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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbpv0428
         (546 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   131   6e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    85   6e-18
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    70   2e-13
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    66   2e-12
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma...    25   7.3  
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces...    25   9.6  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  131 bits (317), Expect = 6e-32
 Identities = 56/87 (64%), Positives = 70/87 (80%)
 Frame = +2

Query: 8   MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYV 187
           MREIVH+QAGQCGNQ+G+ FW  I+DEHG+D+ G+Y G S+ Q +R+NVY+NEA+ GKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 188 PRAVLVDLXPGTMXAVTSXPYGSCSGP 268
           PRAVLVDL PGTM AV S  +G+   P
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRP 87



 Score = 46.8 bits (106), Expect = 2e-06
 Identities = 19/38 (50%), Positives = 23/38 (60%)
 Frame = +1

Query: 256 LFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369
           LFRP N ++G SGAGN WA  HY  GA    +   V+R
Sbjct: 84  LFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121



 Score = 36.3 bits (80), Expect = 0.003
 Identities = 16/24 (66%), Positives = 16/24 (66%)
 Frame = +3

Query: 417 HSLGGGTGXXXGTLXLSNXXXEYP 488
           HSLGGGTG   GTL LS    EYP
Sbjct: 137 HSLGGGTGSGMGTLLLSKIREEYP 160


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 85.0 bits (201), Expect = 6e-18
 Identities = 36/86 (41%), Positives = 56/86 (65%)
 Frame = +2

Query: 11  REIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYVP 190
           REI+ +QAGQCGNQIGS+FW+ +  EHG+   G  +  +   +DR +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 191 RAVLVDLXPGTMXAVTSXPYGSCSGP 268
           RA+L+DL P  +  + S  YGS   P
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNP 88



 Score = 27.9 bits (59), Expect = 1.0
 Identities = 11/35 (31%), Positives = 15/35 (42%)
 Frame = +3

Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521
           HS+ GGTG   G+  L      YP       + +P
Sbjct: 139 HSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173



 Score = 24.6 bits (51), Expect = 9.6
 Identities = 11/25 (44%), Positives = 13/25 (52%), Gaps = 2/25 (8%)
 Frame = +1

Query: 244 TLRKLFRPXNFVF--GXSGAGNNWA 312
           T   L+ P N +      GAGNNWA
Sbjct: 81  TYGSLYNPENILITKNGGGAGNNWA 105


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 70.1 bits (164), Expect = 2e-13
 Identities = 32/83 (38%), Positives = 46/83 (55%), Gaps = 2/83 (2%)
 Frame = +2

Query: 8   MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTGVYKGESDLQLD--RINVYYNEASSGK 181
           MREI+ +  GQ G QIG+  WE+   EHG+   G    E+  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 182 YVPRAVLVDLXPGTMXAVTSXPY 250
           YVPR++ VDL P  +  V + PY
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPY 83



 Score = 34.7 bits (76), Expect = 0.009
 Identities = 16/40 (40%), Positives = 18/40 (45%)
 Frame = +1

Query: 250 RKLFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369
           R LF P   + G   A NN+A  HY  G   V   T  IR
Sbjct: 84  RDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIR 123



 Score = 30.3 bits (65), Expect = 0.19
 Identities = 14/35 (40%), Positives = 15/35 (42%)
 Frame = +3

Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521
           HS GGGTG   G L L     EY        + YP
Sbjct: 139 HSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 66.5 bits (155), Expect = 2e-12
 Identities = 34/88 (38%), Positives = 48/88 (54%), Gaps = 7/88 (7%)
 Frame = +2

Query: 8   MREIVHVQAGQCGNQIGSKFWEIISDEHGVDTTG-------VYKGESDLQLDRINVYYNE 166
           MRE++ V  GQ G QIG+  WE+   EHG+   G       V+K  S L  D    +++E
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLN-DGFGTFFSE 59

Query: 167 ASSGKYVPRAVLVDLXPGTMXAVTSXPY 250
              GK+VPR++ VDL P  +  V + PY
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPY 87



 Score = 32.3 bits (70), Expect = 0.048
 Identities = 15/40 (37%), Positives = 18/40 (45%)
 Frame = +1

Query: 250 RKLFRPXNFVFGXSGAGNNWAXXHYXXGAXXVSSXTSVIR 369
           + LF P   V G   A NN+A  HY  G   + S    IR
Sbjct: 88  KDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127



 Score = 29.9 bits (64), Expect = 0.26
 Identities = 14/35 (40%), Positives = 15/35 (42%)
 Frame = +3

Query: 417 HSLGGGTGXXXGTLXLSNXXXEYPXXXXNXLAXYP 521
           HS GGGTG   G L L     EY        + YP
Sbjct: 143 HSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177


>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 918

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 19/68 (27%), Positives = 35/68 (51%), Gaps = 7/68 (10%)
 Frame = +2

Query: 74  IISDEHGVDTTGVYKGESDLQLDRINVYYNEASSGKYV--PRAVL----VDLXPG-TMXA 232
           I+  +HG+D  G + G+S  +++ +   Y++  +  Y+  P  VL    VDL P  ++  
Sbjct: 281 IVMGDHGMDNKGNHGGDSFDEINSVLWMYSKKPTFGYLKQPGKVLSANQVDLVPTLSLLL 340

Query: 233 VTSXPYGS 256
               PYG+
Sbjct: 341 GNPIPYGN 348


>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 24.6 bits (51), Expect = 9.6
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = +1

Query: 19  SPRASRPMRESDRIEVLGDNLR 84
           SP++  P+ +S  +  LG+NL+
Sbjct: 242 SPKSENPLNQSSFLSFLGENLK 263


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,531,922
Number of Sequences: 5004
Number of extensions: 24146
Number of successful extensions: 53
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 51
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 225926624
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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