BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0232 (767 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 107 1e-24 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 55 1e-08 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 55 1e-08 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 52 1e-07 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 44 3e-05 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 6e-05 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 42 7e-05 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 35 0.011 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 35 0.015 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 35 0.015 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 35 0.015 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 33 0.034 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 107 bits (258), Expect = 1e-24 Identities = 49/137 (35%), Positives = 78/137 (56%), Gaps = 1/137 (0%) Frame = +3 Query: 63 KRLMREAIELAEATEEYC-ARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYXXX 239 KRLM+E + L + E A+P +N+ EWH+ + GP T +EGG YHG ++ P +Y Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67 Query: 240 XXXXXXXXXNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTPAEGRIGSLD 419 +GRF+ N ++CLS S HP++W PSW + T L+ L++FM T E G + Sbjct: 68 PPAIRMITPSGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFM-TSDEITTGGIV 126 Query: 420 YTPAERKILAKKSTNWS 470 + + R+ AK + ++ Sbjct: 127 TSESTRRTYAKDTKRFN 143 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 55.2 bits (127), Expect = 1e-08 Identities = 36/113 (31%), Positives = 55/113 (48%), Gaps = 3/113 (2%) Frame = +3 Query: 63 KRLMREAIELA-EATEEYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYXXX 239 KR+++E L + A P EDNL + T++GP+ + +EGG +H + LP EY Sbjct: 5 KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMM 64 Query: 240 XXXXXXXXXNGRFEINK--KICLSISGHHPETWQPSWSIRTALLALIAFMPTP 392 ++K +ICLS + W P+ IRT LL++ A M P Sbjct: 65 PPNVRFLTKIYHPNVDKLGRICLSTL---KKDWSPALQIRTVLLSIQALMGAP 114 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 54.8 bits (126), Expect = 1e-08 Identities = 40/139 (28%), Positives = 62/139 (44%), Gaps = 14/139 (10%) Frame = +3 Query: 45 TKCPGVKRLMREAIELAE-ATEEYCARPL-EDNLFEWHFTVQGPKGTEFEGGIYHGRILL 218 +K ++RLM+E EL E + A P ED+ F W +QGP GT FEGG+Y + Sbjct: 2 SKAMALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKF 61 Query: 219 PKEYXXXXXXXXXXXXNGRFEINK--KICLSI---SGHHP-------ETWQPSWSIRTAL 362 P +Y + K +C+SI G P E W P S+ L Sbjct: 62 PSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKIL 121 Query: 363 LALIAFMPTPAEGRIGSLD 419 L++++ + P + ++D Sbjct: 122 LSVMSMLAEPNDESGANID 140 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 51.6 bits (118), Expect = 1e-07 Identities = 31/114 (27%), Positives = 51/114 (44%), Gaps = 3/114 (2%) Frame = +3 Query: 60 VKRLMREAIELAEATEEYC-ARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY-- 230 +KR+ RE +L + C A P+ D+LF W T+ GP + + GG++ I P +Y Sbjct: 3 LKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPF 62 Query: 231 XXXXXXXXXXXXNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 392 + N ICL I + W P+ +I LL++ + + P Sbjct: 63 KPPKVNFTTRIYHPNINSNGSICLDIL---RDQWSPALTISKVLLSICSLLTDP 113 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 43.6 bits (98), Expect = 3e-05 Identities = 27/110 (24%), Positives = 50/110 (45%), Gaps = 13/110 (11%) Frame = +3 Query: 129 EDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY--XXXXXXXXXXXXNGRFEINKKICL 302 + ++FEW + GP+ T +EGG +H + P++Y + N ++C+ Sbjct: 32 DKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCI 91 Query: 303 SISGHHP-----------ETWQPSWSIRTALLALIAFMPTPAEGRIGSLD 419 SI H P E W P S T L+++I+ + +P + ++D Sbjct: 92 SIL-HPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSPNDESPANID 140 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 42.7 bits (96), Expect = 6e-05 Identities = 29/114 (25%), Positives = 55/114 (48%), Gaps = 6/114 (5%) Frame = +3 Query: 60 VKRLMRE--AIELAEATEEYCARPLED-NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY 230 +KRL +E + + + + +P+ D ++F W ++GP T +EGG + I + + Y Sbjct: 5 IKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGY 64 Query: 231 XXXXXXXXXXXXNGRFEI---NKKICLSISGHHPETWQPSWSIRTALLALIAFM 383 I N ++C+ I H W P+WS+++A LA+I+ + Sbjct: 65 PISPPSVYFQTKIVHPNISWTNGEVCMDILKTH---WSPAWSLQSACLAIISLL 115 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 42.3 bits (95), Expect = 7e-05 Identities = 29/123 (23%), Positives = 55/123 (44%), Gaps = 5/123 (4%) Frame = +3 Query: 63 KRLMREAIELAE-ATEEYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY--X 233 +RL +E +L E + ++DNLF W T GP + + GG +H + P +Y Sbjct: 10 RRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQ 69 Query: 234 XXXXXXXXXXXNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFM--PTPAEGRI 407 + F+ +CL+I + ++PS +R+ L ++ + P P + + Sbjct: 70 PPTIEFTTRIYHPNFDSEGNVCLAIL--KQQVFKPSIKLRSVLEQILQLLREPNPDDPLV 127 Query: 408 GSL 416 S+ Sbjct: 128 ASI 130 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 35.1 bits (77), Expect = 0.011 Identities = 29/116 (25%), Positives = 54/116 (46%), Gaps = 7/116 (6%) Frame = +3 Query: 66 RLMREAIELAEATEEYCAR----PLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYX 233 R R A ELA+ ++ A + D++ +GP+GT +EGG + I +P +Y Sbjct: 5 RSRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYP 64 Query: 234 XXXXXXXXXXXNGRFEINKK---ICLSISGHHPETWQPSWSIRTALLALIAFMPTP 392 ++ + ICL I + W P +++++AL++L + + TP Sbjct: 65 FRPPKMNFDTKIYHPNVSSQTGAICLDIL---KDQWSPVYTMKSALISLQSLLCTP 117 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 34.7 bits (76), Expect = 0.015 Identities = 22/88 (25%), Positives = 38/88 (43%), Gaps = 2/88 (2%) Frame = +3 Query: 135 NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY--XXXXXXXXXXXXNGRFEINKKICLSI 308 +L W + G T +EGG+Y + P+EY + + +CLSI Sbjct: 37 DLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSI 96 Query: 309 SGHHPETWQPSWSIRTALLALIAFMPTP 392 + E W+P+ +I+ LL + + P Sbjct: 97 L-NEEEGWKPAITIKQILLGIQDLLDDP 123 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 34.7 bits (76), Expect = 0.015 Identities = 24/113 (21%), Positives = 47/113 (41%), Gaps = 3/113 (2%) Frame = +3 Query: 63 KRLMREAIELAEATEE-YCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY--X 233 +RLMR+ + + A P+ DN+ W+ + GP T FE G + + ++Y Sbjct: 7 RRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNK 66 Query: 234 XXXXXXXXXXXNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 392 + N ++CL I + W P++ + L ++ + + P Sbjct: 67 PPLVKFVSTMFHPNVYANGELCLDILQNR---WSPTYDVAAILTSIQSLLNDP 116 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 34.7 bits (76), Expect = 0.015 Identities = 30/114 (26%), Positives = 48/114 (42%), Gaps = 4/114 (3%) Frame = +3 Query: 63 KRLMREAIELAEA-TEEYCARPLED-NLFEWHFTVQGPKGTEFEGGIYHGRILLPKE--Y 230 KRL E + L + T A P D NL W T+ GP T +EG + + P Y Sbjct: 31 KRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPY 90 Query: 231 XXXXXXXXXXXXNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 392 + +++ ICL I + W ++++T LL+L + + P Sbjct: 91 SPPTIIFTSPMWHPNVDMSGNICLDIL---KDKWSAVYNVQTILLSLQSLLGEP 141 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 33.5 bits (73), Expect = 0.034 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +3 Query: 108 EYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY 230 +Y + DN+ E++ GP T + GGI+ + LP EY Sbjct: 19 DYEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEY 59 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,768,593 Number of Sequences: 5004 Number of extensions: 51250 Number of successful extensions: 129 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 124 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 369323696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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