BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0153 (724 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 62 1e-10 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 33 0.055 SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 32 0.072 SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 29 0.51 SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.89 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.2 SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c... 27 2.1 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 3.6 SPAC1610.01 ||SPAC17A5.17|conserved fungal protein|Schizosacchar... 26 6.3 SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 6.3 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 6.3 SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 6.3 SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 8.3 SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 25 8.3 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 61.7 bits (143), Expect = 1e-10 Identities = 33/81 (40%), Positives = 47/81 (58%), Gaps = 1/81 (1%) Frame = +2 Query: 257 ARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 433 A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +M Y I Sbjct: 98 AAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGEMSNSIDYRI 156 Query: 434 MFGPDICGPGTKKVHVIFSYK 496 MFGPD CG +VH IF +K Sbjct: 157 MFGPDKCGV-NDRVHFIFKHK 176 Score = 37.1 bits (82), Expect = 0.003 Identities = 16/26 (61%), Positives = 20/26 (76%) Frame = +1 Query: 547 THLYTLIVKPDNTYEVLIDNEKVESG 624 T+LYTLIVKPD T+EV I+ + V G Sbjct: 201 TNLYTLIVKPDQTFEVRINGDVVRQG 226 Score = 36.7 bits (81), Expect = 0.003 Identities = 15/24 (62%), Positives = 17/24 (70%) Frame = +3 Query: 651 LPPKKIKDPEAKKPEDWDDKPTIP 722 LPP +I DPE KP DW D+P IP Sbjct: 237 LPPVEIYDPEDIKPADWVDEPEIP 260 Score = 34.7 bits (76), Expect = 0.014 Identities = 13/18 (72%), Positives = 15/18 (83%) Frame = +3 Query: 657 PKKIKDPEAKKPEDWDDK 710 P I DPEA+KPEDWDD+ Sbjct: 294 PLYIPDPEAQKPEDWDDE 311 Score = 31.1 bits (67), Expect = 0.17 Identities = 12/20 (60%), Positives = 16/20 (80%), Gaps = 1/20 (5%) Frame = +3 Query: 657 PKKIKDPEAKKPEDW-DDKP 713 P+ I DP+A KPEDW +D+P Sbjct: 275 PRMIPDPDAVKPEDWLEDEP 294 Score = 29.5 bits (63), Expect = 0.51 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +3 Query: 645 DFLPPKKIKDPEAKKPEDWDD 707 D++ +I DP A KP+DWD+ Sbjct: 252 DWVDEPEIPDPNAVKPDDWDE 272 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 32.7 bits (71), Expect = 0.055 Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 4/77 (5%) Frame = -1 Query: 271 SIEPRISEVF---KPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNT 104 S+ P +E + + S+S SL P VN + S + ++ L S + S+ NF+S ++ Sbjct: 467 SVTPTSAENYTSSEAFSTSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASS 526 Query: 103 SQFIEDNASKLTTTSTT 53 S + + S L+T STT Sbjct: 527 SLSLTNAKSSLSTPSTT 543 >SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 32.3 bits (70), Expect = 0.072 Identities = 21/64 (32%), Positives = 29/64 (45%) Frame = +2 Query: 311 PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFS 490 P+V+ F VK + CG Y+ + + K++ E P I FG CG I S Sbjct: 103 PIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKKCGMFDYISLSIIS 161 Query: 491 YKGK 502 Y GK Sbjct: 162 YDGK 165 >SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 29.5 bits (63), Expect = 0.51 Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 3/43 (6%) Frame = -1 Query: 289 FELTGESIEPRIS---EVFKPLSSSGSLKNFPAVNLNFPNSFP 170 F+LTGES S E F+P + SL N P +N ++ ++ P Sbjct: 674 FKLTGESSNGSNSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716 >SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 28.7 bits (61), Expect = 0.89 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 651 LPPKKIKDPEAKKPEDWDDKPTIP 722 LP + K P AKKPED++ P P Sbjct: 168 LPSPEYKAPPAKKPEDYEASPGEP 191 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 28.3 bits (60), Expect = 1.2 Identities = 15/44 (34%), Positives = 19/44 (43%) Frame = -1 Query: 196 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 65 N N F + + S +SGNFS T Q DN S +T Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618 >SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 801 Score = 27.5 bits (58), Expect = 2.1 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +1 Query: 583 TYEVLIDNEKVESGRPRGRLGTSFRLRKSRTLKPR 687 TY +LID +++ GR GRLG K + KP+ Sbjct: 642 TYILLIDT-RIKEGRYAGRLGFERNFAKDKKPKPK 675 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 26.6 bits (56), Expect = 3.6 Identities = 24/103 (23%), Positives = 42/103 (40%), Gaps = 2/103 (1%) Frame = -1 Query: 379 DLEVSASTVNVLFMFDSELDYQGFTLITERFELTGESIEPRISEVFKPLSSSGSLKNFPA 200 DLE ++ F D ++D I +IEP S V +++S S ++ Sbjct: 15 DLEGRHDSIRKSFEAD-DIDVTDENTIFSTSRKEQSTIEPMDSFVENNIANSSSQQSVSE 73 Query: 199 VNLNFPNSFP--GCSLYTQLLSHESSGNFSSKNTSQFIEDNAS 77 +++ P+S P G + L+ S N N I D ++ Sbjct: 74 IDIQLPSSEPKEGVKEESNLIESNSLDNVQEINPQNTIGDKSA 116 >SPAC1610.01 ||SPAC17A5.17|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 217 Score = 25.8 bits (54), Expect = 6.3 Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 2/46 (4%) Frame = -1 Query: 286 ELTGESIEPRISEVFKPLSSSGSLKNFPAV--NLNFPNSFPGCSLY 155 +L GE+I P + + K SSG+L P+ F + F +LY Sbjct: 96 QLEGEAIRPLLDHILKFERSSGTLHEAPSTEDENEFTDDFLELTLY 141 >SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosaccharomyces pombe|chr 2|||Manual Length = 297 Score = 25.8 bits (54), Expect = 6.3 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = +3 Query: 114 EEKFPDDSWESNWVYS 161 EEKFPD +W +W S Sbjct: 251 EEKFPDIAWRVSWSLS 266 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 25.8 bits (54), Expect = 6.3 Identities = 9/39 (23%), Positives = 20/39 (51%) Frame = +3 Query: 117 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDD 233 + F +D + +W EH K ++ + + FS+ +D+ Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSEGDDE 87 >SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 457 Score = 25.8 bits (54), Expect = 6.3 Identities = 11/26 (42%), Positives = 15/26 (57%), Gaps = 1/26 (3%) Frame = +3 Query: 648 FLPPKKIKDPEAKKPEDWDD-KPTIP 722 F+PP +I PE K +WD+ P P Sbjct: 100 FIPPARILFPEEKLSMEWDNIMPNAP 125 >SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1315 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/31 (38%), Positives = 14/31 (45%) Frame = -2 Query: 159 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 67 C P FP N+QE H+ I AS Q Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821 >SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosaccharomyces pombe|chr 2|||Manual Length = 948 Score = 25.4 bits (53), Expect = 8.3 Identities = 31/129 (24%), Positives = 45/129 (34%), Gaps = 5/129 (3%) Frame = -1 Query: 424 WSLSVHVLLLQFAVKDLEVSASTVNVLFMFDSELDYQGFTLITERFELTGESIEPRISEV 245 +S V L + E+S+ N GF I + S +I Sbjct: 359 YSTGVETLYETETITSSEISSIIYNFSTPISGSSFPDGFKPINPTSFPSLTSSTKKIPST 418 Query: 244 FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-----SSGNFSSKNTSQFIEDNA 80 P SS P+V+ N +SF S +T H SS +S N S +A Sbjct: 419 TLPTSSKMITTTTPSVSNNTQSSFLIISTFTSSYEHSEPFKVSSVPLTSNNFSSISHSSA 478 Query: 79 SKLTTTSTT 53 S L T ++ Sbjct: 479 SSLPITPSS 487 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,226,037 Number of Sequences: 5004 Number of extensions: 70489 Number of successful extensions: 229 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 228 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 339215786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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