Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbpv0254
(430 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 161 1e-40
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 161 1e-40
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 161 1e-40
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 161 1e-40
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 98 3e-21
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 89 9e-19
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 40 5e-04
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 35 0.027
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.11
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.11
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 1.3
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 28 2.3
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 28 3.1
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 28 3.1
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 28 3.1
At5g37055.1 68418.m04446 zinc finger (HIT type) family protein c... 27 4.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 161 bits (392), Expect = 1e-40
Identities = 84/128 (65%), Positives = 89/128 (69%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA M K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGT 404
DKLKA RE G + + FI NMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFXAGIS 428
G F AGIS
Sbjct: 121 GGFEAGIS 128
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 161 bits (392), Expect = 1e-40
Identities = 84/128 (65%), Positives = 89/128 (69%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA M K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGT 404
DKLKA RE G + + FI NMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFXAGIS 428
G F AGIS
Sbjct: 121 GGFEAGIS 128
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 161 bits (392), Expect = 1e-40
Identities = 84/128 (65%), Positives = 89/128 (69%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA M K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGT 404
DKLKA RE G + + FI NMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFXAGIS 428
G F AGIS
Sbjct: 121 GGFEAGIS 128
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 161 bits (392), Expect = 1e-40
Identities = 84/128 (65%), Positives = 89/128 (69%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA M K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGT 404
DKLKA RE G + + FI NMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFXAGIS 428
G F AGIS
Sbjct: 121 GGFEAGIS 128
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 97.9 bits (233), Expect = 3e-21
Identities = 48/121 (39%), Positives = 68/121 (56%)
Frame = +3
Query: 60 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVLDKLKA 239
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 240 XREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGTGEFXA 419
RE G + L + F+ NMI G +QAD A+L++ A G F A
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 420 G 422
G
Sbjct: 358 G 358
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 89.4 bits (212), Expect = 9e-19
Identities = 45/123 (36%), Positives = 69/123 (56%)
Frame = +3
Query: 54 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVLDKL 233
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 234 KAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGTGEF 413
+ R G + T L + ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 414 XAG 422
G
Sbjct: 218 ETG 220
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 40.3 bits (90), Expect = 5e-04
Identities = 31/122 (25%), Positives = 48/122 (39%)
Frame = +3
Query: 42 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWV 221
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 222 LDKLKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAG 401
+D R G + + ++ NMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 402 TG 407
G
Sbjct: 177 DG 178
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 34.7 bits (76), Expect = 0.027
Identities = 30/119 (25%), Positives = 48/119 (40%)
Frame = +3
Query: 51 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAXXMGKGSFKYAWVLDK 230
+ K H+N+ IGHVD GK+T T I K E+ + +A + +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT-------AAITKVLAEEGKAKAIAFDE--------IDK 107
Query: 231 LKAXREXGSXSXLLSGSSXLAXTMXPSLMLLXTEHFIXNMITGTSQADCAVLIVAAGTG 407
++ G A + ++ NMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDG 166
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.11
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGG 143
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.11
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +3
Query: 57 KTHINIVVIGHVDSGKSTTTGHLIYKCG 140
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.1 bits (62), Expect = 1.3
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCG 140
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 28.3 bits (60), Expect = 2.3
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 167
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 27.9 bits (59), Expect = 3.1
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +3
Query: 33 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 152
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 27.9 bits (59), Expect = 3.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGI 146
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 27.9 bits (59), Expect = 3.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGI 146
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At5g37055.1 68418.m04446 zinc finger (HIT type) family protein
contains Pfam profile: PF04438 HIT zinc finger
Length = 171
Score = 27.5 bits (58), Expect = 4.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -2
Query: 222 IPKHI*RILYPXXGPPSRTSRWYVC 148
+P H+ L GPPS +SR Y C
Sbjct: 110 LPSHVPTYLKAAVGPPSSSSRRYFC 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,472,780
Number of Sequences: 28952
Number of extensions: 112121
Number of successful extensions: 248
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 675111616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -