BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0188 (854 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 61 2e-10 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 52 8e-08 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.16 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.64 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 28 1.5 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 28 1.5 SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||M... 28 1.5 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 2.6 SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo... 27 2.6 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.6 SPBC31F10.16 |||ChAPs family protein|Schizosaccharomyces pombe|c... 27 4.5 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 27 4.5 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 5.9 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 7.8 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 26 7.8 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 60.9 bits (141), Expect = 2e-10 Identities = 29/68 (42%), Positives = 43/68 (63%) Frame = +2 Query: 344 LKENGIQASKDIKLLLLGNCESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTSSRSF 523 +K N DIK+LLLG +SGK+TI+KQM++++ GF+ KQYR +++ N S Sbjct: 64 VKGNSTSGGNDIKVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLC 123 Query: 524 AILRAMPN 547 +L AM N Sbjct: 124 LLLEAMDN 131 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 52.4 bits (120), Expect = 8e-08 Identities = 26/77 (33%), Positives = 47/77 (61%) Frame = +2 Query: 332 IDKNLKENGIQASKDIKLLLLGNCESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTS 511 I+K ++ + K K+LLLG +SGKSTI KQ+KI++++GF+ E+ + PV+ N Sbjct: 20 IEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIMTFIPVIRRNLL 79 Query: 512 SRSFAILRAMPNFGHHL 562 + +++ + G +L Sbjct: 80 ESAKTLVKIIVQKGINL 96 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 31.5 bits (68), Expect = 0.16 Identities = 19/56 (33%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = -3 Query: 318 AARCTLFGRLGAPHIGSSPYARPQAAPTPEPGA-LSPISQHALYTRPVAPALPEPP 154 A LFG + H S+P RPQ+A P+ A P ++ P AP +P P Sbjct: 1672 ALAAKLFGGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGP 1727 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.5 bits (63), Expect = 0.64 Identities = 15/46 (32%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Frame = -3 Query: 285 APHIGSSPYARPQAA--PTPEPGALSPISQHALYTRPVAPALPEPP 154 AP G P +P A P P P P+ + ++ PV P PP Sbjct: 1173 APSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218 Score = 26.2 bits (55), Expect = 5.9 Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 5/51 (9%) Frame = -3 Query: 273 GSSPYARPQAA--PTPEPGALSPISQHALYTRPV-AP--ALPEPPYTILAP 136 G+ P +P A P P P P+ + ++ PV AP A P P ++ AP Sbjct: 1119 GAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAP 1169 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 28.3 bits (60), Expect = 1.5 Identities = 15/27 (55%), Positives = 17/27 (62%) Frame = -2 Query: 355 ILFQVLIDQFASSSPLHALRPTGRTPY 275 ILF LI +SSS LH L P+G PY Sbjct: 183 ILFNALISILSSSSTLHKLYPSG-LPY 208 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 28.3 bits (60), Expect = 1.5 Identities = 19/50 (38%), Positives = 27/50 (54%) Frame = -3 Query: 285 APHIGSSPYARPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAP 136 AP + S+P RP A PE A S + A+ P A ++P+PP +AP Sbjct: 538 APEVPSAPQ-RPAAPVVPE--APSVPQRPAVPVVPEALSVPQPPVAPVAP 584 Score = 26.6 bits (56), Expect = 4.5 Identities = 15/53 (28%), Positives = 22/53 (41%) Frame = -3 Query: 294 RLGAPHIGSSPYARPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAP 136 R AP + +P + A P ALS P P++P+PP + P Sbjct: 547 RPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVP 599 Score = 25.8 bits (54), Expect = 7.8 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = -3 Query: 294 RLGAPHIGSSPYA-RPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAP 136 R P + +P +P AAP P S + A+ P AP++P+PP + P Sbjct: 622 RPAVPVVPEAPSVPQPPAAPVV-PEVPSVPQRPAVPVVPEAPSVPQPPAAPVVP 674 >SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 28.3 bits (60), Expect = 1.5 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +3 Query: 690 AVQGXPWVRFPTGYPTPTNFPLKLFPWD 773 +V G + +FPT YP P + L WD Sbjct: 10 SVSGDKYPQFPTDYPVPLRAYVNLEDWD 37 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 27.5 bits (58), Expect = 2.6 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +2 Query: 359 IQASKDIKLLLLGNCESGKSTIVKQMKIIHESGFTNE 469 I I + +G+ GKST+VK + +H F NE Sbjct: 18 ISRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNE 54 >SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizosaccharomyces pombe|chr 2|||Manual Length = 589 Score = 27.5 bits (58), Expect = 2.6 Identities = 24/93 (25%), Positives = 47/93 (50%), Gaps = 6/93 (6%) Frame = +2 Query: 284 APSRPKSVQRAARSKLIDK--NLKENGIQASKDI--KLLLLGNCESGKSTIVKQMKIIHE 451 +P+ PK R+ S +LK++ +A + + +LL G+C+ ++ + H+ Sbjct: 7 SPASPKPTSRSRESSRSPSVTDLKDHS-KAKRTLCRNILLYGSCKHSENGCAFR----HD 61 Query: 452 SGF--TNEDFKQYRPVVYSNTSSRSFAILRAMP 544 F ++E+ +QY N +S SF +RA+P Sbjct: 62 GPFIPSSENLEQYSVKKKLNAASASFQPVRALP 94 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 27.5 bits (58), Expect = 2.6 Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 4/45 (8%) Frame = -3 Query: 270 SSPYARPQAAPTPEPGALSPISQHALYTRPV----APALPEPPYT 148 +SP +PQ P P A +P +H PV +P++P P T Sbjct: 719 NSPAIKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAPLT 763 >SPBC31F10.16 |||ChAPs family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 679 Score = 26.6 bits (56), Expect = 4.5 Identities = 10/28 (35%), Positives = 15/28 (53%), Gaps = 1/28 (3%) Frame = -1 Query: 578 SVSIAIDDAQSWAWHAR-WRTSDWTYCC 498 ++S ++D +Q W WR S YCC Sbjct: 107 TLSYSLDKSQQWFGKGNAWRVSHGVYCC 134 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 26.6 bits (56), Expect = 4.5 Identities = 23/78 (29%), Positives = 39/78 (50%), Gaps = 10/78 (12%) Frame = +2 Query: 266 ELPIWGAPSRPKSVQRAARSKLIDKN---------LKENGIQA-SKDIKLLLLGNCESGK 415 E P W +R ++ R+ L K L++ ++A SK+ L+LLG SGK Sbjct: 480 ETPSWRQATRNANISYGKRTTLSMKEQREGLPVFKLRKQFLEAVSKNQILVLLGETGSGK 539 Query: 416 STIVKQMKIIHESGFTNE 469 +T + Q + E G+T++ Sbjct: 540 TTQITQ--YLAEEGYTSD 555 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 26.2 bits (55), Expect = 5.9 Identities = 13/42 (30%), Positives = 17/42 (40%) Frame = -3 Query: 273 GSSPYARPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYT 148 G A P A P P P ++ + A + V P PP T Sbjct: 882 GGEALAAPVAVPAPIPAPVAEPAPPAAPAKEVVEKAPSPPAT 923 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.8 bits (54), Expect = 7.8 Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 2/38 (5%) Frame = -3 Query: 240 PTPEPGALSPISQHALYTRPVAPALPEPPY--TILAPN 133 P P PG + A T P AP +P P+ TI A N Sbjct: 290 PPPPPGMVMASPSPAAATIPGAPVMPNIPFYQTINAQN 327 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 25.8 bits (54), Expect = 7.8 Identities = 9/26 (34%), Positives = 22/26 (84%) Frame = +2 Query: 365 ASKDIKLLLLGNCESGKSTIVKQMKI 442 ++K++++L+LG +GK+TI+ ++K+ Sbjct: 18 SNKEMRILMLGLDAAGKTTILYKLKL 43 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,358,788 Number of Sequences: 5004 Number of extensions: 68284 Number of successful extensions: 250 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 228 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 247 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 424464280 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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