Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbpv0303
         (825 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   175   5e-45
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   103   4e-23
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   100   2e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    87   2e-18
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    30   0.35 
SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33 |Schizo...    25   9.9  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  175 bits (427), Expect = 5e-45
 Identities = 87/137 (63%), Positives = 101/137 (73%)
 Frame = +3

Query: 255 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQXGAGNNWAKGHYTEGAELVDFSQRVFF 434
           RA+LVDLEPGTMD+V+SG FG +FRPDN ++ Q GAGN WAKGHYTEGAEL D    V  
Sbjct: 62  RAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDV-- 119

Query: 435 *TSFERKRNRVIASRDFQLTHSLXGAPVPVXGXLLISKIREEYPDRIMXTYSVVPSPKVS 614
                R+     A + FQLTHSL G      G LL+SKIREEYPDR+M T+SV P+PK S
Sbjct: 120 ---VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSS 176

Query: 615 DTVVEPHNATLSVHQLV 665
           DTVVEP+NATLS+HQLV
Sbjct: 177 DTVVEPYNATLSMHQLV 193



 Score = 91.1 bits (216), Expect = 2e-19
 Identities = 39/60 (65%), Positives = 46/60 (76%)
 Frame = +1

Query: 73  MREIVHIQAGQCGNQIGAKFWEIISDXXGIXPXGAYHGDSDLQLERINVYYNEASXGKXV 252
           MREIVHIQAGQCGNQ+GA FW  I+D  G+   G YHG S+ Q ER+NVY+NEA+ GK V
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60



 Score = 27.5 bits (58), Expect = 2.4
 Identities = 16/35 (45%), Positives = 20/35 (57%)
 Frame = +1

Query: 664 LENTDGTYCFRQRRLSTISAFRTLKTFPXPHYXDL 768
           +EN+D T+C     LS+I A  TLK    P Y DL
Sbjct: 193 VENSDETFCIDNEALSSIFA-NTLK-IKSPSYDDL 225


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  103 bits (246), Expect = 4e-23
 Identities = 55/157 (35%), Positives = 85/157 (54%), Gaps = 1/157 (0%)
 Frame = +3

Query: 255 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQXGAGNNWAKGHYTEGAELVD-FSQRVF 431
           R+I VDLEP  +D VR+GP+  +F P+  V  +  A NN+A+GHYT G E++D   +R+ 
Sbjct: 68  RSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI- 126

Query: 432 F*TSFERKRNRVIASRDFQLTHSLXGAPVPVXGXLLISKIREEYPDRIMXTYSVVPSPKV 611
                 R  +     + F + HS  G      G LL+ ++  EY  +    +SV P+P+V
Sbjct: 127 -----RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181

Query: 612 SDTVVEPHNATLSVHQLVGKHRRXLLFSTTKALYDIC 722
           S +VVEP+N+ L+ H  +       +    +A YDIC
Sbjct: 182 STSVVEPYNSVLTTHATLDNSDCTFMVD-NEACYDIC 217



 Score = 37.1 bits (82), Expect = 0.003
 Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 6/66 (9%)
 Frame = +1

Query: 73  MREIVHIQAGQCGNQIGAKFWEIISDXXGIXPXG------AYHGDSDLQLERINVYYNEA 234
           MRE++ +  GQ G QIG   WE+     GI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 235 SXGKXV 252
             GK V
Sbjct: 61  GQGKFV 66


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  100 bits (240), Expect = 2e-22
 Identities = 54/156 (34%), Positives = 82/156 (52%)
 Frame = +3

Query: 255 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQXGAGNNWAKGHYTEGAELVDFSQRVFF 434
           R+I VDLEP  +D VR+GP+  +F P+  +  +  A NN+A+GHYT G ELVD       
Sbjct: 64  RSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVT---- 119

Query: 435 *TSFERKRNRVIASRDFQLTHSLXGAPVPVXGXLLISKIREEYPDRIMXTYSVVPSPKVS 614
                R  +     + F + HS  G      G LL+ ++  EY  +    +SV P+P+VS
Sbjct: 120 -DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVS 178

Query: 615 DTVVEPHNATLSVHQLVGKHRRXLLFSTTKALYDIC 722
            +VVEP+N+ L+ H  +       +    ++ YDIC
Sbjct: 179 TSVVEPYNSVLTTHATLDLADCTFMVD-NESCYDIC 213



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 22/62 (35%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = +1

Query: 73  MREIVHIQAGQCGNQIGAKFWEIISDXXGIXPXGAYHGDSDLQLE--RINVYYNEASXGK 246
           MREI+ I  GQ G QIG   WE+     GI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 247 XV 252
            V
Sbjct: 61  YV 62


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 87.4 bits (207), Expect = 2e-18
 Identities = 47/139 (33%), Positives = 77/139 (55%), Gaps = 3/139 (2%)
 Frame = +3

Query: 255 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFXQ--XGAGNNWAKGHYTEGAELVDFSQRV 428
           RAIL+DLEP  ++++ S  +G ++ P+N +  +   GAGNNWA G+        D    +
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMDMI 122

Query: 429 FF*TSFERKRNRVIASRDFQLTHSLXGAPVPVXGXLLISKIREEYPDRIMXTYSVVP-SP 605
                 +R+ +   +   F L HS+ G      G  L+ ++ + YP +I+ TYSV P S 
Sbjct: 123 ------DREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176

Query: 606 KVSDTVVEPHNATLSVHQL 662
            VSD VV+P+N+ L++ +L
Sbjct: 177 SVSDVVVQPYNSLLALKRL 195



 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 21/59 (35%), Positives = 37/59 (62%)
 Frame = +1

Query: 76  REIVHIQAGQCGNQIGAKFWEIISDXXGIXPXGAYHGDSDLQLERINVYYNEASXGKXV 252
           REI+ +QAGQCGNQIG++FW+ +    GI P G     +   ++R +V++ ++   + +
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYI 61


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 30.3 bits (65), Expect = 0.35
 Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
 Frame = +3

Query: 306 GPFGQIFRPDNFVFXQXGAG-NNWAKGHYTEGAEL-VDFSQRVFF*TSFERKRNRVIASR 479
           GP+G +F P  F+F   G    NW+   Y E A L  ++    F  ++F     R  A +
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHLDSNWRHGRFLVSTFNNPSIRFPADQ 215

Query: 480 DFQLT 494
           DF+ T
Sbjct: 216 DFRAT 220


>SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 338

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +2

Query: 611 VGHSSRTAQRHTFSSSISWKT 673
           +GHSS+  Q H F  SIS +T
Sbjct: 289 LGHSSKKLQTHPFFCSISLET 309


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,133,572
Number of Sequences: 5004
Number of extensions: 58101
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)