNFVF--GXSGAGNNWA 312 T L+ P N + GAGNNWA Sbjct: 81 TYGSLYNPENILITKNGGGAGNNWA 105 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 70.1 bits (164), Expect = 2e-13 Identities = 32/83 (38%), Positives = 46/83 (55%), Gaps = 2/83 (2%) Frame = +2 Query: 8 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLD--RINVYYNEASSGK 181 MREI+ + GQ G QIG+ WE+ EHG+ G E+ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 182 YVPRAVLVDLXPGTMXAVTSXPY 250 YVPR++ VDL P + V + PY Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPY 83 Score = 34.7 bits (76), Expect = 0.009 Identities = 16/40 (40%), Positives = 18/40 (45%) Frame = +1 Query: 250 RKLFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369 R LF P + G A NN+A HY G V T IR Sbjct: 84 RDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIR 123 Score = 30.3 bits (65), Expect = 0.19 Identities = 14/35 (40%), Positives = 15/35 (42%) Frame = +3 Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521 HS GGGTG G L L EY + YP Sbjct: 139 HSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 66.5 bits (155), Expect = 2e-12 Identities = 34/88 (38%), Positives = 48/88 (54%), Gaps = 7/88 (7%) Frame = +2 Query: 8 MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTG-------VYKGESDLQLDRINVYYNE 166 MRE++ V GQ G QIG+ WE+ EHG+ G V+K S L D +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLN-DGFGTFFSE 59 Query: 167 ASSGKYVPRAVLVDLXPGTMXAVTSXPY 250 GK+VPR++ VDL P + V + PY Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPY 87 Score = 32.3 bits (70), Expect = 0.048 Identities = 15/40 (37%), Positives = 18/40 (45%) Frame = +1 Query: 250 RKLFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369 + LF P V G A NN+A HY G + S IR Sbjct: 88 KDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127 Score = 29.9 bits (64), Expect = 0.26 Identities = 14/35 (40%), Positives = 15/35 (42%) Frame = +3 Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521 HS GGGTG G L L EY + YP Sbjct: 143 HSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177 >SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Manual Length = 918 Score = 25.0 bits (52), Expect = 7.3 Identities = 19/68 (27%), Positives = 35/68 (51%), Gaps = 7/68 (10%) Frame = +2 Query: 74 IISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYV--PRAVL----VDLXPG-TMXA 232 I+ +HG+D G + G+S +++ + Y++ + Y+ P VL VDL P ++ Sbjct: 281 IVMGDHGMDNKGNHGGDSFDEINSVLWMYSKKPTFGYLKQPGKVLSANQVDLVPTLSLLL 340 Query: 233 VTSXPYGS 256 PYG+ Sbjct: 341 GNPIPYGN 348 >SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 24.6 bits (51), Expect = 9.6 Identities = 8/22 (36%), Positives = 15/22 (68%) Frame = +1 Query: 19 SPRASRPMRESDRIEVLGDNLR 84 SP++ P+ +S + LG+NL+ Sbjct: 242 SPKSENPLNQSSFLSFLGENLK 263 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,531,922 Number of Sequences: 5004 Number of extensions: 24146 Number of successful extensions: 53 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 43 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 51 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 225926624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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