BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0215 (557 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 165 5e-42 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 90 2e-19 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 89 4e-19 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 89 4e-19 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 62 4e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 58 9e-10 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 48 1e-06 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 37 0.002 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.087 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.3 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.3 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 10.0 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 10.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 165 bits (400), Expect = 5e-42 Identities = 73/79 (92%), Positives = 75/79 (94%) Frame = +1 Query: 271 EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 450 EAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NR Sbjct: 57 EAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNR 116 Query: 451 EKMTQIMFETFNTPAMYVA 507 EKMTQI+FETFN PA YVA Sbjct: 117 EKMTQIIFETFNAPAFYVA 135 Score = 99 bits (238), Expect = 2e-22 Identities = 43/49 (87%), Positives = 47/49 (95%) Frame = +2 Query: 107 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGXK 253 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMG K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQK 50 Score = 34.3 bits (75), Expect = 0.012 Identities = 16/16 (100%), Positives = 16/16 (100%) Frame = +3 Query: 510 QAVLSLYASGRTTGIV 557 QAVLSLYASGRTTGIV Sbjct: 137 QAVLSLYASGRTTGIV 152 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 89.8 bits (213), Expect = 2e-19 Identities = 39/83 (46%), Positives = 57/83 (68%), Gaps = 1/83 (1%) Frame = +1 Query: 271 EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKAN 447 EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N Sbjct: 61 EAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRN 120 Query: 448 REKMTQIMFETFNTPAMYVATKP 516 +EK+ + +ET N PA+ + +P Sbjct: 121 KEKIAEYFYETLNVPALSFSLQP 143 Score = 55.2 bits (127), Expect = 6e-09 Identities = 22/37 (59%), Positives = 29/37 (78%) Frame = +2 Query: 125 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 235 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48 Score = 28.7 bits (61), Expect = 0.61 Identities = 13/16 (81%), Positives = 14/16 (87%) Frame = +3 Query: 510 QAVLSLYASGRTTGIV 557 Q VL+LYAS RTTGIV Sbjct: 142 QPVLALYASARTTGIV 157 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 89.0 bits (211), Expect = 4e-19 Identities = 36/72 (50%), Positives = 47/72 (65%) Frame = +1 Query: 298 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 477 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 478 TFNTPAMYVATK 513 +FN +Y+A + Sbjct: 149 SFNCAGLYIAVQ 160 Score = 40.7 bits (91), Expect = 1e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +2 Query: 125 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 208 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 89.0 bits (211), Expect = 4e-19 Identities = 35/79 (44%), Positives = 58/79 (73%) Frame = +1 Query: 271 EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 450 EA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANR Sbjct: 57 EAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANR 116 Query: 451 EKMTQIMFETFNTPAMYVA 507 EKM + MFE + +YVA Sbjct: 117 EKMCETMFERYGFGGVYVA 135 Score = 48.0 bits (109), Expect = 9e-07 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +2 Query: 113 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 217 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 Score = 26.2 bits (55), Expect = 3.3 Identities = 11/16 (68%), Positives = 14/16 (87%) Frame = +3 Query: 510 QAVLSLYASGRTTGIV 557 QAVLSLYA G ++G+V Sbjct: 137 QAVLSLYAQGLSSGVV 152 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 62.5 bits (145), Expect = 4e-11 Identities = 28/71 (39%), Positives = 40/71 (56%) Frame = +1 Query: 295 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 474 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 475 ETFNTPAMYVA 507 E+ PA Y+A Sbjct: 123 ESLRCPATYLA 133 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 110 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 211 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 58.0 bits (134), Expect = 9e-10 Identities = 24/63 (38%), Positives = 40/63 (63%) Frame = +1 Query: 310 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 489 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 490 PAM 498 P++ Sbjct: 145 PSV 147 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 125 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 220 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 47.6 bits (108), Expect = 1e-06 Identities = 18/62 (29%), Positives = 36/62 (58%) Frame = +1 Query: 319 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 498 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 499 YV 504 Y+ Sbjct: 134 YL 135 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 110 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 211 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 36.7 bits (81), Expect = 0.002 Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 4/73 (5%) Frame = +1 Query: 310 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 477 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 478 TFNTPAMYVATKP 516 P +A +P Sbjct: 172 ECQVPGFTIAYEP 184 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.5 bits (68), Expect = 0.087 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Frame = +1 Query: 370 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 492 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.3 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 412 LLTEAPLNPKANREKMTQIMFETFN 486 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 4.3 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -1 Query: 152 TYRSHCLLPTRQLLRRTS*ISEI 84 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 10.0 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +2 Query: 125 LVVDNGSGMCKAGFAG 172 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 24.6 bits (51), Expect = 10.0 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = +2 Query: 170 GDDAPRAVFPSIVGRPRHQGVMVGM 244 G+ PRA F ++ P H G+++ M Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM 526 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,339,853 Number of Sequences: 5004 Number of extensions: 48112 Number of successful extensions: 135 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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